Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30933
(739 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 156 4e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 155 8e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 76 5e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 6e-08
SPBC725.03 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.52
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 28 1.6
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 8.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 156 bits (378), Expect = 4e-39
Identities = 81/137 (59%), Positives = 99/137 (72%)
Frame = +2
Query: 254 LERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISA 433
+ERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA
Sbjct: 219 IERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSA 278
Query: 434 EKAYHEQLSVAEITNACFRAPAKPDVKCDPRSMGKYMGLPVCCNRGDVVTPRM*KAGNRL 613
KA+HE SV EITN CF P VKCDPR+ G+YM RGDV+ PR +A
Sbjct: 279 AKAFHESNSVQEITNQCFE-PYNQMVKCDPRA-GRYMA-TCLLYRGDVI-PRDVQAAVTT 334
Query: 614 TSKNQSGNYSNFVGTGW 664
++ + ++ TG+
Sbjct: 335 IKAKRTIQFVDWCPTGF 351
Score = 124 bits (299), Expect = 1e-29
Identities = 54/85 (63%), Positives = 68/85 (80%)
Frame = +3
Query: 3 FLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTT 182
FL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH T
Sbjct: 135 FLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHAT 194
Query: 183 LEHSDCAFMVDNEAIYDICRRNLDL 257
L+ +DC FMVDNE+ YDICRRNLD+
Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDI 219
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 155 bits (375), Expect = 8e-39
Identities = 81/137 (59%), Positives = 98/137 (71%)
Frame = +2
Query: 254 LERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISA 433
+ERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA
Sbjct: 223 IERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSA 282
Query: 434 EKAYHEQLSVAEITNACFRAPAKPDVKCDPRSMGKYMGLPVCCNRGDVVTPRM*KAGNRL 613
KA+HE SV EITN CF P VKCDPR+ G+YM RGDV+ PR +A
Sbjct: 283 AKAFHESNSVQEITNQCFE-PYNQMVKCDPRT-GRYMA-TCLLYRGDVI-PRDVQAAVTS 338
Query: 614 TSKNQSGNYSNFVGTGW 664
++ + ++ TG+
Sbjct: 339 IKSRRTIQFVDWCPTGF 355
Score = 130 bits (314), Expect = 2e-31
Identities = 58/90 (64%), Positives = 72/90 (80%)
Frame = +3
Query: 3 FLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTT 182
FL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH T
Sbjct: 139 FLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHAT 198
Query: 183 LEHSDCAFMVDNEAIYDICRRNLDLSAQPT 272
L++SDC FMVDNEA YDICRRNLD+ +PT
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIE-RPT 227
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 76.2 bits (179), Expect = 5e-15
Identities = 36/98 (36%), Positives = 60/98 (61%)
Frame = +2
Query: 251 RLERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS 430
+++ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ +
Sbjct: 216 KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAA 275
Query: 431 AEKAYHEQLSVAEITNACFRAPAKPDVKCDPRSMGKYM 544
+ + +SV E+T F A V DPR G+Y+
Sbjct: 276 IGSSSFQAVSVPELTQQMFDA-NNMMVAADPRH-GRYL 311
Score = 58.4 bits (135), Expect = 1e-09
Identities = 26/70 (37%), Positives = 42/70 (60%)
Frame = +3
Query: 54 LLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYD 233
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+
Sbjct: 150 LLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSS 209
Query: 234 ICRRNLDLSA 263
I L + +
Sbjct: 210 IFANTLKIKS 219
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.4 bits (120), Expect = 6e-08
Identities = 21/55 (38%), Positives = 34/55 (61%)
Frame = +2
Query: 257 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 421
+ PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P
Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275
Score = 44.0 bits (99), Expect = 2e-05
Identities = 26/84 (30%), Positives = 40/84 (47%), Gaps = 1/84 (1%)
Frame = +3
Query: 3 FLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHT 179
F + H L+ERL+ Y KK ++++P Q VS VV+PYNS+L
Sbjct: 135 FSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKR 194
Query: 180 TLEHSDCAFMVDNEAIYDICRRNL 251
++D ++DN A+ I L
Sbjct: 195 LTLNADSVVVLDNAALAHIAADRL 218
>SPBC725.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 257
Score = 29.5 bits (63), Expect = 0.52
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 602 GNRLTSKNQSGNYSNFVGTGWSKP 673
GN + +N++ FVGTGW KP
Sbjct: 36 GNEIYPRNRTVAIRGFVGTGWHKP 59
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 27.9 bits (59), Expect = 1.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +3
Query: 522 PGQWASTWACLYAVTVVTSLPQGCERRAI 608
PGQ + TW+ + ++ + SLP +R I
Sbjct: 436 PGQSSDTWSIFWNISSLNSLPSSLSKREI 464
>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 530
Score = 25.4 bits (53), Expect = 8.5
Identities = 8/23 (34%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -3
Query: 704 WVS*IPNFGNPVWTTQFR-RNWN 639
+++ + N+G P+W T+F NW+
Sbjct: 443 YINSLANYGLPIWLTEFACTNWD 465
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,301,989
Number of Sequences: 5004
Number of extensions: 71890
Number of successful extensions: 201
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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