Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30784
(881 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 40 0.002
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 38 0.007
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 38 0.012
At1g64030.1 68414.m07252 serpin family protein / serine protease... 36 0.027
At2g14540.1 68415.m01628 serpin family protein / serine protease... 36 0.047
At1g62170.1 68414.m07013 serpin family protein / serine protease... 33 0.33
At5g52280.1 68418.m06488 protein transport protein-related low s... 32 0.44
At2g35580.1 68415.m04357 serpin family protein / serine protease... 31 0.77
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 31 0.77
At2g29380.1 68415.m03569 protein phosphatase 2C, putative / PP2C... 31 1.0
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 31 1.0
At1g35930.1 68414.m04462 replication protein-related weak simila... 30 1.8
At3g17560.1 68416.m02242 F-box family protein contains F-box dom... 28 7.2
At1g33400.1 68414.m04135 tetratricopeptide repeat (TPR)-containi... 28 7.2
At3g10550.1 68416.m01266 expressed protein 28 9.5
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 39.9 bits (89), Expect = 0.002
Identities = 44/148 (29%), Positives = 69/148 (46%), Gaps = 3/148 (2%)
Frame = +1
Query: 271 SFQDSQMLLTSALYFKGQWTIPFNTSSTSKLPFYNSKGEKIGEVNMMYNRHTYPFSNIKI 450
+ + S ++L +A+YFKG W+ F+ + T K F+ G + +V M N Y ++
Sbjct: 96 TIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLDGTSV-KVPFMTN---YEDQYLRS 151
Query: 451 LNA-RVIELPYGVENR-LSMLTWCQTLVFHLASMFMKFKELSLDSFFEELRISKEEFSDD 624
+ +V+ LPY + R SM + LA + K S SFF+ I S
Sbjct: 152 YDGFKVLRLPYIEDQRQFSMYIYLPNDKEGLAPLLEKIG--SEPSFFDN-HIPLHCIS-- 206
Query: 625 EVDCF-IPRFKIESNLGMTSVLKNAMGI 705
V F IP+FK + VLK+ MG+
Sbjct: 207 -VGAFRIPKFKFSFEFNASEVLKD-MGL 232
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 38.3 bits (85), Expect = 0.007
Identities = 41/158 (25%), Positives = 68/158 (43%), Gaps = 4/158 (2%)
Frame = +1
Query: 271 SFQDSQMLLTSALYFKGQWTIPFNTSSTSKLPFYNSKGEKIGEVNMMYNRHTYPFSNIKI 450
+ ++S ++L +A+YFKG W+ F+ T F+ G + +V M N Y ++
Sbjct: 161 TIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHLLDGTMV-KVPFMTN---YKKQYLEY 216
Query: 451 LNA-RVIELPYGVENR-LSMLTWCQTLVFHLASMF--MKFKELSLDSFFEELRISKEEFS 618
+ +V+ LPY + R +M + L ++ + K LD+ RI E F
Sbjct: 217 YDGFKVLRLPYVEDQRQFAMYIYLPNDRDGLPTLLEEISSKPRFLDNHIPRQRILTEAFK 276
Query: 619 DDEVDCFIPRFKIESNLGMTSVLKNAMGIEDCLMRGKL 732
IP+FK + VLK MG+ G L
Sbjct: 277 -------IPKFKFSFEFKASDVLKE-MGLTLPFTHGSL 306
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 37.5 bits (83), Expect = 0.012
Identities = 40/155 (25%), Positives = 65/155 (41%), Gaps = 8/155 (5%)
Frame = +1
Query: 250 TKIVDTGSFQD-SQMLLTSALYFKGQWTIPFNTSSTSKLPFYNSKGEKIGEVNMMYNRHT 426
T+++ GS ++++ +ALYFKG W F+ S T + F+ G K+ M +
Sbjct: 151 TEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGNKVTAPFMTSKKKQ 210
Query: 427 Y--PFSNIKILNARVIELPY--GVENR-LSMLTWCQTLVFHLASMFMKFKELS--LDSFF 585
Y + K+L LPY G + R SM + L+ + K LD+
Sbjct: 211 YVSAYDGFKVLG-----LPYLQGQDKRQFSMYFYLPDANNGLSDLLDKIVSTPGFLDNHI 265
Query: 586 EELRISKEEFSDDEVDCFIPRFKIESNLGMTSVLK 690
++ EF IP+FK ++VLK
