Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30950
(779 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 146 1e-35
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 146 1e-35
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 146 1e-35
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 146 1e-35
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 105 2e-23
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 57 1e-08
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 48 5e-06
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 35 0.070
At4g27900.2 68417.m04005 expressed protein 32 0.49
At4g27900.1 68417.m04004 expressed protein 32 0.49
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 32 0.49
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 30 1.5
At5g42860.1 68418.m05224 expressed protein 25 7.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 146 bits (355), Expect = 1e-35
Identities = 63/82 (76%), Positives = 77/82 (93%)
Frame = +1
Query: 10 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 189
+TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QV
Sbjct: 273 LTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQV 332
Query: 190 IVLNHPGQISNGYTPVLDCHTA 255
I++NHPGQI NGY PVLDCHT+
Sbjct: 333 IIMNHPGQIGNGYAPVLDCHTS 354
Score = 118 bits (285), Expect = 3e-27
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAV
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAV 414
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIK+V+ K+ G +V K
Sbjct: 415 RDMRQTVAVGVIKSVDKKDPTGAKVTK 441
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 146 bits (355), Expect = 1e-35
Identities = 63/82 (76%), Positives = 77/82 (93%)
Frame = +1
Query: 10 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 189
+TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QV
Sbjct: 273 LTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQV 332
Query: 190 IVLNHPGQISNGYTPVLDCHTA 255
I++NHPGQI NGY PVLDCHT+
Sbjct: 333 IIMNHPGQIGNGYAPVLDCHTS 354
Score = 118 bits (285), Expect = 3e-27
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAV
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAV 414
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIK+V+ K+ G +V K
Sbjct: 415 RDMRQTVAVGVIKSVDKKDPTGAKVTK 441
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 146 bits (355), Expect = 1e-35
Identities = 63/82 (76%), Positives = 77/82 (93%)
Frame = +1
Query: 10 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 189
+TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QV
Sbjct: 273 LTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQV 332
Query: 190 IVLNHPGQISNGYTPVLDCHTA 255
I++NHPGQI NGY PVLDCHT+
Sbjct: 333 IIMNHPGQIGNGYAPVLDCHTS 354
Score = 118 bits (285), Expect = 3e-27
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAV
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAV 414
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIK+V+ K+ G +V K
Sbjct: 415 RDMRQTVAVGVIKSVDKKDPTGAKVTK 441
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 146 bits (355), Expect = 1e-35
Identities = 63/82 (76%), Positives = 77/82 (93%)
Frame = +1
Query: 10 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 189
+TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QV
Sbjct: 273 LTTEVKSVEMHHESLLEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQV 332
Query: 190 IVLNHPGQISNGYTPVLDCHTA 255
I++NHPGQI NGY PVLDCHT+
Sbjct: 333 IIMNHPGQIGNGYAPVLDCHTS 354
Score = 118 bits (285), Expect = 3e-27
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIA KF+EI K+DRR+GK E PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAV
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAV 414
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGRVLK 515
RDMRQTVAVGVIK+V+ K+ G +V K
Sbjct: 415 RDMRQTVAVGVIKSVDKKDPTGAKVTK 441
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 105 bits (253), Expect = 2e-23
Identities = 47/84 (55%), Positives = 63/84 (75%)
Frame = +3
Query: 255 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 434
HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P+KP+ VE++ +PPLGRFA+
Sbjct: 21 HIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGRFAI 80
Query: 435 RDMRQTVAVGVIKAVNFKEAGGGR 506
RDMRQTV VGVIK+V K+ G +
Sbjct: 81 RDMRQTVGVGVIKSVVKKDPSGAK 104
Score = 42.7 bits (96), Expect = 3e-04
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1
Query: 196 LNHPGQISNGYTPVLDCHTA 255
+NH GQI NGYTPVLDCHT+
Sbjct: 1 MNHLGQIKNGYTPVLDCHTS 20
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 57.2 bits (132), Expect = 1e-08
Identities = 23/69 (33%), Positives = 41/69 (59%)
Frame = +3
Query: 264 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 443
C+ E+K ++D +T K + +K+G A + + + +C+E F +FP LGRF +R
Sbjct: 454 CEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTE 513
Query: 444 RQTVAVGVI 470
+T+AVG +
Sbjct: 514 GKTIAVGKV 522
Score = 30.3 bits (65), Expect = 1.5
Identities = 18/79 (22%), Positives = 37/79 (46%), Gaps = 2/79 (2%)
Frame = +1
Query: 19 EVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVL 198
+V ++ + ++ A PG+N+ + + +++ G+V S NP +F AQ+ +L
Sbjct: 371 KVVAIYCDEDKVKRAGPGENLRVRITGIEDEDILSGFVL-SSIVNPVPAVTEFVAQLQIL 429
Query: 199 N--HPGQISNGYTPVLDCH 249
+ GY +L H
Sbjct: 430 ELLDNAIFTAGYKAILHIH 448
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 48.4 bits (110), Expect = 5e-06
Identities = 22/77 (28%), Positives = 43/77 (55%)
Frame = +3
Query: 249 HCHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 428
H A ++ +D +TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 587 HAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRV 646
Query: 429 AVRDMRQTVAVGVIKAV 479
+R +TVA+G + +
Sbjct: 647 FLRSSGRTVAMGKVTRI 663
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 34.7 bits (76), Expect = 0.070
Identities = 15/37 (40%), Positives = 23/37 (62%)
Frame = +1
Query: 22 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 132
V VEM + L EA+ GDNVG ++ + +++RG V
Sbjct: 333 VTGVEMFQKILDEALAGDNVGLLLRGIQKADIQRGMV 369
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.49
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 327 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 419
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.49
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = +3
Query: 327 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 419
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 31.9 bits (69), Expect = 0.49
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +1
Query: 10 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQV 189
+ + V VEM + L GDNVG ++ + ++++RG V +K K F A++
Sbjct: 312 LKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVI--AKPGSCKTYKKFEAEI 369
Query: 190 IVL 198
VL
Sbjct: 370 YVL 372
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +3
Query: 243 LPHCHIAC-KFAEIKEKVDRRTGKSTEVNPKSI 338
+P+ H +F + KEK+DR GK E+N K +
Sbjct: 249 VPYAHYGLMRFPKPKEKIDREGGKPLEINVKKL 281
>At5g42860.1 68418.m05224 expressed protein
Length = 320
Score = 25.0 bits (52), Expect(2) = 7.1
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +1
Query: 520 RKGHQGQEVARAVNSTIFHTTAILHSPKGFSKEKRATNS 636
R H G++ A T FH+T +L SP G +++S
Sbjct: 33 RDSHDGEKTA-----TSFHSTPVLTSPMGSPPHSHSSSS 66
Score = 21.4 bits (43), Expect(2) = 7.1
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +1
Query: 376 PSLYV*SPSRNSH 414
P+ +V SPSR+SH
Sbjct: 24 PAYFVQSPSRDSH 36
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,920,852
Number of Sequences: 28952
Number of extensions: 350839
Number of successful extensions: 988
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 949
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 988
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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