Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS31054
(701 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 83 5e-17
SPAC16E8.16 |||transcription factor TFIIB |Schizosaccharomyces p... 30 0.37
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 2.0
SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces po... 27 2.6
SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos... 27 2.6
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 27 3.4
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 27 3.4
SPCC162.11c |||uridine kinase |Schizosaccharomyces pombe|chr 3||... 25 7.9
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 82.6 bits (195), Expect = 5e-17
Identities = 40/91 (43%), Positives = 55/91 (60%)
Frame = +3
Query: 255 CVYMTCRTEGTPHLLIDVSDAVPLCCYQLGRAYFKLSRALCINIPPTDPCLYILRFASQL 434
C+Y+ CR T H+LID SD + + ++LG + KL R L N+P DP LYI RFAS L
Sbjct: 139 CLYIVCRISKTSHMLIDFSDILQINVFKLGSTFLKLCRVLRPNLPLLDPSLYISRFASLL 198
Query: 435 KFEDKQHEVSMTALRLDQRMKKDSITRGEDP 527
+F + H V+ A+RL RM +D + G P
Sbjct: 199 EFGPETHRVANDAIRLVARMNRDWMQIGRRP 229
Score = 55.2 bits (127), Expect = 9e-09
Identities = 27/69 (39%), Positives = 42/69 (60%)
Frame = +2
Query: 431 VEIRRQTTRGQHDCTQTGSANEEGLHHSGRRPSGICGAALLIAARLHEFSRTPSDIVRIV 610
+E +T R +D + + GRRP+GICGA LLIAAR++ F R+ ++V +V
Sbjct: 198 LEFGPETHRVANDAIRLVARMNRDWMQIGRRPAGICGACLLIAARMNNFRRSVREVVHVV 257
Query: 611 KVHESTLRK 637
KV + T++K
Sbjct: 258 KVADITIQK 266
Score = 40.7 bits (91), Expect = 2e-04
Identities = 26/78 (33%), Positives = 42/78 (53%), Gaps = 1/78 (1%)
Frame = +1
Query: 1 GASAI-GQFVSADTKGGATGFGRAFNAGIGQESKEITLRKARIGITALCQQLRLNQQCID 177
GA+ + G VS D T G N G ES+E+T+ R I+AL L+LN++ I+
Sbjct: 54 GAAVVQGSLVSNDQTHARTFGGPYRNQG-SVESRELTIANGRRRISALAIALKLNERHIE 112
Query: 178 IACNFYKMALSRHLTIGR 231
A ++ +A++ + GR
Sbjct: 113 AAVRYFTLAINNNFIKGR 130
>SPAC16E8.16 |||transcription factor TFIIB |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 340
Score = 29.9 bits (64), Expect = 0.37
Identities = 12/41 (29%), Positives = 23/41 (56%)
Frame = +2
Query: 512 SGRRPSGICGAALLIAARLHEFSRTPSDIVRIVKVHESTLR 634
+GR P I + + + + L + +TP +I + V +ST+R
Sbjct: 267 AGRSPISIAASGIYMISALMGYPKTPKEISEVTGVSDSTIR 307
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/42 (26%), Positives = 20/42 (47%)
Frame = +3
Query: 249 SCCVYMTCRTEGTPHLLIDVSDAVPLCCYQLGRAYFKLSRAL 374
+ C+Y+ CR P +++ + ++GR Y L R L
Sbjct: 165 AACIYIACRQGKVPRTFMEICTLTNVPKKEIGRVYKTLQRML 206
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2
Query: 566 LHEFSRTPSDIVRIVKVHESTLRKTACSNSETPPFQGPEPSKS 694
L E + + V + TL T+ S PPFQ P PSK+
Sbjct: 400 LEEKNVDNQEFVTSISNGNQTLEDTSHSPQTQPPFQPPYPSKA 442
>SPCC162.12 ||SPCC1753.06c|sequence orphan|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 451
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 578 SRTPSDIVRIVKVHESTLRKTACSNSETPPFQGPEPSKSS 697
SR S + R+V VH++ + + S PPF + S+
Sbjct: 156 SRASSPVTRVVAVHDNKKKIINSNISNAPPFNNTDVQASA 195
>SPBC947.01 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +1
Query: 550 THSSSTARILTHPVRYRPYSQSS 618
+HSSS + HP Y+PY SS
Sbjct: 282 SHSSSDGKSRKHPSPYKPYLNSS 304
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 26.6 bits (56), Expect = 3.4
Identities = 21/90 (23%), Positives = 38/90 (42%)
Frame = +3
Query: 426 SQLKFEDKQHEVSMTALRLDQRMKKDSITRGEDPPEYVELHYS*QLDCTNSHAPRPISSV 605
+++KF S T L + K D RG+ E+V+L Q+D +SH ++
Sbjct: 651 NRVKFHSATQPFSQTTSLLFKEQKTDGSFRGQRQVEHVKLKMGMQID--DSHFRSMLAET 708
Query: 606 *SKFTSQHSAKRHVRIRRHPHSRALNPRRV 695
T + R + + L+P+R+
Sbjct: 709 NVLATKNYQKWRWDTLVQIMEGPLLSPKRI 738
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = -3
Query: 681 SGPWNGGVSEFEHAV 637
S W G VSEFEHAV
Sbjct: 118 SDHWEGKVSEFEHAV 132
>SPCC162.11c |||uridine kinase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 454
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -2
Query: 394 VGGMLMHRARDSLKYARPS**QHNG 320
+G MLM+ A DSL Y + + HNG
Sbjct: 284 IGTMLMNLAGDSLAYEKKTITLHNG 308
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,013,986
Number of Sequences: 5004
Number of extensions: 64720
Number of successful extensions: 157
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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