Sbjct: 266 PRRQVKVREFK-------IPKFKFSFGFDASNVLK 293
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 36.3 bits (80), Expect = 0.027
Identities = 38/150 (25%), Positives = 56/150 (37%), Gaps = 4/150 (2%)
Frame = +1
Query: 292 LLTSALYFKGQWTIPFNTSSTSKLPFY--NSKGEKIGEVNMMYNRHTYPFSNIKILNARV 465
+ +AL FKG W PF T FY N + ++ N++ + K+L
Sbjct: 167 IYANALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVPFMSSYENQYVRAYDGFKVLRLPY 226
Query: 466 IELPYGVENRLSMLTWCQTLVFHLASMFMKFKELS--LDSFFEELRISKEEFSDDEVDCF 639
+ SM + L + K LDS R E+F
Sbjct: 227 QRGSDDTNRKFSMYFYLPDKKDGLDDLLEKMASTPGFLDSHIPTYRDELEKFR------- 279
Query: 640 IPRFKIESNLGMTSVLKNAMGIEDCLMRGK 729
IP+FKIE +TSVL + +G+ M K
Sbjct: 280 IPKFKIEFGFSVTSVL-DRLGLRSMSMYHK 308
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 35.5 bits (78), Expect = 0.047
Identities = 33/135 (24%), Positives = 51/135 (37%), Gaps = 4/135 (2%)
Frame = +1
Query: 301 SALYFKGQWTIPFNTSSTSKLPFY--NSKGEKIGEVNMMYNRHTYPFSNIKILNARVIEL 474
+ALYFKG W F+ S T PF+ N K + + + + K+L +
Sbjct: 198 NALYFKGAWEKAFDKSMTRDKPFHLLNGKSVSVPFMRSYEKQFIEAYDGFKVLRLPYRQG 257
Query: 475 PYGVENRLSMLTWCQTLVFHLASMFMKFKELS--LDSFFEELRISKEEFSDDEVDCFIPR 648
SM + L ++ + LDS E R+ +F IP+
Sbjct: 258 RDDTNREFSMYLYLPDKKGELDNLLERITSNPGFLDSHIPEYRVDVGDFR-------IPK 310
Query: 649 FKIESNLGMTSVLKN 693
FKIE +SV +
Sbjct: 311 FKIEFGFEASSVFND 325
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 32.7 bits (71), Expect = 0.33
Identities = 31/135 (22%), Positives = 50/135 (37%), Gaps = 4/135 (2%)
Frame = +1
Query: 301 SALYFKGQWTIPFNTSSTSKLPFY--NSKGEKIGEVNMMYNRHTYPFSNIKILNARVIEL 474
SALYFKG W ++ S T PFY N + ++ ++ + K+L +
Sbjct: 234 SALYFKGTWEEKYSKSMTKCKPFYLLNGTSVSVPFMSSFEKQYIAAYDGFKVLRLPYRQG 293
Query: 475 PYGVENRLSMLTWCQTLVFHLASMFMKFKELS--LDSFFEELRISKEEFSDDEVDCFIPR 648
+M + L + + LDS E R+ +F IP+
Sbjct: 294 RDNTNRNFAMYIYLPDKKGELDDLLERMTSTPGFLDSHNPERRVKVGKFR-------IPK 346
Query: 649 FKIESNLGMTSVLKN 693
FKIE +S +
Sbjct: 347 FKIEFGFEASSAFSD 361
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 32.3 bits (70), Expect = 0.44
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +1
Query: 748 GLGTSYGLVVESGYXKAGN*RLPEAGNNQLAGVTSRRNSSQPDW 879
GLG S+ + ESG+ GN RLP+ N+ A R S+ DW
Sbjct: 189 GLGGSFDSIGESGWIDDGNARLPQRHNSVPATRNGHRRSN-TDW 231
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 31.5 bits (68), Expect = 0.77
Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = +1
Query: 292 LLTSALYFKGQWTIPFNTSSTSKLPFYNSKGEKIGEVNMMYN---RHTYPFSNIKILN 456
+ +AL+F G+W F+ S T F+ G K+ V M R+T+ + K++N
Sbjct: 167 IFANALFFNGRWDSQFDPSLTKDSDFHLLDGTKV-RVPFMTGASCRYTHVYEGFKVIN 223
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 31.5 bits (68), Expect = 0.77
Identities = 22/61 (36%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = +1
Query: 301 SALYFKGQWTIPFNTSSTSKLPFYNSKGEKIGEVNMMYNRHTYPFSNIKILNA-RVIELP 477
+ALYFKG W F+ SST F+ K EV++ + R +Y I + +V+ LP
Sbjct: 47 NALYFKGAWENKFDKSSTKDNEFHQGK-----EVHVPFMR-SYESQYIMACDGFKVLGLP 100
Query: 478 Y 480
Y
Sbjct: 101 Y 101
>At2g29380.1 68415.m03569 protein phosphatase 2C, putative / PP2C,
putative contains PF00481: Protein phosphatase 2C
domain; similar to protein phpsphatase 2C (PP2C)
(GI:7768151) [Fagus sylvatica].
