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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30905
         (658 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr ...    37   0.002
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...    30   0.26 
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy...    29   0.78 
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...    28   1.4  
SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   4.2  
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom...    26   5.5  
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz...    25   7.3  

>SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 470

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 2/56 (3%)
 Frame = +1

Query: 94  NDHVLRLAYSSAIMRFINHMLDVSITKENTLSKA--AKNVGIPDWIVELRHDTAHK 255
           ND   +LA + +I RF++ +LD     +  +  A  AK++ +P + VELRH   H+
Sbjct: 68  NDRSQKLALAVSISRFVSGLLDPIQQSQYAIPMAVLAKSIDLPTYFVELRHAITHE 123


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 30.3 bits (65), Expect = 0.26
 Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
 Frame = -1

Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPLSN 218
           PNC AS   ++++ RL+ G+CH+++ L N
Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKLEN 150


>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 874

 Score = 28.7 bits (61), Expect = 0.78
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 3/54 (5%)
 Frame = +1

Query: 121 SSAIMRFINHMLD---VSITKENTLSKAAKNVGIPDWIVELRHDTAHKIICLHC 273
           SS +M     ML    VS  +E  L+K  KN  + DW++  +      I  +HC
Sbjct: 394 SSNLMDIKQKMLQQKIVSYLEEKKLAKRVKNYRLKDWLISRQRFWGTPIPMVHC 447


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 11/27 (40%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
 Frame = -1

Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPL 224
           PNC AS   ++++ RL+ G+CH+++ L
Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKL 148


>SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 217

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
 Frame = -3

Query: 539 YHQLNPLHHLYRISH-INIHYVSQSF 465
           YHQ+   H L+ ISH I+ H++  SF
Sbjct: 161 YHQIILSHSLFHISHLISFHFLFFSF 186


>SPAC6F6.12 |||autophagy associated protein
           Atg24|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 401

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = +3

Query: 369 RQETDSQEETRVMILVNFCISLSICSNSECKIK 467
           R+E     +T V  L+    +LSI + +ECK++
Sbjct: 26  RKELQGSRDTHVSYLIITKTNLSIFTRAECKVR 58


>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
           Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 504

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 8/23 (34%), Positives = 14/23 (60%)
 Frame = -3

Query: 266 RQIILWAVSCLNSTIQSGIPTFF 198
           + + +WA+ C+  T+  G P FF
Sbjct: 272 KNVDMWAMGCVLHTMLCGFPPFF 294


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,597,169
Number of Sequences: 5004
Number of extensions: 50056
Number of successful extensions: 157
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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