Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30670
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 126 1e-29
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 121 4e-28
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 121 4e-28
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 121 4e-28
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 91 8e-19
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 91 8e-19
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 89 2e-18
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 81 6e-16
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 81 6e-16
At3g51350.1 68416.m05622 aspartyl protease family protein contai... 29 2.8
At5g62100.2 68418.m07795 BAG domain-containing protein similar t... 28 4.8
At5g62100.1 68418.m07794 BAG domain-containing protein similar t... 28 4.8
At5g52060.1 68418.m06462 BAG domain-containing protein contains ... 28 6.4
At1g71770.1 68414.m08295 polyadenylate-binding protein 5 (PABP5)... 28 6.4
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 126 bits (304), Expect = 1e-29
Identities = 72/121 (59%), Positives = 81/121 (66%)
Frame = +3
Query: 105 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNS 284
+ Q A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR +
Sbjct: 3 DVQMADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKL 62
Query: 285 IVAKSCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGADISM 464
R P ++ L +G KA L LGTIA+SGTK FMEALQAGAD+SM
Sbjct: 63 DGQPELFIRLVPDKSNKTLSIIDSGIGMTKADLVNNLGTIARSGTKEFMEALQAGADVSM 122
Query: 465 I 467
I
Sbjct: 123 I 123
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 121 bits (292), Expect = 4e-28
Identities = 70/117 (59%), Positives = 77/117 (65%)
Frame = +3
Query: 117 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIVAK 296
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR +
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 297 SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGADISMI 467
P +T L +G KA L LGTIA+SGTK FMEAL AGAD+SMI
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMI 118
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 121 bits (292), Expect = 4e-28
Identities = 70/117 (59%), Positives = 77/117 (65%)
Frame = +3
Query: 117 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIVAK 296
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR +
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 297 SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGADISMI 467
P +T L +G KA L LGTIA+SGTK FMEAL AGAD+SMI
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMI 118
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 121 bits (292), Expect = 4e-28
Identities = 70/117 (59%), Positives = 77/117 (65%)
Frame = +3
Query: 117 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIVAK 296
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR +
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 297 SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGADISMI 467
P +T L +G KA L LGTIA+SGTK FMEAL AGAD+SMI
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMI 118
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 90.6 bits (215), Expect = 8e-19
Identities = 53/125 (42%), Positives = 78/125 (62%), Gaps = 4/125 (3%)
Frame = +3
Query: 105 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNS 284
+T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR +
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIR--FLALTDKD 127
Query: 285 IVAKSCTSR----SFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGA 452
++ + T++ + + +L +G K L LGTIAKSGT AF+E +Q+
Sbjct: 128 VLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSG 187
Query: 453 DISMI 467
D+++I
Sbjct: 188 DLNLI 192
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 90.6 bits (215), Expect = 8e-19
Identities = 53/125 (42%), Positives = 78/125 (62%), Gaps = 4/125 (3%)
Frame = +3
Query: 105 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNS 284
+T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR +
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIR--FLALTDKD 127
Query: 285 IVAKSCTSR----SFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAGA 452
++ + T++ + + +L +G K L LGTIAKSGT AF+E +Q+
Sbjct: 128 VLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSG 187
Query: 453 DISMI 467
D+++I
Sbjct: 188 DLNLI 192
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 89.4 bits (212), Expect = 2e-18
Identities = 68/185 (36%), Positives = 98/185 (52%), Gaps = 4/185 (2%)
