Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30663
(711 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 219 2e-57
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 214 5e-56
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 180 1e-45
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 168 4e-42
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 90 1e-18
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 82 4e-16
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 67 1e-11
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 56 3e-08
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 40 0.002
SB_24060| Best HMM Match : GED (HMM E-Value=0.52) 31 0.70
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 30 2.1
SB_57708| Best HMM Match : Aldedh (HMM E-Value=0) 29 4.9
SB_23595| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_26917| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 28 6.5
SB_47949| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.6
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 219 bits (535), Expect = 2e-57
Identities = 123/199 (61%), Positives = 140/199 (70%), Gaps = 1/199 (0%)
Frame = +2
Query: 2 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQVAMNPNNHNIRCQTSH 181
YSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DT RLIGDAAKNQVAMNP N +
Sbjct: 15 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVAMNPENTVFDAKRLI 74
Query: 182 RT*VRRCYCASRHEALAFRGCQ*WSKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAY 361
A F + +PKI+V +KGE K+FFPEE+SSMVLTKMKETAEAY
Sbjct: 75 GRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSMVLTKMKETAEAY 134
Query: 362 LGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLR-IHQ*NXXXXXXXXS*QKGYWRN 538
LG V +AV+TVPAYF+DSQRQATKDAGTI+GLNVLR I++ + G RN
Sbjct: 135 LGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGVERN 194
Query: 539 EIVLIFDLGRAVPFDVSIL 595
VLI+DLG FDVS+L
Sbjct: 195 --VLIYDLGGGT-FDVSVL 210
Score = 59.3 bits (137), Expect = 3e-09
Identities = 25/53 (47%), Positives = 34/53 (64%)
Frame = +3
Query: 156 TIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGANLRSR*HIRVKTKPFSPRK 314
T+FDAKRLIGRKF+D V DM HWPF V+ +G + + + + K F P +
Sbjct: 66 TVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEE 118
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 214 bits (523), Expect = 5e-56
Identities = 124/206 (60%), Positives = 139/206 (67%)
Frame = +2
Query: 2 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQVAMNPNNHNIRCQTSH 181
YSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDT RL+GDAAKNQVA+NP N +
Sbjct: 15 YSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVALNPENTIFDAKRLI 74
Query: 182 RT*VRRCYCASRHEALAFRGCQ*WSKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAY 361
S + F+ +KPKI+V YKGE KTF EE+SSMVL KMKETAEAY
Sbjct: 75 GRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSMVLGKMKETAEAY 134
Query: 362 LGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIHQ*NXXXXXXXXS*QKGYWRNE 541
LG+ V +AVITVPAYFNDSQRQATKDAGTI+GLNVLR+ K +
Sbjct: 135 LGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVIN-EPTAAALAYGLDKSLSGEK 193
Query: 542 IVLIFDLGRAVPFDVSIL*PSRDGNL 619
VLIFDLG FDVSIL DG+L
Sbjct: 194 NVLIFDLGGGT-FDVSIL-TIDDGSL 217
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 180 bits (438), Expect = 1e-45
Identities = 105/199 (52%), Positives = 128/199 (64%), Gaps = 1/199 (0%)
Frame = +2
Query: 2 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT*-RLIGDAAKNQVAMNPNNHNIRCQTS 178
YSCVGVF++G+VEIIANDQGNR TPSYVAFT+ RLIGD AKNQ+ NP N +
Sbjct: 712 YSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPENTVFDAKRL 771
Query: 179 HRT*VRRCYCASRHEALAFRGCQ*WSKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEA 358
+ + F+ KP ++V K E KTF EE+S+MVLTKMKETAEA
Sbjct: 772 IGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAMVLTKMKETAEA 831
