Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30650
(700 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB7B98 Cluster: PREDICTED: similar to asparagine... 137 2e-31
UniRef50_P08243 Cluster: Asparagine synthetase [glutamine-hydrol... 121 1e-26
UniRef50_UPI0000E46BAB Cluster: PREDICTED: hypothetical protein;... 110 3e-23
UniRef50_Q5UQE1 Cluster: Probable asparagine synthetase [glutami... 102 7e-21
UniRef50_Q8D1W9 Cluster: AsnB protein; n=2; Enterobacteriaceae|R... 99 9e-20
UniRef50_A5KBA9 Cluster: Asparagine synthetase [glutamine-hydrol... 99 9e-20
UniRef50_Q4WNT6 Cluster: Asparagine synthetase Asn2, putative; n... 99 9e-20
UniRef50_A2YBH4 Cluster: Putative uncharacterized protein; n=2; ... 97 5e-19
UniRef50_P49089 Cluster: Asparagine synthetase [glutamine-hydrol... 95 1e-18
UniRef50_P49078 Cluster: Asparagine synthetase [glutamine-hydrol... 94 3e-18
UniRef50_Q83S76 Cluster: Asparagine synthetase B; n=2; Enterobac... 94 3e-18
UniRef50_Q7KTW9 Cluster: CG33486-PA; n=6; Diptera|Rep: CG33486-P... 94 3e-18
UniRef50_A6R7L1 Cluster: Putative uncharacterized protein; n=1; ... 93 4e-18
UniRef50_A3J0G1 Cluster: Asparagine synthetase B; n=9; cellular ... 91 2e-17
UniRef50_Q4PHV2 Cluster: Putative uncharacterized protein; n=1; ... 91 3e-17
UniRef50_Q1VVM8 Cluster: Asparagine synthetase B; n=1; Psychrofl... 85 2e-15
UniRef50_Q9CIK5 Cluster: Asparagine synthetase B; n=8; Firmicute... 84 4e-15
UniRef50_Q4AC05 Cluster: 117M18_15; n=2; Brassica|Rep: 117M18_15... 83 8e-15
UniRef50_Q019J8 Cluster: Asparagine synthetase; n=2; Ostreococcu... 82 1e-14
UniRef50_A0CN48 Cluster: Chromosome undetermined scaffold_22, wh... 82 1e-14
UniRef50_Q4TFB6 Cluster: Chromosome undetermined SCAF4706, whole... 60 4e-13
UniRef50_Q01D26 Cluster: Asparagine synthetase; n=4; cellular or... 75 2e-12
UniRef50_O16924 Cluster: Asparaginyl trna synthetase protein 2; ... 73 5e-12
UniRef50_Q4C279 Cluster: Asparagine synthase, glutamine-hydrolyz... 66 6e-10
UniRef50_A0ZA07 Cluster: Asparagine synthase B; n=1; Nodularia s... 66 1e-09
UniRef50_A5UW35 Cluster: Asparagine synthase; n=2; Roseiflexus|R... 60 7e-08
UniRef50_A1I7L9 Cluster: Asparagine synthase B; n=1; Candidatus ... 55 2e-06
UniRef50_Q3CG33 Cluster: Asparagine synthase, glutamine-hydrolyz... 54 3e-06
UniRef50_Q2AIS3 Cluster: Glutamine amidotransferase, class-II:As... 51 2e-05
UniRef50_Q2CH15 Cluster: Asparagine synthetase B; n=1; Oceanicol... 49 1e-04
UniRef50_Q4ZNC7 Cluster: Asparagine synthase, glutamine-hydrolyz... 46 7e-04
UniRef50_UPI000038414B Cluster: COG0367: Asparagine synthase (gl... 44 0.003
UniRef50_Q1FIF7 Cluster: Asparagine synthase, glutamine-hydrolyz... 44 0.003
UniRef50_A0RRB3 Cluster: Asparagine synthase; n=1; Campylobacter... 44 0.005
UniRef50_A3CX83 Cluster: Asparagine synthase; n=1; Methanoculleu... 44 0.005
UniRef50_Q67SU9 Cluster: Asparagine synthase B; n=1; Symbiobacte... 43 0.006
UniRef50_Q7MBG3 Cluster: PUTATIVE ASPARAGINE SYNTHETASE (GLUTAMI... 42 0.015
UniRef50_Q3EU47 Cluster: Asparagine synthetase; n=1; Bacillus th... 41 0.025
UniRef50_O26514 Cluster: Asparagine synthetase; n=1; Methanother... 41 0.025
UniRef50_Q2IZV9 Cluster: Asparagine synthase, glutamine-hydrolyz... 40 0.044
UniRef50_Q1VN43 Cluster: Asparagine synthase family protein; n=1... 40 0.059
UniRef50_Q8TPE7 Cluster: Asparagine synthase; n=3; Methanosarcin... 40 0.059
UniRef50_Q12VP2 Cluster: Asparagine synthase, glutamine-hydrolyz... 40 0.059
UniRef50_A5UZF1 Cluster: Asparagine synthase; n=9; cellular orga... 40 0.078
UniRef50_Q60BD5 Cluster: Asparagine synthetase, glutamine-hydrol... 39 0.14
