Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30918
(710 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g34250.1 68415.m04190 protein transport protein sec61, putati... 142 2e-34
At1g29310.1 68414.m03583 protein transport protein sec61, putati... 142 2e-34
At1g78720.1 68414.m09175 protein transport protein sec61, putati... 142 3e-34
At3g50180.1 68416.m05486 hypothetical protein 29 2.3
At5g10030.1 68418.m01162 bZIP family transcription factor (OBF4... 29 3.0
At4g27520.1 68417.m03952 plastocyanin-like domain-containing pro... 29 3.0
At5g28910.1 68418.m03564 expressed protein 28 5.3
At3g32400.1 68416.m04142 formin homology 2 domain-containing pro... 28 5.3
At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase... 28 7.0
At3g45090.1 68416.m04863 2-phosphoglycerate kinase-related conta... 28 7.0
At2g25050.1 68415.m02996 formin homology 2 domain-containing pro... 28 7.0
At2g42160.1 68415.m05218 zinc finger (ubiquitin-hydrolase) domai... 27 9.3
At2g20990.1 68415.m02485 C2 domain-containing protein (sytA) sim... 27 9.3
>At2g34250.1 68415.m04190 protein transport protein sec61, putative
similar to PfSec61 [Plasmodium falciparum] GI:3057044;
contains Pfam profile PF00344: eubacterial secY protein
Length = 475
Score = 142 bits (345), Expect = 2e-34
Identities = 68/82 (82%), Positives = 77/82 (93%)
Frame = +2
Query: 257 SSAKDVAKQLKEQQMVMRGHRDNSMIHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSG 436
SSA+DVAKQLKEQQMVM GHR++++ ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSG
Sbjct: 386 SSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSG 445
Query: 437 TGILLAVTIIYQYFEIFVKEQA 502
TGILLAVTIIYQYFE F KE+A
Sbjct: 446 TGILLAVTIIYQYFETFEKEKA 467
Score = 83.8 bits (198), Expect = 1e-16
Identities = 41/84 (48%), Positives = 52/84 (61%)
Frame = +3
Query: 3 NLYVISQMLAVKFSGNFLVNVLGVWADXXXXXXXXXXXXXXLCYYLSPPESLAHIAHDPV 182
NLY ISQ+L KFSGNF VN+LG W + L Y ++ P S + +A P
Sbjct: 303 NLYFISQLLYRKFSGNFFVNLLGQWKESEYSGQSIPVSG--LAYLITAPASFSDMAAHPF 360
Query: 183 HALLYVLFMLGSCAFFSKTWIDVS 254
HAL Y++FML +CA FSKTWI+VS
Sbjct: 361 HALFYIVFMLTACALFSKTWIEVS 384
>At1g29310.1 68414.m03583 protein transport protein sec61, putative
similar to PfSec61 [Plasmodium falciparum] GI:3057044;
contains Pfam profile PF00344: eubacterial secY protein
Length = 475
Score = 142 bits (345), Expect = 2e-34
Identities = 68/82 (82%), Positives = 77/82 (93%)
Frame = +2
Query: 257 SSAKDVAKQLKEQQMVMRGHRDNSMIHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSG 436
SSA+DVAKQLKEQQMVM GHR++++ ELNRYIPTAAAFGG+CIGAL+VLADF+GAIGSG
Sbjct: 386 SSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGVCIGALTVLADFMGAIGSG 445
Query: 437 TGILLAVTIIYQYFEIFVKEQA 502
TGILLAVTIIYQYFE F KE+A
Sbjct: 446 TGILLAVTIIYQYFETFEKEKA 467
Score = 85.0 bits (201), Expect = 4e-17
Identities = 42/84 (50%), Positives = 52/84 (61%)
Frame = +3
Query: 3 NLYVISQMLAVKFSGNFLVNVLGVWADXXXXXXXXXXXXXXLCYYLSPPESLAHIAHDPV 182
NLY ISQ+L KFSGNF VN+LG W + L Y ++ P S A +A P
