Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30725
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 170 2e-43
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 62 1e-10
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 57 3e-09
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 46 8e-06
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 38 0.001
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 32 0.076
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 31 0.13
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 30 0.41
SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr 3... 28 1.6
SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 27 2.2
SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|ch... 27 2.9
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 27 2.9
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 27 3.8
SPCC1620.03 |mug163||sequence orphan|Schizosaccharomyces pombe|c... 27 3.8
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 26 6.6
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.6
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 8.8
SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 25 8.8
SPBC2F12.10 |||mitochondrial ribosomal protein subunit L35|Schiz... 25 8.8
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 170 bits (413), Expect = 2e-43
Identities = 101/209 (48%), Positives = 129/209 (61%), Gaps = 7/209 (3%)
Frame = +1
Query: 1 LAVDIALDAVNTVTVNDNGRIEVDIKNYAKVEKIPGGTVEESRVLSGVMFNKDVTHPKMR 180
L +AL AV TV NGR+E+DIK YA+VEK+PGG +E S VL GVM NKDVTHPKMR
Sbjct: 169 LMCHLALRAVRTVASTSNGRMEIDIKRYARVEKVPGGEIESSCVLDGVMLNKDVTHPKMR 228
Query: 181 RYIENPRIILLDCPLEYKKGESQTKLRL-----WVNRTSLNCYNWKRNM-YSVNVK-KLL 339
R IENPRI+LLDCPLEY+KGESQT + + W + KR Y + VK L+
Sbjct: 229 RRIENPRIVLLDCPLEYRKGESQTNIEISKDTDWNRILEIEEEQVKRMCDYIIAVKPDLV 288
Query: 340 HLNQILSLPKREFQILHNITXXXXXXXXSADSVKRDNNRLARACGATIVNRN*GTQESDV 519
+ +S + + + NIT + K DNNR+ARACGA IVNR +E DV
Sbjct: 289 ITEKGVSDLAQHYLLKANITALRR-------TRKSDNNRIARACGANIVNRLEDLREKDV 341
Query: 520 GTGAGLFEIQEDSEMKYFHFWSLQCQNPQ 606
GTG GLF I + + +Y+ F + C+NP+
Sbjct: 342 GTGCGLFYIDKLGD-EYYTFLT-GCKNPK 368
Score = 94.7 bits (225), Expect = 1e-20
Identities = 43/75 (57%), Positives = 57/75 (76%)
Frame = +3
Query: 219 PT*IQKGRESDQIEIMGEQDFTKLLQLEEEHVQRQCEEIIALKPDIVFTEKGVSDLAQHY 398
P +KG IEI + D+ ++L++EEE V+R C+ IIA+KPD+V TEKGVSDLAQHY
Sbjct: 242 PLEYRKGESQTNIEISKDTDWNRILEIEEEQVKRMCDYIIAVKPDLVITEKGVSDLAQHY 301
Query: 399 LVKAGITAIRRLRKT 443
L+KA ITA+RR RK+
Sbjct: 302 LLKANITALRRTRKS 316
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 61.7 bits (143), Expect = 1e-10
Identities = 33/83 (39%), Positives = 57/83 (68%)
Frame = +1
Query: 10 DIALDAVNTVTVNDNGRIEVDIKNYAKVEKIPGGTVEESRVLSGVMFNKDVTHPKMRRYI 189
+IA+DAV ++V D R +VD + KV+ GG+V++++++ GV+ +KD++HP+M I
Sbjct: 189 NIAVDAV--LSVADLQRKDVDFE-LIKVDGKVGGSVDDTKLVKGVVVDKDMSHPQMPHRI 245
Query: 190 ENPRIILLDCPLEYKKGESQTKL 258
EN +I +L CP E K +++ KL
Sbjct: 246 ENAKIAILTCPFEPPKPKTKHKL 268
Score = 29.9 bits (64), Expect = 0.41
Identities = 15/65 (23%), Positives = 30/65 (46%)
Frame = +3
Query: 234 KGRESDQIEIMGEQDFTKLLQLEEEHVQRQCEEIIALKPDIVFTEKGVSDLAQHYLVKAG 413