UniRef50_A5V1J9 Cluster: Asparagine synthase; n=5; Chloroflexace... 39 0.14
UniRef50_UPI00015BCA27 Cluster: UPI00015BCA27 related cluster; n... 38 0.18
UniRef50_Q982D6 Cluster: Mlr9125 protein; n=1; Mesorhizobium lot... 38 0.18
UniRef50_Q30U64 Cluster: Asparagine synthase, glutamine-hydrolyz... 38 0.18
UniRef50_A3I8W6 Cluster: Asparagine synthetase; n=4; Bacteria|Re... 38 0.18
UniRef50_A7IB45 Cluster: Asparagine synthase; n=1; Candidatus Me... 38 0.18
UniRef50_Q58516 Cluster: Putative asparagine synthetase [glutami... 38 0.18
UniRef50_Q7M7N7 Cluster: WBFR PROTEIN; n=1; Wolinella succinogen... 38 0.24
UniRef50_Q3AVQ8 Cluster: Asparagine synthase, glutamine-hydrolyz... 38 0.24
UniRef50_Q30U87 Cluster: Asparagine synthase, glutamine-hydrolyz... 38 0.24
UniRef50_A0YEF5 Cluster: Asparagine synthase; n=1; marine gamma ... 38 0.24
UniRef50_P54420 Cluster: Asparagine synthetase [glutamine-hydrol... 38 0.24
UniRef50_Q2SJG5 Cluster: Asparagine synthase; n=1; Hahella cheju... 38 0.31
UniRef50_Q1QD48 Cluster: Asparagine synthase, glutamine-hydrolyz... 38 0.31
UniRef50_Q1NS49 Cluster: Asparagine synthase; n=1; delta proteob... 38 0.31
UniRef50_Q01Z46 Cluster: Asparagine synthase; n=1; Solibacter us... 38 0.31
UniRef50_A7H9Q9 Cluster: Asparagine synthase; n=3; Proteobacteri... 38 0.31
UniRef50_O05272 Cluster: Asparagine synthetase [glutamine-hydrol... 38 0.31
UniRef50_A4B944 Cluster: Asparagine synthase; n=1; Reinekea sp. ... 37 0.41
UniRef50_Q0W279 Cluster: Putative asparagine synthetase, glutami... 37 0.41
UniRef50_Q2LWL8 Cluster: Asparagine synthetase; n=1; Syntrophus ... 37 0.55
UniRef50_Q8TZ36 Cluster: Asparagine synthase; n=1; Methanopyrus ... 37 0.55
UniRef50_Q62A30 Cluster: Asparagine synthase; n=24; Bacteria|Rep... 36 0.72
UniRef50_Q0LLW6 Cluster: Asparagine synthase, glutamine-hydrolyz... 36 0.72
UniRef50_A6Q4Y9 Cluster: Asparagine synthase, glutamine-hydrolyz... 36 0.72
UniRef50_A6DEM6 Cluster: Probable asparagine synthase, glutamine... 36 0.72
UniRef50_Q2SJH5 Cluster: Asparagine synthase; n=1; Hahella cheju... 36 0.96
UniRef50_Q08YT4 Cluster: Asparagine synthase, glutamine-hydrolyz... 36 0.96
UniRef50_A0A004 Cluster: MoeF5; n=1; Streptomyces ghanaensis|Rep... 36 0.96
UniRef50_Q4FTY4 Cluster: Probable asparagine synthase, glutamine... 36 1.3
UniRef50_Q319R2 Cluster: Asparagine synthase, glutamine-hydrolyz... 36 1.3
UniRef50_A7DMN1 Cluster: Asparagine synthase; n=1; Candidatus Ni... 36 1.3
UniRef50_A0RUC0 Cluster: Asparagine synthase; n=1; Cenarchaeum s... 36 1.3
UniRef50_Q216T1 Cluster: Asparagine synthase, glutamine-hydrolyz... 35 1.7
UniRef50_A6Q5J5 Cluster: Putative uncharacterized protein; n=1; ... 35 1.7
UniRef50_A4TWX4 Cluster: Asparagine synthase, glutamine-hydrolyz... 35 1.7
UniRef50_A4BPQ6 Cluster: Asparagine synthase; n=1; Nitrococcus m... 35 1.7
UniRef50_A1ASZ8 Cluster: Asparagine synthase; n=2; Desulfuromona... 35 1.7
UniRef50_Q31I32 Cluster: Asparagine synthase, glutamine-hydrolyz... 35 2.2
UniRef50_Q12IB8 Cluster: Asparagine synthase, glutamine-hydrolyz... 35 2.2
UniRef50_A6PQS0 Cluster: Asparagine synthase; n=1; Victivallis v... 34 2.9
UniRef50_A3ZYU5 Cluster: Asparagine synthetase [glutamine-hydrol... 34 2.9
UniRef50_Q9UZJ0 Cluster: Putative putative asparagine synthetase... 34 2.9
UniRef50_Q9HL64 Cluster: Hypothetical membrane protein; n=1; The... 34 2.9
UniRef50_Q1N9Z8 Cluster: Putative uncharacterized protein; n=1; ... 34 3.9
UniRef50_Q02LN1 Cluster: Asparagine synthetase, glutamine-hydrol... 34 3.9
UniRef50_Q02CD2 Cluster: Asparagine synthase; n=1; Solibacter us... 34 3.9
