Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30658
(566 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 129 1e-30
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 129 1e-30
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 128 3e-30
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 126 1e-29
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 69 3e-12
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 67 9e-12
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 65 4e-11
At3g59510.1 68416.m06641 leucine-rich repeat family protein cont... 28 5.0
At5g06420.2 68418.m00719 zinc finger (CCCH-type/C3HC4-type RING ... 27 8.7
At5g06420.1 68418.m00718 zinc finger (CCCH-type/C3HC4-type RING ... 27 8.7
At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1) (... 27 8.7
At1g24350.1 68414.m03071 expressed protein contains Pfam domain,... 27 8.7
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 129 bits (312), Expect = 1e-30
Identities = 62/85 (72%), Positives = 72/85 (84%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKLTGMAFRVP +NVSVVDLT RL K ASYE +K +K A+EGPLKGILGYT++ VVS+D
Sbjct: 310 GKLTGMAFRVPTSNVSVVDLTCRLEKGASYEDVKAAIKHASEGPLKGILGYTDEDVVSND 369
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F+GDS SSIFDA AGI L+ +FVKL
Sbjct: 370 FVGDSRSSIFDANAGIGLSKSFVKL 394
Score = 40.7 bits (91), Expect = 7e-04
Identities = 14/21 (66%), Positives = 21/21 (100%)
Frame = +3
Query: 255 ISWYDNEYGYSSRVIDLIKYI 317
+SWYDNE+GYS+RV+DLI+++
Sbjct: 395 VSWYDNEWGYSNRVLDLIEHM 415
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 129 bits (311), Expect = 1e-30
Identities = 61/85 (71%), Positives = 73/85 (85%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKLTGM+FRVP +VSVVDLTVRL K A+Y+ IK+ +KE +EG LKGILGYTED VVS+D
Sbjct: 230 GKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEGKLKGILGYTEDDVVSTD 289
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F+GD+ SSIFDA AGI+L+D FVKL
Sbjct: 290 FVGDNRSSIFDAKAGIALSDKFVKL 314
Score = 41.1 bits (92), Expect = 5e-04
Identities = 15/21 (71%), Positives = 20/21 (95%)
Frame = +3
Query: 255 ISWYDNEYGYSSRVIDLIKYI 317
+SWYDNE+GYSSRV+DLI ++
Sbjct: 315 VSWYDNEWGYSSRVVDLIVHM 335
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 128 bits (309), Expect = 3e-30
Identities = 60/85 (70%), Positives = 73/85 (85%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKLTGM+FRVP +VSVVDLTVRL K A+Y+ IK+ +KE +EG +KGILGYTED VVS+D
Sbjct: 230 GKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEGKMKGILGYTEDDVVSTD 289
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F+GD+ SSIFDA AGI+L+D FVKL
Sbjct: 290 FVGDNRSSIFDAKAGIALSDKFVKL 314
Score = 41.1 bits (92), Expect = 5e-04
Identities = 15/21 (71%), Positives = 20/21 (95%)
Frame = +3
Query: 255 ISWYDNEYGYSSRVIDLIKYI 317
+SWYDNE+GYSSRV+DLI ++
Sbjct: 315 VSWYDNEWGYSSRVVDLIVHM 335
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 126 bits (304), Expect = 1e-29
Identities = 61/85 (71%), Positives = 71/85 (83%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKLTGMAFRVP NVSVVDLT RL K ASYE +K +K A+EGPL+GILGYTE+ VVS+D
Sbjct: 308 GKLTGMAFRVPTPNVSVVDLTCRLEKDASYEDVKAAIKFASEGPLRGILGYTEEDVVSND 367
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F+GDS SSIFDA AGI L+ +F+KL
Sbjct: 368 FLGDSRSSIFDANAGIGLSKSFMKL 392
Score = 40.7 bits (91), Expect = 7e-04
Identities = 14/21 (66%), Positives = 21/21 (100%)
Frame = +3
Query: 255 ISWYDNEYGYSSRVIDLIKYI 317
+SWYDNE+GYS+RV+DLI+++
Sbjct: 393 VSWYDNEWGYSNRVLDLIEHM 413
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 68.5 bits (160), Expect = 3e-12
Identities = 36/85 (42%), Positives = 51/85 (60%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKL G+A RVP NVSVVDL V++ K E + ++AAE LKGIL ++ +VS D
Sbjct: 289 GKLNGIALRVPTPNVSVVDLVVQVSKKTFAEEVNAAFRDAAEKELKGILDVCDEPLVSVD 348
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F SS D++ + + D+ VK+
Sbjct: 349 FRCSDVSSTIDSSLTMVMGDDMVKV 373
Score = 37.5 bits (83), Expect = 0.006
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +3
Query: 243 LCEAISWYDNEYGYSSRVIDL 305
+ + I+WYDNE+GYS RV+DL
Sbjct: 370 MVKVIAWYDNEWGYSQRVVDL 390
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 66.9 bits (156), Expect = 9e-12