Sbjct: 303 NLYFISQLLYRKFSGNFFVNLLGQWKESEYSGQSIPVSG--LAYLITAPASFADMAAHPF 360
Query: 183 HALLYVLFMLGSCAFFSKTWIDVS 254
HAL Y++FML +CA FSKTWI+VS
Sbjct: 361 HALFYIVFMLTACALFSKTWIEVS 384
>At1g78720.1 68414.m09175 protein transport protein sec61, putative
similar to SP|P38377 Protein transport protein Sec61
alpha subunit isoform 1 (Sec61 alpha- 1) {Canis
familiaris}; contains Pfam profile PF00344: eubacterial
secY protein
Length = 475
Score = 142 bits (343), Expect = 3e-34
Identities = 68/82 (82%), Positives = 76/82 (92%)
Frame = +2
Query: 257 SSAKDVAKQLKEQQMVMRGHRDNSMIHELNRYIPTAAAFGGLCIGALSVLADFLGAIGSG 436
SSAKDVA+QL+EQQMVM GHRD+++ ELNRYIPTAAAFGGLCIGAL+VLAD +GAIGSG
Sbjct: 386 SSAKDVARQLREQQMVMPGHRDSNLQKELNRYIPTAAAFGGLCIGALTVLADLMGAIGSG 445
Query: 437 TGILLAVTIIYQYFEIFVKEQA 502
TGILLAVTIIYQYFE F KE+A
Sbjct: 446 TGILLAVTIIYQYFETFEKEKA 467
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/89 (47%), Positives = 55/89 (61%)
Frame = +3
Query: 3 NLYVISQMLAVKFSGNFLVNVLGVWADXXXXXXXXXXXXXXLCYYLSPPESLAHIAHDPV 182
N+Y ISQ+L KF GNFLVN++G W + + YY++ P SLA +A P
Sbjct: 303 NIYFISQILYRKFGGNFLVNLIGTWKESEYSGQSIPVGG--IAYYITAPSSLAEMATHPF 360
Query: 183 HALLYVLFMLGSCAFFSKTWIDVSAPRPK 269
HAL Y++FML +CA FSKTWI+VS K
Sbjct: 361 HALFYLVFMLAACALFSKTWIEVSGSSAK 389
>At3g50180.1 68416.m05486 hypothetical protein
Length = 588
Score = 29.5 bits (63), Expect = 2.3
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -3
Query: 462 MVTASSIPVPEPMAPRKSASTDRAPMHSPP 373
M T +IP P P+ PR RAP PP
Sbjct: 1 METFGTIPPPPPLPPRLELRRQRAPPPQPP 30
>At5g10030.1 68418.m01162 bZIP family transcription factor (OBF4)
identical to ocs-element binding factor 4 GI:414613 from
[Arabidopsis thaliana]
Length = 364
Score = 29.1 bits (62), Expect = 3.0
Identities = 16/72 (22%), Positives = 33/72 (45%)
Frame = +1
Query: 238 PGSMCLLLGQRCGETTEGTADGDARSPRQLHDPRTEPLHPDGGRVRRTVHRGSVSAGRLP 417
PGS+ + ++ +E T+ G +P + + HPD + R +R + RL
Sbjct: 39 PGSIIIPTNEKPDSLSEDTSHGTEGTPHKFDQEASTSRHPDKIQRRLAQNREAARKSRLR 98
Query: 418 RGHRLRHWDTAR 453
+ ++ +T+R
Sbjct: 99 KKAYVQQLETSR 110
>At4g27520.1 68417.m03952 plastocyanin-like domain-containing
protein similar to PIR|JC7196 phytocyanin-related
protein Pn14 {Ipomoea nil}; contains Pfam profile
PF02298: Plastocyanin-like domain
Length = 349
Score = 29.1 bits (62), Expect = 3.0
Identities = 17/38 (44%), Positives = 23/38 (60%), Gaps = 5/38 (13%)
Frame = -3
Query: 459 VTASSIPV---PEPMAPRKSAST--DRAPMHSPPNAAA 361
V+ SS P+ P PMAP+ S++ APM SPP + A
Sbjct: 227 VSPSSAPMTSPPAPMAPKSSSTIPPSSAPMTSPPGSMA 264
>At5g28910.1 68418.m03564 expressed protein
Length = 408
Score = 28.3 bits (60), Expect = 5.3
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1
Query: 356 GCSGSVRGSWSC 321
GC GS RG+WSC
Sbjct: 112 GCKGSFRGNWSC 123
>At3g32400.1 68416.m04142 formin homology 2 domain-containing
protein / FH2 domain-containing protein common family
members: At2g43800, At3g25500, At5g48360, At4g15200,
At3g05470, At3g07540, At5g07780, At5g07650 [Arabidopsis