UniRef50_Q01UW3 Cluster: Asparagine synthase; n=1; Solibacter us... 34 3.9
UniRef50_A6DB45 Cluster: Putative uncharacterized protein; n=1; ... 34 3.9
UniRef50_A2EQ39 Cluster: Asparagine synthase family protein; n=1... 34 3.9
UniRef50_Q6W388 Cluster: Asparagine synthase; n=1; uncultured cr... 34 3.9
UniRef50_P34303 Cluster: Putative zinc finger protein C06E1.8; n... 34 3.9
UniRef50_Q0IDQ7 Cluster: Asparagine synthase; n=1; Synechococcus... 33 5.1
UniRef50_A5ZSA8 Cluster: Putative uncharacterized protein; n=1; ... 33 5.1
UniRef50_O28833 Cluster: Asparagine synthetase; n=1; Archaeoglob... 33 5.1
UniRef50_UPI0000384B0F Cluster: COG0367: Asparagine synthase (gl... 33 6.7
UniRef50_Q5FML5 Cluster: Asn synthetase; n=20; Bacteria|Rep: Asn... 33 6.7
UniRef50_Q11P23 Cluster: Asparagine synthase, glutamine-hydrolyz... 33 6.7
UniRef50_A4X6Q9 Cluster: Asparagine synthase; n=1; Salinispora t... 33 6.7
UniRef50_A0M2W8 Cluster: Asparagine synthetase [glutamine-hydrol... 33 6.7
UniRef50_A0LYW1 Cluster: Asparagine synthetase [glutamine-hydrol... 33 6.7
UniRef50_A5UJI7 Cluster: Asparagine synthetase, AsnB; n=1; Metha... 33 6.7
UniRef50_Q5FPJ6 Cluster: Asparagine synthetase [glutamine-hydrol... 33 8.9
UniRef50_Q2ILL8 Cluster: Asparagine synthase, glutamine-hydrolyz... 33 8.9
UniRef50_Q6QW19 Cluster: Asparagine synthase-like protein; n=1; ... 33 8.9
UniRef50_Q220Y1 Cluster: Asparagine synthase, glutamine-hydrolyz... 33 8.9
UniRef50_A6GFB8 Cluster: Asparagine synthase, glutamine-hydrolyz... 33 8.9
UniRef50_A3T3D5 Cluster: Asparagine synthase; n=1; Sulfitobacter... 33 8.9
UniRef50_A0Q7S4 Cluster: Glutamine amidotransferase/asparagine s... 33 8.9
UniRef50_Q58456 Cluster: Putative asparagine synthetase [glutami... 33 8.9
>UniRef50_UPI0000DB7B98 Cluster: PREDICTED: similar to asparagine
synthetase; n=1; Apis mellifera|Rep: PREDICTED: similar
to asparagine synthetase - Apis mellifera
Length = 528
Score = 137 bits (332), Expect = 2e-31
Identities = 64/83 (77%), Positives = 73/83 (87%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
+IY LE+ DITTIRAS+ MYL+S+YIK+ T TTV+FSGEG DELAQGYIYFRDAP+ +A
Sbjct: 324 LIYQLETCDITTIRASIGMYLVSRYIKQNTATTVIFSGEGADELAQGYIYFRDAPNPIEA 383
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H ESVRLL DIYLYDGLRADRTT
Sbjct: 384 HNESVRLLKDIYLYDGLRADRTT 406
Score = 89.0 bits (211), Expect = 1e-16
Identities = 45/86 (52%), Positives = 57/86 (66%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A +KRLMSDR I I A++ AK+ L YKI++FAIGMGDSPD+ AAR
Sbjct: 239 AVQKRLMSDREIGCLLSGGLDSSLIAALLVKHAKDANLSYKIKSFAIGMGDSPDIIAARQ 298
Query: 184 VADYLGTEHHEVQFDENDIRKDLETL 261
VA+Y+GTEHHE+ F END+ L+ L
Sbjct: 299 VAEYIGTEHHEIIFFENDVIDILDKL 324
Score = 62.9 bits (146), Expect = 7e-09
Identities = 30/43 (69%), Positives = 37/43 (86%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSF 637
AFSLELRVPFLDIQFT++YLS+ RQPQ GR+ K+LLRS++
Sbjct: 408 AFSLELRVPFLDIQFTNYYLSLDAASRQPQ-GRIEKYLLRSAY 449
>UniRef50_P08243 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=35; Eumetazoa|Rep: Asparagine
synthetase [glutamine-hydrolyzing] - Homo sapiens
(Human)
Length = 561
Score = 121 bits (292), Expect = 1e-26
Identities = 55/83 (66%), Positives = 70/83 (84%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VI+ LE+YDITT+RAS+ MYL+SKYI++ TD+ V+FSGEG+DEL QGYIYF APS + A
Sbjct: 327 VIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKA 386
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
+ES RLL ++YL+D LRADRTT
Sbjct: 387 EEESERLLRELYLFDVLRADRTT 409
Score = 76.2 bits (179), Expect = 7e-13
