Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS31029
(874 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 158 1e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 155 1e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 5e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 7e-14
SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein Ucp7|Schizosaccha... 29 0.65
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 2.0
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 2.0
SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex ... 27 4.6
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 6.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 158 bits (383), Expect = 1e-39
Identities = 67/100 (67%), Positives = 82/100 (82%)
Frame = +2
Query: 221 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 400
F F ++VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 401 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 520
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGG G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 96.7 bits (230), Expect = 4e-21
Identities = 42/63 (66%), Positives = 46/63 (73%)
Frame = +3
Query: 78 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 257
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG G
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 258 TYP 266
P
Sbjct: 61 YVP 63
Score = 49.2 bits (112), Expect = 8e-07
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +1
Query: 508 SGSGFTSLLMERLSVDYGKEV*NWSSPSTPRPQVSTAVVEPYNFYPHQPTQTLEHSDCAF 687
+GSGF +LL+ERL+++Y K+ P PQVST+VVEPYN TL+ +DC F
Sbjct: 145 TGSGFGALLLERLAMEYTKKS-KLQFSVYPAPQVSTSVVEPYNSV-LTTHATLDLADCTF 202
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 155 bits (375), Expect = 1e-38
Identities = 64/100 (64%), Positives = 82/100 (82%)
Frame = +2
Query: 221 FQHFLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 400
F F + VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 401 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 520
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGG G
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSG 152
Score = 90.6 bits (215), Expect = 3e-19
Identities = 46/68 (67%), Positives = 48/68 (70%), Gaps = 5/68 (7%)
Frame = +3
Query: 78 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 242
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 243 TGAGSTYP 266
TG G P
Sbjct: 60 TGQGKFVP 67
Score = 55.2 bits (127), Expect = 1e-08
Identities = 30/60 (50%), Positives = 40/60 (66%)
Frame = +1
Query: 508 SGSGFTSLLMERLSVDYGKEV*NWSSPSTPRPQVSTAVVEPYNFYPHQPTQTLEHSDCAF 687
+GSG +LL+ERL+++YGK+ N P PQVST+VVEPYN TL++SDC F
Sbjct: 149 TGSGLGALLLERLNMEYGKKS-NLQFSVYPAPQVSTSVVEPYNSV-LTTHATLDNSDCTF 206
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 99.5 bits (237), Expect = 5e-22
Identities = 44/89 (49%), Positives = 60/89 (67%)
Frame = +2
Query: 254 QHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVL 433
++VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Query: 434 DRIRKLADQCTGLQGFLIFHSFGGGPALG 520
D +R+ A+ C LQGF + HS GGG G
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 38.7 bits (86), Expect = 0.001
Identities = 20/63 (31%), Positives = 27/63 (42%)
Frame = +3
Query: 78 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGS 257
MRE + + GQ G Q+G A W EHG+ G T + N +F+E G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 258 TYP 266
P
Sbjct: 59 YVP 61
Score = 30.7 bits (66), Expect = 0.28
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = +1
Query: 508 SGSGFTSLLMERLSVDYGKEV*NWSSPSTPRPQVSTAVVEPYN--FYPHQPTQTLEHSDC 681
+GSG +LL+ ++ +Y + S + P P+ S VVEPYN HQ + + + C
Sbjct: 143 TGSGMGTLLLSKIREEYPDRMMATFSVA-PAPKSSDTVVEPYNATLSMHQLVENSDETFC 201
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.5 bits (170), Expect = 7e-14
Identities = 37/99 (37%), Positives = 59/99 (59%), Gaps = 2/99 (2%)
Frame = +2
Query: 230 FLQRDRSWQHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 403
F Q D + +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 404 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGPALG 520
+ I + ++D I + AD L+GF + HS GG G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 48.8 bits (111), Expect = 1e-06
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +3
Query: 81 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 245
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
Score = 33.5 bits (73), Expect = 0.040
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +1
Query: 508 SGSGFTSLLMERLSVDYGKEV*NWSSPSTPRPQVSTAVVEPYN 636
+GSG S L+ERL+ Y K++ S VS VV+PYN
Sbjct: 145 TGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
>SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein
Ucp7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 697
Score = 29.5 bits (63), Expect = 0.65
Identities = 15/52 (28%), Positives = 22/52 (42%)
Frame = +3
Query: 507 VRLWVHFLIDGAXXXXXXXXXXKLEFAIYPAASGFHCRRRALQLLSSPTHTN 662
+ +W + DG + E AI +S H +R Q S+P HTN
Sbjct: 520 LNIWKDLIADGQISKLYIDGKHRCEAAISSHSSESHSKRTTQQPKSTPNHTN 571
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/36 (36%), Positives = 24/36 (66%)
Frame = -1
Query: 535 SIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 428
SIR ++SR+S+ G + Q S+Q+GT ++D ++
Sbjct: 108 SIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -1
Query: 313 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 209
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex
subunit Sld3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 668
Score = 26.6 bits (56), Expect = 4.6
Identities = 24/94 (25%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +1
Query: 589 STPRPQVSTAVVEPYNFYPHQPTQTLEHSDCAFHGSTMKPSMTSGRRNLR-HLSGPNLTQ 765
S+ P+++ ++E N P ++ +H A S + S + R L + SG +++
Sbjct: 406 SSKFPRITRNLLEKCNGLDLLPERSHKHRHSAPPRSKLISSKSEAGRALPGNTSGASISN 465
Query: 766 TESSQSGQDLSPPIKWVPLRISNGGPEIWAFTRS 867
T S S +S + + R SN G + + TRS
Sbjct: 466 TSSPHSEASISKDYEILKRRRSNSG--VHSLTRS 497
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 6.1
Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = -1
Query: 346 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 179
F + + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 176 WPDAHRQDHRGWRRFFQHFLQR 241
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -1
Query: 280 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 170
++D T Y+ P P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,924,652
Number of Sequences: 5004
Number of extensions: 88604
Number of successful extensions: 266
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 262
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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