Query: 359 YLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIHQ*NXXXXXXXXS*QKGYWRN 538
YLGK V +AV+TVPAYFND+QRQATKDAG I+GL V+RI +K +N
Sbjct: 832 YLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKKEGEKN 891
Query: 539 EIVLIFDLGRAVPFDVSIL 595
+L+FDLG FDVS+L
Sbjct: 892 --ILVFDLGGGT-FDVSLL 907
Score = 48.8 bits (111), Expect = 4e-06
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = +3
Query: 156 TIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGANLRSR*HIRVKTK 296
T+FDAKRLIGR ++D +VQ D+K +PF+VV + + H++VK K
Sbjct: 764 TVFDAKRLIGRSWDDTSVQNDLKFFPFKVV----DKNKKPHVQVKVK 806
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 168 bits (408), Expect = 4e-42
Identities = 100/173 (57%), Positives = 108/173 (62%), Gaps = 25/173 (14%)
Frame = +2
Query: 2 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQVAMNPNNHNIRCQTSH 181
YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD RL+GDAAKNQVAMNP N +
Sbjct: 15 YSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVAMNPTNTIFDAKRLI 74
Query: 182 RT*VRRCYCASRHEALAFRGCQ*WSKPKIKVAYKGEDKTFFPEEV--------------- 316
+ +F +PK+KV YKGE KTFF EEV
Sbjct: 75 GRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNEAGRPKVKVEYKG 134
Query: 317 ----------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 445
SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG
Sbjct: 135 ETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAG 187
Score = 104 bits (250), Expect = 6e-23
Identities = 61/96 (63%), Positives = 69/96 (71%), Gaps = 1/96 (1%)
Frame = +2
Query: 311 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLR-IHQ*N 487
++SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLR I++
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 488 XXXXXXXXS*QKGYWRNEIVLIFDLGRAVPFDVSIL 595
+ G RN VLIFDLG FDVS+L
Sbjct: 271 AAAIAYGLDKKVGQERN--VLIFDLGGGT-FDVSVL 303
Score = 58.4 bits (135), Expect = 5e-09
Identities = 26/49 (53%), Positives = 35/49 (71%)
Frame = +3
Query: 156 TIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGANLRSR*HIRVKTKPF 302
TIFDAKRLIGR+F+D V+ DMKHW FEVV++ + + + +TK F
Sbjct: 66 TIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTF 114
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 90.2 bits (214), Expect = 1e-18
Identities = 42/68 (61%), Positives = 57/68 (83%)
Frame = +2
Query: 266 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 445
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAG
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 446 TISGLNVL 469
TI+GL+VL
Sbjct: 62 TIAGLDVL 69
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 82.2 bits (194), Expect = 4e-16
Identities = 52/161 (32%), Positives = 80/161 (49%), Gaps = 3/161 (1%)
Frame = +2
Query: 2 YSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQVAMNPNNHNIRCQT 175
YSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q +NP N +
Sbjct: 42 YSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAEINPKNTIYDAKR 101
Query: 176 SHRT*VRRCYCASRHEALAFR-GCQ*WSKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETA 352
+ + FR V KG PEE+ V+ K++ A
Sbjct: 102 FIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGGYVIKKLRTMA 161
Query: 353 EAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRI 475
E L TV AV++VPA F+ QR AT A +++G+ VLR+
Sbjct: 162 ERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRV 202
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 67.3 bits (157), Expect = 1e-11
Identities = 31/70 (44%), Positives = 46/70 (65%)
Frame = +2
Query: 266 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 445
I+V Y G+ + F PE+V +M+ T++K TAE L V + VI+VP+Y+ D QR+ DA
Sbjct: 160 IQVQYLGKQEVFSPEQVMAMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDAS 219
Query: 446 TISGLNVLRI 475
+GLN LR+
Sbjct: 220 ATAGLNCLRL 229
Score = 41.1 bits (92), Expect = 9e-04