Identities = 41/90 (45%), Positives = 57/90 (63%), Gaps = 1/90 (1%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A +KRLM+DRRI + A + KE ++ Y +QTFAIGM DSPDL AAR
Sbjct: 242 AVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARK 301
Query: 184 VADYLGTEHHEVQFD-ENDIRKDLETLFTI 270
VAD++G+EH+EV F+ E I+ E +F++
Sbjct: 302 VADHIGSEHYEVLFNSEEGIQALDEVIFSL 331
Score = 72.5 bits (170), Expect = 9e-12
Identities = 33/58 (56%), Positives = 44/58 (75%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A LELRVPFLD +F+ +YLS+PP++R P+NG + KHLLR +F S L+P +LWR K
Sbjct: 411 AHGLELRVPFLDHRFSSYYLSLPPEMRIPKNG-IEKHLLRETFEDSNLIPKEILWRPK 467
>UniRef50_UPI0000E46BAB Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 433
Score = 110 bits (265), Expect = 3e-23
Identities = 53/84 (63%), Positives = 65/84 (77%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VI LESYDITT+RAS+ MYL+++YI+++TDT VV+SGEG D LAQGYIYF P
Sbjct: 231 HVIKSLESYDITTLRASVGMYLVAQYIRKETDTIVVYSGEGADCLAQGYIYFHKQPDASA 290
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
ES +LL D+YLYD LRADRTT
Sbjct: 291 GDDESRKLLKDLYLYDVLRADRTT 314
Score = 63.7 bits (148), Expect = 4e-09
Identities = 31/74 (41%), Positives = 44/74 (59%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A RKRLM +RRI ++++V + Y +QTF+IGM SPD+ AAR
Sbjct: 147 AVRKRLMGERRIGCMLSGGLDSSLVSSLVMKAMVKDGFDYPLQTFSIGMKGSPDVVAARK 206
Query: 184 VADYLGTEHHEVQF 225
VA ++G+EHHE+ F
Sbjct: 207 VASHIGSEHHEIGF 220
>UniRef50_Q5UQE1 Cluster: Probable asparagine synthetase
[glutamine-hydrolyzing]; n=1; Acanthamoeba polyphaga
mimivirus|Rep: Probable asparagine synthetase
[glutamine-hydrolyzing] - Mimivirus
Length = 550
Score = 102 bits (245), Expect = 7e-21
Identities = 44/83 (53%), Positives = 63/83 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VI +E+YDITTIRAS P ++++KYI+E TD V+ SGEG+DE+ Y Y R AP+ ++
Sbjct: 323 DVIKTIETYDITTIRASTPQFIMAKYIQENTDIRVLLSGEGSDEIHGSYKYMRSAPNSQE 382
Query: 435 AHKESVRLLSDIYLYDGLRADRT 503
HKE++RLL ++YL+D R DRT
Sbjct: 383 FHKETIRLLEELYLFDNKRTDRT 405
Score = 45.6 bits (103), Expect = 0.001
Identities = 35/93 (37%), Positives = 44/93 (47%), Gaps = 8/93 (8%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A RKRL +DR+I I AI L P I F++G SPD+AAAR
Sbjct: 242 AVRKRLYADRQIGFLLSGGLDSSLIVAIATRLLG----PTNIVCFSVGFEGSPDVAAARE 297
Query: 184 VADYLGTE-HHEVQFDE-------NDIRKDLET 258
V +LG + HH V F ND+ K +ET
Sbjct: 298 VVKFLGIKNHHIVPFSVDIGLNAINDVIKTIET 330
Score = 41.9 bits (94), Expect = 0.015
Identities = 20/55 (36%), Positives = 33/55 (60%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
LE+RVPFLD + ++I P L ++ + K ++R +F G LP+ +L+R K
Sbjct: 411 LEVRVPFLDFNYVDFIMNIDPNLLMYKSDYIEKKIIRDAF--KGYLPENILYRPK 463
>UniRef50_Q8D1W9 Cluster: AsnB protein; n=2; Enterobacteriaceae|Rep:
AsnB protein - Wigglesworthia glossinidia brevipalpis
Length = 545
Score = 99.1 bits (236), Expect = 9e-20
Identities = 45/82 (54%), Positives = 63/82 (76%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIY++E+YDITTIRAS+PMYL+S+ IK + ++ SGEG DE+ GY+YF +APSEKD
Sbjct: 304 VIYYVETYDITTIRASIPMYLISQKIKS-SGIKMILSGEGADEIFGGYLYFHNAPSEKDL 362
Query: 438 HKESVRLLSDIYLYDGLRADRT 503
H E VR L +++YD RA+++
Sbjct: 363 HDELVRKLKSLHIYDCARANKS 384
Score = 46.0 bits (104), Expect = 9e-04
Identities = 25/54 (46%), Positives = 37/54 (68%), Gaps = 3/54 (5%)
Frame = +1
Query: 85 IVNYLAKE--YK-LPYKIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
I + +KE YK L K+++F+IG+ +SPDL ++R A YL T+H EV+F E D
Sbjct: 243 IASIASKELNYKNLNKKLKSFSIGLENSPDLHSSRICARYLNTDHCEVKFTEQD 296
>UniRef50_A5KBA9 Cluster: Asparagine synthetase
