SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30831
         (725 letters)

Database: celegans 
           27,780 sequences; 12,740,198 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z81122-5|CAB03357.1|  579|Caenorhabditis elegans Hypothetical pr...    32   0.48 
AF022974-3|AAC48037.1|  346|Caenorhabditis elegans Seven tm rece...    31   0.84 
Z99270-1|CAB16470.4|  500|Caenorhabditis elegans Hypothetical pr...    31   1.1  
U41023-1|AAA82340.1|  569|Caenorhabditis elegans Hypothetical pr...    31   1.1  
AL021497-3|CAA16405.1|  613|Caenorhabditis elegans Hypothetical ...    30   1.5  
AF039046-2|AAB94218.2|  516|Caenorhabditis elegans Hypothetical ...    30   1.9  
Z81508-6|CAB04147.1|  338|Caenorhabditis elegans Hypothetical pr...    29   2.6  
AF040646-6|AAK31540.1|  510|Caenorhabditis elegans Hypothetical ...    29   2.6  
AF040646-5|AAK31539.1|  492|Caenorhabditis elegans Hypothetical ...    29   2.6  
U64846-9|AAG24117.3|  343|Caenorhabditis elegans Serpentine rece...    29   3.4  
AF047661-5|AAU05544.1|  822|Caenorhabditis elegans Hypothetical ...    29   3.4  
AF039042-11|AAC48251.1|  343|Caenorhabditis elegans Serpentine r...    29   3.4  
AC084159-8|AAK39361.2|  655|Caenorhabditis elegans Hypothetical ...    29   3.4  
Z82263-6|CAJ76936.1|  383|Caenorhabditis elegans Hypothetical pr...    29   4.5  
Z82263-3|CAB62793.2|  451|Caenorhabditis elegans Hypothetical pr...    29   4.5  
Z82263-2|CAE46670.1|  445|Caenorhabditis elegans Hypothetical pr...    29   4.5  
Z81579-5|CAB04654.3| 1305|Caenorhabditis elegans Hypothetical pr...    28   5.9  
Z81058-5|CAB02921.1|  274|Caenorhabditis elegans Hypothetical pr...    28   5.9  
Z68160-4|CAA92292.1|  583|Caenorhabditis elegans Hypothetical pr...    28   5.9  
Z66522-7|CAA91409.2|  472|Caenorhabditis elegans Hypothetical pr...    28   5.9  
Z66520-13|CAA91392.2|  472|Caenorhabditis elegans Hypothetical p...    28   5.9  
AY959881-1|AAX56914.1| 1305|Caenorhabditis elegans nephrocystin-...    28   5.9  
Z83246-1|CAB05842.2|  312|Caenorhabditis elegans Hypothetical pr...    28   7.8  
Z78418-4|CAB01698.1|  710|Caenorhabditis elegans Hypothetical pr...    28   7.8  
AL031627-21|CAA20970.1|  326|Caenorhabditis elegans Hypothetical...    28   7.8  

>Z81122-5|CAB03357.1|  579|Caenorhabditis elegans Hypothetical
           protein T13F2.6 protein.
          Length = 579

 Score = 31.9 bits (69), Expect = 0.48
 Identities = 18/52 (34%), Positives = 26/52 (50%)
 Frame = +3

Query: 27  SHQQKSDVDYSRGFHGTTVLW*RCSCSVFTIYIQLYHRAYYTAKWNLRRQNP 182
           SH  +  VD S  FH   +     S  ++ I +  +HR+   AKW L R+NP
Sbjct: 370 SHDVEFVVDISGMFHDQNL-----SLGLYEIKVVRFHRSNVFAKWRLARKNP 416


>AF022974-3|AAC48037.1|  346|Caenorhabditis elegans Seven tm
           receptor protein 207 protein.
          Length = 346

 Score = 31.1 bits (67), Expect = 0.84
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = +3

Query: 345 YFYDTNKQAVQNVSWILLVALIVYIVCYAIGLSTVPY 455
           YFY+TN   + ++ +   +A+IV I+   + L TV Y
Sbjct: 184 YFYETNSDGITDIYYPSFIAIIVNILITIVSLITVFY 220


