Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30940
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g20290.1 68416.m02571 calcium-binding EF hand family protein ... 107 6e-24
At4g05520.2 68417.m00839 calcium-binding EF hand family protein ... 105 4e-23
At4g05520.1 68417.m00838 calcium-binding EF hand family protein ... 105 4e-23
At1g47350.1 68414.m05242 F-box family protein-related contains T... 30 1.8
At4g30790.1 68417.m04362 expressed protein 29 3.2
At3g03090.1 68416.m00305 sugar transporter family protein simila... 29 3.2
At2g28560.1 68415.m03470 expressed protein 29 3.2
At5g16720.1 68418.m01958 expressed protein contains Pfam profile... 29 4.2
At3g27260.1 68416.m03407 DNA-binding bromodomain-containing prot... 29 4.2
At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein... 27 9.6
At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate mu... 27 9.6
At2g05360.1 68415.m00564 hypothetical protein 27 9.6
At1g78930.1 68414.m09202 mitochondrial transcription termination... 27 9.6
>At3g20290.1 68416.m02571 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 107 bits (258), Expect = 6e-24
Identities = 51/79 (64%), Positives = 61/79 (77%), Gaps = 4/79 (5%)
Frame = +2
Query: 2 DKIRIVLNKADMIDHQQLMRVYGALMWSLGKVLQTPEVARVYIGSFWDEPLRY----DVN 169
DKIR+VLNKAD +D QQLMRVYGALMWSLGKVL TPEV+RVYIGSF D+P+ +
Sbjct: 351 DKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVSRVYIGSFSDKPINEAATGPIG 410
Query: 170 RRLFEDEEQDLFKDMQSLP 226
R LFE E+ DL D++ +P
Sbjct: 411 RELFEKEQDDLLADLKDIP 429
Score = 88.6 bits (210), Expect = 4e-18
Identities = 41/108 (37%), Positives = 64/108 (59%)
Frame = +1
Query: 226 KECCPA*TKRLIKRARLAKVHAYIVNELRKEMPSMFGKDSKKKDLIKNLGQVYDKIQREQ 405
K+ C +KRAR AK+HAYI++ L+KEMP++ GK ++ LI NL + K+QRE
Sbjct: 430 KKACDRRINEFVKRARAAKIHAYIISHLKKEMPAIMGKAKAQQKLIDNLEDEFGKVQREH 489
Query: 406 QISPGDFPDIKKMQETLGQS*LHQVSPLETQVARIVDHMLATDIARLM 549
+ GDFP++ +E L + + L+ ++ + VD ML DI L+
Sbjct: 490 HLPKGDFPNVDHFREVLSGYNIDKFEKLKPKMLQTVDDMLGYDIPELL 537
>At4g05520.2 68417.m00839 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4, {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 105 bits (251), Expect = 4e-23
Identities = 50/79 (63%), Positives = 59/79 (74%), Gaps = 4/79 (5%)
Frame = +2
Query: 2 DKIRIVLNKADMIDHQQLMRVYGALMWSLGKVLQTPEVARVYIGSFWDEPLRY----DVN 169
DKIR+VLNKAD +D QQLMRVYGALMWSLGKVL TPEV RVYIGSF D+P+ +
Sbjct: 350 DKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPINEVAVGPIG 409
Query: 170 RRLFEDEEQDLFKDMQSLP 226
+ LFE E+ DL D+ +P
Sbjct: 410 KELFEKEQNDLLADLMDVP 428
Score = 87.0 bits (206), Expect = 1e-17
Identities = 41/108 (37%), Positives = 66/108 (61%)
Frame = +1
Query: 226 KECCPA*TKRLIKRARLAKVHAYIVNELRKEMPSMFGKDSKKKDLIKNLGQVYDKIQREQ 405
K+ C +KRAR AK++AYI++ L+KEMP+M GK ++ L+ NL + + K+QRE
Sbjct: 429 KKACDRKINEFVKRARSAKINAYIMSHLKKEMPAMMGKSKAQQRLMDNLEEEFGKVQREF 488
Query: 406 QISPGDFPDIKKMQETLGQS*LHQVSPLETQVARIVDHMLATDIARLM 549
+ GDFP ++ +E LG + + L+ ++ + VD ML DI L+
Sbjct: 489 HLPAGDFPSVEHFREVLGGYNIDKFEKLKPKMIQAVDDMLGYDIPDLL 536
>At4g05520.1 68417.m00838 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4, {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 546
Score = 105 bits (251), Expect = 4e-23
Identities = 50/79 (63%), Positives = 59/79 (74%), Gaps = 4/79 (5%)
Frame = +2
Query: 2 DKIRIVLNKADMIDHQQLMRVYGALMWSLGKVLQTPEVARVYIGSFWDEPLRY----DVN 169
DKIR+VLNKAD +D QQLMRVYGALMWSLGKVL TPEV RVYIGSF D+P+ +
Sbjct: 351 DKIRVVLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPINEVAVGPIG 410
Query: 170 RRLFEDEEQDLFKDMQSLP 226
+ LFE E+ DL D+ +P
Sbjct: 411 KELFEKEQNDLLADLMDVP 429
Score = 87.0 bits (206), Expect = 1e-17
Identities = 41/108 (37%), Positives = 66/108 (61%)
Frame = +1
Query: 226 KECCPA*TKRLIKRARLAKVHAYIVNELRKEMPSMFGKDSKKKDLIKNLGQVYDKIQREQ 405
K+ C +KRAR AK++AYI++ L+KEMP+M GK ++ L+ NL + + K+QRE
Sbjct: 430 KKACDRKINEFVKRARSAKINAYIMSHLKKEMPAMMGKSKAQQRLMDNLEEEFGKVQREF 489
Query: 406 QISPGDFPDIKKMQETLGQS*LHQVSPLETQVARIVDHMLATDIARLM 549
+ GDFP ++ +E LG + + L+ ++ + VD ML DI L+
Sbjct: 490 HLPAGDFPSVEHFREVLGGYNIDKFEKLKPKMIQAVDDMLGYDIPDLL 537
