BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30905
(658 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr ... 37 0.002
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 30 0.26
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 29 0.78
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 28 1.4
SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.2
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 26 5.5
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 25 7.3
>SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 37.1 bits (82), Expect = 0.002
Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 2/56 (3%)
Frame = +1
Query: 94 NDHVLRLAYSSAIMRFINHMLDVSITKENTLSKA--AKNVGIPDWIVELRHDTAHK 255
ND +LA + +I RF++ +LD + + A AK++ +P + VELRH H+
Sbjct: 68 NDRSQKLALAVSISRFVSGLLDPIQQSQYAIPMAVLAKSIDLPTYFVELRHAITHE 123
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 30.3 bits (65), Expect = 0.26
Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = -1
Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPLSN 218
PNC AS ++++ RL+ G+CH+++ L N
Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKLEN 150
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 28.7 bits (61), Expect = 0.78
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 3/54 (5%)
Frame = +1
Query: 121 SSAIMRFINHMLD---VSITKENTLSKAAKNVGIPDWIVELRHDTAHKIICLHC 273
SS +M ML VS +E L+K KN + DW++ + I +HC
Sbjct: 394 SSNLMDIKQKMLQQKIVSYLEEKKLAKRVKNYRLKDWLISRQRFWGTPIPMVHC 447
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/27 (40%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = -1
Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPL 224
PNC AS ++++ RL+ G+CH+++ L
Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKL 148
>SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 217
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = -3
Query: 539 YHQLNPLHHLYRISH-INIHYVSQSF 465
YHQ+ H L+ ISH I+ H++ SF
Sbjct: 161 YHQIILSHSLFHISHLISFHFLFFSF 186
>SPAC6F6.12 |||autophagy associated protein
Atg24|Schizosaccharomyces pombe|chr 1|||Manual
Length = 401
Score = 25.8 bits (54), Expect = 5.5
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +3
Query: 369 RQETDSQEETRVMILVNFCISLSICSNSECKIK 467
R+E +T V L+ +LSI + +ECK++
Sbjct: 26 RKELQGSRDTHVSYLIITKTNLSIFTRAECKVR 58
>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 504
Score = 25.4 bits (53), Expect = 7.3
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = -3
Query: 266 RQIILWAVSCLNSTIQSGIPTFF 198
+ + +WA+ C+ T+ G P FF
Sbjct: 272 KNVDMWAMGCVLHTMLCGFPPFF 294
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,597,169
Number of Sequences: 5004
Number of extensions: 50056
Number of successful extensions: 157
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -