BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30727 (719 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g59090.1 68418.m07403 subtilase family protein contains simil... 33 0.25 At5g59120.1 68418.m07409 subtilase family protein contains simil... 28 7.2 At5g59130.1 68418.m07411 subtilase family protein contains simil... 27 9.5 >At5g59090.1 68418.m07403 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo] Length = 736 Score = 32.7 bits (71), Expect = 0.25 Identities = 20/76 (26%), Positives = 34/76 (44%) Frame = -1 Query: 650 LSNPNLPIKKLPKNVHGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFM 471 + PN K HG ++ + S+ + + ++FSV V E+ SS + + Sbjct: 655 VGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLI 714 Query: 470 YQHNLHNVLIPSIVYI 423 + HNV P +VYI Sbjct: 715 WSDGTHNVRSPIVVYI 730 >At5g59120.1 68418.m07409 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo]; non-consensus AA acceptor site at exon 6 Length = 732 Score = 27.9 bits (59), Expect = 7.2 Identities = 15/60 (25%), Positives = 29/60 (48%) Frame = -1 Query: 605 HGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFMYQHNLHNVLIPSIVY 426 HG ++ + S+ + + ++F+V V E+ SS + ++ HNV P +VY Sbjct: 669 HGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728 >At5g59130.1 68418.m07411 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo] Length = 732 Score = 27.5 bits (58), Expect = 9.5 Identities = 19/75 (25%), Positives = 32/75 (42%) Frame = -1 Query: 650 LSNPNLPIKKLPKNVHGPPINFSLKVSLRVYPIIISSRNFSVWVI*LI*QFEI*SSFDFM 471 + PN K HG +N + S+ + ++F+V V E+ SS + + Sbjct: 648 VGTPNSTYKSKVVLNHGSKLNVKVSPSVLSMKSMNEKQSFTVTVSASELHSELPSSANLI 707 Query: 470 YQHNLHNVLIPSIVY 426 + HNV P +VY Sbjct: 708 WSDGTHNVRSPIVVY 722 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,925,599 Number of Sequences: 28952 Number of extensions: 252444 Number of successful extensions: 476 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 467 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 476 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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