BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30689
(785 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g32190.1 68417.m04581 centromeric protein-related low similar... 31 1.1
At2g44930.1 68415.m05593 expressed protein contains Pfam profile... 30 1.5
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 29 2.6
At1g73840.1 68414.m08549 hydroxyproline-rich glycoprotein family... 29 3.5
At4g19865.1 68417.m02911 kelch repeat-containing F-box family pr... 29 4.6
At1g63810.1 68414.m07221 nucleolar RNA-associated family protein... 29 4.6
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 28 6.1
At3g43460.1 68416.m04607 hypothetical protein 28 6.1
>At4g32190.1 68417.m04581 centromeric protein-related low similarity
to SP|Q02224 Centromeric protein E (CENP-E protein)
{Homo sapiens}
Length = 783
Score = 30.7 bits (66), Expect = 1.1
Identities = 25/68 (36%), Positives = 42/68 (61%), Gaps = 3/68 (4%)
Frame = +2
Query: 98 ERKRDKPILELSKHSKKFDISSLQDN-YLKYDP-SVPQNLQIVEMDSNILDLK-ETFVMK 268
E +++KP+LEL+ H DIS +QD Y K + V QNL + E +S++++ K E +K
Sbjct: 421 ELQKEKPLLELAMH----DISVIQDELYKKANAFQVSQNL-LQEKESSLVEAKLEIQHLK 475
Query: 269 ARRSRLGL 292
+ ++ L L
Sbjct: 476 SEQASLEL 483
>At2g44930.1 68415.m05593 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 515
Score = 30.3 bits (65), Expect = 1.5
Identities = 22/84 (26%), Positives = 42/84 (50%), Gaps = 3/84 (3%)
Frame = +2
Query: 29 VPHEARQLSIHIATPIPLHIALRERKRDKPILELSKHSKKF-DISSLQDNYLKYDPSVPQ 205
VP+ R+++ TP L I + + LELSK ++ D +++ + KY ++
Sbjct: 89 VPNRLRRVNPEAYTPQMLLIGPLQHSKKANALELSKTDLRYLDYMNMELHKKKYLNAIAN 148
Query: 206 NL--QIVEMDSNILDLKETFVMKA 271
QI+E+ I++ E FV ++
Sbjct: 149 KYGDQIIEVFKEIIETSEKFVRES 172
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/60 (26%), Positives = 25/60 (41%)
Frame = +2
Query: 56 IHIATPIPLHIALRERKRDKPILELSKHSKKFDISSLQDNYLKYDPSVPQNLQIVEMDSN 235
I I T P A+R R + I S + F +D YL+ S+P++ + N
Sbjct: 121 ILIFTVDPFSFAVRRRSNGETIFNTSSSDESFGEMVFKDQYLEISTSLPKDASLYGFGEN 180
>At1g73840.1 68414.m08549 hydroxyproline-rich glycoprotein family
protein similar to proline-rich protein precursor
GB:AAC34889 [Glycine max]
Length = 388
Score = 29.1 bits (62), Expect = 3.5
Identities = 14/48 (29%), Positives = 19/48 (39%), Gaps = 1/48 (2%)
Frame = +1
Query: 556 FHSKRKQCYRGREV-YSKHSVSTSHCASVVQKPANNPPAPWQPLFPLG 696
+H + Q G + H T H + + N PPA P FP G
Sbjct: 226 YHHQYGQAQTGPNTGFQHHGAPTQHLSQPMYHSGNRPPASGGPQFPQG 273
>At4g19865.1 68417.m02911 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 393
Score = 28.7 bits (61), Expect = 4.6
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 89 QCGGESVWLCVWTVDVLRAAPSSCE 15
+C G VW V +VDVL P SC+
Sbjct: 360 KCDGGGVWGIVESVDVLYTVPISCQ 384
>At1g63810.1 68414.m07221 nucleolar RNA-associated family protein /
Nrap family protein contains Pfam profile PF03813: Nrap
protein; similar to nucleolar RNA-associated protein
alpha (GI:18539461) [Mus musculus]
Length = 1053
Score = 28.7 bits (61), Expect = 4.6
Identities = 31/96 (32%), Positives = 44/96 (45%), Gaps = 5/96 (5%)
Frame = -2
Query: 784 PGNNRPAVRKRPPEAKDFPR-VCFPSKLNQGQGGITVAKGLADCWRAFERRKHNDWLTRC 608
P NN AV + +PR + FPS+LNQG+ VA+G A R F L R
Sbjct: 905 PLNNYDAVILLHRDKLPYPRRLLFPSELNQGK---HVARGKAS--RLFNPFMSPGDLKRS 959
Query: 607 ---VLNKLL-SLDNIACVLNGTQNNCRS*TPLLPHV 512
+ NKL+ + C+L+G Q + P H+
Sbjct: 960 HEELKNKLMVDFEPTKCLLSGLQEEFGTLKPWYDHI 995
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 28.3 bits (60), Expect = 6.1
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +3
Query: 384 SYIRRCGQCGRVSQLQHSNW 443
S I+RC QC + LQH NW
Sbjct: 849 SRIKRCLQCQPATTLQHVNW 868
>At3g43460.1 68416.m04607 hypothetical protein
Length = 702
Score = 28.3 bits (60), Expect = 6.1
Identities = 22/85 (25%), Positives = 34/85 (40%), Gaps = 1/85 (1%)
Frame = +1
Query: 319 DIYITSESRLQTDLMIMAIPVNPTLEDAVSVDVSVN-YSTPTGRTRPLEYSCEFVWASDD 495
D+ TS+ D + +P D +N YS+P LE EF+ S D
Sbjct: 18 DLSTTSKDSESEDGVDHGLPERVLARGFFPKDARLNIYSSP----ELLEEIAEFLGGSSD 73
Query: 496 WEECIRHAVATVFKNDNCSVFHSKR 570
+E ++ +FK C HS +
Sbjct: 74 FERLLKSQFGKLFKYPVCQTAHSAK 98
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,915,701
Number of Sequences: 28952
Number of extensions: 425141
Number of successful extensions: 1289
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1288
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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