BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30862 (895 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g08840.1 68414.m00984 DNA replication helicase, putative simi... 42 6e-04 At2g47350.1 68415.m05911 PAPA-1-like family protein / zinc finge... 32 0.59 At2g07734.1 68415.m00983 ribosomal protein S4 (RPS4) identical t... 31 0.78 At4g05633.1 68417.m00876 hypothetical protein 30 1.8 At3g57550.1 68416.m06408 guanylate kinase 2 (GK-2) identical to ... 29 4.2 At5g60270.1 68418.m07554 lectin protein kinase family protein co... 28 9.6 At4g27910.1 68417.m04006 PHD finger protein-related / SET domain... 28 9.6 At3g23350.1 68416.m02945 expressed protein ; expression supporte... 28 9.6 >At1g08840.1 68414.m00984 DNA replication helicase, putative similar to helicase [Xenopus laevis] gi|18845092|gb|AAL79550 Length = 1296 Score = 41.9 bits (94), Expect = 6e-04 Identities = 27/71 (38%), Positives = 42/71 (59%), Gaps = 4/71 (5%) Frame = +3 Query: 6 IDATLQVTVP-EKNVLQKAIVPLELKSGKA---SVSAEHRGQLVMYGMMLSLHRNEDPTV 173 IDA+++V V + N + + I+PLE KSGKA S EH Q+++Y +++S E Sbjct: 537 IDASVRVIVESDMNTVNEKIMPLEFKSGKAPSGQSSIEHSAQVILYTLLMS----ERYLK 592 Query: 174 ALQRGLLLYLR 206 + GLL YL+ Sbjct: 593 HIDNGLLYYLQ 603 >At2g47350.1 68415.m05911 PAPA-1-like family protein / zinc finger (HIT type) family protein contains Pfam domains, PF04795: PAPA-1-like conserved region and PF04438: HIT zinc finger Length = 486 Score = 31.9 bits (69), Expect = 0.59 Identities = 27/74 (36%), Positives = 36/74 (48%), Gaps = 1/74 (1%) Frame = -3 Query: 803 PNGLTVEYWTIANSPS*-GPFPSNLI*PPERRNT*RCFSPDCSHCL*FSDWELQVPLFSL 627 PNG TV + I PS P PS PP R N C P C++ + D + +VPL S Sbjct: 419 PNGTTVSF-PIDKVPSLFDPKPSGY--PPPREN---CVGPSCTNPYKYRDSKTKVPLCS- 471 Query: 626 FSALSVHKACKGDE 585 L +KA +G + Sbjct: 472 ---LKCYKAVQGQQ 482 >At2g07734.1 68415.m00983 ribosomal protein S4 (RPS4) identical to small ribosomal protein 4 [Arabidopsis thaliana] GI:1460051 Length = 362 Score = 31.5 bits (68), Expect = 0.78 Identities = 32/115 (27%), Positives = 50/115 (43%), Gaps = 8/115 (6%) Frame = +2 Query: 314 DPEQLTDIEDASFLLQQKLPE---PINHERACTKCPYLSICSVHLWHSGGPSVSENHPLS 484 +PE D+ + +P+ PI+H R C +SI L H S EN+ + Sbjct: 102 NPETRFDVIPLRLHFLETIPQARQPISHRRVCVNKGMVSITHFKLSHGDIISFQENNAII 161 Query: 485 KLQGQALGTYPKIT*NISFIGRLY---LR-WKRNFKRIHHLYK-PCGLRVLKKEK 634 + + Y +I+ IG+L LR W+R+ HL K G R+L K + Sbjct: 162 RGEEIRRSFYKEISVE-KIIGKLLHQPLRMWRRSKTEWFHLLKTKRGCRLLLKSR 215 >At4g05633.1 68417.m00876 hypothetical protein Length = 376 Score = 30.3 bits (65), Expect = 1.8 Identities = 17/41 (41%), Positives = 25/41 (60%), Gaps = 2/41 (4%) Frame = +2 Query: 584 IHHLYKPCGLRVLKKEK--KEGLVIPNLKIKDSGSNRVKNI 700 + HLY+ L ++KEK K GL+ P+ K SN+VKN+ Sbjct: 240 VQHLYQSLSLNSIEKEKTFKIGLLDPHRK--SESSNQVKNV 278 >At3g57550.1 68416.m06408 guanylate kinase 2 (GK-2) identical to guanylate kinase (GK-2) [Arabidopsis thaliana] gi|7861798|gb|AAF70409 Length = 389 Score = 29.1 bits (62), Expect = 4.2 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = +1 Query: 169 LWLYNVDCCFI*ETK*T*GRLTVDTQRGALGNAEESV 279 +W VD FI E K GR V +G GNAE+ + Sbjct: 104 IWFLEVDTPFIREQKKLLGREVVAWSKGVRGNAEKPI 140 >At5g60270.1 68418.m07554 lectin protein kinase family protein contains Pfam domains, PF00069: Protein kinase domain, PF00139: Legume lectins beta domain, and PF00138: Legume lectins alpha domain Length = 668 Score = 27.9 bits (59), Expect = 9.6 Identities = 13/62 (20%), Positives = 28/62 (45%) Frame = +3 Query: 54 KAIVPLELKSGKASVSAEHRGQLVMYGMMLSLHRNEDPTVALQRGLLLYLRDKVDVREVN 233 + ++ ++K+ + AE G+L +GM R DP+ G + Y+ ++ + Sbjct: 462 QVVIHRDIKAANVMLDAEFNGRLGDFGMSRLYDRGADPSTTAAVGTVGYMAPELTTMGAS 521 Query: 234 CG 239 G Sbjct: 522 TG 523 >At4g27910.1 68417.m04006 PHD finger protein-related / SET domain-containing protein (TX4) nearly identical over 285 amino acids to trithorax 4 [Arabidopsis thaliana] GI:16118405; contains Pfam profiles PF00856: SET domain, PF00855: PWWP domain; identical to cDNA trithorax 4 (TX4) partial cds GI:16118404 Length = 1027 Score = 27.9 bits (59), Expect = 9.6 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = -3 Query: 683 CSHCL*FSDWELQVPLFSLFSALSVHKACKGDEFV*NFSSILSKAVQ 543 C+ C DW+ + ++VH+ C G V +F+S + KA + Sbjct: 595 CAVCRWVEDWDYNKIIICNRCQIAVHQECYGARHVRDFTSWVCKACE 641 >At3g23350.1 68416.m02945 expressed protein ; expression supported by MPSS Length = 225 Score = 27.9 bits (59), Expect = 9.6 Identities = 13/26 (50%), Positives = 18/26 (69%), Gaps = 1/26 (3%) Frame = +2 Query: 278 LVENLTS-DPKDPDPEQLTDIEDASF 352 LVE +T+ DP PD + +T I +ASF Sbjct: 37 LVEEVTNGDPSSPDAKTMTKIAEASF 62 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,217,482 Number of Sequences: 28952 Number of extensions: 439359 Number of successful extensions: 1182 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1181 length of database: 12,070,560 effective HSP length: 81 effective length of database: 9,725,448 effective search space used: 2100696768 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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