Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30981
(721 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 75 1e-14
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 3e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 46 7e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 37 0.003
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.004
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.031
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 33 0.054
SPAPJ760.03c |adg1||sequence orphan|Schizosaccharomyces pombe|ch... 28 1.2
SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces p... 28 1.5
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 27 2.0
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 3.6
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 3.6
SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase |Schizos... 26 6.2
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 26 6.2
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 25 8.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 8.2
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 74.9 bits (176), Expect = 1e-14
Identities = 36/81 (44%), Positives = 54/81 (66%), Gaps = 1/81 (1%)
Frame = +3
Query: 258 KVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSSKELL 437
+VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+ K +
Sbjct: 77 EVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVKP-I 134
Query: 438 TVADFEAFTSK-DEVVVVGFF 497
+ E F K D++ VV FF
Sbjct: 135 SKDTLENFVEKADDLAVVAFF 155
Score = 50.8 bits (116), Expect = 2e-07
Identities = 23/57 (40%), Positives = 32/57 (56%)
Frame = +1
Query: 67 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTD 237
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA L+ D
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD 71
Score = 48.4 bits (110), Expect = 1e-06
Identities = 25/61 (40%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +1
Query: 82 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALL 258
++ED++ L +F V+ + LV FYAPWCGHCK L P Y A +DD V +
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY-SDDSNVVVA 411
Query: 259 K 261
K
Sbjct: 412 K 412
Score = 26.6 bits (56), Expect = 3.6
Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = +3
Query: 255 AKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM--RAQVGPS 422
AK+D TE S S+SG+PT+ F+ + + Y G R + ++ A P
Sbjct: 411 AKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEPI 466
Query: 423 SKE 431
KE
Sbjct: 467 KKE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 50.0 bits (114), Expect = 3e-07
Identities = 26/84 (30%), Positives = 42/84 (50%)
Frame = +1
Query: 4 KAPAKFEVFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 183
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 184 CKRLKPEYAVAAGLLKTDDPPVAL 255
CK+L P Y A L + P A+
Sbjct: 63 CKKLVPTYQKLASNLHSLLPVTAV 86
Score = 39.5 bits (88), Expect = 5e-04
Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
Frame = +3
Query: 261 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGPRESNGIVKYMRAQVGPS 422
VDC + ++ C Q+ V G+PT+K+ KG S++YNG R + K++ + PS
Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PS 144
Query: 423 SKELLT 440
++LT
Sbjct: 145 KVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 45.6 bits (103), Expect = 7e-06
Identities = 22/58 (37%), Positives = 33/58 (56%), Gaps = 1/58 (1%)
Frame = +1
Query: 91 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALLK 261
+V++L +F V+ LV FYA WCG+CKRL P Y + K ++P V ++K
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFK-NEPNVEIVK 197
Score = 39.5 bits (88), Expect = 5e-04
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1
Query: 148 ALVMFYAPWCGHCKRLKPEYAVAAGLLK 231
AL+ FYA WCGHCK L P Y L +
Sbjct: 42 ALIEFYATWCGHCKSLAPVYEELGALFE 69
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 36.7 bits (81), Expect = 0.003
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +1
Query: 112 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 207
S+F +++ Q +V F+A WCG CK + P++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.3 bits (80), Expect = 0.004