thaliana];
Length = 488
Score = 28.3 bits (60), Expect = 5.3
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -3
Query: 450 SSIPVPEPMAPRKSASTDRAPMHSPP 373
SS P+P P+ P+K +T P PP
Sbjct: 29 SSSPLPPPLPPKKLLATTNTPPPPPP 54
>At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase,
putative / 1-deoxyxylulose-5-phosphate synthase,
putative / DXP-synthase, putative (DEF) (CLA1) identical
to SP|Q38854 Probable 1-deoxy-D-xylulose 5-phosphate
synthase, chloroplast precursor (EC 4.1.3.37)
(1-deoxyxylulose-5-phosphate synthase) (DXP synthase)
(DXPS). [Mouse-ear cress] {Arabidopsis thaliana}, DEF
(deficient in photosynthesis) protein [Arabidopsis
thaliana] GI:1399261
Length = 717
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2
Query: 496 FLDKDLEVLVNDGDGEQYPSAGADGP 419
+LD D+ V++ND P+A DGP
Sbjct: 235 YLDSDMIVILNDNKQVSLPTATLDGP 260
>At3g45090.1 68416.m04863 2-phosphoglycerate kinase-related contains
weak similarity to 2-phosphoglycerate kinase (GI:467751)
[Methanothermus fervidus]
Length = 717
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/72 (22%), Positives = 33/72 (45%)
Frame = -2
Query: 631 KTLHLMMSIQSRNDNKNGAERSGPTADIITKT*KXQ*CSCRHLRLFLDKDLEVLVNDGDG 452
K L++++ + +D+ NG + T ++ T + C RH + E++ ++G
Sbjct: 7 KVLYIVVREEGDDDDNNGDDSFRYTRPVLQSTLQLMGCKARHAFKISRRVFELIRSEGSC 66
Query: 451 EQYPSAGADGPE 416
P G + PE
Sbjct: 67 NTSPENGKE-PE 77
>At2g25050.1 68415.m02996 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02128
Length = 1111
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -3
Query: 450 SSIPVPEPMAPRKSASTDRAPMHSPP 373
SS P+P P+ P+K +T P PP
Sbjct: 618 SSSPLPPPLPPKKLLATTNPPPPPPP 643
>At2g42160.1 68415.m05218 zinc finger (ubiquitin-hydrolase)
domain-containing protein similar to BRCA1-associated
protein 2 [Homo sapiens] GI:3252872; contains Pfam
profile PF02148: Zn-finger in ubiquitin-hydrolases and
other protein
Length = 488
Score = 27.5 bits (58), Expect = 9.3
Identities = 18/80 (22%), Positives = 26/80 (32%), Gaps = 1/80 (1%)
Frame = +1
Query: 391 GSVSAGRLPRGHRLRHWDTARRHHHLPVLRDLCQGTSGDGGM-XXXXXXXXXXXYRQSDR 567
G V GR GH +RHW + L + GD + S
Sbjct: 241 GFVGCGRYKEGHSIRHWKETHHCYSLDLRTQQIWDYVGDSYVHRLNHSKIDGKSVEMSTS 300
Query: 568 CVQLRFYCRFCSESTSLGVT 627
C+ + C C S G++
Sbjct: 301 CLSHQGDCGLCECSEDTGIS 320
>At2g20990.1 68415.m02485 C2 domain-containing protein (sytA)
similar to Ca2+-dependent lipid-binding protein (CLB1)
GI:2789434 from [Lycopersicon esculentum]
Length = 541
Score = 27.5 bits (58), Expect = 9.3
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +2
Query: 239 LDRCVCSSAKDVAKQLKEQQM 301
LD+ +C +AK++AK + E+Q+
Sbjct: 85 LDKAICKTAKNIAKPIIEEQI 105
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,447,044
Number of Sequences: 28952
Number of extensions: 248590
Number of successful extensions: 818
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 814
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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