BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30653 (713 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc... 98 1e-21 SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom... 98 1e-21 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 28 1.2 SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 26 6.1 SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 26 6.1 SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo... 25 8.1 >SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Schizosaccharomyces pombe|chr 1|||Manual Length = 176 Score = 98.3 bits (234), Expect = 1e-21 Identities = 60/146 (41%), Positives = 74/146 (50%), Gaps = 4/146 (2%) Frame = +3 Query: 201 FLSGSNCRQISFLVFPETLKKFKKTTGEIVXXXXXXXXXXXXXXNFGIWLRYESRSGVHN 380 F + + + F + + K KK TGEIV FGIW+RY+SRSG HN Sbjct: 29 FAPNESVAKSRYWYFLKMINKVKKATGEIVAINEISEPKPLKAKVFGIWIRYDSRSGTHN 88 Query: 381 MYREYRDLSLGGAVTQCYRDMGARHRARAHSIQIIK-VEVIKAARVAVHRSNSSTTAPFR 557 MY+E+RD + GAV Y DM ARHRAR SI+I+K VEV K V + + Sbjct: 89 MYKEFRDTTRVGAVEAMYADMAARHRARFRSIRILKVVEVEKKEDVRRNYVKQLLNPHLK 148 Query: 558 FPLPKR---VHHYKRLNTFAYKRPST 626 FPLP R V FA RPST Sbjct: 149 FPLPHRRTGVVGLAGKKVFAPHRPST 174 Score = 70.1 bits (164), Expect = 3e-13 Identities = 26/69 (37%), Positives = 48/69 (69%) Frame = +1 Query: 127 REYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWYFLRHLRSSRRPLVKLSLLRKF 306 +EY+V+GRK+P+E+EP P L++MR+F+P+ VAKSR+WYFL+ + ++ ++ + + Sbjct: 4 KEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINEI 63 Query: 307 QRKAPSRLR 333 P + + Sbjct: 64 SEPKPLKAK 72 >SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal protein L20a|Schizosaccharomyces pombe|chr 1|||Manual Length = 176 Score = 98.3 bits (234), Expect = 1e-21 Identities = 60/146 (41%), Positives = 74/146 (50%), Gaps = 4/146 (2%) Frame = +3 Query: 201 FLSGSNCRQISFLVFPETLKKFKKTTGEIVXXXXXXXXXXXXXXNFGIWLRYESRSGVHN 380 F + + + F + + K KK TGEIV FGIW+RY+SRSG HN Sbjct: 29 FAPNESVAKSRYWYFLKMINKVKKATGEIVAINEISEPKPLKAKVFGIWIRYDSRSGTHN 88 Query: 381 MYREYRDLSLGGAVTQCYRDMGARHRARAHSIQIIK-VEVIKAARVAVHRSNSSTTAPFR 557 MY+E+RD + GAV Y DM ARHRAR SI+I+K VEV K V + + Sbjct: 89 MYKEFRDTTRVGAVEAMYADMAARHRARFRSIRILKVVEVEKKEDVRRNYVKQLLNPHLK 148 Query: 558 FPLPKR---VHHYKRLNTFAYKRPST 626 FPLP R V FA RPST Sbjct: 149 FPLPHRRTGVVGLAGKKVFAPHRPST 174 Score = 70.1 bits (164), Expect = 3e-13 Identities = 26/69 (37%), Positives = 48/69 (69%) Frame = +1 Query: 127 REYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWYFLRHLRSSRRPLVKLSLLRKF 306 +EY+V+GRK+P+E+EP P L++MR+F+P+ VAKSR+WYFL+ + ++ ++ + + Sbjct: 4 KEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINEI 63 Query: 307 QRKAPSRLR 333 P + + Sbjct: 64 SEPKPLKAK 72 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = +2 Query: 491 GSNQGCACCRPQVKQFHNSTIQIPIAQTCAPLQ 589 G QGCA C P + F I+I A+T +Q Sbjct: 1071 GQPQGCALCYPYILAFEPDFIEIRNAETAELVQ 1103 >SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 25.8 bits (54), Expect = 6.1 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -1 Query: 287 NFTSGLLELLKCLRKYQKRD 228 N SG + L++C++KY RD Sbjct: 105 NNISGTINLIECMKKYNVRD 124 >SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1428 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +1 Query: 127 REYEVIGRKLPSENEPKPPLYKMRIFSPDPIVAKSRFWY 243 +E V+ + E P +R +SP+ ++ S FWY Sbjct: 470 QEILVLKSSIEKEKVEIVPFLDIRKYSPNEVLCMSPFWY 508 >SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 25.4 bits (53), Expect = 8.1 Identities = 10/32 (31%), Positives = 19/32 (59%) Frame = -3 Query: 234 TRFGDNWIRRENSHFIQGRLGLVLRRELAADN 139 ++F DN++ ++ R+G+ L REL D+ Sbjct: 3 SQFNDNFVATDDDVGTMARVGMALDRELNGDS 34 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,805,964 Number of Sequences: 5004 Number of extensions: 57537 Number of successful extensions: 154 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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