Identities = 34/85 (40%), Positives = 51/85 (60%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPASYEAIKQKVKEAAEGPLKGILGYTEDQVVSSD 180
GKL G+A RVP NVSVVDL V++ K E + +++AE LKGIL ++ +VS D
Sbjct: 286 GKLNGIALRVPTPNVSVVDLVVQVSKKTFAEEVNAAFRDSAEKELKGILDVCDEPLVSVD 345
Query: 181 FIGDSHSSIFDAAAGISLNDNFVKL 255
F S+ D++ + + D+ VK+
Sbjct: 346 FRCSDFSTTIDSSLTMVMGDDMVKV 370
Score = 37.5 bits (83), Expect = 0.006
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +3
Query: 243 LCEAISWYDNEYGYSSRVIDL 305
+ + I+WYDNE+GYS RV+DL
Sbjct: 367 MVKVIAWYDNEWGYSQRVVDL 387
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 64.9 bits (151), Expect = 4e-11
Identities = 34/86 (39%), Positives = 52/86 (60%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 GKLTGMAFRVPVANVSVVDLTVRLGKPA-SYEAIKQKVKEAAEGPLKGILGYTEDQVVSS 177
GKL G+A RVP NVSVVDL + + K + E + + ++AA GP+KGIL + +VS
Sbjct: 308 GKLNGIALRVPTPNVSVVDLVINVEKKGLTAEDVNEAFRKAANGPMKGILDVCDAPLVSV 367
Query: 178 DFIGDSHSSIFDAAAGISLNDNFVKL 255
DF S+ D++ + + D+ VK+
Sbjct: 368 DFRCSDVSTTIDSSLTMVMGDDMVKV 393
Score = 40.3 bits (90), Expect = 9e-04
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = +3
Query: 243 LCEAISWYDNEYGYSSRVIDLIKYIQSK 326
+ + ++WYDNE+GYS RV+DL + SK
Sbjct: 390 MVKVVAWYDNEWGYSQRVVDLAHLVASK 417
>At3g59510.1 68416.m06641 leucine-rich repeat family protein
contains leucine rich-repeat domains Pfam:PF00560,
INTERPRO:IPR001611; contains some similarity to Hcr2-5D
[Lycopersicon esculentum] gi|3894393|gb|AAC78596
Length = 419
Score = 27.9 bits (59), Expect = 5.0
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 459 YTACYLENTYRNGLLYSVFDEITKITYGSCK*N 557
+ + YLEN Y +G+L F +ITK G+ N
Sbjct: 357 WRSLYLENNYLSGILPEEFQKITKQIRGNLSNN 389
>At5g06420.2 68418.m00719 zinc finger (CCCH-type/C3HC4-type RING
finger) family protein contains Pfam domains PF00642:
Zinc finger C-x8-C-x5-C-x3-H type (and similar) and
PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 378
Score = 27.1 bits (57), Expect = 8.7
Identities = 24/68 (35%), Positives = 38/68 (55%), Gaps = 8/68 (11%)
Frame = +1
Query: 67 RLGKPASYEAIKQKVKEAAEGPL-KGILGYTE-------DQVVSSDFIGDSHSSIFDAAA 222
R+ K A + A+K K+A++ L KGI GYT+ +Q +SS+ G SH + A+A
Sbjct: 169 RVLKKADH-ALKGNKKKASDEKLYKGIHGYTDHKAGFRREQTISSEKAGGSHGPL-RASA 226
Query: 223 GISLNDNF 246
I ++ F
Sbjct: 227 HIRVSARF 234
>At5g06420.1 68418.m00718 zinc finger (CCCH-type/C3HC4-type RING
finger) family protein contains Pfam domains PF00642:
Zinc finger C-x8-C-x5-C-x3-H type (and similar) and
PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 378
Score = 27.1 bits (57), Expect = 8.7
Identities = 24/68 (35%), Positives = 38/68 (55%), Gaps = 8/68 (11%)
Frame = +1
Query: 67 RLGKPASYEAIKQKVKEAAEGPL-KGILGYTE-------DQVVSSDFIGDSHSSIFDAAA 222
R+ K A + A+K K+A++ L KGI GYT+ +Q +SS+ G SH + A+A
Sbjct: 169 RVLKKADH-ALKGNKKKASDEKLYKGIHGYTDHKAGFRREQTISSEKAGGSHGPL-RASA 226
Query: 223 GISLNDNF 246
I ++ F
Sbjct: 227 HIRVSARF 234
>At1g56450.1 68414.m06492 20S proteasome beta subunit G1 (PBG1)
(PRCH) identical to 20S proteasome beta subunit (PBG1)
GI:3421123 [Arabidopsis thaliana]; identical to cDNA
proteasome subunit prch GI:2511597
Length = 246
Score = 27.1 bits (57), Expect = 8.7
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +1
Query: 223 GISLNDNFVKLSVGTTMNMVIPAESLISSSTFNLKIKLLIKCMNVRIF 366
G+S D+ V G + I + + +F +KLL KCM V ++
Sbjct: 152 GVSFEDDHVATGFGNHLARPILRDEWHADLSFEDGVKLLEKCMRVLLY 199
>At1g24350.1 68414.m03071 expressed protein contains Pfam domain,
PF02681: Uncharacterized BCR, COG1963
Length = 168
Score = 27.1 bits (57), Expect = 8.7
Identities = 22/67 (32%), Positives = 33/67 (49%), Gaps = 8/67 (11%)
Frame = +1
Query: 19 AFRVPVANVSVVDLT-VRLGKPASYEAIKQKVKE-------AAEGPLKGILGYTEDQVVS 174
A + +A+V + D T VRL E + Q V E A PL+ +LG+T QVV+
Sbjct: 88 AIALILASVVMYDATGVRLHAGRQAEVLNQIVYELPAEHPLAESRPLRELLGHTPPQVVA 147
Query: 175 SDFIGDS 195
+G +
Sbjct: 148 GGMLGSA 154
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,000,900
Number of Sequences: 28952
Number of extensions: 174600
Number of successful extensions: 482
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 470
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 481
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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