Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30705
(538 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 115 5e-27
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 76 4e-15
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 75 9e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 4e-13
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.8
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.3
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 26 3.1
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 3.1
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 115 bits (276), Expect = 5e-27
Identities = 56/94 (59%), Positives = 67/94 (71%)
Frame = +3
Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKES 434
LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124
Query: 435 ESWRLPTGLPTYTFPSVAATGFRMGSSLSQRFGE 536
E+ G T TG MG+ L + E
Sbjct: 125 EACDALQGF-QLTHSLGGGTGSGMGTLLLSKIRE 157
Score = 113 bits (271), Expect = 2e-26
Identities = 46/64 (71%), Positives = 55/64 (85%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 243
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 244 PRAI 255
PRA+
Sbjct: 61 PRAV 64
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 75.8 bits (178), Expect = 4e-15
Identities = 40/97 (41%), Positives = 62/97 (63%), Gaps = 3/97 (3%)
Frame = +3
Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422
PR + VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 423 RKESESWRLPTGLPTY-TFPSV-AATGFRMGSSLSQR 527
R+ +++ +GL + F S TG +G+ L +R
Sbjct: 127 RRMADNC---SGLQGFLVFHSFGGGTGSGLGALLLER 160
Score = 50.4 bits (115), Expect = 2e-07
Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 225
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 226 SGGKYVPRAI 255
GK+VPR+I
Sbjct: 61 GQGKFVPRSI 70
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 74.5 bits (175), Expect = 9e-15
Identities = 39/96 (40%), Positives = 59/96 (61%), Gaps = 2/96 (2%)
Frame = +3
Query: 246 PRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 422
PR + VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 423 RKESESWR-LPTGLPTYTFPSVAATGFRMGSSLSQR 527
R+ +++ L L ++F +GF G+ L +R
Sbjct: 123 RRIADNCSGLQGFLVFHSFGGGTGSGF--GALLLER 156
Score = 56.4 bits (130), Expect = 3e-09
Identities = 27/66 (40%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
Frame = +1
Query: 64 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 237
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 238 YVPRAI 255
YVPR+I
Sbjct: 61 YVPRSI 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 68.9 bits (161), Expect = 4e-13
Identities = 29/63 (46%), Positives = 44/63 (69%)
Frame = +1
Query: 67 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 246
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 247 RAI 255
RAI
Sbjct: 63 RAI 65
Score = 55.6 bits (128), Expect = 4e-09
Identities = 27/96 (28%), Positives = 53/96 (55%), Gaps = 2/96 (2%)
Frame = +3
Query: 255 LVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRK 428
L+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124
Query: 429 ESESWRLPTGLPTYTFPSVAATGFRMGSSLSQRFGE 536
E++ G + TG +GS L +R +
Sbjct: 125 EADGSDSLEGF-SLLHSIAGGTGSGLGSFLLERLND 159
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.8
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 103 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 204
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 2.3
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 366 AKGHYTEGAELVDSVLDVVRKESES 440
A+GH G ELV + D +RK+SE+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSEN 207
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 297 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 398
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 26.2 bits (55), Expect = 3.1
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = +3
Query: 231 RQVRAPRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 359
R+V AP+H GT RS +FR ++ FGQ GN
Sbjct: 486 REVFAPKH-ARRRLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 3.1
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 47 KITYTK*GKSFIYRPANVV 103
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 5.4
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 355 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 260
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,250,591
Number of Sequences: 5004
Number of extensions: 43799
Number of successful extensions: 126
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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