Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30880
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 71 1e-13
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 5e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 1e-04
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 37 0.004
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.005
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 33 0.036
SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25 |Sch... 30 0.44
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 29 0.58
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 28 1.3
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 28 1.8
SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 27 2.3
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 4.1
SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subu... 25 9.5
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 25 9.5
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 25 9.5
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 71.3 bits (167), Expect = 1e-13
Identities = 29/63 (46%), Positives = 46/63 (73%)
Frame = +1
Query: 283 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 462
++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 463 KEL 471
K +
Sbjct: 132 KPI 134
Score = 48.4 bits (110), Expect = 1e-06
Identities = 21/51 (41%), Positives = 29/51 (56%)
Frame = +2
Query: 104 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAA 256
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAA 65
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/43 (46%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +2
Query: 119 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 244
++ED++ L +F V+ + LV FYAPWCGHCK L P Y
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTY 395
Score = 32.7 bits (71), Expect = 0.062
Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Frame = +1
Query: 268 TDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM- 438
+DD V +AK+D TE S S+SG+PT+ F+ + + Y G R + ++
Sbjct: 403 SDDSNVVVAKIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFID 458
Query: 439 -RAQVGPSSKE 468
A P KE
Sbjct: 459 KHASFEPIKKE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 49.6 bits (113), Expect = 5e-07
Identities = 23/73 (31%), Positives = 38/73 (52%)
Frame = +2
Query: 41 KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 220
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 221 CKRLKPEYAVAAA 259
CK+L P Y A+
Sbjct: 63 CKKLVPTYQKLAS 75
Score = 39.5 bits (88), Expect = 5e-04
Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
Frame = +1
Query: 298 VDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE--LSSEYNGPRESNGIVKYMRAQVGPS 459
VDC + ++ C Q+ V G+PT+K+ KG S++YNG R + K++ + PS
Sbjct: 86 VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PS 144
Query: 460 SKELLT 477
++LT
Sbjct: 145 KVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 41.5 bits (93), Expect = 1e-04
Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +2
Query: 128 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEY 244
+V++L +F V+ LV FYA WCG+CKRL P Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 39.1 bits (87), Expect = 7e-04
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +2
Query: 185 ALVMFYAPWCGHCKRLKPEY 244
AL+ FYA WCGHCK L P Y
Sbjct: 42 ALIEFYATWCGHCKSLAPVY 61
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 36.7 bits (81), Expect = 0.004
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 149 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEY 244
S+F +++ Q +V F+A WCG CK + P++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +2
Query: 152 DFSAVLSQHDTALVMFYAPWCGHCKRLKP 238
D++ +S +V FYA WCG CK LKP
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP 55
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 33.5 bits (73), Expect = 0.036
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +2
Query: 140 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP 238
LTD+D + +S+ T + +Y P CG CKRL P
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGP 62
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = +1
Query: 289 LAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 435
+A ++C K C+Q+S+ +PT +F K E EY G +V +
Sbjct: 333 VAHINCAVS-KRACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPBC32C12.03c |ppk25||serine/threonine protein kinase Ppk25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 29.9 bits (64), Expect = 0.44
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Frame = -3
Query: 558 LLSGQIPFRRIRQPLLHLRSKSFKI-------SHCKELLGAGSNLGTH---VLDNAIRFP 409
++ G++PF + P+LH + KS K+ C L+ A N+ L+ A +FP
Sbjct: 244 MVCGRVPFDDVSVPMLHSKIKSGKLEFPSYISEDCCSLIAAMLNVNPRKRCSLEQAAKFP 303
Query: 408 W 406
W
Sbjct: 304 W 304
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 29.5 bits (63), Expect = 0.58
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 543 SDLKGEFLKTADKLREEVTFAHSSANEVLEKNRIQKQCGSC 665
S+L L +D L+ + +H+S VL + +K C SC
Sbjct: 221 SNLSSNNLFQSDSLKPSMVSSHTSTKPVLYRGNSEKSCHSC 261
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 28.3 bits (60), Expect = 1.3
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 6/87 (6%)
Frame = +3
Query: 537 KESDLKGEFLKTADKLREEVTFAHSSANEVLEKNR-IQKQCGS---CIVPSDSRTS--LK 698
+E D F K +K+R + +S E +EK R + K+C S + S RT+ L+
Sbjct: 1042 REKDSALSFKKDYEKIRSDADRVITSLKEDIEKERSLMKECHSNYESEIVSHGRTTQKLR 1101
Query: 699 TLPVAFDGDTEKVFTPKLSSKGTYHGL 779
L FD K K + + + GL
Sbjct: 1102 DLRTEFDEVNTKYLKLKANFEQQHSGL 1128
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 27.9 bits (59), Expect = 1.8
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 200 YAPWCGHCKRLKPEYAVAAA 259
YA WCG CK + P ++ A+
Sbjct: 27 YADWCGPCKAISPLFSQLAS 46
>SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 480
Score = 27.5 bits (58), Expect = 2.3
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 717 RKQPEESSNLFWSRLGRYKTHIV 649
+ PEE SN+ WS + + KT +V
Sbjct: 192 KDDPEEDSNMAWSIIHKIKTEVV 214
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 4.1
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +3
Query: 132 FSILQIPTFRLFYLNMIQPWSCF 200
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPBC28E12.01c |apc13|SPBC9B6.12c|anaphase-promoting complex subunit
Apc13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 135
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 588 EEVTFAHSSANEVLEKNR 641
+E+T HSSAN +L KN+
Sbjct: 45 DEMTIQHSSANLILMKNK 62
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.4 bits (53), Expect = 9.5
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = +2
Query: 188 LVMFYAPWCGHCKRL 232
L+ FYAPW CK++
Sbjct: 24 LLNFYAPWAAPCKQM 38
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = -1
Query: 452 PTWARMYLTMPLDSLGPLYSEESSPFLKIFS 360
P ++Y+T+ D +G E +SP +K+ S
Sbjct: 1414 PQKEKLYITISADEVGKFILEATSPTVKVSS 1444
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,363,457
Number of Sequences: 5004
Number of extensions: 69869
Number of successful extensions: 210
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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