Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30812
(749 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27170.1 68415.m06029 structural maintenance of chromosomes (... 32 0.47
At2g47230.1 68415.m05898 agenet domain-containing protein contai... 31 0.62
At4g26760.1 68417.m03855 microtubule associated protein (MAP65/A... 31 1.1
At5g46460.1 68418.m05722 pentatricopeptide (PPR) repeat-containi... 30 1.4
At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almos... 30 1.4
At5g24630.1 68418.m02909 expressed protein ; expression support... 30 1.4
At5g50230.1 68418.m06221 transducin family protein / WD-40 repea... 29 2.5
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 29 3.3
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 29 3.3
At3g05310.1 68416.m00579 GTP-binding protein-related low similar... 29 3.3
At2g37080.1 68415.m04550 myosin heavy chain-related low similari... 29 4.4
At4g12770.1 68417.m02004 auxilin-related low similarity to SP|Q2... 28 5.8
At3g18480.1 68416.m02348 CCAAT displacement protein-related / CD... 28 5.8
At1g65070.1 68414.m07377 DNA mismatch repair MutS family protein... 28 5.8
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 28 5.8
At5g40820.1 68418.m04956 FAT domain-containing protein / phospha... 28 7.6
At1g53800.1 68414.m06123 expressed protein 28 7.6
At1g03780.2 68414.m00358 targeting protein-related similar to mi... 28 7.6
At1g03780.1 68414.m00359 targeting protein-related similar to mi... 28 7.6
>At2g27170.1 68415.m06029 structural maintenance of chromosomes
(SMC) family protein similar to basement
membrane-associated chondroitin proteoglycan Bamacan
[Rattus norvegicus] GI:1785540; contains Pfam profile
PF02463: RecF/RecN/SMC N terminal domain. No suitalble
start codon was identified.
Length = 1207
Score = 31.9 bits (69), Expect = 0.47
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = +3
Query: 252 GGIRRGRDLLEQGHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANEIYKTL 416
G I+ D LEQ + + E+ D L +L+ ++ ++ V+ T NE+ KTL
Sbjct: 318 GNIQSKNDALEQLNTVEREMQDSLRELEAIKPLYESQVDKENQTSKRINELEKTL 372
>At2g47230.1 68415.m05898 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 701
Score = 31.5 bits (68), Expect = 0.62
Identities = 24/87 (27%), Positives = 43/87 (49%), Gaps = 1/87 (1%)
Frame = +3
Query: 225 ERRHPSQFRGGIRRGRDLLE-QGHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANE 401
ER ++ R G+ + + E +GH +EE+ +L K+ EL+ Q E+ +AT +
Sbjct: 615 ERAKKAEERKGLEKKIEAGEIEGHTYEEEMAELELKILELKRQQVVAKEMKEATDKVTSG 674
Query: 402 IYKTLKEFSDQVTESHKQAIQATMDVP 482
+ K+ E +Q E + Q+T P
Sbjct: 675 M-KSYAEMINQEIEDLRLEFQSTASAP 700
>At4g26760.1 68417.m03855 microtubule associated protein
(MAP65/ASE1) family protein low similarity to protein
regulating cytokinesis 1 (PRC1) [Homo sapiens]
GI:2865521; contains Pfam profile PF03999: Microtubule
associated protein (MAP65/ASE1 family)
Length = 578
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/76 (22%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +3
Query: 243 QFRGGIRRGRDLLEQGHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANEIYKTLK- 419
+ GG+ G ++++ + L+D KL ELQ + + ++ ++ +++ L+
Sbjct: 138 EIAGGLNNGPHVVDETDLSLKRLDDFQRKLQELQKEKSDRLQKVLEFVSTVHDLCAVLRL 197
Query: 420 EFSDQVTESHKQAIQA 467
+F VTE H +A
Sbjct: 198 DFLSTVTEVHPSLDEA 213
>At5g46460.1 68418.m05722 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 697
Score = 30.3 bits (65), Expect = 1.4
Identities = 25/78 (32%), Positives = 35/78 (44%), Gaps = 1/78 (1%)
Frame = +1
Query: 70 ARAVYDEALGLYAEVNTTLLPDIKLNKLHEDSMEMNRTIDEKSAELDRLIAENEDTLHNL 249
+R V+DE + V T LL LNK HED++ + S L I N+ T +
Sbjct: 279 SRKVFDEKVHEQVAVWTALLSGYSLNKKHEDALSI------FSGMLRNSILPNQSTFASG 332
Query: 250 EEA*GAVGTC-WNKVTTG 300
+ A+GT W K G