>Z99270-1|CAB16470.4|  500|Caenorhabditis elegans Hypothetical
           protein Y37A1A.3 protein.
          Length = 500

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 20/97 (20%), Positives = 43/97 (44%)
 Frame = +3

Query: 330 IGTYFYFYDTNKQAVQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQ 509
           +G  + F +T  Q V   S+ L+ A+ ++   +A+G   +     GE+ P N +  + + 
Sbjct: 375 VGLMYTFAETQNQIV---SYFLISAICMFNFLFAMGPGPLSMFITGELVPQNCRSASSVW 431

Query: 510 AHIYTGVAMFAVQNLFRVVEQAYRIYTVFWGFTIFLI 620
            +    V  F +   +  V+    + + F  + IF +
Sbjct: 432 TNAVMAVVRFLILTFYLPVK---NMTSEFMAYAIFFV 465


>U41023-1|AAA82340.1|  569|Caenorhabditis elegans Hypothetical
           protein F53H8.3 protein.
          Length = 569

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
 Frame = +1

Query: 1   GSWSDLFTEATNKKAMWITLGVFTVQQFCGSAAVVAYSQFIF-NCTTGPTILPNGTL 168
           G++ +LFT    +  + +++ V   QQF G  AV A+S  +F N    P I    TL
Sbjct: 309 GTFRELFTRPDLRIPLAVSIIVMIAQQFTGCTAVFAFSTDMFLNAGLTPVIARFSTL 365


>AL021497-3|CAA16405.1|  613|Caenorhabditis elegans Hypothetical
           protein Y51A2D.5 protein.
          Length = 613

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 16/75 (21%), Positives = 34/75 (45%)
 Frame = +3

Query: 396 LVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQNLFRVVEQA 575
           +V + VY++ ++ G +++P+V   E +P   +      + +   V    +   +  +  A
Sbjct: 476 IVMMGVYLLTFSCGFTSLPWVLNSEFYPMWARSTCVSISTLSNWVFNLIIALTYLSLTHA 535

Query: 576 YRIYTVFWGFTIFLI 620
              Y  FW + IF I
Sbjct: 536 ITKYGAFWLYAIFTI 550


>AF039046-2|AAB94218.2|  516|Caenorhabditis elegans Hypothetical
           protein R09B5.11 protein.
          Length = 516

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 22/101 (21%), Positives = 43/101 (42%)
 Frame = +3

Query: 384 SWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQNLFRV 563
           S++ ++ ++ ++V +A G   +P+    E+F +  +  A   +     VA + V   F  
Sbjct: 405 SYMNVLFILSFVVTFAFGPGPIPWFFTSELFDSATRGRAAAVSATSNWVANWMVGLTFLP 464

Query: 564 VEQAYRIYTVFWGFTIFLISWTRLSWVNDAYQKTKRESSFR 686
           +      Y  F  FT F  ++   +W      K K  S+ R
Sbjct: 465 INNIIHQY-AFLMFTFFTFTFAIFTWKFVPETKGKSPSAIR 504


>Z81508-6|CAB04147.1|  338|Caenorhabditis elegans Hypothetical
           protein F20E11.10 protein.
          Length = 338

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 24/96 (25%), Positives = 46/96 (47%), Gaps = 7/96 (7%)
 Frame = +3

Query: 330 IGTYFYFYDTNKQAVQNVSWILL-VALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFL 506
           +G Y   Y+T +Q ++ V W LL + + V +  Y++ L T+P++   ++   ++ L  +L
Sbjct: 35  LGLYCVLYETPRQ-MKGVKWYLLNLHVSVMLFDYSVTLLTIPFLLATKLAGFSLGLAKYL 93

Query: 507 QAHI----YTGVAMFAVQNLFRVV--EQAYRIYTVF 596
                    T V  F +  +  VV  E  +R+   F
Sbjct: 94  SVPFIVPALTAVYCFGLMFIAIVVIFENRFRVICTF 129


>AF040646-6|AAK31540.1|  510|Caenorhabditis elegans Hypothetical
           protein H17B01.1b protein.
          Length = 510