Frame = +3
Query: 114 PAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIRQNSIVA 293
P E F +QAE+++LM LI+N+ YSNKE+FLRELISN+SDALDK+R + S A
Sbjct: 91 PPPAEKFEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRYLSVTNPELSKDA 150
Query: 294 KSCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQ----AGADIS 461
R + + ++ + +G + L LGTIA+SGT FM+AL+ AG D +
Sbjct: 151 PDLDIRIYADKENGIITLTDSGIGMTRQELVDCLGTIAQSGTAKFMKALKDSKDAGGDNN 210
Query: 462 MIWTVSVLAF*SNLLCR*RRDRFNSKTPLTTSK*RGGDLSARRFRFTVPAQNKPVKPPLG 641
+I V F S L R ++K+P + K + A FT+ P L
Sbjct: 211 LIGQFGV-GFYSAFLVA-DRVIVSTKSP-KSDKQYVWEGEANSSSFTIQEDTDP--QSLI 265
Query: 642 PKGTK 656
P+GT+
Sbjct: 266 PRGTR 270
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 81.0 bits (191), Expect = 6e-16
Identities = 48/123 (39%), Positives = 75/123 (60%), Gaps = 2/123 (1%)
Frame = +3
Query: 96 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIR 275
E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R LS +
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRF-LS-VT 124
Query: 276 QNSIVAK--SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAG 449
+ S++ R P + + +G K L LGTIA+SGT F++AL+
Sbjct: 125 EPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKALKEN 184
Query: 450 ADI 458
D+
Sbjct: 185 KDL 187
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 81.0 bits (191), Expect = 6e-16
Identities = 48/123 (39%), Positives = 75/123 (60%), Gaps = 2/123 (1%)
Frame = +3
Query: 96 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRMNLSRIR 275
E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R LS +
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRF-LS-VT 124
Query: 276 QNSIVAK--SCTSRSFPTRTRALLRSSIPVLG*PKAILGTILGTIAKSGTKAFMEALQAG 449
+ S++ R P + + +G K L LGTIA+SGT F++AL+
Sbjct: 125 EPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKALKEN 184
Query: 450 ADI 458
D+
Sbjct: 185 KDL 187
>At3g51350.1 68416.m05622 aspartyl protease family protein contains
Eukaryotic and viral aspartyl proteases active site,
PROSITE:PS00141
Length = 528
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = -1
Query: 364 PNTGIDDRKSALVLVGNDLDVQLFATIE----F*RIRERFILILSKASDELEISSRRKI 200
P+T S + + G+ +D++LFA + F +RE +L+K+ DEL RR +
Sbjct: 301 PSTAYGVNISGVSVAGDPVDIRLFAKFDTGSSFTHLREPAYGVLTKSFDELVEDRRRPV 359
>At5g62100.2 68418.m07795 BAG domain-containing protein similar to
BAG domain containing proteins (At5g07220, At5g52060)
Length = 285
Score = 28.3 bits (60), Expect = 4.8
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = -1
Query: 358 TGIDDRKSALVLVGNDLDVQLFATIEF*RIRERFILILSKASDELEISSRRKISLLE*KV 179
TG+ + ++ + D ++F + + R + ILI S E + RKI+ E
Sbjct: 71 TGVHHQDMQIIYKDKERDSKMFLDLSGVKDRSKLILIEDPISQEKRLLELRKIATKEKSS 130
Query: 178 LMIRDIS 158
I DIS
Sbjct: 131 KAISDIS 137
>At5g62100.1 68418.m07794 BAG domain-containing protein similar to
BAG domain containing proteins (At5g07220, At5g52060)
Length = 296
Score = 28.3 bits (60), Expect = 4.8
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = -1
Query: 358 TGIDDRKSALVLVGNDLDVQLFATIEF*RIRERFILILSKASDELEISSRRKISLLE*KV 179
TG+ + ++ + D ++F + + R + ILI S E + RKI+ E
Sbjct: 71 TGVHHQDMQIIYKDKERDSKMFLDLSGVKDRSKLILIEDPISQEKRLLELRKIATKEKSS 130
Query: 178 LMIRDIS 158
I DIS
Sbjct: 131 KAISDIS 137
>At5g52060.1 68418.m06462 BAG domain-containing protein contains
Pfam:PF02179 BAG domain
Length = 326
Score = 27.9 bits (59), Expect = 6.4
Identities = 20/78 (25%), Positives = 32/78 (41%)
Frame = -1
Query: 358 TGIDDRKSALVLVGNDLDVQLFATIEF*RIRERFILILSKASDELEISSRRKISLLE*KV 179
TGI + L+ + D + F + + + + +LI S E RKI+ E
Sbjct: 83 TGIHHQDQKLMYKDKERDSKAFLDVSGVKDKSKMVLIEDPLSQEKRFLEMRKIAKTEKAS 142
Query: 178 LMIRDIS*AISAWNAKVS 125
I DIS + +VS
Sbjct: 143 KAISDISLEVDRLGGRVS 160
>At1g71770.1 68414.m08295 polyadenylate-binding protein 5 (PABP5)
identical to GB:Q05196 from [Arabidopsis thaliana]
Length = 668
Score = 27.9 bits (59), Expect = 6.4
Identities = 15/54 (27%), Positives = 27/54 (50%)
Frame = +2
Query: 227 FIGRFRQNQDESLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKGDFGD 388
F+G F + QD + ++ + S +Y+K +P + I D + T G +GD
Sbjct: 203 FVGHFVRRQDRARSESGAVPSFTNVYVKNLPKE------ITDDELKKTFGKYGD 250
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,418,520
Number of Sequences: 28952
Number of extensions: 351870
Number of successful extensions: 1005
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 987
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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