Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30800
(743 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 131 5e-31
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 130 1e-30
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 126 2e-29
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 44 8e-05
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 44 8e-05
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 42 6e-04
At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17 si... 29 2.5
At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17 si... 29 2.5
At1g40129.1 68414.m04766 hypothetical protein 29 2.5
At2g40770.1 68415.m05030 SNF2 domain-containing protein / helica... 29 4.3
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 29 4.3
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 5.7
At1g01120.1 68414.m00015 fatty acid elongase 3-ketoacyl-CoA synt... 28 5.7
At1g17970.1 68414.m02223 zinc finger (C3HC4-type RING finger) fa... 28 7.5
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 27 9.9
At3g10470.1 68416.m01255 zinc finger (C2H2 type) family protein ... 27 9.9
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 131 bits (316), Expect = 5e-31
Identities = 57/86 (66%), Positives = 71/86 (82%)
Frame = +3
Query: 249 SYPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGM 428
S PEG +VCN+E +GDRG LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M
Sbjct: 106 SIPEGAVVCNVEHHVGDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAM 165
Query: 429 VGIVAGGGRIDKPILKAGRAYHKYKV 506
+G VAGGGR +KP+LKAG AYHKY+V
Sbjct: 166 IGQVAGGGRTEKPMLKAGNAYHKYRV 191
Score = 93.1 bits (221), Expect = 2e-19
Identities = 41/59 (69%), Positives = 49/59 (83%)
Frame = +2
Query: 2 RKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 178
RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 23 RKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHPFRFKKQKELFVAAEG 81
Score = 74.5 bits (175), Expect = 7e-14
Identities = 30/36 (83%), Positives = 33/36 (91%)
Frame = +2
Query: 506 QRNCWPYVRGVAMNPVEHPHGGGNHQHIGKASTVKK 613
+RN WP VRGVAMNPVEHPHGGGNHQHIG ASTV++
Sbjct: 192 KRNSWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRR 227
Score = 44.0 bits (99), Expect = 1e-04
Identities = 17/26 (65%), Positives = 23/26 (88%)
Frame = +1
Query: 178 LYTGQFVYCGKXSTLEVGNVMPVGAI 255
+YTGQF+YCGK +TL VGNV+P+ +I
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSI 107
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 130 bits (314), Expect = 1e-30
Identities = 57/85 (67%), Positives = 70/85 (82%)
Frame = +3
Query: 249 SYPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGM 428
S PEG ++CN+E +GDRG LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M
Sbjct: 107 SIPEGAVICNVELHVGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAM 166
Query: 429 VGIVAGGGRIDKPILKAGRAYHKYK 503
+G VAGGGR +KP LKAG AYHKYK
Sbjct: 167 IGQVAGGGRTEKPFLKAGNAYHKYK 191
Score = 89.8 bits (213), Expect = 2e-18
Identities = 39/59 (66%), Positives = 48/59 (81%)
Frame = +2
Query: 2 RKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 178
RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA V FR P+++ +KELF+A EG
Sbjct: 24 RKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRHPFRYMKQKELFVAAEG 82
Score = 79.4 bits (187), Expect = 2e-15
Identities = 31/38 (81%), Positives = 35/38 (92%)
Frame = +2
Query: 500 QGQRNCWPYVRGVAMNPVEHPHGGGNHQHIGKASTVKK 613
+ +RNCWP VRGVAMNPVEHPHGGGNHQHIG ASTV++
Sbjct: 191 KAKRNCWPVVRGVAMNPVEHPHGGGNHQHIGHASTVRR 228
Score = 44.0 bits (99), Expect = 1e-04
Identities = 16/26 (61%), Positives = 23/26 (88%)
Frame = +1
Query: 178 LYTGQFVYCGKXSTLEVGNVMPVGAI 255
+YTGQ++YCGK + L VGNV+P+G+I
Sbjct: 83 MYTGQYLYCGKKANLMVGNVLPLGSI 108
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 126 bits (304), Expect = 2e-29
Identities = 54/86 (62%), Positives = 69/86 (80%)
Frame = +3
Query: 249 SYPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGM 428
S PEG ++CN+E +GDRG ARASG++A VI HNPD +R+KLPSG+KK++PS R M
Sbjct: 106 SIPEGAVICNVEHHVGDRGVFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAM 165
Query: 429 VGIVAGGGRIDKPILKAGRAYHKYKV 506
+G VAGGGR +KP+LKAG AYHKY+V
Sbjct: 166 IGQVAGGGRTEKPMLKAGNAYHKYRV 191
Score = 93.1 bits (221), Expect = 2e-19
Identities = 41/59 (69%), Positives = 49/59 (83%)
Frame = +2
Query: 2 RKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEG 178
RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P++FK +KELF+A EG
Sbjct: 23 RKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHPFRFKKQKELFVAAEG 81
Score = 78.6 bits (185), Expect = 4e-15
Identities = 31/36 (86%), Positives = 34/36 (94%)
Frame = +2
Query: 506 QRNCWPYVRGVAMNPVEHPHGGGNHQHIGKASTVKK 613
+RNCWP VRGVAMNPVEHPHGGGNHQHIG ASTV++
Sbjct: 192 KRNCWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRR 227
Score = 44.0 bits (99), Expect = 1e-04
Identities = 17/26 (65%), Positives = 23/26 (88%)
Frame = +1
Query: 178 LYTGQFVYCGKXSTLEVGNVMPVGAI 255
+YTGQF+YCGK +TL VGNV+P+ +I
Sbjct: 82 MYTGQFLYCGKKATLVVGNVLPLRSI 107
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 44.4 bits (100), Expect = 8e-05
Identities = 24/76 (31%), Positives = 40/76 (52%)
