Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30799
(832 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 54 1e-07
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 54 1e-07
At1g32190.1 68414.m03959 expressed protein 31 0.94
At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 3.8
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 28 6.6
At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E... 28 6.6
At1g77800.1 68414.m09059 PHD finger family protein contains Pfam... 28 6.6
At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.6
At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.6
At5g54140.1 68418.m06740 IAA-amino acid hydrolase, putative (ILL... 28 8.7
At4g14096.1 68417.m02176 F-box family protein contains F-box dom... 28 8.7
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 54.0 bits (124), Expect = 1e-07
Identities = 22/53 (41%), Positives = 33/53 (62%), Gaps = 3/53 (5%)
Frame = +3
Query: 93 DSMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEE 242
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E
Sbjct: 258 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDE 310
Score = 52.4 bits (120), Expect = 4e-07
Identities = 25/77 (32%), Positives = 41/77 (53%)
Frame = +2
Query: 257 MSCNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYES 436
++ N + G QR+D RK +F + + G + + G+W++RG E+ D D E
Sbjct: 316 VTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDMEL 374
Query: 437 YDWKKLDPSSEETKKLV 487
Y+W K+D S E K+ V
Sbjct: 375 YEWTKVDISDEAQKERV 391
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 54.0 bits (124), Expect = 1e-07
Identities = 22/53 (41%), Positives = 33/53 (62%), Gaps = 3/53 (5%)
Frame = +3
Query: 93 DSMPKGTFNMDDFKRVYSNEDE---AKSIPYFWEKFDPENYSIWYAEYKYPEE 242
D +P +DD+KR+YSN +I FW+ +DPE YS+W+ +YKY +E
Sbjct: 259 DLLPPSPMVLDDWKRLYSNTKSNFREVAIKGFWDMYDPEGYSLWFCDYKYNDE 311
Score = 52.4 bits (120), Expect = 4e-07
Identities = 25/77 (32%), Positives = 41/77 (53%)
Frame = +2
Query: 257 MSCNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISGVWVWRGKELVFPLSSDWQVDYES 436
++ N + G QR+D RK +F + + G + + G+W++RG E+ D D E
Sbjct: 317 VTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKGLWLFRGPEIP-KFIMDEVYDMEL 375
Query: 437 YDWKKLDPSSEETKKLV 487
Y+W K+D S E K+ V
Sbjct: 376 YEWTKVDISDEAQKERV 392
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 31.1 bits (67), Expect = 0.94
Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +3
Query: 303 CANRLSHPSACLVKTTIP-PSPECGCGAERSSCSRC 407
C + L PS K P PS CGCG C +C
Sbjct: 296 CCSGLCRPSCSCPKPRCPKPSCSCGCGCGDCGCFKC 331
>At5g15630.1 68418.m01829 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL4 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 431
Score = 29.1 bits (62), Expect = 3.8
Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 6/32 (18%)
Frame = +3
Query: 321 HPSACL-----VKTTIPPSPECGCGAE-RSSC 398
HPS C+ TI P P C CG E + SC
Sbjct: 214 HPSCCVSFSSFYNDTITPCPSCACGCENKKSC 245
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 28.3 bits (60), Expect = 6.6
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 139 STPTKTKLNLFLTSGRSLTPRTIPFGMPNT-NTLRNSL 249
+ PT L ++L G S+TP IPF T +RN++
Sbjct: 565 AAPTTITLTIYLDDGGSVTPVYIPFRSDQTVRDIRNAI 602
>At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E2;
identical to gi:2689242, SP:P42745
Length = 152
Score = 28.3 bits (60), Expect = 6.6
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -2
Query: 396 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 283
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g77800.1 68414.m09059 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 1423
Score = 28.3 bits (60), Expect = 6.6
Identities = 22/81 (27%), Positives = 32/81 (39%), Gaps = 3/81 (3%)
Frame = +3
Query: 300 KCANRLSHPSACLVKTTIPPSPECGCGAERSSCSRCRLIGRWTTS--PTTGRNWILRARR 473
KCA + P C + P G + +S + C L G T + TT W+
Sbjct: 1033 KCAKESTGPWYCELCAESSSEPSFNFGEKPNSSTECTLCGGTTGAFRKTTNGQWVHAFCA 1092
Query: 474 PRNLSRTTSRG-TKPTKTVES 533
+L T RG P + +ES
Sbjct: 1093 EWSLESTFRRGQINPVQGMES 1113
>At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 6.6
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -2
Query: 396 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 283
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 28.3 bits (60), Expect = 6.6
Identities = 12/38 (31%), Positives = 16/38 (42%)
Frame = -2
Query: 396 TSSFPRHTHTPEMVELLSSPNRQTDAKACLRILSNLWN 283
+ +P T V + PN D CL IL N W+
Sbjct: 60 SEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWS 97
>At5g54140.1 68418.m06740 IAA-amino acid hydrolase, putative (ILL3)
identical to IAA-amino acid hydrolase homolog ILL3
[Arabidopsis thaliana] gi|3420801|gb|AAC31939
Length = 428
Score = 27.9 bits (59), Expect = 8.7
Identities = 25/114 (21%), Positives = 46/114 (40%), Gaps = 2/114 (1%)
Frame = +3
Query: 438 TTGRNWILRARRPRNLSRTTSRGTKPTKTVESSTRARYFK*MPTNVPANVYTFHTNNYKK 617
T G NW+++ + + + K + Y P V + T K
Sbjct: 288 TNGINWLIKRLKEVVEGQAEVQRCKADIDMHEDDHPMY----PATVNDHKLHEFTEKVLK 343
Query: 618 LL--PKKETIPNC*IGWDGWAIFRETVPSNFIGFFIKNHPGN*IGFSNSPGFYL 773
LL P+K N + + +A +++ +P +IG I+N + +SP F+L
Sbjct: 344 LLLGPEKVKPANKVMAGEDFAFYQQKIPGYYIGIGIRNEEIGSVRSVHSPYFFL 397
>At4g14096.1 68417.m02176 F-box family protein contains F-box domain
Pfam:PF00646
Length = 468
Score = 27.9 bits (59), Expect = 8.7
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 118 TWMISSVSTPTKTKLNLFLTSGRSLTPRTIPFGMPN 225
TW +SVS PT +L F T R P+++ PN
Sbjct: 199 TWDFASVSVPTLKRLT-FSTQVRDEFPKSVSIDTPN 233
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,996,753
Number of Sequences: 28952
Number of extensions: 482248
Number of successful extensions: 1281
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1276
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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