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +1
Query: 115 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 201
D++ +S +V FYA WCG CK LKP
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP 55
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 33.5 bits (73), Expect = 0.031
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1
Query: 103 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 201
LTD+D + +S+ T + +Y P CG CKRL P
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +3
Query: 255 AKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 398
A ++C K C+Q+S+ +PT +F K E EY G +V +
Sbjct: 334 AHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 32.7 bits (71), Expect = 0.054
Identities = 28/105 (26%), Positives = 46/105 (43%), Gaps = 2/105 (1%)
Frame = -1
Query: 505 SFSKNPTTTTSSLEVKASKSATVRSSLELGPTWARMYLTMPLDSLGPLYSEESSPFLNIF 326
S + P +TS E K + EL + R T +D P+ ++S +
Sbjct: 568 SVGEAPVESTSKEEDKDVPDPIANAMAELSSSMRRRQSTS-VDDEAPVSLSKTSSSTRLN 626
Query: 325 SVGYP--DTENCSQVLLPPSVQSTLAAPPEGHRSLKGRRLPHTLA 197
+GY +T S + P +STL APP H S + +R+ ++ A
Sbjct: 627 GLGYHSRNTSIASDIDGVPK-KSTLGAPPAAHTSAQMQRMSNSFA 670
>SPAPJ760.03c |adg1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 166
Score = 28.3 bits (60), Expect = 1.2
Identities = 19/74 (25%), Positives = 35/74 (47%)
Frame = -1
Query: 532 FQELSFQVDSFSKNPTTTTSSLEVKASKSATVRSSLELGPTWARMYLTMPLDSLGPLYSE 353
+ + S ++DS S +PT+ T++ V ++ +V S+ PT P+D+ PL +
Sbjct: 78 YAKRSIEIDSDSVSPTSATTTTPVASATDVSVYSASIHVPTG-----NPPVDTHNPLSYD 132
Query: 352 ESSPFLNIFSVGYP 311
FS+ P
Sbjct: 133 TEVTATTTFSIALP 146
>SPAC23D3.03c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 472
Score = 27.9 bits (59), Expect = 1.5
Identities = 30/106 (28%), Positives = 48/106 (45%), Gaps = 1/106 (0%)
Frame = +1
Query: 100 DLTDSDF-SAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALLK*TVRK 276
+L D F AV +Q ++L FY + K+ +PE A L+K + P + +RK
Sbjct: 338 NLLDKPFLQAVYTQDTSSLKSFYQTFLDTLKKNEPELATHL-LIKLELVPDDFVYPLLRK 396
Query: 277 VARVLVNNSLCPDILH*KYSEKESFLQNTMDQGSLMALSSTCVPKL 414
+ +V+ + IL E++SF LMA+ PKL
Sbjct: 397 LFIPMVSPEIASRILDCYVFEEDSFFIQL-----LMAVFKLLKPKL 437
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 27.5 bits (58), Expect = 2.0
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +1
Query: 163 YAPWCGHCKRLKPEYAVAA 219
YA WCG CK + P ++ A
Sbjct: 27 YADWCGPCKAISPLFSQLA 45
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 3.6
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +2
Query: 95 FSILQIPTFRLFYLNMIQPWSCF 163
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2
Query: 614 NVVLYRPKRLQNKFLRLIWLAF 679
N+VLY PKR QN L+ + L F
Sbjct: 1328 NIVLYAPKRFQNAPLKQMALNF 1349
>SPAC6F6.11c |||pyridoxine-pyridoxal-pyridoxamine kinase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 6.2
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 539 DKLREEVTFAHSSANEVLEKT 601
DKL++ V A SS +EV++KT
Sbjct: 248 DKLKKSVEMALSSVHEVIQKT 268
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 25.8 bits (54), Expect = 6.2
Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
Frame = +1
Query: 85 EEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKT--DDPPVALL 258
EE V+D+ + D +AVL L + +P+ CK P+ V A +L+T + +L+
Sbjct: 324 EEAVIDVDELDKNAVLFGGTRVLQVTQSPF---CKLKTPDNGVPAIVLRTGFETSQGSLV 380
Query: 259 K*TVRKVARVLVNN 300
+ V +V NN
Sbjct: 381 RTMVFSSEKVTANN 394
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.4 bits (53), Expect = 8.2
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +1
Query: 151 LVMFYAPWCGHCKRL 195
L+ FYAPW CK++
Sbjct: 24 LLNFYAPWAAPCKQM 38
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/50 (26%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
Frame = -1
Query: 556 LLP*FVSCFQELSFQVDSFSKNPTTTTSSLEVKAS---KSATVRSSLELG 416
LLP F+ CF +++ + S P+ + +L++ + + + ++S +ELG
Sbjct: 2125 LLPGFMRCFHKVAKEFLSLGSQPSGNSLNLQIVNAVDERVSILKSMIELG 2174
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,079,827
Number of Sequences: 5004
Number of extensions: 64525
Number of successful extensions: 204
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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