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 17/77 (22%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
 Frame = +3

Query: 405 LIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQNLFRVVEQAYRI 584
           ++++++ +A G   +P+  + E+F ++ +  A   A +    A   V   F  +    + 
Sbjct: 411 VLLFVISFATGPGAIPWFFVSEIFDSSARGNANSIAVMVNWAANLLVGLTFLPINNLMQQ 470

Query: 585 YT--VFWGFTIFLISWT 629
           Y+  +F GF  F I +T
Sbjct: 471 YSFFIFSGFLAFFIFYT 487


>AF040646-5|AAK31539.1|  492|Caenorhabditis elegans Hypothetical
           protein H17B01.1a protein.
          Length = 492

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 17/77 (22%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
 Frame = +3

Query: 405 LIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQNLFRVVEQAYRI 584
           ++++++ +A G   +P+  + E+F ++ +  A   A +    A   V   F  +    + 
Sbjct: 393 VLLFVISFATGPGAIPWFFVSEIFDSSARGNANSIAVMVNWAANLLVGLTFLPINNLMQQ 452

Query: 585 YT--VFWGFTIFLISWT 629
           Y+  +F GF  F I +T
Sbjct: 453 YSFFIFSGFLAFFIFYT 469


>U64846-9|AAG24117.3|  343|Caenorhabditis elegans Serpentine
           receptor, class h protein193 protein.
          Length = 343

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 23/76 (30%), Positives = 39/76 (51%), Gaps = 4/76 (5%)
 Frame = +3

Query: 330 IGTYFYFYDTNKQAVQNVSWILLVALIVYIVC--YAIGLSTVPYVTI--GEMFPTNVKLY 497
           +G Y   Y T ++ ++ V W L  AL  +IV   Y++ L TVP++ I  G  +P  V  Y
Sbjct: 35  LGLYCIIYKTPER-MKTVKWYLF-ALHSWIVVFDYSLSLLTVPFLLIPKGAGYPLGVSQY 92

Query: 498 AFLQAHIYTGVAMFAV 545
             +   +Y  + M ++
Sbjct: 93  TDVPL-VYQTIIMMSL 107


>AF047661-5|AAU05544.1|  822|Caenorhabditis elegans Hypothetical
           protein M70.4 protein.
          Length = 822

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = -1

Query: 623 RNEKNSEPPEHCVNSISLFD 564
           R+E +S+PP  C+N I LFD
Sbjct: 86  RSETDSQPPFACLNDIKLFD 105


>AF039042-11|AAC48251.1|  343|Caenorhabditis elegans Serpentine
           receptor, class h protein199 protein.
          Length = 343

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +3

Query: 330 IGTYFYFYDTNKQAVQNVSWILLVALI-VYIVCYAIGLSTVPYV 458
           +G Y   + T KQ +++V W +L+  I V I  Y+  + TVP+V
Sbjct: 35  LGLYCIIFKTPKQ-MRSVKWYMLILHIYVMIFDYSYTIMTVPFV 77


>AC084159-8|AAK39361.2|  655|Caenorhabditis elegans Hypothetical
           protein Y73B3A.3 protein.
          Length = 655

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 11/20 (55%), Positives = 15/20 (75%)
 Frame = -1

Query: 623 RNEKNSEPPEHCVNSISLFD 564
           R+E +S+PP  C+N I LFD
Sbjct: 6   RSETDSQPPFACLNDIKLFD 25


>Z82263-6|CAJ76936.1|  383|Caenorhabditis elegans Hypothetical
           protein C47A4.2c protein.
          Length = 383

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 20/83 (24%), Positives = 35/83 (42%)
 Frame = +3

Query: 372 VQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQN 551
           ++ + +  L  + V++V Y + LS  P V  G  F      Y      +Y G+A+F   N
Sbjct: 210 LETIKYCWLQCICVFLV-YFVSLSVFPTVLAG--FQPG---YTVFPNDVYAGIAVFLNFN 263

Query: 552 LFRVVEQAYRIYTVFWGFTIFLI 620
            F  V      +  F G  + ++
Sbjct: 264 FFAAVGNVAATFVTFPGPRLLIV 286


>Z82263-3|CAB62793.2|  451|Caenorhabditis elegans Hypothetical
           protein C47A4.2b protein.
          Length = 451