Sbjct: 333 LNSCSALGTLDWGKEMHG 350
>At5g41790.1 68418.m05088 COP1-interactive protein 1 / CIP1 almost
identical to CIP1 (GI:836950) [Arabidopsis thaliana]
Length = 1305
Score = 30.3 bits (65), Expect = 1.4
Identities = 24/83 (28%), Positives = 36/83 (43%)
Frame = +1
Query: 4 ELNAASGKLNSMTELADQALKRARAVYDEALGLYAEVNTTLLPDIKLNKLHEDSMEMNRT 183
E+N S K+ EL + L + R+ DE L E N + K+N + M +
Sbjct: 929 EINGLSEKIKGR-ELELETLGKQRSELDEELRTKKEENVQMHD--KINVASSEIMALTEL 985
Query: 184 IDEKSAELDRLIAENEDTLHNLE 252
I+ ELD L + +T LE
Sbjct: 986 INNLKNELDSLQVQKSETEAELE 1008
>At5g24630.1 68418.m02909 expressed protein ; expression supported
by MPSS
Length = 531
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +2
Query: 509 EESINSITEELVAASDKAKEGQGTWRTGRKRNMPIKLLRPPT-KSRRKRP-LPKAK 670
++S+ + E A KAK +G G+KR P K +PP K+R P PKAK
Sbjct: 476 DQSVGTEEETNTKAKPKAK-AKGETVIGKKRGRPSKEKQPPAKKARNSAPKKPKAK 530
>At5g50230.1 68418.m06221 transducin family protein / WD-40 repeat
family protein contains 7 WD-40 repeats (PF00400);
similar to TIPD PROTEIN (SP:O15736)[Dictyostelium
discoideum]
Length = 515
Score = 29.5 bits (63), Expect = 2.5
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 139 KLNKLHEDSMEMNRTIDEKSAELDRLIAENEDTLHNLEE 255
+L + ED + ++EK+ +D LIAEN + LEE
Sbjct: 105 ELTQRREDCTRLQEELEEKTKTVDVLIAENLEIRSQLEE 143
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/55 (29%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
Frame = +1
Query: 91 ALGLYAEVNTTLLPDIKLNKLHEDSMEM-NRTIDEKSAELDRLIAENEDTLHNLE 252
A G+ E+ L + ++ + HE E+ +R I+EK E+ RL+ E + ++E
Sbjct: 559 AEGIEKELENAKLRNKRMKEEHESVRELADRLIEEKDREISRLVDEMTNLRKSME 613
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/55 (29%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
Frame = +1
Query: 91 ALGLYAEVNTTLLPDIKLNKLHEDSMEM-NRTIDEKSAELDRLIAENEDTLHNLE 252
A G+ E+ L + ++ + HE E+ +R I+EK E+ RL+ E + ++E
Sbjct: 559 AEGIEKELENAKLRNKRMKEEHESVRELADRLIEEKDREISRLVDEMTNLRKSME 613
>At3g05310.1 68416.m00579 GTP-binding protein-related low similarity
to rac 1 protein [Physcomitrella patens] GI:7243743;
contains Pfam profile PF00036: EF hand (domain)
Length = 648
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/55 (27%), Positives = 21/55 (38%)
Frame = +3
Query: 288 GHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANEIYKTLKEFSDQVTESHK 452
G+ L D L + QA VELT + E+Y+ D E H+
Sbjct: 286 GYSNDLRLGDDLVPYSSFKRQADQSVELTNVAIEFLREVYEFFDSNGDNNLEPHE 340
>At2g37080.1 68415.m04550 myosin heavy chain-related low similarity
to myosin heavy chain [Rana catesbeiana] GI:4249701
Length = 583
Score = 28.7 bits (61), Expect = 4.4
Identities = 19/52 (36%), Positives = 28/52 (53%)
Frame = +1
Query: 7 LNAASGKLNSMTELADQALKRARAVYDEALGLYAEVNTTLLPDIKLNKLHED 162
++ A KL S+TE AD++ KRA E LG NT L +++ K+ D
Sbjct: 455 IDEALAKLGSLTEEADKSGKRAENA-TEQLGAAQVTNTELEAELRRLKVQCD 505
>At4g12770.1 68417.m02004 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 909
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = +3
Query: 225 ERRHPSQFRGGIRRGRDLLEQGHDRQEELNDLLAKLDELQAQAKNDVELTKA 380
ERR + R D E R+ EL + +LD +A+ + ++E T+A
Sbjct: 474 ERREKESLKASRSREGDHTENYDSRERELREKQVRLDRERAEREAEMEKTQA 525
>At3g18480.1 68416.m02348 CCAAT displacement protein-related /
CDP-related similar to CCAAT displacement protein (CDP)
(Cut-like 1) (Swiss-Prot:P39880) [Homo sapiens];
contains Pfam:PF00904 Involucrin repeat
Length = 689
Score = 28.3 bits (60), Expect = 5.8
Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = +3
Query: 270 RDLLEQGHDRQEELNDLLAKLDELQAQAKNDVE-LTKATLTAANEIYKTLKEFSDQVTES 446
R+L E+ E L D L + QAK+ V + K A N++++ L+ SD+ T
Sbjct: 195 RNLAEENQKTMELLKDREQALQDQLRQAKDSVSTMQKLHELAQNQLFE-LRAQSDEETAG 253
Query: 447 HKQAIQATMD 476
+ + MD
Sbjct: 254 KQSEVSLLMD 263
>At1g65070.1 68414.m07377 DNA mismatch repair MutS family protein
