Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30951
(879 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04620.2 68418.m00464 aminotransferase class I and II family ... 57 2e-08
At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative... 48 1e-05
At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2) id... 47 1e-05
At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative... 43 2e-04
At5g04620.1 68418.m00465 aminotransferase class I and II family ... 33 0.19
At4g36480.1 68417.m05180 aminotransferase class I and II family ... 31 1.3
At5g01650.1 68418.m00081 macrophage migration inhibitory factor ... 29 3.1
At5g41880.1 68418.m05099 DNA primase small subunit family contai... 28 9.5
At1g54100.2 68414.m06167 aldehyde dehydrogenase, putative / anti... 28 9.5
At1g54100.1 68414.m06166 aldehyde dehydrogenase, putative / anti... 28 9.5
>At5g04620.2 68418.m00464 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 476
Score = 56.8 bits (131), Expect = 2e-08
Identities = 25/60 (41%), Positives = 37/60 (61%)
Frame = +2
Query: 329 RRVTVWCANDYLGTSRHPTVQDAAVNAIKSYGTGAGGTRNIAGNSQMTEKLEGEIAKLHK 508
+++ ++ NDYLG S HPT+ +AA NA+K YG G G+ I G + LE +A+L K
Sbjct: 102 KKLLLFSGNDYLGLSSHPTISNAAANAVKEYGMGPKGSALICGYTTYHRLLESSLAQLKK 161
Score = 33.5 bits (73), Expect = 0.19
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 4/41 (9%)
Frame = +1
Query: 595 VYSDAGNHASMIQGI----RNSRAPKHIFRHNDPNHYGSLL 705
++SDA NHAS+I G+ R ++RH D H SLL
Sbjct: 203 IFSDALNHASIIDGVRLAERQGNVEVFVYRHCDMYHLNSLL 243
>At3g48780.1 68416.m05327 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 489
Score = 47.6 bits (108), Expect = 1e-05
Identities = 24/66 (36%), Positives = 36/66 (54%)
Frame = +1
Query: 508 EPAALIFSSCFVANDATLSTLAKILPDCIVYSDAGNHASMIQGIRNSRAPKHIFRHNDPN 687
+PAA+IF + N A + L I ++ SD+ NH S++ G R S A +F+HN P
Sbjct: 161 QPAAVIFGMGYATNSAIIPVL--IGKGGLIISDSLNHTSIVNGARGSGATIRVFQHNTPG 218
Query: 688 HYGSLL 705
H +L
Sbjct: 219 HLEKVL 224
>At5g23670.1 68418.m02775 serine C-palmitoyltransferase (LCB2)
identical to serine palmitoyltransferase [Arabidopsis
thaliana] GI:9309380; similar to serine
palmitoyltransferase from Solanum tuberosum
[GI:4995890], Homo sapiens [SP|O15270], Mus musculus
[SP|P97363]; contains Pfam profile PF00155:
aminotransferase, classes I and II
Length = 489
Score = 47.2 bits (107), Expect = 1e-05
Identities = 23/66 (34%), Positives = 38/66 (57%)
Frame = +1
Query: 508 EPAALIFSSCFVANDATLSTLAKILPDCIVYSDAGNHASMIQGIRNSRAPKHIFRHNDPN 687
+PAA++F + N A + L I ++ SD+ NH+S++ G R S A +F+HN P+
Sbjct: 161 KPAAVVFGMGYATNSAIIPVL--IGKGGLIISDSLNHSSIVNGARGSGATIRVFQHNTPS 218
Query: 688 HYGSLL 705
H +L
Sbjct: 219 HLERVL 224
>At3g48790.1 68416.m05328 serine C-palmitoyltransferase, putative
similar to serine palmitoyltransferase from Solanum
tuberosum [GI:4995890], Homo sapiens [SP|O15270], Mus
musculus [SP|P97363]
Length = 350
Score = 43.2 bits (97), Expect = 2e-04
Identities = 22/57 (38%), Positives = 34/57 (59%)
Frame = +1
Query: 508 EPAALIFSSCFVANDATLSTLAKILPDCIVYSDAGNHASMIQGIRNSRAPKHIFRHN 678
+PAA++F ++ N A +S L I ++ SD+ NH S+I G R S A +F+HN
Sbjct: 25 KPAAVVFGMGYLTNSAIISVL--IGKGGLIISDSLNHTSIINGARGSGATIRVFQHN 79
>At5g04620.1 68418.m00465 aminotransferase class I and II family
protein similar to 8-amino-7-oxononanoate synthase,
Bacillus sphaericus, PIR:JQ0512 [SP|P22806], Bacillus
subtilis [SP|P53556]; contains Pfam protile PF00155
