BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30673 (717 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 151 1e-37 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 148 7e-37 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 73 4e-14 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 38 0.002 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 32 0.071 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.2 SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr... 28 1.5 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5 SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 27 2.0 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 27 3.5 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.5 SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce... 26 4.7 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.7 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.2 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 6.2 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 6.2 SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces po... 25 8.2 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 151 bits (366), Expect = 1e-37 Identities = 66/87 (75%), Positives = 74/87 (85%) Frame = +1 Query: 1 VDLTEFQTNLVPYSRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRH 180 VDL EFQTNLVPY RIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR Sbjct: 250 VDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRA 309 Query: 181 GKYMACCMLYRGDVVPKDVNAAIATSK 261 G+YMA C+LYRGDV+P+DV AA+ T K Sbjct: 310 GRYMATCLLYRGDVIPRDVQAAVTTIK 336 Score = 138 bits (333), Expect = 1e-33 Identities = 59/75 (78%), Positives = 67/75 (89%) Frame = +3 Query: 255 IKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHK 434 IK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHK Sbjct: 335 IKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHK 394 Query: 435 FDLMYAKRAFVHWYV 479 FDLMY+KRAFVHWYV Sbjct: 395 FDLMYSKRAFVHWYV 409 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 148 bits (359), Expect = 7e-37 Identities = 64/87 (73%), Positives = 74/87 (85%) Frame = +1 Query: 1 VDLTEFQTNLVPYSRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRH 180 VDL EFQTNLVPY RIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR Sbjct: 254 VDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRT 313 Query: 181 GKYMACCMLYRGDVVPKDVNAAIATSK 261 G+YMA C+LYRGDV+P+DV AA+ + K Sbjct: 314 GRYMATCLLYRGDVIPRDVQAAVTSIK 340 Score = 144 bits (348), Expect = 1e-35 Identities = 61/75 (81%), Positives = 69/75 (92%) Frame = +3 Query: 255 IKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHK 434 IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHK Sbjct: 339 IKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHK 398 Query: 435 FDLMYAKRAFVHWYV 479 FDLMY+KRAFVHWYV Sbjct: 399 FDLMYSKRAFVHWYV 413 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 72.9 bits (171), Expect = 4e-14 Identities = 32/82 (39%), Positives = 50/82 (60%) Frame = +1 Query: 4 DLTEFQTNLVPYSRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHG 183 DL + N+VP+ R+HF +V +AP+ + + + +SV E+T F+ N MV DPRHG Sbjct: 249 DLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHG 308 Query: 184 KYMACCMLYRGDVVPKDVNAAI 249 +Y+ L+RG V K+V+ I Sbjct: 309 RYLTVAALFRGKVSMKEVDEQI 330 Score = 47.2 bits (107), Expect = 2e-06 Identities = 24/76 (31%), Positives = 40/76 (52%) Frame = +3 Query: 249 RYIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLD 428 R ++TK + FV+W P + PP DL + + + N+T+I E + RL Sbjct: 331 RSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLG 382 Query: 429 HKFDLMYAKRAFVHWY 476 +F M+ ++AF+HWY Sbjct: 383 DQFSAMFRRKAFLHWY 398 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 37.5 bits (83), Expect = 0.002 Identities = 22/86 (25%), Positives = 41/86 (47%), Gaps = 4/86 (4%) Frame = +1 Query: 4 DLTEFQTNLVPYSRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP 174 DL +L+P R HF L +Y P + E + +V ++ P NQMV +P Sbjct: 252 DLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311 Query: 175 -RHGKYMACCMLYRGDVVPKDVNAAI 249 + +++ + +G+ P DV+ ++ Sbjct: 312 SKKSCFISILDIIQGEADPADVHKSL 337 