BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS31048 (609 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 141 8e-35 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 136 3e-33 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 66 5e-12 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 51 1e-07 SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.2 SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 2.8 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 27 2.8 SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 3.7 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.0 SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 5.0 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 6.5 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.7 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 8.7 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 141 bits (341), Expect = 8e-35 Identities = 67/124 (54%), Positives = 77/124 (62%) Frame = +2 Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 257 TYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLYSGKEDAANNYARGHYTIGKEIVDLVLD 436 P F L +GKEDA+NNYARGHYT+GKE+VD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 437 RIRK 448 +IR+ Sbjct: 121 KIRR 124 Score = 38.3 bits (85), Expect = 9e-04 Identities = 14/18 (77%), Positives = 17/18 (94%) Frame = +3 Query: 447 RLADQCTGLQGFLIFHSF 500 R+AD C+GLQGFL+FHSF Sbjct: 124 RIADNCSGLQGFLVFHSF 141 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 136 bits (328), Expect = 3e-33 Identities = 70/129 (54%), Positives = 80/129 (62%), Gaps = 5/129 (3%) Frame = +2 Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 241 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 242 TGAGSTYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLYSGKEDAANNYARGHYTIGKEIV 421 TG G P F + +GKEDA+NNYARGHYT+GKE++ Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119 Query: 422 DLVLDRIRK 448 D VL+RIR+ Sbjct: 120 DSVLERIRR 128 Score = 38.3 bits (85), Expect = 9e-04 Identities = 14/18 (77%), Positives = 17/18 (94%) Frame = +3 Query: 447 RLADQCTGLQGFLIFHSF 500 R+AD C+GLQGFL+FHSF Sbjct: 128 RMADNCSGLQGFLVFHSF 145 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 65.7 bits (153), Expect = 5e-12 Identities = 37/124 (29%), Positives = 53/124 (42%) Frame = +2 Query: 77 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 256 MRE + + GQ G Q+G A W EHG+ G T + N +F+E G Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 257 TYPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLYSGKEDAANNYARGHYTIGKEIVDLVLD 436 P + F +N+ G+ A N +A+GHYT G E+ D VLD Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118 Query: 437 RIRK 448 +R+ Sbjct: 119 VVRR 122 Score = 43.6 bits (98), Expect = 2e-05 Identities = 17/35 (48%), Positives = 26/35 (74%) Frame = +1 Query: 253 QHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLIF 357 ++VPRAV VDLEP +D V++G + LF P+ +I+ Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIY 92 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 51.2 bits (117), Expect = 1e-07 Identities = 37/123 (30%), Positives = 54/123 (43%), Gaps = 2/123 (1%) Frame = +2 Query: 80 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGST 259 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 260 YPVXXXXXXXXXXXXXXXXAHTDSCFIQNNLYSGKE--DAANNYARGHYTIGKEIVDLVL 433 P S + N+ K A NN+A G Y+ + I + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 434 DRI 442 D I Sbjct: 120 DMI 122 >SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 1024 Score = 27.9 bits (59), Expect = 1.2 Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%) Frame = -1 Query: 312 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 208 AD+IN SR+ ID S T + P+SL KE P Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 26.6 bits (56), Expect = 2.8 Identities = 15/41 (36%), Positives = 18/41 (43%) Frame = -2 Query: 515 PVLPPKGVEDQESLQTGTLVSESCGFCPKPNLRFPFQWCSD 393 PVLPP + D E ++ V E P PN P W D Sbjct: 235 PVLPPVEIYDPEDIKPADWVDEP--EIPDPNAVKPDDWDED 273 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = -2 Query: 524 PQKPVLPPKGVEDQESLQTGTLVSESCGFCPKP 426 P+KP LPP+ L + L SE+ P P Sbjct: 356 PEKPRLPPRPSHTLSELSSPALTSENLSSKPSP 388 >SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 26.2 bits (55), Expect = 3.7 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%) Frame = -1 Query: 345 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 178 F + + S +++N+ + KID GY S K + E + P + G+W Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 5.0 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +1 Query: 175 WPDAHRQDHRGWRRFFQHFLQR 240 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1067 Score = 25.8 bits (54), Expect = 5.0 Identities = 12/37 (32%), Positives = 23/37 (62%) Frame = -1 Query: 279 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 169 ++D T Y+ P P+ + L +++PP +V+ +G P G Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 6.5 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +3 Query: 66 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 188 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.0 bits (52), Expect = 8.7 Identities = 14/30 (46%), Positives = 17/30 (56%) Frame = +3 Query: 156 STASSLMARCPQTRPSGVETILSTLSSARP 245 ST SSL + ++PS T ST SSA P Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAP 202 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 25.0 bits (52), Expect = 8.7 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -3 Query: 130 ITDLDSGLANVYRDALTHFDLSF 62 +T +D GLA YRD TH + + Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,673,420 Number of Sequences: 5004 Number of extensions: 57423 Number of successful extensions: 181 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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