Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30868
(850 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p... 55 7e-08
At5g26070.1 68418.m03102 hydroxyproline-rich glycoprotein family... 32 0.55
At5g60350.1 68418.m07566 hypothetical protein 31 1.3
At4g28150.2 68417.m04037 expressed protein 31 1.3
At4g28150.1 68417.m04036 expressed protein 31 1.3
At2g35340.1 68415.m04333 RNA helicase, putative similar to ATP-d... 29 3.9
At1g17830.1 68414.m02207 expressed protein 29 3.9
At5g65640.1 68418.m08257 basic helix-loop-helix (bHLH) family pr... 29 5.2
At5g51300.2 68418.m06360 splicing factor-related contains simila... 29 5.2
At5g51300.1 68418.m06359 splicing factor-related contains simila... 29 5.2
At3g26750.1 68416.m03346 expressed protein 29 5.2
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 28 9.0
At2g20300.1 68415.m02371 protein kinase family protein contains ... 28 9.0
>At2g26300.1 68415.m03156 guanine nucleotide binding protein
(G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
identical to SP|P18064 Guanine nucleotide-binding
protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
thaliana}
Length = 383
Score = 54.8 bits (126), Expect = 7e-08
Identities = 25/65 (38%), Positives = 45/65 (69%)
Frame = +3
Query: 186 QIDR*ELEREWYSGIKRHQAIVVGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSN 365
+I+R +E+E + + +++GAGESGKSTI KQ+K++ ++GF + K Y PV+++N
Sbjct: 23 EIER-RIEQEAKAEKHIRKLLLLGAGESGKSTIFKQIKLLFQTGFDEGELKSYVPVIHAN 81
Query: 366 TIQSL 380
Q++
Sbjct: 82 VYQTI 86
>At5g26070.1 68418.m03102 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965; similar to root nodule extensin
[Pisum sativum] gi|15021750|gb|AAK77902; Common family
members: At5g19800, At5g57070, At1g72790 [Arabidopsis
thaliana]
Length = 102
Score = 31.9 bits (69), Expect = 0.55
Identities = 20/61 (32%), Positives = 27/61 (44%), Gaps = 2/61 (3%)
Frame = -1
Query: 193 SICFEQAAARSSADWAHPISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVA--PALPE 20
+I F A A ++ + P+ P +P P L P H Y+RPVA P LP
Sbjct: 15 AILFTVAEANNNRKLLKTPTNYQPLYSPSPSPYRSPVTLPPPPPHPAYSRPVALPPTLPI 74
Query: 19 P 17
P
Sbjct: 75 P 75
>At5g60350.1 68418.m07566 hypothetical protein
Length = 292
Score = 30.7 bits (66), Expect = 1.3
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = -2
Query: 777 RNRCPPTFGTIWKLKVFGRRNVTSTNSK 694
RNRC P+FG+I++ K G++ + +K
Sbjct: 10 RNRCLPSFGSIYRSKRTGKKEAAAAKTK 37
>At4g28150.2 68417.m04037 expressed protein
Length = 283
Score = 30.7 bits (66), Expect = 1.3
Identities = 19/64 (29%), Positives = 33/64 (51%)
Frame = -1
Query: 349 GRYCLKSSFVNPLSWIIFICLTIVLLPDSPAPTTIA*CLLMPEYHSLSSSYRSICFEQAA 170
G L+S ++P SW+ I +P + + ++ C L YH+LSSS++ + E+
Sbjct: 170 GLRSLRSVDLSPASWMAVAWYPIYHIPMNRSIKDLSTCFLT--YHTLSSSFQDVKREEEK 227
Query: 169 ARSS 158
R S
Sbjct: 228 ERIS 231
>At4g28150.1 68417.m04036 expressed protein
Length = 285
Score = 30.7 bits (66), Expect = 1.3
Identities = 19/64 (29%), Positives = 33/64 (51%)
Frame = -1
Query: 349 GRYCLKSSFVNPLSWIIFICLTIVLLPDSPAPTTIA*CLLMPEYHSLSSSYRSICFEQAA 170
G L+S ++P SW+ I +P + + ++ C L YH+LSSS++ + E+
Sbjct: 172 GLRSLRSVDLSPASWMAVAWYPIYHIPMNRSIKDLSTCFLT--YHTLSSSFQDVKREEEK 229
Query: 169 ARSS 158
R S