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 32.3 bits (70), Expect = 0.071 Identities = 21/74 (28%), Positives = 35/74 (47%) Frame = -1 Query: 255 CSDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGKLFMVGLLGRDD 76 CS G +LGY ++ A++V+A + V HL+ G D + K G D Sbjct: 406 CSAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK-------GLDM 458 Query: 75 WRVRDQWEVDTGVR 34 R Q+E++ G++ Sbjct: 459 LVDRVQYELEAGLQ 472 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 1.2 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = +1 Query: 124 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 249 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 27.9 bits (59), Expect = 1.5 Identities = 19/79 (24%), Positives = 36/79 (45%) Frame = -3 Query: 580 LQRSSMPTFFVILLEGSQVPHGASKNSPSSIPSPTYQCTKARLAYMRSNLWSRRAQASAM 401 L S MP+ F L S + N PS+IP+P+ + A + +L + + S+ Sbjct: 134 LPPSEMPSHFHPLWSKSASKDSNAFNDPSAIPNPSPLFSSAYATRINGSLRNDKWNRSSF 193 Query: 400 AVVLDNMQTAR*TLAKSPP 344 + L + + +R + + P Sbjct: 194 SEALSSSRFSRPQVGQQQP 212 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +2 Query: 11 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 157 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPBC1539.09c |trp1||anthranilate synthase component II|Schizosaccharomyces pombe|chr 2|||Manual Length = 759 Score = 27.5 bits (58), Expect = 2.0 Identities = 15/45 (33%), Positives = 25/45 (55%) Frame = +1 Query: 163 KCDPRHGKYMACCMLYRGDVVPKDVNAAIATSKPSVLSNSSTGVQ 297 K D ++ + G + PK+V AI+ S+P+V+ SS GV+ Sbjct: 696 KVDWEAASFIPVSYVLAGGLTPKNVQDAISVSRPAVVDVSS-GVE 739 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Frame = +1 Query: 217 DVVPKDVNAAIATSKPSVLSNSST--GVQPVSRSVSTTSHPPW 339 D +D + I+ S+P+++SN ST G+Q V V + W Sbjct: 522 DYAIRDFDTPISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 3.5 Identities = 16/41 (39%), Positives = 23/41 (56%) Frame = +1 Query: 238 NAAIATSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWP 360 +++ ATS S S SS+ V+ S ST S PP P ++P Sbjct: 661 SSSTATSSTSFASESSSFYSNVTTSSSTVSTPP--PTTSFP 699 Score = 26.2 bits (55), Expect = 4.7 Identities = 20/77 (25%), Positives = 36/77 (46%), Gaps = 2/77 (2%) Frame = +2 Query: 5 TSPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPV 178 TS S L+ + +++ T S+ P PT+ S +P + T+ ++ + +TPV Sbjct: 381 TSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPV 440 Query: 179 MASTWLAVCCTVVTSYP 229 ++ CT TS P Sbjct: 441 TSTPLATTNCTTSTSVP 457 >SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 855 Score = 26.2 bits (55), Expect = 4.7 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 241 AAIATSKPSVLSNSSTGVQPVSRSVSTTSH 330 AAI+ + SV+SN+S P R++ + SH Sbjct: 568 AAISARRSSVISNASIETGPSLRNLKSLSH 597 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.7 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 74 QSSLPRRPTMNSFPSPRSQTHASSPPTR 157 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 6.2 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +2 Query: 8 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 148 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 6.2 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -3 Query: 229 WVRRHHGTAYSKPCTCHDGG 170 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 6.2 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = +2 Query: 35 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 187 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC1296.06 |||NADPH cytochrome reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 25.4 bits (53), Expect = 8.2 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = +1 Query: 217 DVVPKDVNAAIATSKPSVLSNSSTGVQPV 303 D++P +N + ++KP ++ TGV P+ Sbjct: 399 DILPGFLNLSYQSNKPLIMVGPGTGVAPL 427 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,291,226 Number of Sequences: 5004 Number of extensions: 71740 Number of successful extensions: 263 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 242 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 262 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 335201398 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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