[glutamine-hydrolyzing], putative; n=10; Plasmodium|Rep:
Asparagine synthetase [glutamine-hydrolyzing], putative
- Plasmodium vivax
Length = 613
Score = 99.1 bits (236), Expect = 9e-20
Identities = 45/84 (53%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YDITTIRAS PMY+LS+ IK +V SGEG DE+ GY+YF AP+ ++
Sbjct: 347 DVIYHIETYDITTIRASTPMYILSRLIKSSC-VKMVLSGEGADEIFGGYLYFHKAPNREE 405
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E R + D++LYD LRA+++T
Sbjct: 406 FHRELQRKVHDLHLYDCLRANKST 429
Score = 50.8 bits (116), Expect = 3e-05
Identities = 25/58 (43%), Positives = 35/58 (60%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
AF +E RVPFLD+ ++I PK + G + K +LR +FA G LPD +L+R K
Sbjct: 431 AFGIEARVPFLDLHLLDVVMNIDPKEKMCSEGHIEKDILRRAFA--GYLPDHILYRQK 486
Score = 40.3 bits (90), Expect = 0.044
Identities = 16/33 (48%), Positives = 26/33 (78%)
Frame = +1
Query: 127 IQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
+++F+IG+ +SPDL AA+ VA++LG+ H E F
Sbjct: 304 LKSFSIGLRNSPDLKAAKEVANFLGSHHTEFHF 336
>UniRef50_Q4WNT6 Cluster: Asparagine synthetase Asn2, putative;
n=22; Dikarya|Rep: Asparagine synthetase Asn2, putative
- Aspergillus fumigatus (Sartorya fumigata)
Length = 639
Score = 99.1 bits (236), Expect = 9e-20
Identities = 46/84 (54%), Positives = 66/84 (78%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYHLE+YD+TTIRAS PMYLLS+ IK +V SGEG+DE+ GY+YF AP++++
Sbjct: 379 DVIYHLETYDVTTIRASTPMYLLSRKIKAM-GVKMVLSGEGSDEIFGGYLYFHAAPNKEE 437
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
HKE+VR + +++L D LRA+++T
Sbjct: 438 FHKETVRRVKNLHLADCLRANKST 461
Score = 43.6 bits (98), Expect = 0.005
Identities = 23/64 (35%), Positives = 35/64 (54%), Gaps = 6/64 (9%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSG------LLPDCVL 670
A+ LE RVPFLD +F + + P+ + R+ K++LR +F S LP+ +L
Sbjct: 463 AWGLEARVPFLDKEFLEAAMGVDPQEKMITKERIEKYILRKAFDTSDEPDTKPYLPEKIL 522
Query: 671 WRHK 682
WR K
Sbjct: 523 WRQK 526
Score = 36.7 bits (81), Expect = 0.55
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
++ +F+IG+ +PD AA VA +LGT+HH F
Sbjct: 335 QLHSFSIGLPGAPDTEAALEVARFLGTKHHAFTF 368
>UniRef50_A2YBH4 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 530
Score = 96.7 bits (230), Expect = 5e-19
Identities = 43/83 (51%), Positives = 64/83 (77%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+
Sbjct: 281 VIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEIFGGYLYFHKAPNKKEF 339
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++LYD LRA+++T
Sbjct: 340 HEETCRKIKALHLYDCLRANKST 362
Score = 52.8 bits (121), Expect = 8e-06
Identities = 30/75 (40%), Positives = 40/75 (53%), Gaps = 4/75 (5%)
Frame = +1
Query: 13 KRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPY----KIQTFAIGMGDSPDLAAAR 180
KRLM+D + ++V+ E K+ K+ TF IG+ SPDL AA+
Sbjct: 195 KRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNKLHTFCIGLKGSPDLRAAK 254
Query: 181 TVADYLGTEHHEVQF 225
VADYLGT HHE+ F
Sbjct: 255 EVADYLGTVHHELHF 269
Score = 37.5 bits (83), Expect = 0.31
Identities = 23/62 (37%), Positives = 36/62 (58%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A+ +E RVPFLD F + + I P K+ + GR+ K +LR++F + LP +L+R
Sbjct: 364 AWGVEARVPFLDKNFINVAMDIDPEWKMIKRDLGRIEKWVLRNAFDDEEKPYLPKHILYR 423
Query: 677 HK 682
K
Sbjct: 424 QK 425
>UniRef50_P49089 Cluster: Asparagine synthetase
[glutamine-hydrolyzing] 1; n=139; cellular
organisms|Rep: Asparagine synthetase
[glutamine-hydrolyzing] 1 - Saccharomyces cerevisiae
(Baker's yeast)
Length = 572
Score = 95.5 bits (227), Expect = 1e-18
Identities = 45/84 (53%), Positives = 64/84 (76%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYHLE+YD+TTIRAS PM+LLS+ IK + +V SGEG+DE+ GY+YF APS +
