Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30829
(810 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 68 1e-12
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 65 1e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 61 2e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 1e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 40 4e-04
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 38 0.002
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.016
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.26
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.2
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 3.2
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 5.5
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 68.1 bits (159), Expect = 1e-12
Identities = 28/62 (45%), Positives = 46/62 (74%)
Frame = +2
Query: 308 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 487
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +FV +
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQM 137
Query: 488 IM 493
I+
Sbjct: 138 IL 139
Score = 46.8 bits (106), Expect = 4e-06
Identities = 27/62 (43%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +3
Query: 93 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFCQ-FT 269
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E F Q
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 270 RP 275
RP
Sbjct: 66 RP 67
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +3
Query: 90 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 203
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 64.9 bits (151), Expect = 1e-11
Identities = 30/71 (42%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +2
Query: 281 KARSADTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQG 457
K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G
Sbjct: 77 KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136
Query: 458 NINYENFVHLI 490
INYE F +I
Sbjct: 137 VINYEEFSRVI 147
Score = 48.8 bits (111), Expect = 9e-07
Identities = 20/45 (44%), Positives = 34/45 (75%)
Frame = +3
Query: 72 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 206
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQ 51
Score = 33.9 bits (74), Expect = 0.027
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +2
Query: 308 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 484
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 485 LI 490
++
Sbjct: 73 MM 74
Score = 33.5 bits (73), Expect = 0.036
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +3
Query: 78 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 203
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 61.3 bits (142), Expect = 2e-10
Identities = 28/73 (38%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = +2
Query: 278 SKARSADTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQ 454
+K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q +
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNS 128
Query: 455 GNINYENFVHLIM 493
G+ +Y +FV IM
Sbjct: 129 GSFDYYDFVQRIM 141
Score = 41.9 bits (94), Expect = 1e-04
Identities = 20/51 (39%), Positives = 32/51 (62%)
Frame = +3
Query: 72 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHL 224
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 35.1 bits (77), Expect = 0.012
Identities = 15/56 (26%), Positives = 30/56 (53%)
Frame = +3
Query: 90 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVF 257
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYY 134
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 41.5 bits (93), Expect = 1e-04
Identities = 22/62 (35%), Positives = 33/62 (53%)
Frame = +2
Query: 305 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 484
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE FV
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEKFVD 175
Query: 485 LI 490
I
Sbjct: 176 AI 177
Score = 33.9 bits (74), Expect = 0.027
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +3
Query: 63 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKP 230
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINP 95
Score = 29.9 bits (64), Expect = 0.45
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 317 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 436
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
Score = 29.5 bits (63), Expect = 0.59
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 99 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 206
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 39.9 bits (89), Expect = 4e-04
Identities = 20/46 (43%), Positives = 29/46 (63%)
Frame = +3
Query: 72 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKK 209
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLK 77
Score = 30.7 bits (66), Expect = 0.26
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +2
Query: 308 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QEDSQGNINYENFVH 484
D E + FD D + I ELR + LG EV ++L+ + +G + E+FV
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 485 LIMQ 496
++ +
Sbjct: 98 VMTE 101
Score = 29.5 bits (63), Expect = 0.59
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +3
Query: 78 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 206
D L E + AF+LFD GKI + + + L +N + +++
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Score = 25.4 bits (53), Expect = 9.6
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = +2
Query: 305 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYEN 475
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ N
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRN 131
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 37.5 bits (83), Expect = 0.002
Identities = 23/68 (33%), Positives = 35/68 (51%)
Frame = -3
Query: 424 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVGTACFRYGLVNWQNTSK 245
L+L+ L++ +Q + R + IAIF K + +K I SV R+ L Q K
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQKLPK 1436
Query: 244 DILSSGLR 221
++L SGLR
Sbjct: 1437 ELLQSGLR 1444
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.016
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +2
Query: 326 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 496
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.26
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +2
Query: 332 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 487
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = +3
Query: 90 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFCQ 263
E +EAF LFD G I + + LG+N T+ ++ + ++S E F +
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAE 70
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.2
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +2
Query: 356 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 499
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.1 bits (57), Expect = 3.2
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +2
Query: 326 RHFDKDGNGFISS-AELRHLLSTLGEK-LSDDEVEQLL 433
RH DK GN F ++ EL +L +G+K L++ +E +L
Sbjct: 670 RHIDKSGNEFTTAYLELLEVLLKVGQKYLAESLLEHIL 707
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 26.2 bits (55), Expect = 5.5
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = -3
Query: 496 LHDEVNKVLIVDISLRVFLSLQELLYLIITELLSESREQVSQFRRRDEP 350
+HD+V+K S+ VF L LLY + E L +QVS ++R P
Sbjct: 735 MHDDVDKEQFGYSSMYVFFRLFNLLYERLYE-LQRLEDQVSIIQQRIIP 782
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,212,198
Number of Sequences: 5004
Number of extensions: 63776
Number of successful extensions: 215
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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