>At1g47350.1 68414.m05242 F-box family protein-related contains
TIGR01640: F-box protein interaction domain
Length = 528
Score = 29.9 bits (64), Expect = 1.8
Identities = 18/66 (27%), Positives = 32/66 (48%), Gaps = 3/66 (4%)
Frame = +1
Query: 427 PDIKKMQ--ETLGQS*LHQVSPLETQVARIVDHMLATDIARLMDMIPQ-EDINIVSEPLI 597
P++ +Q E G H+ S ++V VD++ D +R + EDIN++ L+
Sbjct: 406 PEMNTLQCVEIRGFGEYHEASGNRSRVCVFVDNVFEKDCSRFYALADHVEDINVIDSKLL 465
Query: 598 KGGAFE 615
K +E
Sbjct: 466 KSKIYE 471
>At4g30790.1 68417.m04362 expressed protein
Length = 1148
Score = 29.1 bits (62), Expect = 3.2
Identities = 18/68 (26%), Positives = 34/68 (50%)
Frame = +1
Query: 253 RLIKRARLAKVHAYIVNELRKEMPSMFGKDSKKKDLIKNLGQVYDKIQREQQISPGDFPD 432
R I++ R AK+H Y+ E RK + +D+ KK ++N + + + + F +
Sbjct: 215 RDIEKLRSAKIHPYLQTESRKCLLDFVKEDNLKK-AVENCASSHRQFENKIAQFQQMFVE 273
Query: 433 IKKMQETL 456
+K+ E L
Sbjct: 274 VKRKVEEL 281
>At3g03090.1 68416.m00305 sugar transporter family protein similar
to xylose permease [Bacillus megaterium] GI:1924928;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 503
Score = 29.1 bits (62), Expect = 3.2
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = -3
Query: 608 APPFIRGSLTMLMSSCGIISMSRAMSVASMWSTILATW 495
AP IRG + L ++ M + S+W T+++ W
Sbjct: 173 APSQIRGRMISLKEFSTVLGMVGGYGIGSLWITVISGW 210
>At2g28560.1 68415.m03470 expressed protein
Length = 320
Score = 29.1 bits (62), Expect = 3.2
Identities = 14/44 (31%), Positives = 21/44 (47%), Gaps = 2/44 (4%)
Frame = -1
Query: 700 ADPFRVTVAGVHALRPDRNRMVRPGL--LHLRKHHPLSEVH*QC 575
A PF +T AG+ L + + PG+ +H R H + H C
Sbjct: 276 AFPFHITSAGISLLSDNGTELKGPGINTIHARGHSDMINFHGDC 319
>At5g16720.1 68418.m01958 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 675
Score = 28.7 bits (61), Expect = 4.2
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 331 FGKDSKKKDLIKNLGQVYDKIQ 396
FG DS+K ++IK + VY+++Q
Sbjct: 601 FGSDSEKLEIIKQVDSVYERLQ 622
>At3g27260.1 68416.m03407 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 813
Score = 28.7 bits (61), Expect = 4.2
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +1
Query: 436 KKMQETLGQS*LHQVSPLETQVARIVDHMLATDIARLMDMIPQEDINIVSEPLIKGGAFE 615
+KM + +S V PL T+V R H L + L+D +P I+ + + GG E
Sbjct: 312 RKMASPVRESVPEPVKPLMTEVER---HRLGRQLESLLDELPAHIIDFLKKHNSNGG--E 366
Query: 616 GVEDQV 633
ED++
Sbjct: 367 IAEDEI 372
>At3g48890.1 68416.m05341 cytochrome b5 domain-containing protein
similar to SP|O00264 Membrane associated progesterone
receptor component (mPR) {Homo sapiens}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 233
Score = 27.5 bits (58), Expect = 9.6
Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = +1
Query: 298 VNELRKEMPSMF-GKDSKKKDLIKNLGQVYDKIQREQQISPG 420
+ E+ +E ++ G DSKK L+ GQ+YD Q PG
Sbjct: 68 LGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPG 109
>At3g26780.1 68416.m03350 phosphoglycerate/bisphosphoglycerate
mutase family protein similar to X4 protein GI:21386798,
Y4 protein GI:21386800 from [Silene dioica]; contains
Pfam profiles PF00300: phosphoglycerate mutase family,
PF01535: PPR repeat
Length = 1053
Score = 27.5 bits (58), Expect = 9.6
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2
Query: 606 TTLYQRFTDNVDVLLWNHI 550
TTL+ R+ D DV WN +
Sbjct: 423 TTLFNRYVDKTDVFSWNSV 441
>At2g05360.1 68415.m00564 hypothetical protein
Length = 358
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = +3
Query: 81 GHWEKFYKLLRLLECTLAHFGMSLSGTMLIVDFLKMKNKIYLKTCSLCQGMLPCVN*T 254
G++ + YK ++ + H L + +DFL + +K C G+L CVN T
Sbjct: 55 GYFLESYKGGYTIDTSFVHERRDLENKGVSIDFLPQGER-KIKACDASHGILLCVNDT 111
>At1g78930.1 68414.m09202 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 591
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -3
Query: 161 RTGEAHPKMSQCTL*QPQEFVKL 93
R G+ PKM Q L +PQEF+K+
Sbjct: 387 RMGKVIPKMPQLLLCKPQEFLKV 409
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,199,919
Number of Sequences: 28952
Number of extensions: 348904
Number of successful extensions: 967
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 937
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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