BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30758 (776 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g01160.1 68416.m00020 expressed protein 29 3.4 At5g40710.1 68418.m04941 zinc finger (C2H2 type) family protein ... 28 6.0 At3g21850.1 68416.m02754 E3 ubiquitin ligase SCF complex subunit... 28 7.9 >At3g01160.1 68416.m00020 expressed protein Length = 380 Score = 29.1 bits (62), Expect = 3.4 Identities = 23/80 (28%), Positives = 37/80 (46%), Gaps = 3/80 (3%) Frame = +1 Query: 28 SHEKDTSTQVSEALIKIFVAQICKDLPEDT---AKLLINPNEDDISVTPLVVLFLQLEMV 198 S +KD ALI+ KDL E+ ++ N +D+S L Q E V Sbjct: 87 SKKKDKKKDKYRALIEAEDVDSDKDLEEENDQDMEVTFNTGLEDLSKEILKKKDNQSESV 146 Query: 199 AKSYLDNRRKTRRAKDKRPR 258 ++YL RR+ +RA+ + + Sbjct: 147 WETYLRQRREKKRARKNKQK 166 >At5g40710.1 68418.m04941 zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type Length = 272 Score = 28.3 bits (60), Expect = 6.0 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = -3 Query: 393 SRRFCLIMSTSCSPVIGGGTSSLLHSFCMR 304 +R C ++ SC PV G +++ LH F +R Sbjct: 172 NRHLCESLANSCFPVNKGSSANRLHDFFLR 201 >At3g21850.1 68416.m02754 E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 (At9), putative E3 ubiquitin ligase; similar to Skp1 homolog SKP1a GI:3068807 from [Arabidopsis thaliana] Length = 153 Score = 27.9 bits (59), Expect = 7.9 Identities = 14/60 (23%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +2 Query: 257 EENQSTTESGELNIEALIQNECNKLEVP-PPITGEQLVDMIRQNLREYVNFERPLHDDEI 433 E + ++ I + +N+C +P P +TG+ L +I + +V+ P DD++ Sbjct: 17 EVEEEAARQCQIIIAHMSENDCTDNGIPLPNVTGKILAMVIEYCNKHHVDAANPCSDDDL 76 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,189,635 Number of Sequences: 28952 Number of extensions: 272411 Number of successful extensions: 851 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 822 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 851 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1736283200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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