contains Pfam profile PF00488: MutS domain V
Length = 857
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/41 (34%), Positives = 28/41 (68%), Gaps = 1/41 (2%)
Frame = +3
Query: 261 RRGRDLLEQGHDR-QEELNDLLAKLDELQAQAKNDVELTKA 380
+R R LL++ + QE+LN +K++ L A+ ++ +E+T+A
Sbjct: 635 KRERALLKKETQKVQEDLNSAKSKMERLVAEFESQLEITQA 675
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 28.3 bits (60), Expect = 5.8
Identities = 24/85 (28%), Positives = 35/85 (41%)
Frame = +1
Query: 1 NELNAASGKLNSMTELADQALKRARAVYDEALGLYAEVNTTLLPDIKLNKLHEDSMEMNR 180
NEL +L M + A+ L+ +A Y+ L +E KL+ M
Sbjct: 653 NELRMQKRQLEEMIKDANDELRANQAEYEAKLHELSE---------KLSFKTSQMERMLE 703
Query: 181 TIDEKSAELDRLIAENEDTLHNLEE 255
+DEKS E+D ED NL +
Sbjct: 704 NLDEKSNEIDNQKRHEEDVTANLNQ 728
>At5g40820.1 68418.m04956 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
similar to Atr protein [Xenopus laevis] GI:11385422;
contains Pfam profiles PF00454 Phosphatidylinositol 3-
and 4-kinase, PF02259 FAT domain, PF02260 FAT C domain
Length = 2702
Score = 27.9 bits (59), Expect = 7.6
Identities = 20/68 (29%), Positives = 32/68 (47%)
Frame = -1
Query: 479 HVHRGLDRLLVRLGHLITELFQSLVNLICSCQRGFREFHVVFSLRLQLIQLSEEIVELLL 300
HV G LL H+ ELF +L + S REF L ++ +L ++V +L
Sbjct: 912 HVKGGFATLLSTASHIQNELFDNLSVRLTSRPNVVREFAEAV-LGVETEELVRKMVPAVL 970
Query: 299 PVVTLFQQ 276
P + ++ Q
Sbjct: 971 PKLLVYWQ 978
>At1g53800.1 68414.m06123 expressed protein
Length = 568
Score = 27.9 bits (59), Expect = 7.6
Identities = 23/83 (27%), Positives = 34/83 (40%), Gaps = 1/83 (1%)
Frame = +2
Query: 434 SDRVAQASDPSHDGRAGCPREGSGGEESINSITEELVAASDKAKEGQGTWRTGRKRN-MP 610
S ++ AS S +G A GEE ++ + KA G W GRK +
Sbjct: 79 SSSLSSASSKSSNGSA------DDGEEQVDDREKLRRMRISKANRGNTPWNKGRKHSPET 132
Query: 611 IKLLRPPTKSRRKRPLPKAKTVN 679
++ +R TK + P K K N
Sbjct: 133 LQKIRERTKIAMQDPKIKMKLAN 155
>At1g03780.2 68414.m00358 targeting protein-related similar to
microtubule-associated protein / targeting protein for
Xklp2 ((TPX2) GI:8926138) {Homo sapiens}; similar to
Restricted expression proliferation associated protein
100 (p100) (Differentially expressed in lung cells 2)
(DIL-2) (Targeting protein for Xklp2) (C20orf1 protein)
(C20orf2 protein) (Protein FLS353)(SP:Q9ULW0) {Homo
sapiens}
Length = 725
Score = 27.9 bits (59), Expect = 7.6
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +3
Query: 261 RRGRDLLEQGHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANEIYKTLKEFSDQVT 440
+R R L + EE ++LAK+ + +A+ N E T+ AN+ +T S +V+
Sbjct: 329 QRARPLRVKSSAELEE--EMLAKIPKFKARPVNKKEFHLQTMARANQHAETSSIASTEVS 386
Query: 441 ESH 449
+ H
Sbjct: 387 KQH 389
>At1g03780.1 68414.m00359 targeting protein-related similar to
microtubule-associated protein / targeting protein for
Xklp2 ((TPX2) GI:8926138) {Homo sapiens}; similar to
Restricted expression proliferation associated protein
100 (p100) (Differentially expressed in lung cells 2)
(DIL-2) (Targeting protein for Xklp2) (C20orf1 protein)
(C20orf2 protein) (Protein FLS353)(SP:Q9ULW0) {Homo
sapiens}
Length = 687
Score = 27.9 bits (59), Expect = 7.6
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +3
Query: 261 RRGRDLLEQGHDRQEELNDLLAKLDELQAQAKNDVELTKATLTAANEIYKTLKEFSDQVT 440
+R R L + EE ++LAK+ + +A+ N E T+ AN+ +T S +V+
Sbjct: 329 QRARPLRVKSSAELEE--EMLAKIPKFKARPVNKKEFHLQTMARANQHAETSSIASTEVS 386
Query: 441 ESH 449
+ H
Sbjct: 387 KQH 389
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,404,109
Number of Sequences: 28952
Number of extensions: 229924
Number of successful extensions: 969
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 930
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 969
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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