Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS31003
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 46 5e-06
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 33 0.039
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 33 0.069
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.5
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 28 2.0
SPBC337.06c |cwf15||complexed with Cdc5 protein Cwf15 |Schizosac... 27 3.4
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 26 6.0
SPAC3G9.05 |||GTPase activating protein |Schizosaccharomyces pom... 26 6.0
>SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 46.4 bits (105), Expect = 5e-06
Identities = 27/66 (40%), Positives = 36/66 (54%)
Frame = +2
Query: 311 YVYKLCMFQKVTQKSKNGGMEVGLGNWGEWAGPENNKYSVMKYTNGIACWNGPSRTTTVN 490
Y YK+ ++ V Q S + LGN+ A E N V+KY NG +CWNGP R+ V
Sbjct: 395 YTYKVVFYENVFQDS------ILLGNF---ASQEGN---VLKYENGQSCWNGPHRSAIVT 442
Query: 491 VNCDLE 508
V C +E
Sbjct: 443 VECGVE 448
Score = 30.7 bits (66), Expect = 0.28
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 511 KITSVTEPFRCEYKMELSTPAACDDSSTSQ 600
+I SV E +CEY +++ +PAAC Q
Sbjct: 450 EIVSVLEAQKCEYLIKMKSPAACSPDQLKQ 479
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 33.5 bits (73), Expect = 0.039
Identities = 20/58 (34%), Positives = 28/58 (48%)
Frame = -3
Query: 588 RIITCSRSA*FHFVFTTERFSY*CDLCSKSQLTLTVVVRLGPFQQAIPFVYFITEYLL 415
+I+ CS +H +F +F Y C + SQ+ L + LGP Q A FV Y L
Sbjct: 1041 QIVACSSLGVYHSIFNKRKFDY-CIIDEASQIPLPIC--LGPLQLAEKFVLVGDHYQL 1095
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 32.7 bits (71), Expect = 0.069
Identities = 16/49 (32%), Positives = 28/49 (57%)
Frame = +3
Query: 99 VSEQSKSYNDETQKIVDQASEARRQYTDAERTVREIESNIRNIKQNLEK 245
+S+ SK +D + + +EA R+ E+T+RE E +I K+ +EK
Sbjct: 555 LSKSSKESDDYEEVVGKLRTEAEREIEKFEKTIRENEESISLFKEEVEK 603
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 353 IFVLPSGTYTVCIHILCLHIQSNHHPR 273
IFV P + + C+ +CL + SN +PR
Sbjct: 173 IFVPPKRSSSSCVTYVCLFLDSNSNPR 199
>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +3
Query: 3 EENHEELDADFEG--NEEDNSXXXXXXXXXXXXTVSEQSKSYNDETQKIVDQAS 158
EEN ++ DA EED+ + SE+ +S NDE +++DQ +
Sbjct: 353 EENEKKYDAIKHEILGEEDDDENEEDEEDSEETSESEEDESVNDEKPQVIDQTN 406
>SPBC337.06c |cwf15||complexed with Cdc5 protein Cwf15
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 265
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/79 (22%), Positives = 40/79 (50%), Gaps = 1/79 (1%)
Frame = +3
Query: 15 EELDADFEGNEEDNSXXXXXXXXXXXXTVSEQSKSYNDETQKIVDQA-SEARRQYTDAER 191
+ L+AD + ++ D+S VS + + +Q+ VD + SE+ + +D+E
Sbjct: 110 QTLEADEDASDSDDSVDSSNKNSE----VSIKRRKTESNSQESVDSSNSESSDEESDSED 165
Query: 192 TVREIESNIRNIKQNLEKD 248
+++ + NIKQ +++
Sbjct: 166 ETQQLLRELENIKQERKRE 184
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 26.2 bits (55), Expect = 6.0
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 417 LFSGPAHSPQLPRPTSIPPFLDFCV 343
L S +LP+P S+PP L+ C+
Sbjct: 216 LTSNTLQELKLPKPPSLPPHLEKCI 240
>SPAC3G9.05 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 659
Score = 26.2 bits (55), Expect = 6.0
Identities = 21/80 (26%), Positives = 44/80 (55%), Gaps = 7/80 (8%)
Frame = +3
Query: 96 TVSEQSKSYNDETQ---KIVDQASEAR---RQYTDAERTVREIESN-IRNIKQNLEKDYG 254
++S+ +K N+ Q K+ + + EA ++ DAE ++E+E+N + LEK
Sbjct: 258 SISDATKHKNEIFQLEEKLHEASHEAEISIKKLNDAENRIKELENNPTLSFNPELEK--- 314
Query: 255 *NRNLLPWMVIALNMKTKNM 314
N L+ +++A + KT+++
Sbjct: 315 -NLKLMQELIVAESSKTQHI 333
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,391,842
Number of Sequences: 5004
Number of extensions: 75212
Number of successful extensions: 192
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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