Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30869
         (757 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   204   8e-54
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   197   1e-51
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   100   3e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    94   2e-20
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||...    27   2.9  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   3.8  
SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S...    27   3.8  
SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At...    26   5.0  
SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual      26   6.7  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   6.7  
SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Sc...    26   6.7  
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy...    25   8.8  
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |...    25   8.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  204 bits (499), Expect = 8e-54
 Identities = 92/173 (53%), Positives = 120/173 (69%)
 Frame = +2

Query: 2   ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 181
           IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK+VPR
Sbjct: 5   ISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPR 64

Query: 182 AVFVDLEPTVVDEVRTGTYRQLFHQNNLLLVRKMRXXXXXXXXXXLAKEIVDLVLDRIRK 361
           +++VDLEP V+D+VRTG YR LFH   L+  ++            + KE+VD V D+IR+
Sbjct: 65  SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124

Query: 362 LADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGNQSKLEFAI*PAPHV 520
           +AD C+GLQGFL+FH            LL+ERL+++Y  +SKL+F++ PAP V
Sbjct: 125 IADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  197 bits (481), Expect = 1e-51
 Identities = 94/178 (52%), Positives = 122/178 (68%), Gaps = 5/178 (2%)
 Frame = +2

Query: 2   ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAG 166
           ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSETG G
Sbjct: 5   ISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQG 63

Query: 167 KHVPRAVFVDLEPTVVDEVRTGTYRQLFHQNNLLLVRKMRXXXXXXXXXXLAKEIVDLVL 346
           K VPR+++VDLEP V+D+VRTG Y+ LFH   ++  ++            + KE++D VL
Sbjct: 64  KFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVL 123

Query: 347 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGNQSKLEFAI*PAPHV 520
           +RIR++AD C+GLQGFL+FH            LL+ERL+++YG +S L+F++ PAP V
Sbjct: 124 ERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  100 bits (239), Expect = 3e-22
 Identities = 53/171 (30%), Positives = 82/171 (47%)
 Frame = +2

Query: 2   ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 181
           + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK+VPR
Sbjct: 5   VHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPR 62

Query: 182 AVFVDLEPTVVDEVRTGTYRQLFHQNNLLLVRKMRXXXXXXXXXXLAKEIVDLVLDRIRK 361
           AV VDLEP  +D V++G +  LF  +N++  +                E+ D VLD +R+
Sbjct: 63  AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122

Query: 362 LADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGNQSKLEFAI*PAP 514
            A+ C  LQGF + H            LL+ ++  +Y ++    F++ PAP
Sbjct: 123 EAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAP 173


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 93.9 bits (223), Expect = 2e-20
 Identities = 52/170 (30%), Positives = 87/170 (51%), Gaps = 1/170 (0%)
 Frame = +2

Query: 2   ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 181
           I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   +++PR
Sbjct: 6   ITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPR 63

Query: 182 AVFVDLEPTVVDEVRTGTYRQLFHQNNLLLVRKMRXXXXXXXXXXL-AKEIVDLVLDRIR 358
           A+ +DLEP VV+ + + TY  L++  N+L+ +               A+ I + ++D I 
Sbjct: 64  AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMDMID 123

Query: 359 KLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGNQSKLEFAI*P 508
           + AD    L+GF + H             L+ERL+  Y  +    +++ P
Sbjct: 124 READGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173


>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 3971

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 24/66 (36%), Positives = 34/66 (51%), Gaps = 4/66 (6%)
 Frame = +3

Query: 348  TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTATSLNWSSP--SSPRL 515
            T S +  TS P+   +   SSTP      L S       +P+T++T+LN S+P  SS  L
Sbjct: 3037 TSSTALNTSTPITSSTVLNSSTPITSSSVLNSS------TPITSSTALNTSTPITSSSVL 3090

Query: 516  MFSTAV 533
              STA+
Sbjct: 3091 NSSTAI 3096



 Score = 26.2 bits (55), Expect = 5.0
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
 Frame = +3

Query: 348 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTATSLNWSSP--SSPRL 515
           T S +  TS P+   S   SSTP      L S       +P+T++T+LN S+P  SS  L
Sbjct: 625 TSSTALNTSTPITSSSVLNSSTPITSSSVLNSS------TPITSSTALNTSTPITSSSVL 678

Query: 516 MFSTAV 533
             ST +
Sbjct: 679 NSSTPI 684



 Score = 26.2 bits (55), Expect = 5.0
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
 Frame = +3