Sbjct: 330 DVIYHLETYDVTTIRASTPMFLLSRKIKAQ-GVKMVLSGEGSDEIFGGYLYFAQAPSAAE 388
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H ESV+ + +++L D LRA+++T
Sbjct: 389 FHTESVQRVKNLHLADCLRANKST 412
Score = 50.0 bits (114), Expect = 5e-05
Identities = 27/66 (40%), Positives = 41/66 (62%), Gaps = 8/66 (12%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSFAKSG------LLPDC 664
A+ LE RVPFLD +F ++I P K+ +P+ GR+ K++LR +F +G LP+
Sbjct: 414 AWGLEARVPFLDREFLQLCMNIDPNEKMIKPKEGRIEKYILRKAFDTTGEPDAKPYLPEE 473
Query: 665 VLWRHK 682
+LWR K
Sbjct: 474 ILWRQK 479
Score = 41.1 bits (92), Expect = 0.025
Identities = 17/33 (51%), Positives = 25/33 (75%)
Frame = +1
Query: 127 IQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
+ +FAIG+ ++PDL AAR VA ++G+ HHE F
Sbjct: 287 LHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTF 319
>UniRef50_P49078 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=68; cellular organisms|Rep:
Asparagine synthetase [glutamine-hydrolyzing] -
Arabidopsis thaliana (Mouse-ear cress)
Length = 584
Score = 94.3 bits (224), Expect = 3e-18
Identities = 42/84 (50%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG DE+ GY+YF AP++K+
Sbjct: 305 DVIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKE 363
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ YD LRA+++T
Sbjct: 364 FHQETCRKIKALHKYDCLRANKST 387
Score = 46.4 bits (105), Expect = 7e-04
Identities = 26/62 (41%), Positives = 38/62 (61%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
AF LE RVPFLD F + +S+ P K+ +P+ GR+ K +LR +F + LP +L+R
Sbjct: 389 AFGLEARVPFLDKDFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
Score = 45.6 bits (103), Expect = 0.001
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ +F +G+ SPDL A + VA+YLGT HHE F D
Sbjct: 261 QLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 298
>UniRef50_Q83S76 Cluster: Asparagine synthetase B; n=2;
Enterobacteriaceae|Rep: Asparagine synthetase B -
Shigella flexneri
Length = 515
Score = 93.9 bits (223), Expect = 3e-18
Identities = 43/82 (52%), Positives = 62/82 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YD+TTIRAS PMYL+S+ IK +V SGEG+DE+ GY+YF AP+ K+
Sbjct: 272 DVIYHIETYDVTTIRASTPMYLMSRKIKAM-GIKMVLSGEGSDEVFGGYLYFHKAPNVKE 330
Query: 435 AHKESVRLLSDIYLYDGLRADR 500
H+E+VR L +++YD RA++
Sbjct: 331 LHEETVRKLLALHMYDCARANK 352
Score = 46.8 bits (106), Expect = 5e-04
Identities = 24/58 (41%), Positives = 33/58 (56%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A+ +E RVPFLD +F + I P+ + NG++ KH+LR F LP V WR K
Sbjct: 356 AWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECF--ESYLPASVAWRQK 411
>UniRef50_Q7KTW9 Cluster: CG33486-PA; n=6; Diptera|Rep: CG33486-PA -
Drosophila melanogaster (Fruit fly)
Length = 558
Score = 93.9 bits (223), Expect = 3e-18
Identities = 42/82 (51%), Positives = 60/82 (73%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
++IYHLE+YD+TT+R SLPM LL++YIK T ++ SGEG DE+ GY+YF APS D
Sbjct: 316 DIIYHLETYDVTTVRCSLPMLLLARYIKS-TGIKMILSGEGADEIFGGYLYFHKAPSYND 374
Query: 435 AHKESVRLLSDIYLYDGLRADR 500
H+E V+ + ++L D LRA++
Sbjct: 375 FHEELVKRVRQLHLSDCLRANK 396
Score = 49.2 bits (112), Expect = 1e-04
Identities = 30/67 (44%), Positives = 38/67 (56%), Gaps = 9/67 (13%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP---------KLRQPQNGRLRKHLLRSSFAKSGLLPD 661
A +ELRVPFLD F +H + I P K + Q RL K++LR++FA LPD
Sbjct: 400 AKGVELRVPFLDTGFVNHVMQIRPEDKIPGPLNKFGEVQQKRLEKYVLRAAFA-DNYLPD 458
Query: 662 CVLWRHK 682
VLWR K
Sbjct: 459 EVLWRQK 465
Score = 48.8 bits (111), Expect = 1e-04
