Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30679
(834 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2; Endopter... 180 3e-44
UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep: CG3... 102 1e-20
UniRef50_P43307 Cluster: Translocon-associated protein subunit a... 85 2e-15
UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella ve... 81 3e-14
UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2; ... 75 3e-12
UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;... 71 3e-11
UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole... 59 1e-07
UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1; ... 49 2e-04
UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2; Schis... 48 3e-04
UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole... 38 0.31
UniRef50_A0RNE9 Cluster: Peptidase, M48 family; n=2; Campylobact... 34 3.8
UniRef50_A3JW84 Cluster: Sensor protein; n=2; Rhodobacterales ba... 34 5.1
UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Re... 34 5.1
UniRef50_A6S5D0 Cluster: Putative uncharacterized protein; n=1; ... 34 5.1
UniRef50_P45434 Cluster: Translocon-associated protein subunit a... 34 5.1
UniRef50_Q93PA7 Cluster: MS124, putative transcriptional activat... 33 8.8
>UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2;
Endopterygota|Rep: Signal sequence receptor - Bombyx
mori (Silk moth)
Length = 279
Score = 180 bits (439), Expect = 3e-44
Identities = 84/84 (100%), Positives = 84/84 (100%)
Frame = +2
Query: 257 VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 436
VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP
Sbjct: 58 VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 117
Query: 437 MDYTYYIQNFTALPYNREVKPKQE 508
MDYTYYIQNFTALPYNREVKPKQE
Sbjct: 118 MDYTYYIQNFTALPYNREVKPKQE 141
Score = 126 bits (304), Expect = 7e-28
Identities = 59/63 (93%), Positives = 59/63 (93%)
Frame = +1
Query: 499 KTGTTFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 678
K TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF
Sbjct: 139 KQEATFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 198
Query: 679 LLY 687
LY
Sbjct: 199 FLY 201
Score = 57.6 bits (133), Expect = 4e-07
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = +3
Query: 87 MKLIYFTFLIVPAALLCLGSGNILARA 167
MKLIYFTFLIVPAALLCLGSGNILARA
Sbjct: 1 MKLIYFTFLIVPAALLCLGSGNILARA 27
>UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep:
CG32701-PA - Drosophila melanogaster (Fruit fly)
Length = 302
Score = 102 bits (244), Expect = 1e-20
Identities = 45/78 (57%), Positives = 61/78 (78%)
Frame = +2
Query: 266 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 445
SS + DT +LFTKP+ + G D+ G PVEF +GF NKG+ ++V+E++EASFRYPMD+
Sbjct: 64 SSPNADTYLLFTKPLYTPGQ-QLDLPGGKPVEFLIGFTNKGAEEFVIETVEASFRYPMDF 122
Query: 446 TYYIQNFTALPYNREVKP 499
Y+IQNF+A+ YNREVKP
Sbjct: 123 NYFIQNFSAVAYNREVKP 140
Score = 88.6 bits (210), Expect = 2e-16
Identities = 39/62 (62%), Positives = 50/62 (80%)
Frame = +1
Query: 508 TTFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLLY 687
+T +Y+F+P++ FAGRPFGLN+ L YRDA+G Y EAV+N+T+ I EV EGLDGETF LY
