BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30905 (658 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr ... 37 0.002 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 30 0.26 SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 29 0.78 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 28 1.4 SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.2 SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 26 5.5 SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz... 25 7.3 >SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 37.1 bits (82), Expect = 0.002 Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 2/56 (3%) Frame = +1 Query: 94 NDHVLRLAYSSAIMRFINHMLDVSITKENTLSKA--AKNVGIPDWIVELRHDTAHK 255 ND +LA + +I RF++ +LD + + A AK++ +P + VELRH H+ Sbjct: 68 NDRSQKLALAVSISRFVSGLLDPIQQSQYAIPMAVLAKSIDLPTYFVELRHAITHE 123 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 30.3 bits (65), Expect = 0.26 Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 1/29 (3%) Frame = -1 Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPLSN 218 PNC AS ++++ RL+ G+CH+++ L N Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKLEN 150 >SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 874 Score = 28.7 bits (61), Expect = 0.78 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 3/54 (5%) Frame = +1 Query: 121 SSAIMRFINHMLD---VSITKENTLSKAAKNVGIPDWIVELRHDTAHKIICLHC 273 SS +M ML VS +E L+K KN + DW++ + I +HC Sbjct: 394 SSNLMDIKQKMLQQKIVSYLEEKKLAKRVKNYRLKDWLISRQRFWGTPIPMVHC 447 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 27.9 bits (59), Expect = 1.4 Identities = 11/27 (40%), Positives = 20/27 (74%), Gaps = 1/27 (3%) Frame = -1 Query: 301 PNCVAS-LSSDNEGRLFYGQCHVSIPL 224 PNC AS ++++ RL+ G+CH+++ L Sbjct: 122 PNCGASTFMANHKDRLYCGRCHLTLKL 148 >SPAC17A2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 217 Score = 26.2 bits (55), Expect = 4.2 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = -3 Query: 539 YHQLNPLHHLYRISH-INIHYVSQSF 465 YHQ+ H L+ ISH I+ H++ SF Sbjct: 161 YHQIILSHSLFHISHLISFHFLFFSF 186 >SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = +3 Query: 369 RQETDSQEETRVMILVNFCISLSICSNSECKIK 467 R+E +T V L+ +LSI + +ECK++ Sbjct: 26 RKELQGSRDTHVSYLIITKTNLSIFTRAECKVR 58 >SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 25.4 bits (53), Expect = 7.3 Identities = 8/23 (34%), Positives = 14/23 (60%) Frame = -3 Query: 266 RQIILWAVSCLNSTIQSGIPTFF 198 + + +WA+ C+ T+ G P FF Sbjct: 272 KNVDMWAMGCVLHTMLCGFPPFF 294 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,597,169 Number of Sequences: 5004 Number of extensions: 50056 Number of successful extensions: 157 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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