Identities = 22/75 (29%), Positives = 41/75 (54%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A R L D ++ I +I + +E ++++TF++G+ D+PD AAR+
Sbjct: 232 AVRSHLQCDVQMGALLSGGVDSSLIASIATKIMRERDPNFRLKTFSVGLRDAPDFQAARS 291
Query: 184 VADYLGTEHHEVQFD 228
VA Y+ ++H E+ F+
Sbjct: 292 VAKYIDSDHKEIIFE 306
>UniRef50_A6R7L1 Cluster: Putative uncharacterized protein; n=1;
Ajellomyces capsulatus NAm1|Rep: Putative
uncharacterized protein - Ajellomyces capsulatus NAm1
Length = 539
Score = 93.5 bits (222), Expect = 4e-18
Identities = 45/84 (53%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYHLE+YD+TTIRAS PMYLLS+ IK +V SGEG+DE+ GY+YF APS++
Sbjct: 353 DVIYHLETYDVTTIRASTPMYLLSRKIKG-LGVKMVLSGEGSDEIFGGYLYFHAAPSKEA 411
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H E+V + +++L D LRA+++T
Sbjct: 412 FHDETVSRVKNLHLADCLRANKST 435
Score = 40.3 bits (90), Expect = 0.044
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = +1
Query: 127 IQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
+ +F+IG+ D+PD AA VA +LGT+HH F D
Sbjct: 310 LHSFSIGLPDAPDTKAALEVAKFLGTKHHAFTFTLED 346
>UniRef50_A3J0G1 Cluster: Asparagine synthetase B; n=9; cellular
organisms|Rep: Asparagine synthetase B - Flavobacteria
bacterium BAL38
Length = 538
Score = 91.1 bits (216), Expect = 2e-17
Identities = 43/83 (51%), Positives = 60/83 (72%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
+I+HLE+YD+T+IRAS PMY LSK I +K +V SGEG DE+ GY+YFR+APS D
Sbjct: 295 LIWHLETYDVTSIRASTPMYFLSKAITDK-GIKMVLSGEGADEIFGGYLYFRNAPSVLDF 353
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
KE++ + ++ D LRAD++T
Sbjct: 354 QKETIERVQKLFTADLLRADKST 376
Score = 44.8 bits (101), Expect = 0.002
Identities = 27/75 (36%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDS-PDLAAAR 180
A KRLM D I +I + L + ++ +F+IG+ S PDL AA+
Sbjct: 211 AVEKRLMCDVPFGVLLSGGLDSSLIASITSRLLEG--TGQELHSFSIGLDASAPDLVAAK 268
Query: 181 TVADYLGTEHHEVQF 225
VAD++GT HHE+ F
Sbjct: 269 KVADFIGTTHHEIHF 283
Score = 42.3 bits (95), Expect = 0.011
Identities = 25/62 (40%), Positives = 36/62 (58%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQ--NGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A LE RVPFLD F + I P+ + P+ NG + K++LR +F + LP+ +LWR
Sbjct: 378 AHGLEARVPFLDKAFLDLAIKIQPEEKMPKTYNG-IEKYILRKAFDTPEKPFLPEEILWR 436
Query: 677 HK 682
K
Sbjct: 437 QK 438
>UniRef50_Q4PHV2 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 634
Score = 90.6 bits (215), Expect = 3e-17
Identities = 42/84 (50%), Positives = 61/84 (72%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VI+HLE+YD+TT+RAS PMYLLS+ IK +V SGEG+DE+ GY+YF AP +
Sbjct: 365 DVIFHLETYDVTTVRASTPMYLLSRKIKAM-GVKMVLSGEGSDEIFGGYLYFHAAPDKTS 423
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E VR + +++ D LRA+++T
Sbjct: 424 FHQECVRRVKNLHTADCLRANKST 447
Score = 43.6 bits (98), Expect = 0.005
Identities = 18/34 (52%), Positives = 24/34 (70%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
++ +F+IG+ SPDL AAR A +LGT HHE F
Sbjct: 321 RLHSFSIGLPGSPDLIAARKAAQFLGTVHHEYTF 354
>UniRef50_Q1VVM8 Cluster: Asparagine synthetase B; n=1;
Psychroflexus torquis ATCC 700755|Rep: Asparagine
synthetase B - Psychroflexus torquis ATCC 700755
Length = 524
Score = 84.6 bits (200), Expect = 2e-15
Identities = 39/83 (46%), Positives = 57/83 (68%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+ +Y L SYD+TTIRAS+ L++KY+++ +D V+FSGE DE Y+YF++AP +
Sbjct: 292 HTVYMLGSYDVTTIRASVGHQLVAKYVRDHSDVKVLFSGETADEFG-SYLYFQNAPDSES 350
Query: 435 AHKESVRLLSDIYLYDGLRADRT 503
ES+RLL DI +D RADR+
Sbjct: 351 FQHESIRLLKDIQFFDMKRADRS 373
Score = 56.8 bits (131), Expect = 5e-07
