BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30670 (664 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 109 3e-25 SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |... 27 2.4 SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomy... 26 4.2 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 26 4.2 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 26 5.6 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 26 5.6 SPAC5D6.05 |sep11|pmc6, med18|mediator complex subunit Pmc6 |Sch... 25 7.4 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 7.4 SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 25 7.4 SPCC191.01 ||SPCC417.13|sequence orphan|Schizosaccharomyces pomb... 25 9.7 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 109 bits (263), Expect = 3e-25 Identities = 65/117 (55%), Positives = 73/117 (62%) Frame = +3 Query: 117 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIVAK 296 + ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIR K Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSLSDPHALDAEK 61 Query: 297 SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGADISMI 467 R P + +L +G K L LG IAKSGTK FMEA +GADISMI Sbjct: 62 DLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAAASGADISMI 118 >SPCC895.06 |||RNA polymerase II elongator complex subunit Elp2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 760 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/27 (44%), Positives = 15/27 (55%), Gaps = 4/27 (14%) Frame = +1 Query: 379 FWGQFWEPSRNLVL----KLSWRLFKQ 447 FWG W P+ N V+ WRL+KQ Sbjct: 329 FWGGLWNPNGNCVVCWGRTGGWRLWKQ 355 >SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 357 Score = 26.2 bits (55), Expect = 4.2 Identities = 16/40 (40%), Positives = 20/40 (50%) Frame = +3 Query: 171 IINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIV 290 II+TF KE L +I D L +R N R+N IV Sbjct: 198 IIDTFLQEKEPSLHVIIEPHPDVLKHMRKNGWMDRENVIV 237 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 26.2 bits (55), Expect = 4.2 Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 6/61 (9%) Frame = +2 Query: 269 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKGDFGDNFG------NHREIWY*SFH 430 DPSK ++Y K+ + + L D+GI ++ + G N +E+ Y +FH Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIGISLVERNTQELAYLTFH 2531 Query: 431 G 433 G Sbjct: 2532 G 2532 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.8 bits (54), Expect = 5.6 Identities = 14/64 (21%), Positives = 32/64 (50%) Frame = +3 Query: 90 MPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSR 269 + E++ + A+ E+F A+L +N NKE + +L + S+ +++ S+ Sbjct: 762 LTEKLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQ 821 Query: 270 IRQN 281 + Q+ Sbjct: 822 LNQD 825 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.8 bits (54), Expect = 5.6 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 531 NSKTPLTTSK*RGGDLSARRFRFTVPAQNKPVKPPLGPKGTK 656 NS PL+T G + + T + N+P KPP+ TK Sbjct: 58 NSDVPLSTLVSSGQQILVLKNAATSFSTNEPAKPPIPNAATK 99 >SPAC5D6.05 |sep11|pmc6, med18|mediator complex subunit Pmc6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 207 Score = 25.4 bits (53), Expect = 7.4 Identities = 8/24 (33%), Positives = 15/24 (62%) Frame = +3 Query: 573 DLSARRFRFTVPAQNKPVKPPLGP 644 D + R ++ +P+Q + +KPP P Sbjct: 135 DTTVRIYQTLIPSQQRSIKPPFHP 158 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 25.4 bits (53), Expect = 7.4 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 1/56 (1%) Frame = +3 Query: 189 SNKEIFLRELISNSSDALDKIRMNLSRIRQNSI-VAKSCTSRSFPTRTRALLRSSI 353 S+ +L +L+++ D L K+R RIR+ I V S T TRA+++ + Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECL 380 >SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 25.4 bits (53), Expect = 7.4 Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 3/27 (11%) Frame = -2 Query: 414 QISRW---FPKLSPKSPLVIPIPVSMI 343 Q S W F KL+PK+ ++ PIP +++ Sbjct: 14 QFSSWYSLFRKLTPKAKVIKPIPATVL 40 >SPCC191.01 ||SPCC417.13|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 178 Score = 25.0 bits (52), Expect = 9.7 Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 1/45 (2%) Frame = +2 Query: 503 TLSLTA*PFQL*-NTIDDEQITWWGSFCKEVPFHSSGPEQAGEAP 634 T S T+ P +L +++D +I W FC V + P + EAP Sbjct: 103 TSSATSTPKRLSISSMDPARIHLWRQFCNVVGYDMPTPGEQKEAP 147 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,897,064 Number of Sequences: 5004 Number of extensions: 64260 Number of successful extensions: 170 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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