Identities = 20/44 (45%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +2
Query: 5 SC-VGVFQHGKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQ 133
SC + V + G +E +AN+ +R TPS+V+ D RLIG + KNQ
Sbjct: 12 SCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 36.7 bits (81), Expect = 0.019
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +3
Query: 156 TIFDAKRLIGRKFEDATVQADMKHWPFEVV 245
TI KR IGRKF D VQ ++ H P++VV
Sbjct: 122 TISQFKRFIGRKFSDPAVQKEIPHLPYKVV 151
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 56.0 bits (129), Expect = 3e-08
Identities = 27/72 (37%), Positives = 43/72 (59%)
Frame = +2
Query: 260 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKD 439
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN ++R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 440 AGTISGLNVLRI 475
A + GLNVL+I
Sbjct: 127 AAELVGLNVLQI 138
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 40.3 bits (90), Expect = 0.002
Identities = 20/46 (43%), Positives = 26/46 (56%)
Frame = +2
Query: 29 GKVEIIANDQGNRTTPSYVAFTDT*RLIGDAAKNQVAMNPNNHNIR 166
G IIAND G+R TP+ VAF+DT +LI + N NI+
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQ 128
>SB_24060| Best HMM Match : GED (HMM E-Value=0.52)
Length = 297
Score = 31.5 bits (68), Expect = 0.70
Identities = 27/89 (30%), Positives = 42/89 (47%)
Frame = -3
Query: 574 YRPPEVKDKYNFVPPVPFLSRPKQSQQQSVSLMNSENVQARDGTCIFCGLSLRVIEVRGN 395
++P E K K VP + QQ ++ N EN+++ T IFCG + I +RG
Sbjct: 40 WKPEEFK-KMMRTDAVPINLQLNNIVQQQIA-RNRENLRSLFKTIIFCGKN--NIPLRGL 95
Query: 394 RDNCILHSFAKISFGSFLHFRKHHGTDFL 308
RD+ ++ +F + L FR G L
Sbjct: 96 RDSDPTNAALAGNFQALLEFRVDSGDQIL 124
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 29.9 bits (64), Expect = 2.1
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 38 EIIANDQGNRTTPSYVAFT 94
++I N +G+RTTPS VAFT
Sbjct: 7 KVIENAEGSRTTPSVVAFT 25
>SB_57708| Best HMM Match : Aldedh (HMM E-Value=0)
Length = 528
Score = 28.7 bits (61), Expect = 4.9
Identities = 17/45 (37%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +1
Query: 88 VHRHIAS--HRRCRQEPGGDE-PQQSQYSMPNVSSDVSSKMLLCK 213
V RH+A + + GG PQ + P V +DVS ML+CK
Sbjct: 337 VERHVADALSKGAKLMRGGHRHPQGENFFEPTVLTDVSPDMLVCK 381
>SB_23595| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 281
Score = 28.7 bits (61), Expect = 4.9
Identities = 21/73 (28%), Positives = 37/73 (50%), Gaps = 4/73 (5%)
Frame = -3
Query: 634 WISPSKITIPRWLKDGHVERYRPPEVKDKYNFVPPVPFLSR----PKQSQQQSVSLMNSE 467
W+S KI + R+ +G++ R + K+ +PP+P LSR P+ S + + +
Sbjct: 37 WVSAKKI-VDRY--EGNINRDKG-RAKNSVR-LPPLPCLSRFKEKPENSNIEGAKKKHRQ 91
Query: 466 NVQARDGTCIFCG 428
V+ R I+CG
Sbjct: 92 EVKIRPSEDIYCG 104
>SB_26917| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 601
Score = 28.7 bits (61), Expect = 4.9
Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
Frame = -3
Query: 640 AGWISPSKITIPRWLKDGHV--ERYRPP 563
+GW + S I +P WL+D + +RPP
Sbjct: 216 SGWYTLSHIELPHWLRDNEFLEDSHRPP 243
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 28.3 bits (60), Expect = 6.5
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = -3
Query: 688 CFKKSLNLPKSWGCPPAGW 632
C + S +SWGCPP W
Sbjct: 130 CKRSSQEQDESWGCPPGFW 148
>SB_47949| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 280
Score = 27.9 bits (59), Expect = 8.6
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 675 PSTFPKVGVVPRPGGFHPRRLP 610
P P G++P PGG P R+P
Sbjct: 213 PGLMPPPGMLPPPGGMPPGRMP 234
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,061,880
Number of Sequences: 59808
Number of extensions: 567769
Number of successful extensions: 1400
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1297
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1394
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1877743452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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