Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30991
         (703 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   103   2e-23
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    70   4e-13
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    69   7e-13
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    50   3e-07
SPAC17A5.05c |||conserved fungal protein|Schizosaccharomyces pom...    32   0.069
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    28   1.5  
SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc...    27   3.4  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.4  
SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1 |Schizosa...    26   6.0  
SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch...    26   6.0  
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   7.9  
SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb...    25   7.9  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   7.9  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  103 bits (248), Expect = 2e-23
 Identities = 57/125 (45%), Positives = 73/125 (58%)
 Frame = +3

Query: 255 AILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV*TSFER 434
           A+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+V     R
Sbjct: 63  AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122

Query: 435 KRNRVNASRDSN*HTRSXXXXXXXXXXSFIFKIHLKSTLTELMNTFSSCYPSPKSCRTTI 614
           +    +A +     T S          + +     +     +M TFS   P+PKS  T +
Sbjct: 123 EAEACDALQGFQ-LTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVA-PAPKSSDTVV 180

Query: 615 SLNPY 629
              PY
Sbjct: 181 --EPY 183



 Score = 73.7 bits (173), Expect = 2e-14
 Identities = 30/43 (69%), Positives = 34/43 (79%)
 Frame = +2

Query: 71  MREIVHIQAGQCGNQIGAKSWEIISDEHGIDPTGAYHGDSDLQ 199
           MREIVHIQAGQCGNQ+GA  W  I+DEHG+D  G YHG S+ Q
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQ 43



 Score = 34.7 bits (76), Expect = 0.013
 Identities = 13/16 (81%), Positives = 16/16 (100%)
 Frame = +1

Query: 202 ERINVYYNEASGGKYV 249
           ER+NVY+NEA+GGKYV
Sbjct: 45  ERLNVYFNEAAGGKYV 60


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 28/54 (51%), Positives = 39/54 (72%)
 Frame = +3

Query: 255 AILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV 416
           +I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSV
Sbjct: 69  SIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSV 122



 Score = 41.9 bits (94), Expect = 9e-05
 Identities = 17/34 (50%), Positives = 21/34 (61%)
 Frame = +2

Query: 71  MREIVHIQAGQCGNQIGAKSWEIISDEHGIDPTG 172
           MRE++ +  GQ G QIG   WE+   EHGI P G
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG 34


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 28/54 (51%), Positives = 38/54 (70%)
 Frame = +3

Query: 255 AILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV 416
           +I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V
Sbjct: 65  SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEV 118



 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 22/51 (43%), Positives = 29/51 (56%)
 Frame = +2

Query: 71  MREIVHIQAGQCGNQIGAKSWEIISDEHGIDPTGAYHGDSDLQWSASMYTT 223
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q S   ++T
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFST 51


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 20/33 (60%), Positives = 26/33 (78%)
 Frame = +2

Query: 74  REIVHIQAGQCGNQIGAKSWEIISDEHGIDPTG 172
           REI+ +QAGQCGNQIG++ W+ +  EHGI P G
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35



 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 21/63 (33%), Positives = 39/63 (61%), Gaps = 2/63 (3%)
 Frame = +3

Query: 255 AILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSV*TSF 428
           AIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + +    
Sbjct: 64  AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122

Query: 429 ERK 437
           +R+
Sbjct: 123 DRE 125


>SPAC17A5.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 32.3 bits (70), Expect = 0.069
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = +3

Query: 525 FKIHLKSTLTELMNTFSSCYPSPKSCRTTISLNPYKGPQ 641
           F +HL   LTE    FS+CY     CR  + L P+K P+
Sbjct: 168 FALHL---LTEPSKLFSTCYALSVQCRWLLVLGPHKKPE 203


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +2

Query: 110 NQIGAKSWEIISDEHGIDPTGAYHGDSDLQWSASMYTT 223
           N++G    E+++++  +DPT A   + DLQ  A +  T
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAVLKPT 170


>SPCC10H11.01 |prp11||ATP-dependent RNA helicase
           Prp11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1014

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = -3

Query: 353 GLSEDEVVRTEDLSERSRANRVHGAGLQVDEDG 255
           G+S  E+ R +D S+  R NRVH      DEDG
Sbjct: 240 GISGFEINRQKDTSDMKRNNRVH----MDDEDG 268


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 303 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 404
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 652

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
 Frame = +1

Query: 67  QNEGNRTYPSWSMRQPDR--S*VLGDHLRRARHRPHRCLPWGL*LAVERINVYYNEASGG 240
           + EG+    SW+  QP +    +  D+ + A+ +  R   WG     ++I   ++  SG 
Sbjct: 71  EREGSSLTHSWTF-QPGKHNQRLYSDNFQEAQRQWKRLQEWGEVKETKKIRKRFDRFSGR 129

Query: 241 KYV 249
           KY+
Sbjct: 130 KYI 132


>SPBC3F6.01c |||serine/threonine protein phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 13/54 (24%), Positives = 28/54 (51%)
 Frame = +2

Query: 35  VLSNYSSHK*NKMREIVHIQAGQCGNQIGAKSWEIISDEHGIDPTGAYHGDSDL 196
           V  N  +H+ + M ++   + G+C ++   +++ I S+   + P G+   DS L
Sbjct: 272 VFINRGNHETDDMNKVYGFE-GECRSKYNERTFNIFSETFSLLPLGSLISDSYL 324


>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 230

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 20/59 (33%), Positives = 28/59 (47%), Gaps = 3/59 (5%)
 Frame = +3

Query: 531 IHLKSTLTELMNTFSSCY---PSPKSCRTTISLNPYKGPQFFQFPSN*FEKHPDGKPNF 698
           I+  S L    N   SC+     P +  T++S+NP K    F FP +  +K P   PNF
Sbjct: 47  INTHSNLCLFSNFSHSCFLFCTHPDTLPTSLSINPKKLSLSFSFPLS--QKRP--FPNF 101


>SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 224

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/41 (31%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
 Frame = +3

Query: 573 SSCYPSPK-SCRTTISLNPYKGPQFFQFPSN*FEKHPDGKP 692
           S+ Y SP  S     +  PY+  QF+  P + +   P G+P
Sbjct: 91  STMYASPPPSSNYNTAKPPYQTSQFYARPQSSYAPPPSGRP 131


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -1

Query: 361 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 266
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,780,216
Number of Sequences: 5004
Number of extensions: 54689
Number of successful extensions: 156
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)