Frame = +3
Query: 261 GTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 440
GTI+ N+E G ++ RA+G A ++ P + +KLPSG K + + R +G V
Sbjct: 71 GTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTV 129
Query: 441 AGGGRIDKPILKAGRA 488
+ K + KAG++
Sbjct: 130 SNPSHGTKKLYKAGQS 145
Score = 40.7 bits (91), Expect = 0.001
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = +2
Query: 521 PYVRGVAMNPVEHPHGGGNHQHIGKASTVKKRG 619
P VRGVAMNP +HPHGGG GK+ + RG
Sbjct: 152 PKVRGVAMNPCDHPHGGGE----GKSKSSGSRG 180
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 44.4 bits (100), Expect = 8e-05
Identities = 24/76 (31%), Positives = 40/76 (52%)
Frame = +3
Query: 261 GTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIV 440
GTI+ N+E G ++ RA+G A ++ P + +KLPSG K + + R +G V
Sbjct: 71 GTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKWINAKCRATIGTV 129
Query: 441 AGGGRIDKPILKAGRA 488
+ K + KAG++
Sbjct: 130 SNPSHGTKKLYKAGQS 145
Score = 40.7 bits (91), Expect = 0.001
Identities = 19/33 (57%), Positives = 22/33 (66%)
Frame = +2
Query: 521 PYVRGVAMNPVEHPHGGGNHQHIGKASTVKKRG 619
P VRGVAMNP +HPHGGG GK+ + RG
Sbjct: 152 PKVRGVAMNPCDHPHGGGE----GKSKSSGSRG 180
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/23 (78%), Positives = 19/23 (82%), Gaps = 1/23 (4%)
Frame = +2
Query: 506 QRNCWPYVRGVAM-NPVEHPHGG 571
+RN W VRGVAM NPVEHPHGG
Sbjct: 415 KRNMWAKVRGVAMMNPVEHPHGG 437
>At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 123
Score = 29.5 bits (63), Expect = 2.5
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -3
Query: 189 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 61
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 70 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 112
>At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 122
Score = 29.5 bits (63), Expect = 2.5
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -3
Query: 189 ACVEPSGAMKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 61
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 69 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 111
>At1g40129.1 68414.m04766 hypothetical protein
Length = 567
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = -3
Query: 360 HQDCVQSQWRSFQRHVPDDLYHPFSLQDCTQWYPQDSSNRHHIS 229
H+ +++ F + P D PF + CT W P +N IS
Sbjct: 325 HKQSPRAECNIFFKDKPPDATLPFKTRGCTLWRPIKKTNDREIS 368
>At2g40770.1 68415.m05030 SNF2 domain-containing protein / helicase
domain-containing protein / zinc finger (C3HC4 type RING
finger) family protein low similarity to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00628: PHD-finger, PF00097: Zinc finger, C3HC4
type (RING finger)
Length = 1648
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = -3
Query: 360 HQDCVQSQWRSFQRHVPDDLYHPFSLQDCTQWYPQDSSNRHHIS 229
H+ Q WRS + HV D+L P ++C W + H S
Sbjct: 667 HKFFKQVMWRSSKVHVADELQLP-PQEECVSWLKFSAIEEHFYS 709
>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 705
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = -3
Query: 237 HISNFKS*XLSTINKLACVEPSGAMKSSFLVL-NLYGS 127
H+S +K+ ++ + CV+P G K+ F+V+ NL+ S
Sbjct: 462 HVSKYKNSLVTKFFGVHCVKPVGGQKTRFIVMGNLFCS 499
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 5.7
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +2
Query: 443 WRWTY*QTYFESWKGIPQVQG 505
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At1g01120.1 68414.m00015 fatty acid elongase 3-ketoacyl-CoA
synthase 1 (KCS1) nearly identical to GB:AAC99312
GI:4091810 from [Arabidopsis thaliana]
Length = 528
Score = 28.3 bits (60), Expect = 5.7
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -1
Query: 317 TCQTTSITHFLFKIAHNGTLRIAPTGITFPT 225
+C T+I FL + GT+ + TG+TF T
Sbjct: 53 SCNVTTILFFLIILPLTGTVLVQLTGLTFDT 83
>At1g17970.1 68414.m02223 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 368
Score = 27.9 bits (59), Expect = 7.5
Identities = 17/46 (36%), Positives = 21/46 (45%)
Frame = -1
Query: 578 GYHHREDALQGSWQHHVHMASSYVDLVLVVCPSSFQNRFVNTSTSS 441
G H R + H+H A L VV P S NRF N++ SS
Sbjct: 7 GEHIRLRRARNQTIRHLHAADDDPPLSHVVLPISQPNRFCNSAMSS 52
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 27.5 bits (58), Expect = 9.9
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 499 YLWYALPAFKIGL 461
Y+WYALP + IGL
Sbjct: 301 YIWYALPGYAIGL 313
>At3g10470.1 68416.m01255 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 398
Score = 27.5 bits (58), Expect = 9.9
Identities = 20/68 (29%), Positives = 31/68 (45%)
Frame = -1
Query: 572 HHREDALQGSWQHHVHMASSYVDLVLVVCPSSFQNRFVNTSTSSNNTDHASVA*WQNLLG 393
H + A G +H H S+Y D V S V T+T +NN++H S+ + G
Sbjct: 204 HKKPKAAMGLHSNHDHKKSNYDDAV------SLHLNNVLTTTPNNNSNHRSLVVYGK--G 255
Query: 392 SRR*LYSC 369
S ++ C
Sbjct: 256 SNNKVHEC 263
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,039,494
Number of Sequences: 28952
Number of extensions: 415808
Number of successful extensions: 1114
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1077
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1111
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1643603136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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