Sbjct: 144 STVSYTFLPSDQFAGRPFGLNIALAYRDANGIQYNEAVFNETVLISEVDEGLDGETFFLY 203
Query: 688 GL 693
L
Sbjct: 204 VL 205
>UniRef50_P43307 Cluster: Translocon-associated protein subunit
alpha precursor; n=51; Euteleostomi|Rep:
Translocon-associated protein subunit alpha precursor -
Homo sapiens (Human)
Length = 286
Score = 85.4 bits (202), Expect = 2e-15
Identities = 44/85 (51%), Positives = 57/85 (67%)
Frame = +2
Query: 254 GVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 433
G ++S DTTILF K G+ D A V+F VGF NKG+ D++VES++ASFRY
Sbjct: 75 GEPEASPSADTTILFVK-----GE---DFPANNIVKFLVGFTNKGTEDFIVESLDASFRY 126
Query: 434 PMDYTYYIQNFTALPYNREVKPKQE 508
P DY +YIQNFTALP N V P+++
Sbjct: 127 PQDYQFYIQNFTALPLNTVVPPQRQ 151
Score = 84.6 bits (200), Expect = 3e-15
Identities = 34/59 (57%), Positives = 45/59 (76%)
Frame = +1
Query: 511 TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLLY 687
TF YSFIP E GRPFGL + LNY+D +GN +Q+AV+NQT+ ++E +GLDGET +Y
Sbjct: 153 TFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMY 211
>UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 291
Score = 81.4 bits (192), Expect = 3e-14
Identities = 32/59 (54%), Positives = 46/59 (77%)
Frame = +1
Query: 511 TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLLY 687
+F Y+F P+E+F GRPFGL++ L Y+D G Y++AV+N+T+ +VE EG+DGETF LY
Sbjct: 158 SFEYAFRPHESFGGRPFGLSINLLYKDTDGKMYRDAVFNETVQLVESDEGMDGETFFLY 216
Score = 76.2 bits (179), Expect = 1e-12
Identities = 40/80 (50%), Positives = 46/80 (57%)
Frame = +2
Query: 260 VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPM 439
+K S D DT ILFTK AG + V F N G D++V+ MEAS RYP
Sbjct: 80 LKPSPDADTVILFTKNTEQ------QFSAGLVSKCLVSFSNNGRNDFMVDMMEASLRYPQ 133
Query: 440 DYTYYIQNFTALPYNREVKP 499
DY+YYIQNFTA YN VKP
Sbjct: 134 DYSYYIQNFTAFQYNTVVKP 153
>UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 257
Score = 74.5 bits (175), Expect = 3e-12
Identities = 33/76 (43%), Positives = 48/76 (63%)
Frame = +2
Query: 281 DTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQ 460
D + F PS ++ + G PV++ +GF NKG D+VV+ E SFR+P D++Y++Q
Sbjct: 48 DAGLAFHFVQPSDANVVREFYTGKPVKYLIGFQNKGEKDFVVKYAETSFRFPTDHSYHLQ 107
Query: 461 NFTALPYNREVKPKQE 508
NFT YNR V PK+E
Sbjct: 108 NFTRGEYNRRVAPKEE 123
Score = 68.9 bits (161), Expect = 1e-10
Identities = 36/88 (40%), Positives = 47/88 (53%)
Frame = +1
Query: 424 FPLSNGLHILHSELHRVAL*QRS*AKTGTTFAYSFIPNEAFAGRPFGLNVQLNYRDASGN 603
FP + H+ R +R K T Y F +E FAGRP GL V ++Y+DA GN
Sbjct: 98 FPTDHSYHL--QNFTRGEYNRRVAPKEEVTLDYGFYAHETFAGRPVGLVVNVHYQDADGN 155
Query: 604 AYQEAVYNQTLNIVEVSEGLDGETFLLY 687
+ VYNQT+NI+E G GET L+
Sbjct: 156 VFVNNVYNQTINIMEDDSGFSGETGFLF 183
>UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 247
Score = 71.3 bits (167), Expect = 3e-11
Identities = 35/83 (42%), Positives = 51/83 (61%)
Frame = +2
Query: 260 VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPM 439
+K+S D DTT LFT + G+ +I P++ VGF N G D+ +ES+ A+ RYP
Sbjct: 88 LKASPDADTTFLFT----TNGEK--EIPVTIPLQVLVGFTNNGQQDFTIESLHATLRYPQ 141
Query: 440 DYTYYIQNFTALPYNREVKPKQE 508
D+++YI NFT YN V+P +E