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 20/83 (24%), Positives = 35/83 (42%)
 Frame = +3

Query: 372 VQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQN 551
           ++ + +  L  + V++V Y + LS  P V  G  F      Y      +Y G+A+F   N
Sbjct: 278 LETIKYCWLQCICVFLV-YFVSLSVFPTVLAG--FQPG---YTVFPNDVYAGIAVFLNFN 331

Query: 552 LFRVVEQAYRIYTVFWGFTIFLI 620
            F  V      +  F G  + ++
Sbjct: 332 FFAAVGNVAATFVTFPGPRLLIV 354


>Z82263-2|CAE46670.1|  445|Caenorhabditis elegans Hypothetical
           protein C47A4.2a protein.
          Length = 445

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 20/83 (24%), Positives = 35/83 (42%)
 Frame = +3

Query: 372 VQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYTGVAMFAVQN 551
           ++ + +  L  + V++V Y + LS  P V  G  F      Y      +Y G+A+F   N
Sbjct: 272 LETIKYCWLQCICVFLV-YFVSLSVFPTVLAG--FQPG---YTVFPNDVYAGIAVFLNFN 325

Query: 552 LFRVVEQAYRIYTVFWGFTIFLI 620
            F  V      +  F G  + ++
Sbjct: 326 FFAAVGNVAATFVTFPGPRLLIV 348


>Z81579-5|CAB04654.3| 1305|Caenorhabditis elegans Hypothetical protein
            R13H4.1 protein.
          Length = 1305

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
 Frame = +2

Query: 230  RLLPVSC--PSPRGPSRTQTASLAIDFGRRTNEWNNRNVFLLLRYEQASRT 376
            R+L   C  PS R   R  T    + F   + E N+R  FLLL Y    +T
Sbjct: 1046 RVLSCRCSDPSVRLTVRNATLQQIVGFTTYSGETNDRKTFLLLMYSDHYQT 1096


>Z81058-5|CAB02921.1|  274|Caenorhabditis elegans Hypothetical
           protein F11E6.5 protein.
          Length = 274

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 19/52 (36%), Positives = 31/52 (59%), Gaps = 2/52 (3%)
 Frame = +2

Query: 488 QAVRVPASTHIYRRR-YVCSAESLPGCRTSL*NLHSVLGVHYFSH-FLDSSF 637
           Q V+   S +I+    ++ SA+S+PGC  S  N+ S+ G+ Y S+ FL + F
Sbjct: 206 QIVQFVISCYIFGHLVFIKSADSVPGCAVSW-NVLSIGGLMYISYLFLFAKF 256


>Z68160-4|CAA92292.1|  583|Caenorhabditis elegans Hypothetical
           protein D1046.4 protein.
          Length = 583

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 21/96 (21%), Positives = 43/96 (44%), Gaps = 2/96 (2%)
 Frame = +3

Query: 345 YFYDTNKQAVQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIYT 524
           Y YD  +Q+V+  +W + + +IV+++        +  +       TN+    FLQ   + 
Sbjct: 322 YLYDIMEQSVEKTAWTVSLIIIVHLILKIF----ISRIFEKNPLLTNIYSIKFLQFSSFA 377

Query: 525 GVAMFAVQNLFRVVEQAYRIYTVFWG--FTIFLISW 626
           G A+  +  LF V +  + I  + +   F    ++W
Sbjct: 378 GSAVIMI-TLF-VTKFHFDIQLILYAAIFAFLSLTW 411


>Z66522-7|CAA91409.2|  472|Caenorhabditis elegans Hypothetical
           protein F14E5.1 protein.
          Length = 472

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 25/102 (24%), Positives = 48/102 (47%)
 Frame = +3

Query: 342 FYFYDTNKQAVQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIY 521
           F F+   +  +  + +  LVALI+Y   Y  G+  + +    E+ P   K  +  Q+  Y
Sbjct: 352 FVFFAKIRILIDPMKYGCLVALIIYGYTYGTGVGPISWFISSELVPQ--KHRSIAQSVAY 409