K + +++I +F L E+E Q + + ++V + G D A H L++
Sbjct: 261 KPKTKHKLDITSVSEFEALQAYEKEKFQEMIKHVKDAGANLVICQWGFDDEANHLLLQNN 320
Query: 414 ITAIR 428
+ A+R
Sbjct: 321 LPAVR 325
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 56.8 bits (131), Expect = 3e-09
Identities = 22/62 (35%), Positives = 42/62 (67%)
Frame = +1
Query: 64 EVDIKNYAKVEKIPGGTVEESRVLSGVMFNKDVTHPKMRRYIENPRIILLDCPLEYKKGE 243
++D+++Y K++KIPGG++++ +++GV+F+K + M R + PRI LL L+Y E
Sbjct: 518 DIDVRSYVKIKKIPGGSIQDCFLVNGVLFSKKASSKSMDRSLRRPRIALLTFSLDYACDE 577
Query: 244 SQ 249
+
Sbjct: 578 QR 579
Score = 31.9 bits (69), Expect = 0.10
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = +3
Query: 288 LLQLEEEHVQRQCEEIIALKPDIVFTEKGVSDLAQHYLVKAGITAIRRLRKT 443
++ +EE++ I LKP++VF + + +A Y + G+ A L+++
Sbjct: 586 IISQQEEYIINLVNRICMLKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKES 637
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 45.6 bits (103), Expect = 8e-06
Identities = 20/66 (30%), Positives = 40/66 (60%), Gaps = 3/66 (4%)
Frame = +1
Query: 76 KNYAKVEKIPGGTVEESRVLSGVMFNKDVTH---PKMRRYIENPRIILLDCPLEYKKGES 246
+N ++K+PGG +E+S ++ GV F K ++ + ++ +NP+I+ LD LE K +
Sbjct: 197 ENMIGIKKVPGGAMEDSLLVKGVAFKKTFSYAGFEQQPKFFKNPKILCLDVELELKAEKD 256
Query: 247 QTKLRL 264
++R+
Sbjct: 257 NAEVRV 262
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/75 (24%), Positives = 43/75 (57%)
Frame = +1
Query: 19 LDAVNTVTVNDNGRIEVDIKNYAKVEKIPGGTVEESRVLSGVMFNKDVTHPKMRRYIENP 198
+DA+ T+ D +D+ + ++ K+ + +++++ G++ + HP M + ++N
Sbjct: 171 VDAILTIRRPDE---PIDL-HMVEIMKMQNRSASDTQLIRGLLLDHGARHPDMPKQVKNA 226
Query: 199 RIILLDCPLEYKKGE 243
I++L+ LEY+K E
Sbjct: 227 YILILNVSLEYEKSE 241
Score = 29.1 bits (62), Expect = 0.71
Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 10/66 (15%)
Frame = +3
Query: 285 KLLQLEEEHVQRQCEEIIALKPDI----------VFTEKGVSDLAQHYLVKAGITAIRRL 434
+L++ E + V + +I+ LK ++ + +KG+ L+ L K GI A+RR
Sbjct: 256 RLVESERKFVDNKLRKIVELKKEVCERDPTANFVIINQKGIDPLSLDVLAKNGIMALRRA 315
Query: 435 RKTGQQ 452
++ +
Sbjct: 316 KRRNME 321
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 32.3 bits (70), Expect = 0.076
Identities = 14/52 (26%), Positives = 29/52 (55%)
Frame = +3
Query: 285 KLLQLEEEHVQRQCEEIIALKPDIVFTEKGVSDLAQHYLVKAGITAIRRLRK 440
K+ + E + ++I+ +++ T KG+ DL +++AG A+RR +K
Sbjct: 267 KIREREVMITLERVKKILNAGANVILTTKGIDDLCLKSIIEAGAMAVRRCKK 318
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 31.5 bits (68), Expect = 0.13
Identities = 16/69 (23%), Positives = 36/69 (52%), Gaps = 5/69 (7%)
Frame = +3
Query: 249 DQIEIMGEQDFTKLLQLEEEHVQRQCEEIIALKPDIVFTEKG-----VSDLAQHYLVKAG 413
+Q+ + + K+L+ E +++ C++I +++ +K V+DLA H+L K
Sbjct: 251 NQVVVNDYRQMDKILKEERQYLLNMCKKIKKAGANVILIQKSILRDAVNDLALHFLAKLK 310
Query: 414 ITAIRRLRK 440
I I+ + +
Sbjct: 311 IMVIKDIER 319
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 29.9 bits (64), Expect = 0.41
Identities = 20/50 (40%), Positives = 25/50 (50%)
Frame = +3
Query: 222 T*IQKGRESDQIEIMGEQDFTKLLQLEEEHVQRQCEEIIALKPDIVFTEK 371
T + KGR + +I E T+L+ LE CEE IAL VF EK
Sbjct: 1038 TVLLKGRVACEINSTSELVLTELI-LENSLADFSCEETIALLSAFVFDEK 1086
>SPCC736.12c |||conserved protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 437