Sbjct: 230 ERIS 233
>At2g35340.1 68415.m04333 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1110
Score = 29.1 bits (62), Expect = 3.9
Identities = 15/35 (42%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = +3
Query: 222 SGIKRHQA-IVVGAGESGKSTIVKQMKIIHESGFT 323
+ +K HQ I+VG SGK+T + Q +HE+G+T
Sbjct: 481 NAVKDHQVLIIVGETGSGKTTQIPQY--LHEAGYT 513
>At1g17830.1 68414.m02207 expressed protein
Length = 337
Score = 29.1 bits (62), Expect = 3.9
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = -1
Query: 349 GRYCLKSSFVNPLSWIIFICLTIVLLPDSPAPTTIA*CLLMPEYHSLSSSYRSICFE 179
G L+S ++P SW+ I +P ++ C L YH+LSS+++ E
Sbjct: 199 GLSTLRSVDLSPASWLAIAWYPIYHIPSRKTDKDLSTCFL--SYHTLSSAFQGNLIE 253
>At5g65640.1 68418.m08257 basic helix-loop-helix (bHLH) family
protein
Length = 351
Score = 28.7 bits (61), Expect = 5.2
Identities = 24/72 (33%), Positives = 29/72 (40%)
Frame = -1
Query: 811 LERQWNPISSFSESLPAHVRNNLEIEGFWKEKCYFDEFQVVLTSKSFRIILFGGAGNPLL 632
LE I S S S P ++N E E F Y SKSF + GG N
Sbjct: 110 LEALQEIIDSSSSSPPLILQNGQE-ENFNNPMSYPSPLMESDQSKSFSVGYCGGETNKKK 168
Query: 631 PKPVRGRPGKYL 596
K + G+P K L
Sbjct: 169 SKKLEGQPSKNL 180
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.7 bits (61), Expect = 5.2
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 142 PISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 14
P +P P A P P PG+ P+ H + P P PP
Sbjct: 604 PWGPPVPSYSPYALPPPPPGSYHPV--HGQHMPPYGMQYPPPP 644
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.7 bits (61), Expect = 5.2
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 142 PISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 14
P +P P A P P PG+ P+ H + P P PP
Sbjct: 604 PWGPPVPSYSPYALPPPPPGSYHPV--HGQHMPPYGMQYPPPP 644
>At3g26750.1 68416.m03346 expressed protein
Length = 526
Score = 28.7 bits (61), Expect = 5.2
Identities = 18/66 (27%), Positives = 33/66 (50%)
Frame = +3
Query: 621 TGLGSKGLPAPPNKIILKDLEVKTTWNSSK*HFSFQKPSISKLFRTWAGSDSEKEEIGFH 800
+G+GS G P + K + T+ + + F+K ++ ++F + KEE+ FH
Sbjct: 367 SGVGSNGKPIDGGVRLFK-CYISTSSTTGESSDVFRKYTLERMFTNQL-VECSKEELSFH 424
Query: 801 CLSRDL 818
L +DL
Sbjct: 425 VLVKDL 430
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 27.9 bits (59), Expect = 9.0
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = -1
Query: 124 PMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 14
P ++P +P P P +S +Y+ P P EPP
Sbjct: 583 PPIYPYLSPPPPPTPVSSPPPTPVYSPPPPPPCIEPP 619
>At2g20300.1 68415.m02371 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 744
Score = 27.9 bits (59), Expect = 9.0
Identities = 23/58 (39%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = -1
Query: 235 LLMPEYHSLSSSYRSICFEQAAARS---SADWAHPISAVLPMLWPQAAPTPEPGALSP 71
LL+ HSL+S ICF + S + AH + P L P APTP P A SP
Sbjct: 9 LLILLLHSLASF--PICFARLFPMSLPFTRSKAHQMHFFHPYLNPSVAPTPSP-AFSP 63
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,140,210
Number of Sequences: 28952
Number of extensions: 467726
Number of successful extensions: 1717
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1715
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1970388800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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