Query: 522 TGVAMFAVQNLFRVVEQAYRIYTVFWGFTIFLISWTRLSWVN 647
           +   +  V + F V+     +Y+V  G   FLI ++  S+++
Sbjct: 410 SMNTVMVVISTFTVLP----LYSVI-GSYAFLILYSIPSFIS 446


>Z66520-13|CAA91392.2|  472|Caenorhabditis elegans Hypothetical
           protein F14E5.1 protein.
          Length = 472

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 25/102 (24%), Positives = 48/102 (47%)
 Frame = +3

Query: 342 FYFYDTNKQAVQNVSWILLVALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIY 521
           F F+   +  +  + +  LVALI+Y   Y  G+  + +    E+ P   K  +  Q+  Y
Sbjct: 352 FVFFAKIRILIDPMKYGCLVALIIYGYTYGTGVGPISWFISSELVPQ--KHRSIAQSVAY 409

Query: 522 TGVAMFAVQNLFRVVEQAYRIYTVFWGFTIFLISWTRLSWVN 647
           +   +  V + F V+     +Y+V  G   FLI ++  S+++
Sbjct: 410 SMNTVMVVISTFTVLP----LYSVI-GSYAFLILYSIPSFIS 446


>AY959881-1|AAX56914.1| 1305|Caenorhabditis elegans
            nephrocystin-4-like protein protein.
          Length = 1305

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
 Frame = +2

Query: 230  RLLPVSC--PSPRGPSRTQTASLAIDFGRRTNEWNNRNVFLLLRYEQASRT 376
            R+L   C  PS R   R  T    + F   + E N+R  FLLL Y    +T
Sbjct: 1046 RVLSCRCSDPSVRLTVRNATLQQIVGFTTYSGETNDRKTFLLLMYSDHYQT 1096


>Z83246-1|CAB05842.2|  312|Caenorhabditis elegans Hypothetical
           protein ZK795.1 protein.
          Length = 312

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 11/30 (36%), Positives = 17/30 (56%)
 Frame = +1

Query: 103 VAYSQFIFNCTTGPTILPNGTLEDKTRKSI 192
           VA  + + +CTT P+  P  T  D+ RK +
Sbjct: 60  VALYELLRSCTTSPSTPPESTTSDELRKRV 89


>Z78418-4|CAB01698.1|  710|Caenorhabditis elegans Hypothetical
           protein F25D7.5 protein.
          Length = 710

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 13/51 (25%), Positives = 25/51 (49%)
 Frame = +1

Query: 82  FCGSAAVVAYSQFIFNCTTGPTILPNGTLEDKTRKSIEPYQESIIIGCVQV 234
           F G+ +++   QF F C+T P   P   +    +  +E Y  + ++ CV +
Sbjct: 295 FFGNVSILKNFQFFFRCSTKPNKKPQ-KIPAMIQWMLEGYNANQVVRCVHL 344


>AL031627-21|CAA20970.1|  326|Caenorhabditis elegans Hypothetical
           protein Y102A5C.33 protein.
          Length = 326

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 2/87 (2%)
 Frame = +3

Query: 345 YFYDTNKQAVQNVSWILL-VALIVYIVCYAIGLSTVPYVTIGEMFPTNVKLYAFLQAHIY 521
           YF +  KQ    +  I L V L++YI+   I     P++ I  +       +   Q HIY
Sbjct: 168 YFLNFGKQLGDEIMNITLSVYLVLYIIVNVIICFLTPFIYIPIIIGMKKHRHLHSQQHIY 227

Query: 522 TGVAMFAVQNLFRVVEQAYRIYTV-FW 599
               MF +Q++  ++   ++I T  FW
Sbjct: 228 IHEYMF-IQSIMVLL---FKIVTAPFW 250


  Database: celegans
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 12,740,198
  Number of sequences in database:  27,780
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,924,865
Number of Sequences: 27780
Number of extensions: 395774
Number of successful extensions: 1185
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 1113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1185
length of database: 12,740,198
effective HSP length: 79
effective length of database: 10,545,578
effective search space used: 1708383636
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -