Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30852
(759 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 207 1e-54
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 201 8e-53
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 89 6e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 77 4e-15
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 2.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.7
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 6.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 207 bits (506), Expect = 1e-54
Identities = 99/172 (57%), Positives = 125/172 (72%), Gaps = 3/172 (1%)
Frame = +2
Query: 5 ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 184
IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR
Sbjct: 5 ISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPR 64
Query: 185 AC---LRRS*TYCC**GPHWHIQTVVYPEQLITGKEDAANNYARGHYTIGKEIVDLVLDR 355
+ L + GP+ + + +PEQLITGKEDA+NNYARGHYT+GKE+VD V D+
Sbjct: 65 SIYVDLEPNVIDQVRTGPY---RDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDK 121
Query: 356 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 511
IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YP
Sbjct: 122 IRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173
Score = 98.3 bits (234), Expect = 1e-21
Identities = 50/79 (63%), Positives = 63/79 (79%), Gaps = 1/79 (1%)
Frame = +1
Query: 508 PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPNLQP-N*IVS 684
PAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+ + N +++
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIA 232
Query: 685 SDRLSPSINWLSLRFERAL 741
++ SI SLRFE +L
Sbjct: 233 --QVVSSIT-ASLRFEGSL 248
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 201 bits (491), Expect = 8e-53
Identities = 101/177 (57%), Positives = 127/177 (71%), Gaps = 8/177 (4%)
Frame = +2
Query: 5 ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAG 169
ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG G
Sbjct: 5 ISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQG 63
Query: 170 KHVPRAC---LRRS*TYCC**GPHWHIQTVVYPEQLITGKEDAANNYARGHYTIGKEIVD 340
K VPR+ L + GP+ + + +PEQ++TGKEDA+NNYARGHYT+GKE++D
Sbjct: 64 KFVPRSIYVDLEPNVIDQVRTGPY---KDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 341 LVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 511
VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YP
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177
Score = 100 bits (239), Expect = 3e-22
Identities = 43/53 (81%), Positives = 48/53 (90%)
Frame = +1
Query: 508 PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPNLQ 666
PAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERP +
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYE 229
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 89.0 bits (211), Expect = 6e-19
Identities = 51/169 (30%), Positives = 77/169 (45%)
Frame = +2
Query: 5 ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 184
+ + GQ G Q+G A W EHG+ G T + N +F+E GK+VPR
Sbjct: 5 VHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPR 62
Query: 185 ACLRRS*TYCC**GPHWHIQTVVYPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 364
A L + P+ +I G+ A N +A+GHYT G E+ D VLD +R+
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122
Query: 365 LADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 511
A+ C LQGF + H LL+ ++ +Y + F++ P
Sbjct: 123 EAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAP 171
Score = 52.8 bits (121), Expect = 5e-08
Identities = 24/51 (47%), Positives = 33/51 (64%)
Frame = +1
Query: 508 PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPN 660
PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPS 221
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 76.6 bits (180), Expect = 4e-15
Identities = 52/171 (30%), Positives = 83/171 (48%), Gaps = 2/171 (1%)
Frame = +2
Query: 5 ISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPR 184
I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PR
Sbjct: 6 ITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPR 63
Query: 185 ACLRRS*TYCC**GPHWHIQTVVYPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRI 358
A L ++ PE ++ K A NN+A G Y+ + I + ++D I
Sbjct: 64 AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122
Query: 359 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 511
+ AD L+GF + H L+ERL+ Y KK ++++P
Sbjct: 123 DREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173
Score = 35.9 bits (79), Expect = 0.006
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 520 VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPNL-QPN*IVSS 687
VS VV+PYNS+L ++D ++DN A+ I L + P Q N +VS+
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVST 234
Score = 31.5 bits (68), Expect = 0.13
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 180 PVHVFVDLEPTVVDEVRTGTYRQLFIQNNLLLVR 281
P + +DLEP VV+ + + TY L+ N+L+ +
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITK 95
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 27.5 bits (58), Expect = 2.2
Identities = 18/68 (26%), Positives = 37/68 (54%), Gaps = 4/68 (5%)
Frame = +1
Query: 523 STAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI----ERPNLQPN*IVSSD 690
S+++V+ + SI + + SD F +D+ I+D+ ++DI + PN N ++++
Sbjct: 157 SSSLVDNF-SISSVPSVFFQSD--FNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNS 213
Query: 691 RLSPSINW 714
L I+W
Sbjct: 214 MLQEKISW 221
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 72 STASSLMARCPQTRPSGVETILSTLSSARPELAST 176
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 91 WPDAHRQDHRGWRRFFQHFLQR 156
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.8 bits (54), Expect = 6.7
Identities = 20/58 (34%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +3
Query: 351 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTRAS 518
T S + TS P+ S SSTP L S +P+T++ +LN S+P T +S
Sbjct: 625 TSSTALNTSTPITSSSVLNSSTPITSSSVLNSS------TPITSSTALNTSTPITSSS 676
Score = 25.8 bits (54), Expect = 6.7
Identities = 20/58 (34%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +3
Query: 351 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTRAS 518
T S + TS P+ S SSTP L S +P+T++ +LN S+P T +S
Sbjct: 1873 TSSTALNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSS 1924
Score = 25.8 bits (54), Expect = 6.7
Identities = 20/58 (34%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +3
Query: 351 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTRAS 518
T S + TS P+ S SSTP L S +P+T++ +LN S+P T +S
Sbjct: 2065 TSSTALNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSS 2116
Score = 25.4 bits (53), Expect = 8.9
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 2/58 (3%)
Frame = +3
Query: 351 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTRAS 518
T S TS P+ S SSTP L S +P+T++ +LN S+P T +S
Sbjct: 937 TSSTGLNTSTPITSSSVLNSSTPITSSTVLNSS------TPITSSTALNTSTPITSSS 988
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,166,158
Number of Sequences: 5004
Number of extensions: 64824
Number of successful extensions: 244
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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