aminotransferase, classes I and II
Length = 343
Score = 33.5 bits (73), Expect = 0.19
Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 4/41 (9%)
Frame = +1
Query: 595 VYSDAGNHASMIQGI----RNSRAPKHIFRHNDPNHYGSLL 705
++SDA NHAS+I G+ R ++RH D H SLL
Sbjct: 70 IFSDALNHASIIDGVRLAERQGNVEVFVYRHCDMYHLNSLL 110
>At4g36480.1 68417.m05180 aminotransferase class I and II family
protein similar to Serine palmitoyltransferase 1 (EC
2.3.1.50) from Homo sapiens [SP|O15269], Mus musculus
[SP|O35704], Cricetulus griseus [SP|O54695]
Length = 482
Score = 30.7 bits (66), Expect = 1.3
Identities = 15/55 (27%), Positives = 27/55 (49%)
Frame = +2
Query: 335 VTVWCANDYLGTSRHPTVQDAAVNAIKSYGTGAGGTRNIAGNSQMTEKLEGEIAK 499
V + + +YLG H + ++ +A++ YG G+ G R G + E I+K
Sbjct: 111 VVNFASANYLGLIGHEKLLESCTSALEKYGVGSCGPRGFYGTIDVHLDCETRISK 165
>At5g01650.1 68418.m00081 macrophage migration inhibitory factor
family protein / MIF family protein contains pfam
profile: PF001187 Macrophage migration inhibitory factor
Length = 115
Score = 29.5 bits (63), Expect = 3.1
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = -3
Query: 448 IPCTTCSCSIGFDSVDSSILHSWVTGRP*VVIGTPDSYSTI 326
+PC S ++ D VD+S + S + +IG P++Y I
Sbjct: 1 MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMI 41
>At5g41880.1 68418.m05099 DNA primase small subunit family contains
Pfam profile: PF01896 DNA primase small subunit
Length = 407
Score = 27.9 bits (59), Expect = 9.5
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = +2
Query: 299 YPKALEGPENRRVTVWCANDYLGTSRHPTVQDAAVNAIKSYGTG-AGGTRNIAGNSQMTE 475
Y + +G EN V D +G S HP + + V+ IKS+ G T++I + + E
Sbjct: 198 YFRVYKGNENNAKKV----DLMGHSLHPFLARSYVDFIKSFFEGELQATQSIFSSKEKYE 253
Query: 476 KLEGEI 493
K+ G I
Sbjct: 254 KILGMI 259
>At1g54100.2 68414.m06167 aldehyde dehydrogenase, putative /
antiquitin, putative strong similarity to SP|Q41247
Aldehyde dehydrogenase family 7 member A1 (EC 1.2.1.3)
(Antiquitin 1) (Brassica
turgor-responsive/drought-induced gene 26 protein)
(Btg-26) {Brassica napus}; similar to turgor-responsive
protein 26G (aldehyde dehydrogenase family 7 member A1)
[Pisum sativum] SWISS-PROT:P25795
Length = 508
Score = 27.9 bits (59), Expect = 9.5
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = -3
Query: 442 CTTCSCSIGFDSVDSSILHSWVTGRP*VVIGTPDSYSTIFRPL 314
CTTC + +SV +L +T V IG P T+ PL
Sbjct: 300 CTTCRRLLLHESVYDKVLEQLLTSYKQVKIGNPLEKGTLLGPL 342
>At1g54100.1 68414.m06166 aldehyde dehydrogenase, putative /
antiquitin, putative strong similarity to SP|Q41247
Aldehyde dehydrogenase family 7 member A1 (EC 1.2.1.3)
(Antiquitin 1) (Brassica
turgor-responsive/drought-induced gene 26 protein)
(Btg-26) {Brassica napus}; similar to turgor-responsive
protein 26G (aldehyde dehydrogenase family 7 member A1)
[Pisum sativum] SWISS-PROT:P25795
Length = 508
Score = 27.9 bits (59), Expect = 9.5
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = -3
Query: 442 CTTCSCSIGFDSVDSSILHSWVTGRP*VVIGTPDSYSTIFRPL 314
CTTC + +SV +L +T V IG P T+ PL
Sbjct: 300 CTTCRRLLLHESVYDKVLEQLLTSYKQVKIGNPLEKGTLLGPL 342
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,253,818
Number of Sequences: 28952
Number of extensions: 442062
Number of successful extensions: 1076
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1049
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1076
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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