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +3
Query: 306 EHVQRQCEEIIALKPDIVFTEKGVSDLAQHYLVKA-GITAIRRLRKT 443
EH + E I + P +V E G+S ++++++ TAI +KT
Sbjct: 323 EHRNEKGERISMINPRVVLDENGISHRSRYFIMLCDNETAIAHAKKT 369
>SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 521
Score = 27.5 bits (58), Expect = 2.2
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = -1
Query: 567 FHLRIFLNLKQSSSSTDIGFLSSSVTV 487
F L F+N K SSS+T G+ SS+VTV
Sbjct: 392 FGLLAFMNTK-SSSATIFGYFSSAVTV 417
>SPBC354.09c |||Tre1 family protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 794
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -3
Query: 631 RSNNWCQALGGSGTAVTKSGNISSPNLLESQTVQLQYRHRILEFLS 494
+ +NWCQ L + V ISSP L + ++ + +I+ ++
Sbjct: 386 KDSNWCQDLAPVLSEVWTGSKISSPGLEVNVLQDIEDKQKIINIMA 431
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/31 (45%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = +3
Query: 87 KSGKD--PRWHS*RVSCTQRCYVQQGCNPSE 173
+SGKD P WHS S T + V+Q +P E
Sbjct: 663 ESGKDKSPHWHSHGGSITGKSIVEQASSPLE 693
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -1
Query: 204 NARIFNIPPHLRMGYILVEHNTAEYTR 124
N I N+P L G ++E++TAE R
Sbjct: 94 NCEILNLPNGLSKGCAIIEYSTAEEAR 120
>SPCC1620.03 |mug163||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 186
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +3
Query: 303 EEHVQRQCEEIIALKPD 353
E + RQCE+I ALKPD
Sbjct: 167 ERNRSRQCEKISALKPD 183
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 67 VDIKNYAKVEKIPGGTVEESRVLSGVMFNKDVTHPKMRRYIENPRIILLD 216
VD+ + K+E IPG V R + V + + + P + + P II L+
Sbjct: 835 VDLSGFEKLEDIPGKVVVFERKICAV-YPVEGSSPPLGEGLNVPAIITLE 883
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 25.8 bits (54), Expect = 6.6
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = -2
Query: 143 TPLSTRDSSTVPPGIFSTFA*FLMSTSILPLSFTVTVLTASRAMST 6
T S+ SST P ST + S+S P+S ++T +S A S+
Sbjct: 137 TSASSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSS 182
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 25.4 bits (53), Expect = 8.8
Identities = 15/64 (23%), Positives = 34/64 (53%)
Frame = +3
Query: 129 CTQRCYVQQGCNPSEDEEVY*KSAHYPVGLPT*IQKGRESDQIEIMGEQDFTKLLQLEEE 308
C + ++Q +PS+DE + + P+G T + GR + ++++ +Q + + EE
Sbjct: 2237 CKKVAHIQY--DPSKDEVGAFAADYTPLGPNTFLAWGRVDNTVQLISDQLDKQPVMFEEL 2294
Query: 309 HVQR 320
H ++
Sbjct: 2295 HSEK 2298
>SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 675
Score = 25.4 bits (53), Expect = 8.8
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -1
Query: 144 NTAEYTRLFNCATWDLFHFCI 82
N A Y+ L N TW+LF CI
Sbjct: 516 NAANYSAL-NRVTWELFDICI 535
>SPBC2F12.10 |||mitochondrial ribosomal protein subunit
L35|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 25.4 bits (53), Expect = 8.8
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +1
Query: 619 NYYSVGGFLKDVPEIEDLKRESFQGWLFNNV 711
N+YSV DVP E + E+ WL N+
Sbjct: 233 NHYSVITLDLDVPNYETNRFETHCNWLLTNI 263
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,287,095
Number of Sequences: 5004
Number of extensions: 71950
Number of successful extensions: 237
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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