BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30769
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 33 0.067
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 27 4.4
SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 27 4.4
SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces... 27 4.4
SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces po... 26 7.7
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 26 7.7
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 26 7.7
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 32.7 bits (71), Expect = 0.067
Identities = 26/80 (32%), Positives = 34/80 (42%), Gaps = 7/80 (8%)
Frame = -3
Query: 299 CR*DSNCFSI--AHRLFIT--NFRL-QHHKTGRCPTENTQPIKDHNSLKKMKTYL--IIS 141
C+ D C+S+ HRL T F L C T + IK N L K L ++
Sbjct: 82 CQNDGKCYSVIAVHRLSFTLVMFHLFLAFILSLCNTRSRVAIKIQNGLWPFKIVLWFVLG 141
Query: 140 VRSIFIPNTYTLFWIEAFSV 81
+ S FIP + FW SV
Sbjct: 142 IFSFFIPTKFLSFWGNIISV 161
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 26.6 bits (56), Expect = 4.4
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +1
Query: 253 INKRCAIEKQLLSHLQCFSVHKNPVSLENNY 345
+N A+EK+ +SH K+ V+ NNY
Sbjct: 352 LNHISALEKETISHTDLHHTFKHEVNSSNNY 382
>SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1315
Score = 26.6 bits (56), Expect = 4.4
Identities = 17/51 (33%), Positives = 22/51 (43%)
Frame = -3
Query: 215 CPTENTQPIKDHNSLKKMKTYLIISVRSIFIPNTYTLFWIEAFSVGPRQIR 63
C ++ K SLKK K + I R I +YTLF A P +R
Sbjct: 813 CSADDVLIFKAVESLKKAKDTVDIEERQSLIELSYTLFKKAAHVFTPEDLR 863
>SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 279
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = -3
Query: 836 NTVEKPQTYFNAHCQSSKSTPKQNTV 759
N V +P+ F A CQ +STPK V
Sbjct: 112 NAVGRPRPDFLARCQPFESTPKTGLV 137
>SPCPB16A4.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 328
Score = 25.8 bits (54), Expect = 7.7
Identities = 17/57 (29%), Positives = 27/57 (47%)
Frame = -2
Query: 246 FPFTTS*NGTVSNRKHSAYQRSQFIKKNENLFDNLCTIHFHSEHIHTILDRGIFCWA 76
+PF T++ +HSA ++ + +EN TIH+ +H L R CWA
Sbjct: 192 YPFNPQEVNTLNKHRHSASLQNLYKLLDENKVLMQGTIHW----LHGNLHRWSKCWA 244
>SPBC354.05c |sre2||membrane-tethered transcription factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 793
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/59 (27%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -3
Query: 779 TPKQNTVVH-LLTLLKF-CFNHGAALMPDSLKYLWVISQYDKIALNTFLFRLMVLRIPI 609
+P ++++ LL LL F CF L P++ W S Y L ++ +PI
Sbjct: 710 SPSLHSILRFLLLLLAFLCFAMHILLTPEATLRKWASSIYLSFRLECVFISFLIFSVPI 768
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.8 bits (54), Expect = 7.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +1
Query: 280 QLLSHLQCFSVHKNPVSLEN 339
QLL HL C +V++ VS EN
Sbjct: 7 QLLDHLDCNAVYRGYVSQEN 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,546,581
Number of Sequences: 5004
Number of extensions: 73181
Number of successful extensions: 164
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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