Sbjct: 142 DFSFYIYNFTEKEYNTVVEPNKE 164
>UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF7543,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 316
Score = 59.3 bits (137), Expect = 1e-07
Identities = 31/84 (36%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Frame = +1
Query: 439 GLHILHSELHRVAL*QRS*AKTGTTFAYSFIPNEAFAGRP-FGLNVQLNYRDASGNAYQE 615
GL +LH ELH + P A G L + LNY+D++GN +Q+
Sbjct: 159 GLPVLHPELHCSPAGNCGPSHATGHLRVLLHPRRAHGGGGRSALVINLNYKDSAGNVFQD 218
Query: 616 AVYNQTLNIVEVSEGLDGETFLLY 687
AV+NQT+ + E +GLDGET +Y
Sbjct: 219 AVFNQTVTVTEKEDGLDGETIFMY 242
Score = 55.6 bits (128), Expect = 1e-06
Identities = 32/61 (52%), Positives = 40/61 (65%)
Frame = +2
Query: 254 GVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 433
G VK+S DTTILF K G+ D A V+F +GF NKGS ++VVES++ASFRY
Sbjct: 82 GEVKASPHADTTILFVK-----GE---DFPANNIVKFLLGFTNKGSENFVVESLDASFRY 133
Query: 434 P 436
P
Sbjct: 134 P 134
>UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 236
Score = 48.8 bits (111), Expect = 2e-04
Identities = 22/58 (37%), Positives = 33/58 (56%)
Frame = +1
Query: 511 TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLL 684
T AY F+P+E R FGL + L Y+D + N + + +N T+NI E D ++F L
Sbjct: 111 TLAYRFVPSEYLEPREFGLLISLEYQDGAQN-FTQTFFNSTINITEKETSFDMDSFFL 167
Score = 35.5 bits (78), Expect = 1.7
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 344 AGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQNFT 469
AG VE VGF N + + AS +P D++ +IQN+T
Sbjct: 55 AGSVVEVLVGFTNNADKALNITHIFASLNHPQDFSVFIQNYT 96
>UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2;
Schistosoma japonicum|Rep: Clone ZZD1494 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 269
Score = 48.0 bits (109), Expect = 3e-04
Identities = 21/55 (38%), Positives = 31/55 (56%)
Frame = +1
Query: 520 YSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLL 684
Y F P+ AGR F L++ + Y D + Y ++NQT+N+ E EG+D E L
Sbjct: 141 YRFKPSPELAGRSFDLSIVVYYHDNNNIYYSHKLFNQTVNLFETEEGVDTELIFL 195
>UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole
genome shotgun sequence; n=8; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF9335,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 219
Score = 37.9 bits (84), Expect = 0.31
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 511 TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNA 606
TF SFIP E G PFGL + LNY D + A
Sbjct: 18 TFECSFIPAEPMGGAPFGLVINLNYEDGAVRA 49
>UniRef50_A0RNE9 Cluster: Peptidase, M48 family; n=2;
Campylobacter|Rep: Peptidase, M48 family - Campylobacter
fetus subsp. fetus (strain 82-40)
Length = 399
Score = 34.3 bits (75), Expect = 3.8
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +1
Query: 688 GLPWCWWSIGLSSQDSKLLRIIWPDVDSPRFNGLKPVENWEQPS 819
G W W+ GLS L+ +I+P + +P FN + P+ N E S
Sbjct: 162 GNSWWIWAFGLSFAIILLINLIYPTIIAPIFNKVTPLANEELKS 205
>UniRef50_A3JW84 Cluster: Sensor protein; n=2; Rhodobacterales
bacterium HTCC2150|Rep: Sensor protein - Rhodobacterales
bacterium HTCC2150
Length = 1521
Score = 33.9 bits (74), Expect = 5.1
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = +1
