BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30689 (785 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g32190.1 68417.m04581 centromeric protein-related low similar... 31 1.1 At2g44930.1 68415.m05593 expressed protein contains Pfam profile... 30 1.5 At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 29 2.6 At1g73840.1 68414.m08549 hydroxyproline-rich glycoprotein family... 29 3.5 At4g19865.1 68417.m02911 kelch repeat-containing F-box family pr... 29 4.6 At1g63810.1 68414.m07221 nucleolar RNA-associated family protein... 29 4.6 At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 28 6.1 At3g43460.1 68416.m04607 hypothetical protein 28 6.1 >At4g32190.1 68417.m04581 centromeric protein-related low similarity to SP|Q02224 Centromeric protein E (CENP-E protein) {Homo sapiens} Length = 783 Score = 30.7 bits (66), Expect = 1.1 Identities = 25/68 (36%), Positives = 42/68 (61%), Gaps = 3/68 (4%) Frame = +2 Query: 98 ERKRDKPILELSKHSKKFDISSLQDN-YLKYDP-SVPQNLQIVEMDSNILDLK-ETFVMK 268 E +++KP+LEL+ H DIS +QD Y K + V QNL + E +S++++ K E +K Sbjct: 421 ELQKEKPLLELAMH----DISVIQDELYKKANAFQVSQNL-LQEKESSLVEAKLEIQHLK 475 Query: 269 ARRSRLGL 292 + ++ L L Sbjct: 476 SEQASLEL 483 >At2g44930.1 68415.m05593 expressed protein contains Pfam profile PF03140: Plant protein of unknown function Length = 515 Score = 30.3 bits (65), Expect = 1.5 Identities = 22/84 (26%), Positives = 42/84 (50%), Gaps = 3/84 (3%) Frame = +2 Query: 29 VPHEARQLSIHIATPIPLHIALRERKRDKPILELSKHSKKF-DISSLQDNYLKYDPSVPQ 205 VP+ R+++ TP L I + + LELSK ++ D +++ + KY ++ Sbjct: 89 VPNRLRRVNPEAYTPQMLLIGPLQHSKKANALELSKTDLRYLDYMNMELHKKKYLNAIAN 148 Query: 206 NL--QIVEMDSNILDLKETFVMKA 271 QI+E+ I++ E FV ++ Sbjct: 149 KYGDQIIEVFKEIIETSEKFVRES 172 >At3g45940.1 68416.m04971 alpha-xylosidase, putative strong similarity to alpha-xylosidase precursor GI:4163997 from [Arabidopsis thaliana] Length = 868 Score = 29.5 bits (63), Expect = 2.6 Identities = 16/60 (26%), Positives = 25/60 (41%) Frame = +2 Query: 56 IHIATPIPLHIALRERKRDKPILELSKHSKKFDISSLQDNYLKYDPSVPQNLQIVEMDSN 235 I I T P A+R R + I S + F +D YL+ S+P++ + N Sbjct: 121 ILIFTVDPFSFAVRRRSNGETIFNTSSSDESFGEMVFKDQYLEISTSLPKDASLYGFGEN 180 >At1g73840.1 68414.m08549 hydroxyproline-rich glycoprotein family protein similar to proline-rich protein precursor GB:AAC34889 [Glycine max] Length = 388 Score = 29.1 bits (62), Expect = 3.5 Identities = 14/48 (29%), Positives = 19/48 (39%), Gaps = 1/48 (2%) Frame = +1 Query: 556 FHSKRKQCYRGREV-YSKHSVSTSHCASVVQKPANNPPAPWQPLFPLG 696 +H + Q G + H T H + + N PPA P FP G Sbjct: 226 YHHQYGQAQTGPNTGFQHHGAPTQHLSQPMYHSGNRPPASGGPQFPQG 273 >At4g19865.1 68417.m02911 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 393 Score = 28.7 bits (61), Expect = 4.6 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = -1 Query: 89 QCGGESVWLCVWTVDVLRAAPSSCE 15 +C G VW V +VDVL P SC+ Sbjct: 360 KCDGGGVWGIVESVDVLYTVPISCQ 384 >At1g63810.1 68414.m07221 nucleolar RNA-associated family protein / Nrap family protein contains Pfam profile PF03813: Nrap protein; similar to nucleolar RNA-associated protein alpha (GI:18539461) [Mus musculus] Length = 1053 Score = 28.7 bits (61), Expect = 4.6 Identities = 31/96 (32%), Positives = 44/96 (45%), Gaps = 5/96 (5%) Frame = -2 Query: 784 PGNNRPAVRKRPPEAKDFPR-VCFPSKLNQGQGGITVAKGLADCWRAFERRKHNDWLTRC 608 P NN AV + +PR + FPS+LNQG+ VA+G A R F L R Sbjct: 905 PLNNYDAVILLHRDKLPYPRRLLFPSELNQGK---HVARGKAS--RLFNPFMSPGDLKRS 959 Query: 607 ---VLNKLL-SLDNIACVLNGTQNNCRS*TPLLPHV 512 + NKL+ + C+L+G Q + P H+ Sbjct: 960 HEELKNKLMVDFEPTKCLLSGLQEEFGTLKPWYDHI 995 >At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 889 Score = 28.3 bits (60), Expect = 6.1 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +3 Query: 384 SYIRRCGQCGRVSQLQHSNW 443 S I+RC QC + LQH NW Sbjct: 849 SRIKRCLQCQPATTLQHVNW 868 >At3g43460.1 68416.m04607 hypothetical protein Length = 702 Score = 28.3 bits (60), Expect = 6.1 Identities = 22/85 (25%), Positives = 34/85 (40%), Gaps = 1/85 (1%) Frame = +1 Query: 319 DIYITSESRLQTDLMIMAIPVNPTLEDAVSVDVSVN-YSTPTGRTRPLEYSCEFVWASDD 495 D+ TS+ D + +P D +N YS+P LE EF+ S D Sbjct: 18 DLSTTSKDSESEDGVDHGLPERVLARGFFPKDARLNIYSSP----ELLEEIAEFLGGSSD 73 Query: 496 WEECIRHAVATVFKNDNCSVFHSKR 570 +E ++ +FK C HS + Sbjct: 74 FERLLKSQFGKLFKYPVCQTAHSAK 98 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,915,701 Number of Sequences: 28952 Number of extensions: 425141 Number of successful extensions: 1289 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1239 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1288 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1765546400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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