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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30727
         (719 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g59090.1 68418.m07403 subtilase family protein contains simil...    33   0.25 
At5g59120.1 68418.m07409 subtilase family protein contains simil...    28   7.2  
At5g59130.1 68418.m07411 subtilase family protein contains simil...    27   9.5  

>At5g59090.1 68418.m07403 subtilase family protein contains
           similarity to prepro-cucumisin GI:807698 from [Cucumis
           melo]
          Length = 736

 Score = 32.7 bits (71), Expect = 0.25
 Identities = 20/76 (26%), Positives = 34/76 (44%)
 Frame = -1

Query: 650 LSNPNLPIKKLPKNVHGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFM 471
           +  PN   K      HG  ++  +  S+  +  +   ++FSV V       E+ SS + +
Sbjct: 655 VGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLI 714

Query: 470 YQHNLHNVLIPSIVYI 423
           +    HNV  P +VYI
Sbjct: 715 WSDGTHNVRSPIVVYI 730


>At5g59120.1 68418.m07409 subtilase family protein contains
           similarity to prepro-cucumisin GI:807698 from [Cucumis
           melo]; non-consensus AA acceptor site at exon 6
          Length = 732

 Score = 27.9 bits (59), Expect = 7.2
 Identities = 15/60 (25%), Positives = 29/60 (48%)
 Frame = -1

Query: 605 HGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFMYQHNLHNVLIPSIVY 426
           HG  ++  +  S+  +  +   ++F+V V       E+ SS + ++    HNV  P +VY
Sbjct: 669 HGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728


>At5g59130.1 68418.m07411 subtilase family protein contains
           similarity to prepro-cucumisin GI:807698 from [Cucumis
           melo]
          Length = 732

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 19/75 (25%), Positives = 32/75 (42%)
 Frame = -1

Query: 650 LSNPNLPIKKLPKNVHGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFM 471
           +  PN   K      HG  +N  +  S+     +   ++F+V V       E+ SS + +
Sbjct: 648 VGTPNSTYKSKVVLNHGSKLNVKVSPSVLSMKSMNEKQSFTVTVSASELHSELPSSANLI 707

Query: 470 YQHNLHNVLIPSIVY 426
           +    HNV  P +VY
Sbjct: 708 WSDGTHNVRSPIVVY 722


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,925,599
Number of Sequences: 28952
Number of extensions: 252444
Number of successful extensions: 476
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 467
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 476
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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