Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS31027
(868 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 157 3e-39
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 98 2e-21
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 97 2e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 72 1e-13
SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces pom... 31 0.21
SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces p... 29 0.65
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 29 1.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.6
SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosacch... 27 2.6
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.5
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 3.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.6
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 6.0
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 8.0
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 26 8.0
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 157 bits (380), Expect = 3e-39
Identities = 75/128 (58%), Positives = 88/128 (68%)
Frame = +1
Query: 256 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 435
DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+
Sbjct: 67 DLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEA 126
Query: 436 CDCLQGFQXXXXXXXXXXXXXXXXPSQRSVKSTPTES*THTQ*SPRPKVSDTVVEPYNAT 615
CD LQGFQ + + P +P PK SDTVVEPYNAT
Sbjct: 127 CDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNAT 186
Query: 616 LSVHQLVE 639
LS+HQLVE
Sbjct: 187 LSMHQLVE 194
Score = 116 bits (279), Expect = 4e-27
Identities = 48/70 (68%), Positives = 57/70 (81%)
Frame = +2
Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 238
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 239 PRAILATWSP 268
PRA+L P
Sbjct: 61 PRAVLVDLEP 70
Score = 35.5 bits (78), Expect = 0.010
Identities = 29/87 (33%), Positives = 45/87 (51%), Gaps = 1/87 (1%)
Frame = +2
Query: 599 NHTMRLSQFTS*LKNTDENYCIDNEGSIRHLLPQLLKLFHNPPYGDL*PLSFPSHVLVS- 775
N T+ + Q ++N+DE +CIDNE ++ + LK+ +P Y DL L S V+
Sbjct: 184 NATLSMHQL---VENSDETFCIDNE-ALSSIFANTLKI-KSPSYDDLNHL--VSAVMAGV 236
Query: 776 TLALRFPGSVERLIFKKNWP*TWVPFP 856
T + RFPG + + + VPFP
Sbjct: 237 TTSFRFPGELNSDL--RKLAVNMVPFP 261
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 97.9 bits (233), Expect = 2e-21
Identities = 47/128 (36%), Positives = 69/128 (53%)
Frame = +1
Query: 256 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 435
DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 436 CDCLQGFQXXXXXXXXXXXXXXXXPSQRSVKSTPTES*THTQ*SPRPKVSDTVVEPYNAT 615
C LQGF +R +S P P+VS +VVEPYN+
Sbjct: 129 CSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188
Query: 616 LSVHQLVE 639
L+ H ++
Sbjct: 189 LTTHATLD 196
Score = 57.6 bits (133), Expect = 2e-09
Identities = 28/72 (38%), Positives = 39/72 (54%), Gaps = 2/72 (2%)
Frame = +2
Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 232
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 233 YVPRAILATWSP 268
YVPR+I P
Sbjct: 61 YVPRSIYVDLEP 72
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 97.5 bits (232), Expect = 2e-21
Identities = 47/128 (36%), Positives = 71/128 (55%)
Frame = +1
Query: 256 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 435
DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 436 CDCLQGFQXXXXXXXXXXXXXXXXPSQRSVKSTPTES*THTQ*SPRPKVSDTVVEPYNAT 615
C LQGF +R +S P P+VS +VVEPYN+
Sbjct: 133 CSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192
Query: 616 LSVHQLVE 639
L+ H ++
Sbjct: 193 LTTHATLD 200
Score = 51.6 bits (118), Expect = 1e-07
Identities = 26/76 (34%), Positives = 38/76 (50%), Gaps = 6/76 (7%)
Frame = +2
Query: 59 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 220
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 221 SGGKYVPRAILATWSP 268
GK+VPR+I P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 71.7 bits (168), Expect = 1e-13
Identities = 31/69 (44%), Positives = 46/69 (66%)
Frame = +2
Query: 62 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 241
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 242 RAILATWSP 268
RAIL P
Sbjct: 63 RAILIDLEP 71
Score = 66.1 bits (154), Expect = 6e-12
Identities = 36/129 (27%), Positives = 67/129 (51%), Gaps = 3/129 (2%)
Frame = +1
Query: 256 DLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKES 429
DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E+
Sbjct: 68 DLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREA 126
Query: 430 ESCDCLQGFQXXXXXXXXXXXXXXXXPSQRSVKSTPTE-S*THTQ*SPRPKVSDTVVEPY 606
+ D L+GF +R P + T++ VSD VV+PY
Sbjct: 127 DGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPY 186
Query: 607 NATLSVHQL 633
N+ L++ +L
Sbjct: 187 NSLLALKRL 195
>SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 202
Score = 31.1 bits (67), Expect = 0.21
Identities = 20/56 (35%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +2
Query: 650 ENYCIDNEGSIRHLLPQLLKLFHNPPYGDL*PLSFP-SHVLVSTLALRFPGSVERL 814
ENY D E RH+ L Y L PLS+P SHV++ A+ P S++ +
Sbjct: 41 ENYVADVEVDGRHVELALWDTAGQEDYDRLRPLSYPDSHVILICFAVDSPDSLDNV 96
>SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 200
Score = 29.5 bits (63), Expect = 0.65
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +2
Query: 650 ENYCIDNEGSIRHLLPQLLKLFHNPPYGDL*PLSFP-SHVLVSTLALRFPGSVERL 814
ENY D E RH+ L Y L PLS+P SHV++ ++ P S++ +
Sbjct: 41 ENYVADVEVDGRHIELALWDTAGQEDYDRLRPLSYPDSHVVLICFSVDAPESLDNV 96
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +1
Query: 226 RQVRAPRHPRDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 354
R+V AP+H R GT RS +FR ++ FGQ GN
Sbjct: 486 REVFAPKHARR-RLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 361 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 450
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase
Nak1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 652
Score = 27.5 bits (58), Expect = 2.6
Identities = 14/50 (28%), Positives = 26/50 (52%)
Frame = +2
Query: 560 SSPLAQKYQTLSSNHTMRLSQFTS*LKNTDENYCIDNEGSIRHLLPQLLK 709
SS L+ K T+ S HT + + + T N ++ SI+ LP++++
Sbjct: 418 SSNLSSKKSTIQSKHTKQAEDYDLFVGRTRSNSKTSSDQSIKRPLPRVVQ 467
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 98 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 199
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 27.1 bits (57), Expect = 3.5
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 458 NLHIPSVAAPGPVWAPSHLKDP*RVPRQNHEHILSSPLAQ 577
N+H PS A + +++P +P+QNHEH SPL +
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVR 659
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.6
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 292 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 393
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 6.0
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3
Query: 42 KITYTK*GKSFIYRPANVV 98
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 25.8 bits (54), Expect = 8.0
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 3/35 (8%)
Frame = +3
Query: 492 QYGHPPISKIREEYP-DRIMNTYSVVP--SPKSIR 587
QY H SK++ EYP D+++ SV+ PK I+
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQ 426
>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1159
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/48 (27%), Positives = 28/48 (58%)
Frame = +2
Query: 530 VPRQNHEHILSSPLAQKYQTLSSNHTMRLSQFTS*LKNTDENYCIDNE 673
+ +Q+ H LS+P ++K QTL H + F++ ++ DE + +++
Sbjct: 313 IDKQDIYHRLSTPTSRKRQTLEKGH---IKAFSAVDEDLDEIFACEDD 357
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,545,334
Number of Sequences: 5004
Number of extensions: 72454
Number of successful extensions: 219
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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