Length = 362
Score = 31.1 bits (67), Expect = 1.0
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 595 RISKEEFSDDEVDCFIPRFKIESNLGMTSVLKNAMGI 705
R S+E+FSD VD PR+ + S G +++A+ I
Sbjct: 60 RSSREDFSDQNVDVSSPRYGVSSVCGRRREMEDAVAI 96
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 31.1 bits (67), Expect = 1.0
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1
Query: 301 SALYFKGQWTIPFNTSSTSKLPFYNSKGEKI 393
+ALYFKG W F S T PF+ G+++
Sbjct: 70 NALYFKGAWENKFGKSMTIHKPFHLVNGKQV 100
>At1g35930.1 68414.m04462 replication protein-related weak
similarity to Replication Protein A 70 kDa DNA-binding
subunit (RP-A) (RF-A) (Replication factor-A protein 1)
(Single-stranded DNA-binding protein) (DMRPA1)
(GI:2498844)(SP:Q24492) [Drosophila melanogaster]
Length = 261
Score = 30.3 bits (65), Expect = 1.8
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = -2
Query: 142 IVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEPF*DFLEFASSDVRML 2
+V V W+ +D + EF C R+ +P + +FAS +ML
Sbjct: 211 VVCAVRFWRIASVDGENVLKSEFGCSRLHIDPSFPYFDFASGVSKML 257
>At3g17560.1 68416.m02242 F-box family protein contains F-box domain
Pfam:PF00646
Length = 413
Score = 28.3 bits (60), Expect = 7.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 169 HISFVIGLCIVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEP 47
H+S + C +KV +W T ID + + +F + VD EP
Sbjct: 307 HLSLLYQSCETLKVEIWMTKEIDTTFVSWKKF--LTVDLEP 345
>At1g33400.1 68414.m04135 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515 TPR Domain
Length = 798
Score = 28.3 bits (60), Expect = 7.2
Identities = 16/51 (31%), Positives = 23/51 (45%), Gaps = 3/51 (5%)
Frame = -3
Query: 765 IGSPEPICG*NQLSSHQTILNTHGILQHRCHTQIRLNFESRN---ETIDFI 622
I + EPI Q+ Q + T + H C I L F SR +T +F+
Sbjct: 461 ISTKEPIQSLEQIRVGQALYKTGSLFNHSCKPNIHLYFLSRGLIMQTTEFV 511
>At3g10550.1 68416.m01266 expressed protein
Length = 634
Score = 27.9 bits (59), Expect = 9.5
Identities = 16/49 (32%), Positives = 28/49 (57%), Gaps = 5/49 (10%)
Frame = +1
Query: 388 KIGEVNMMYNR-HTYPFSNI---KILNARVIEL-PYGVENRLSMLTWCQ 519
+I ++N YN TYPF+ + I +A +++ + RL ++TWCQ
Sbjct: 17 RISDLNSNYNLCQTYPFAFMIPKSISDAELLQACSFRARCRLPVITWCQ 65
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,261,680
Number of Sequences: 28952
Number of extensions: 456808
Number of successful extensions: 1093
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1053
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1091
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2077687200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -