BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30769 (847 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 33 0.067 SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 27 4.4 SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 27 4.4 SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces... 27 4.4 SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces po... 26 7.7 SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 26 7.7 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 26 7.7 >SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 32.7 bits (71), Expect = 0.067 Identities = 26/80 (32%), Positives = 34/80 (42%), Gaps = 7/80 (8%) Frame = -3 Query: 299 CR*DSNCFSI--AHRLFIT--NFRL-QHHKTGRCPTENTQPIKDHNSLKKMKTYL--IIS 141 C+ D C+S+ HRL T F L C T + IK N L K L ++ Sbjct: 82 CQNDGKCYSVIAVHRLSFTLVMFHLFLAFILSLCNTRSRVAIKIQNGLWPFKIVLWFVLG 141 Query: 140 VRSIFIPNTYTLFWIEAFSV 81 + S FIP + FW SV Sbjct: 142 IFSFFIPTKFLSFWGNIISV 161 >SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 26.6 bits (56), Expect = 4.4 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = +1 Query: 253 INKRCAIEKQLLSHLQCFSVHKNPVSLENNY 345 +N A+EK+ +SH K+ V+ NNY Sbjct: 352 LNHISALEKETISHTDLHHTFKHEVNSSNNY 382 >SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr 1|||Manual Length = 1315 Score = 26.6 bits (56), Expect = 4.4 Identities = 17/51 (33%), Positives = 22/51 (43%) Frame = -3 Query: 215 CPTENTQPIKDHNSLKKMKTYLIISVRSIFIPNTYTLFWIEAFSVGPRQIR 63 C ++ K SLKK K + I R I +YTLF A P +R Sbjct: 813 CSADDVLIFKAVESLKKAKDTVDIEERQSLIELSYTLFKKAAHVFTPEDLR 863 >SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 279 Score = 26.6 bits (56), Expect = 4.4 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = -3 Query: 836 NTVEKPQTYFNAHCQSSKSTPKQNTV 759 N V +P+ F A CQ +STPK V Sbjct: 112 NAVGRPRPDFLARCQPFESTPKTGLV 137 >SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 25.8 bits (54), Expect = 7.7 Identities = 17/57 (29%), Positives = 27/57 (47%) Frame = -2 Query: 246 FPFTTS*NGTVSNRKHSAYQRSQFIKKNENLFDNLCTIHFHSEHIHTILDRGIFCWA 76 +PF T++ +HSA ++ + +EN TIH+ +H L R CWA Sbjct: 192 YPFNPQEVNTLNKHRHSASLQNLYKLLDENKVLMQGTIHW----LHGNLHRWSKCWA 244 >SPBC354.05c |sre2||membrane-tethered transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 793 Score = 25.8 bits (54), Expect = 7.7 Identities = 16/59 (27%), Positives = 26/59 (44%), Gaps = 2/59 (3%) Frame = -3 Query: 779 TPKQNTVVH-LLTLLKF-CFNHGAALMPDSLKYLWVISQYDKIALNTFLFRLMVLRIPI 609 +P ++++ LL LL F CF L P++ W S Y L ++ +PI Sbjct: 710 SPSLHSILRFLLLLLAFLCFAMHILLTPEATLRKWASSIYLSFRLECVFISFLIFSVPI 768 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 25.8 bits (54), Expect = 7.7 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +1 Query: 280 QLLSHLQCFSVHKNPVSLEN 339 QLL HL C +V++ VS EN Sbjct: 7 QLLDHLDCNAVYRGYVSQEN 26 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,546,581 Number of Sequences: 5004 Number of extensions: 73181 Number of successful extensions: 164 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 418457710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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