Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30739
         (687 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   118   8e-28
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    53   3e-08
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    50   4e-07
SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|...    31   0.21 

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  118 bits (284), Expect = 8e-28
 Identities = 66/135 (48%), Positives = 88/135 (65%), Gaps = 7/135 (5%)
 Frame = +1

Query: 1   NMMAACDPRHGRYLTVAAIFRGRMSMKG-GRRANA*HPEQELLILRGMDPQQRE------ 159
           NMM A DPRHGRYLTVAA+FRG++SMK    +  +   +     +  +     +      
Sbjct: 298 NMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVP 357

Query: 160 DRRVRHSSSWSQDGRHLHRKLHRHPGAVQAHLEQFTAMFRRKAFLHWYTGEGMDEMEFTE 339
            + ++ S+++  +   + +++ R  G      +QF+AMFRRKAFLHWYTGEGMDEMEFTE
Sbjct: 358 PKDLKMSATFIGNSTSI-QEIFRRLG------DQFSAMFRRKAFLHWYTGEGMDEMEFTE 410

Query: 340 AESNMNDLVSEYQQY 384
           AESNMNDLVSEYQQY
Sbjct: 411 AESNMNDLVSEYQQY 425



 Score = 95.9 bits (228), Expect = 5e-21
 Identities = 40/58 (68%), Positives = 52/58 (89%)
 Frame = +2

Query: 80  KEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRI 253
           KEVDEQ+ ++Q KNS+YFVEWIP+NV  AVC +PP+ LKM+ATFIGNST+IQE+F+R+
Sbjct: 324 KEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRL 381


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 38/135 (28%), Positives = 66/135 (48%), Gaps = 8/135 (5%)
 Frame = +1

Query: 1   NMMAACDPRHGRYLTVAAIFRGRMSMKGGRRANA*HPEQELLILRGMDPQQRE----DRR 168
           N M  CDPR GRY+    ++RG +  +  + A      +  +      P   +    DR 
Sbjct: 300 NQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRP 359

Query: 169 VRH--SSSWSQDGRHLHRKLHRHPGA-VQAHLE-QFTAMFRRKAFLHWYTGEGMDEMEFT 336
            +H   S  ++  R +    +    A   + L+ +F  M+ ++AF+HWY GEGM+E EF+
Sbjct: 360 PQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFS 419

Query: 337 EAESNMNDLVSEYQQ 381
           EA  ++  L  +Y++
Sbjct: 420 EAREDLAALERDYEE 434



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 18/66 (27%), Positives = 33/66 (50%), Gaps = 8/66 (12%)
 Frame = +2

Query: 80  KEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLK--------MAATFIGNSTAIQ 235
           ++V   +  I+ K +  FV+W P   K  +CD PP+ ++         A   + N+T+I 
Sbjct: 326 RDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIA 385

Query: 236 ELFKRI 253
           E + R+
Sbjct: 386 EAWSRL 391


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 18/41 (43%), Positives = 30/41 (73%)
 Frame = +1

Query: 259 QFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 381
           +F  M+ ++AF+HWY GEGM+E EF+EA  ++  L  +Y++
Sbjct: 398 KFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438



 Score = 33.1 bits (72), Expect = 0.039
 Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 8/66 (12%)
 Frame = +2

Query: 80  KEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPR--------GLKMAATFIGNSTAIQ 235
           ++V   + +I+++ +  FV+W P   K  +C  PP+         +  A   + N+T+I 
Sbjct: 330 RDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIA 389

Query: 236 ELFKRI 253
           E + R+
Sbjct: 390 EAWSRL 395



 Score = 32.3 bits (70), Expect = 0.068
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +1

Query: 1   NMMAACDPRHGRYLTVAAIFRG 66
           N M  CDPR GRY+    ++RG
Sbjct: 304 NQMVKCDPRTGRYMATCLLYRG 325


>SPBC365.12c |ish1||LEA domain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 684

 Score = 30.7 bits (66), Expect = 0.21
 Identities = 20/74 (27%), Positives = 33/74 (44%)
 Frame = +1

Query: 148 QQREDRRVRHSSSWSQDGRHLHRKLHRHPGAVQAHLEQFTAMFRRKAFLHWYTGEGMDEM 327
           Q+   RR R  + W+  G   +   H  P    + ++QF A  RR+ +L     + +   
Sbjct: 287 QEISKRRGRFGNWWANSGLKAYFDAHGIPAYQPSPIDQFYAHLRRQYYLKTNGYQALKSK 346

Query: 328 EFTEAESNMNDLVS 369
            + EA SN+ D  S
Sbjct: 347 AYEEA-SNVADSAS 359


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,446,967
Number of Sequences: 5004
Number of extensions: 44179
Number of successful extensions: 153
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)