Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30950
(779 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 50 5e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 35 0.011
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.11
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 4.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 26 5.3
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 7.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +1
Query: 4 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTA 183
A +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTA
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTA 340
Query: 184 QVIVLNHPGQISNGYTPVLDCHTA 255
QVI+LNHPGQIS GY+PVLDCHTA
Sbjct: 341 QVIILNHPGQISAGYSPVLDCHTA 364
Score = 126 bits (304), Expect = 4e-30
Identities = 58/87 (66%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAV
Sbjct: 365 HIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAV 424
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIKAV G +V K
Sbjct: 425 RDMRQTVAVGVIKAVEKVAPGAAKVTK 451
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +1
Query: 4 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTA 183
A +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTA
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTA 340
Query: 184 QVIVLNHPGQISNGYTPVLDCHTA 255
QVI+LNHPGQIS GY+PVLDCHTA
Sbjct: 341 QVIILNHPGQISAGYSPVLDCHTA 364
Score = 126 bits (304), Expect = 4e-30
Identities = 58/87 (66%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAV
Sbjct: 365 HIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAV 424
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIKAV G +V K
Sbjct: 425 RDMRQTVAVGVIKAVEKVAPGAAKVTK 451
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +1
Query: 4 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTA 183
A +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTA
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTA 340
Query: 184 QVIVLNHPGQISNGYTPVLDCHTA 255
QVI+LNHPGQIS GY+PVLDCHTA
Sbjct: 341 QVIILNHPGQISAGYSPVLDCHTA 364
Score = 126 bits (304), Expect = 4e-30
Identities = 58/87 (66%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAV
Sbjct: 365 HIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAV 424
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIKAV G +V K
Sbjct: 425 RDMRQTVAVGVIKAVEKVAPGAAKVTK 451
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 49.6 bits (113), Expect = 5e-07
Identities = 28/82 (34%), Positives = 46/82 (56%), Gaps = 4/82 (4%)
Frame = +3
Query: 249 HCHIACK---FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 419
H H A + FA++ K+D+ T + ++ P G I L P+C+E F+++ +
Sbjct: 582 HIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYM 640
Query: 420 GRFAVRDMRQTVAVG-VIKAVN 482
GRF +RD TVAVG V+K ++
Sbjct: 641 GRFTLRDQGTTVAVGKVVKILD 662
Score = 43.2 bits (97), Expect = 4e-05
Identities = 26/84 (30%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = +1
Query: 7 NITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTA 183
N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F A
Sbjct: 505 NQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIA 562
Query: 184 QVIVLNHPGQISNGYTPVLDCHTA 255
Q+ +L P ++ GY+ V+ HTA
Sbjct: 563 QIAILELPSILTTGYSCVMHIHTA 586
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +1
Query: 4 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 132
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.11
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +3
Query: 381 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 473
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 117 QFLDGHVLYVETYIVSRYSFLESFV 43
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 325
Score = 26.6 bits (56), Expect = 4.0
Identities = 20/51 (39%), Positives = 24/51 (47%)
Frame = -2
Query: 370 TKLTMAASPDLMDFGLTSVDLPVRRSTFSLISANLQAMWQCGNPILVCNRL 218
T L+ AA P L D TSV + + SL SA A PI+VC L
Sbjct: 256 TSLSEAALPPLTDVQRTSVLSLLTSTLHSLESARQIADSNTWKPIIVCLEL 306
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 26.2 bits (55), Expect = 5.3
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = -2
Query: 358 MAASPDLMDFGLTSVDLPVRRSTFSLISANLQAMWQCGNPILVCNRLRFDQDG 200
++ +PDL D L+SVD P++ +T S + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS----------ESGNQYLLCD---FNRDG 66
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 7.0
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -3
Query: 141 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 25
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,203,195
Number of Sequences: 5004
Number of extensions: 65539
Number of successful extensions: 181
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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