Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30857
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 32 0.094
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr... 31 0.29
SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr 1|||M... 29 0.88
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 28 2.0
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 27 3.5
SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.7
SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa... 26 6.2
SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual 26 8.2
SPAC15A10.13 |ppk3||serine/threonine protein kinase Ppk3|Schizos... 26 8.2
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 8.2
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 32.3 bits (70), Expect = 0.094
Identities = 21/60 (35%), Positives = 29/60 (48%)
Frame = +3
Query: 375 LAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDKS*ASHPRKICLTVLK 554
L + + QG+LVR LK + A + DI K VA+L K + S S +K LK
Sbjct: 5 LVDINERITSQGNLVRSLKSQGASKEDIDKEVAKLLQLKNLKLGGSEVSGKKKDTSFTLK 64
>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 652
Score = 30.7 bits (66), Expect = 0.29
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3
Query: 567 LKGAFFYDQSFAIYGGLLGQFDF 635
+K FF+ SF IYGG+ G +D+
Sbjct: 19 MKKRFFFSPSFQIYGGISGLYDY 41
Score = 29.5 bits (63), Expect = 0.66
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +2
Query: 539 FDRAKMEDLIKRRFFL*PVFCYIWWITG 622
FDR + E+L+K+RFF P F I+G
Sbjct: 10 FDRTQFEELMKKRFFFSPSFQIYGGISG 37
Score = 27.1 bits (57), Expect = 3.5
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +1
Query: 634 FGPMGCALKSNMIPLYGKKFFI 699
+GP G AL+SN++ ++ K F I
Sbjct: 41 YGPPGSALQSNLVDIWRKHFVI 62
>SPAC458.02c |||mRNP complex |Schizosaccharomyces pombe|chr
1|||Manual
Length = 468
Score = 29.1 bits (62), Expect = 0.88
Identities = 20/69 (28%), Positives = 40/69 (57%)
Frame = +3
Query: 288 WGSNKLHRKIKIPNPFREIIMADPKIEEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKA 467
+G+ K + +P PF + D +E++ + + A+ KEQ D V K + EKA + +I+K
Sbjct: 375 FGTIKEFDFVGVPAPFTKS-QVDSAVEQLKSRI-AHFKEQQDSVTKQRIEKAKQ-EIEKL 431
Query: 468 VAELKTRKK 494
A+ ++++
Sbjct: 432 EAKYNSKEE 440
>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 683
Score = 27.9 bits (59), Expect = 2.0
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +3
Query: 306 HRKIKIPNPFREIIMADPKIEEILAPLRANVKEQGDLVRKLK 431
HRK+ I + R+II+ D + E + PL+ DL +LK
Sbjct: 626 HRKVVISSTARKIIVLDKERERVELPLQEASAFSEDLRSRLK 667
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = -1
Query: 418 TKSPCSFTLALKGARISSILG-SAIMISRK 332
T SPCSFT+ AR+ S+ G S + + R+
Sbjct: 60 TLSPCSFTICSLRARLHSLHGPSTVFLKRE 89
>SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 167
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 339 EIIMADPKIEEILAPLRANVKEQGDLVRKLKEEK 440
E+ A+P+ E +LA + +KEQ + + KE+K
Sbjct: 121 EVACANPEDEALLAERKQRLKEQREKKEQKKEQK 154
>SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate
transaminase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 696
Score = 26.2 bits (55), Expect = 6.2
Identities = 14/27 (51%), Positives = 15/27 (55%), Gaps = 1/27 (3%)
Frame = -1
Query: 880 FPRGAL*LKGS-KPESTHPGFKNPWVN 803
FP A L G+ KP S HP F NP N
Sbjct: 216 FPEPAEGLPGTPKPTSLHPVFSNPATN 242
>SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 656
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 681 IKWNHVTLKCTSHRTKNQTGPVIHHI*QKTGHR 583
I +NHV + T T N G V + Q++GHR
Sbjct: 513 ISYNHVRVPPTRIFTINSAGEVHIELLQRSGHR 545
>SPAC15A10.13 |ppk3||serine/threonine protein kinase
Ppk3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 637
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/48 (27%), Positives = 21/48 (43%)
Frame = +3
Query: 366 EEILAPLRANVKEQGDLVRKLKEEKAPEIDIKKAVAELKTRKKILEDK 509
E + AP + N + +P ID+KK ELK +++K
Sbjct: 537 ENVSAPAKFNGSFWSKFIHSSSASPSPSIDMKKESLELKNDTTEIKEK 584
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -1
Query: 673 ESCYS*VH-IP*DQKSNWPSNPPYIAKDWS*KKAP 572
E C +H IP S + +P Y+ +DW+ K AP
Sbjct: 613 EYCKQGIHMIPTTPISGYLRSPSYVQQDWNAKIAP 647
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,379,731
Number of Sequences: 5004
Number of extensions: 68026
Number of successful extensions: 156
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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