Query: 595 SGNAYQEAVYNQTLNIVEVS----EGLDGETFLLYGLPWCWWSIGLSSQDSKLLRIIW 756
S N +AVY+ LN++E S + ETF ++G +S+ SS D++LL W
Sbjct: 279 STNQIDDAVYHYALNVLESSTSNLSAVHSETFQIFGFDGHIYSVLKSSTDNQLLIFKW 336
>UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 249
Score = 33.9 bits (74), Expect = 5.1
Identities = 21/82 (25%), Positives = 39/82 (47%), Gaps = 1/82 (1%)
Frame = +2
Query: 266 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 445
++ D+++ + F P D F G V+ +G N G+ V + S P ++
Sbjct: 35 ANPDVESVVAF----PDHADRAFP--GGERVKVLLGLRNTGNDAVNVTHLAGSLNVPNNF 88
Query: 446 TYYIQNFTALPY-NREVKPKQE 508
+Y+ NFT + + +KPK+E
Sbjct: 89 NFYVTNFTVSSFGDAVIKPKKE 110
Score = 33.1 bits (72), Expect = 8.8
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = +1
Query: 499 KTGTTFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 678
K TF Y F + FAG L + Y D +G AY +N T+ +++ D E
Sbjct: 108 KKEATFEYEFAIDPQFAGHSLQLALTAFY-DEAGVAYATTYFNSTVPVLDPKVVFDRELA 166
Query: 679 LLY 687
++Y
Sbjct: 167 VIY 169
>UniRef50_A6S5D0 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 274
Score = 33.9 bits (74), Expect = 5.1
Identities = 17/37 (45%), Positives = 21/37 (56%)
Frame = +1
Query: 703 WWSIGLSSQDSKLLRIIWPDVDSPRFNGLKPVENWEQ 813
W ++SQ SK R IW D +SPR +G K WEQ
Sbjct: 115 WADAVIASQSSKTWRGIWNDHNSPR-DGRKTTTEWEQ 150
>UniRef50_P45434 Cluster: Translocon-associated protein subunit
alpha precursor; n=9; Magnoliophyta|Rep:
Translocon-associated protein subunit alpha precursor -
Arabidopsis thaliana (Mouse-ear cress)
Length = 258
Score = 33.9 bits (74), Expect = 5.1
Identities = 18/54 (33%), Positives = 23/54 (42%)
Frame = +2
Query: 338 IQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQNFTALPYNREVKP 499
+ AG E VG N+G V + AS P D+ +QN T L N P
Sbjct: 78 VPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLVQNLTMLRLNNASIP 131
Score = 33.9 bits (74), Expect = 5.1
Identities = 21/61 (34%), Positives = 27/61 (44%)
Frame = +1
Query: 511 TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFLLYG 690
TF Y F ++ F L + Y D G YQ YN T+ +VE L GE+ L
Sbjct: 137 TFPYIFAVSQYLQPGAFDLVGYIIY-DVEGKPYQSVFYNGTIEVVESGGLLSGESVFLLT 195
Query: 691 L 693
L
Sbjct: 196 L 196
>UniRef50_Q93PA7 Cluster: MS124, putative transcriptional activator;
n=1; Microscilla sp. PRE1|Rep: MS124, putative
transcriptional activator - Microscilla sp. PRE1
Length = 283
Score = 33.1 bits (72), Expect = 8.8
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 4/53 (7%)
Frame = +1
Query: 646 EVSEGLDGETFLL-YGLPWCWWSIGLSSQDSKLLRIIWPDV---DSPRFNGLK 792
++S+ +G+ +L LP CW + + +SK L I WP+ D P FNG+K
Sbjct: 53 DISDFKEGDLVMLGSNLPHCWKNDQEHTGESKSLVIQWPEKTIGDLPLFNGIK 105
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 804,537,099
Number of Sequences: 1657284
Number of extensions: 16107971
Number of successful extensions: 37709
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 36517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37703
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 72553824147
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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