Query: 348  TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTATSLNWSSP--SSPRL 515
            T S +  TS P+   S   SSTP      L S       +P+T++T+LN S+P  SS  L
Sbjct: 1873 TSSTALNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSSVL 1926

Query: 516  MFSTAV 533
              ST +
Sbjct: 1927 NSSTPI 1932



 Score = 26.2 bits (55), Expect = 5.0
 Identities = 24/66 (36%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
 Frame = +3

Query: 348  TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTATSLNWSSP--SSPRL 515
            T S +  TS P+   S   SSTP      L S       +P+T++T+LN S+P  SS  L
Sbjct: 2065 TSSTALNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSSVL 2118

Query: 516  MFSTAV 533
              ST +
Sbjct: 2119 NSSTPI 2124



 Score = 25.8 bits (54), Expect = 6.7
 Identities = 24/66 (36%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
 Frame = +3

Query: 348  TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTATSLNWSSP--SSPRL 515
            T S    TS P+   S   SSTP      L S       +P+T++T+LN S+P  SS  L
Sbjct: 937  TSSTGLNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSSVL 990

Query: 516  MFSTAV 533
              ST +
Sbjct: 991  NSSTPI 996


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 44/170 (25%), Positives = 67/170 (39%), Gaps = 2/170 (1%)
 Frame = +3

Query: 69  STASSLMARCPQTRPSGVETILSTLSSARP-ELASTYPVXXXXXXXXXXXXXXXXAHTDS 245
           ST SSL +    ++PS   T  ST SSA P   +S+Y                  +   S
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTS 232

Query: 246 CFIRTTYYW*GRCGQQLCPWSLHHWQRKS*IWFWTESASSLTSVPVCKDS*SSTPSVEVP 425
             + T+              SL      S     T S+SS +S  +   S SS+      
Sbjct: 233 SSLSTSSIPSTSSSSSSTSSSLSSSSSSS-----TASSSSSSSSIISSSSSSSSSPTSTS 287

Query: 426 ALGSLPY*WSVSPLTTATSLNWSSPSSPRLMFSTAVVEPYNSYAF-SSPT 572
           +  S     S SP +T+++++ SS SS     + +     +S +F SSPT
Sbjct: 288 STISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPT 337


>SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein
           Pkl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 9/38 (23%), Positives = 21/38 (55%)
 Frame = +2

Query: 608 WVRQLKGHSIDILDRR*FSIIGVAQTYDQPRDRLHWDK 721
           WV +L+G  ++I +   + ++  A+    P+  +H D+
Sbjct: 611 WVYKLRGQFLEIYNETIYDLLNKAEMLKNPKHDIHHDE 648


>SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
 Frame = +1

Query: 520 FPLRSSSPTT-LMPSAHPHNPLSTLNCSFSLGT 615
           FP +  + T+ + P AHPHN L + N  F + T
Sbjct: 510 FPGKVQTLTSGISPVAHPHNSLGSSNEIFDIDT 542


>SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 656

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 487 TGVRHLARASCFPLRSSSPTTLMPS 561
           TG +HL  +SC  +  S  T  MP+
Sbjct: 167 TGFKHLRHSSCLEMAGSDRTPSMPA 191


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 88  WPDAHRQDHRGWRRFFQHFLQR 153
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor
           Fep1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 564

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 16/36 (44%), Positives = 22/36 (61%)
 Frame = +1

Query: 487 TGVRHLARASCFPLRSSSPTTLMPSAHPHNPLSTLN 594
           TGV  L  AS FP  +S+ T  +PS+   +PL TL+
Sbjct: 283 TGV--LPPASTFPSYNSNFTGFLPSSFNPSPLMTLS 316


>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2052

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 8/29 (27%), Positives = 14/29 (48%)
 Frame = -1

Query: 373  LVSELADSVQNQIYDFLCQWCSDHGHSCW 287
            L S+++    NQI + +  WC     + W
Sbjct: 1857 LYSQMSSDENNQILELIAGWCEHLAQNTW 1885


>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 638

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 14/42 (33%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +3

Query: 459 SPLTTATSLN-WSSPSSPRLMFSTAVVEPYNSYAFSSPTQPF 581
           SPL   TS++ +S+P    +   ++  +P +SY  S P+ PF
Sbjct: 146 SPLLANTSISSFSAPIDANINSYSSFSDPASSYKPSLPSVPF 187


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,321,522
Number of Sequences: 5004
Number of extensions: 71336
Number of successful extensions: 281
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 266
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)