Identities = 24/55 (43%), Positives = 36/55 (65%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
LE RVPF D F ++SIPP+ R + ++ K+L+R +F K L+P+ +LWR K
Sbjct: 379 LEARVPFADKAFVKFFMSIPPESRLFSDQKIEKYLIRKAFEKENLIPEEILWRRK 433
Score = 51.6 bits (118), Expect = 2e-05
Identities = 29/82 (35%), Positives = 46/82 (56%), Gaps = 1/82 (1%)
Frame = +1
Query: 13 KRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYK-IQTFAIGMGDSPDLAAARTVA 189
KR+MS+R + + I LA++ K K ++TF+IG+ SPD+ AA+ VA
Sbjct: 213 KRMMSERPLGCLLSGGLDSSLVAGI---LARKLKEKGKQLETFSIGLEGSPDIVAAKIVA 269
Query: 190 DYLGTEHHEVQFDENDIRKDLE 255
D++G+ HH + E D +LE
Sbjct: 270 DHIGSIHHSIVCTEQDFLDELE 291
>UniRef50_Q9CIK5 Cluster: Asparagine synthetase B; n=8;
Firmicutes|Rep: Asparagine synthetase B - Lactococcus
lactis subsp. lactis (Streptococcus lactis)
Length = 530
Score = 83.8 bits (198), Expect = 4e-15
Identities = 42/81 (51%), Positives = 58/81 (71%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VI+HLE++DITTIRAS+ MYL+ KYI E T+ V+ +GE +DE+ GY Y APS
Sbjct: 297 VIWHLETWDITTIRASIGMYLICKYIHENTELKVLMTGEVSDEMF-GYKYTDFAPSAGAF 355
Query: 438 HKESVRLLSDIYLYDGLRADR 500
KE+ + + ++Y+YD LRADR
Sbjct: 356 QKEAQKRVHELYMYDVLRADR 376
Score = 48.4 bits (110), Expect = 2e-04
Identities = 26/60 (43%), Positives = 37/60 (61%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHKGS 688
A SLE RVPF D+ F + +SI P + G+ K+LLR +F + LLPD +L+R K +
Sbjct: 380 AHSLEARVPFADLDFAAYVMSINPSRKLNTYGK-GKYLLRHAFEGTELLPDEILFREKAA 438
Score = 44.8 bits (101), Expect = 0.002
Identities = 31/80 (38%), Positives = 42/80 (52%), Gaps = 1/80 (1%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSP-DLAAAR 180
A +KRL +D + + AI AKE + P I+TFAIGM P DL AR
Sbjct: 216 AVKKRLHADAPMGYLLSGGLDSSLVCAIA---AKELESP--IKTFAIGMETDPIDLKYAR 270
Query: 181 TVADYLGTEHHEVQFDENDI 240
VAD+L T+H E+ + D+
Sbjct: 271 EVADFLRTDHTEILMTKEDV 290
>UniRef50_Q4AC05 Cluster: 117M18_15; n=2; Brassica|Rep: 117M18_15 -
Brassica campestris (Field mustard)
Length = 608
Score = 82.6 bits (195), Expect = 8e-15
Identities = 37/75 (49%), Positives = 54/75 (72%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+
Sbjct: 307 VIYHVETYDVTTIRASTPMFLMSRKIK-SLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEL 365
Query: 438 HKESVRLLSDIYLYD 482
H+E+ R + + D
Sbjct: 366 HEETCRKATPLEFLD 380
Score = 50.8 bits (116), Expect = 3e-05
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
K+ TF IG+ SPDL A + VADYLGT HHE+ F
Sbjct: 262 KLHTFCIGLKGSPDLKAGKEVADYLGTRHHELHF 295
Score = 41.9 bits (94), Expect = 0.015
Identities = 25/62 (40%), Positives = 37/62 (59%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A+ +E RVPFLD F + I P K+ +P GR+ K +LR++F K+ LP +L+R
Sbjct: 418 AWGVEARVPFLDKAFLDVAMGIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYR 477
Query: 677 HK 682
K
Sbjct: 478 QK 479
>UniRef50_Q019J8 Cluster: Asparagine synthetase; n=2;
Ostreococcus|Rep: Asparagine synthetase - Ostreococcus
tauri
Length = 554
Score = 82.2 bits (194), Expect = 1e-14
Identities = 40/84 (47%), Positives = 54/84 (64%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
NVIYHLE+Y+ IR+++P Y L+K + VV +GEG+DEL GY+YFRDAPS K
Sbjct: 312 NVIYHLETYEPELIRSAIPNYFLAKLTSQHVK--VVLTGEGSDELFAGYLYFRDAPSSKH 369
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H E R+ ++ + RADR T
Sbjct: 370 VHNELRRIFGHLHNVNCQRADRMT 393
Score = 43.6 bits (98), Expect = 0.005
Identities = 20/63 (31%), Positives = 38/63 (60%), Gaps = 2/63 (3%)
Frame = +1
Query: 79 TAIVNYLAKEY--KLPYKIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDENDIRKDL 252
+A+V+ + K + K + +F +G SPD+ AAR ++++LGT+HHE F + ++
Sbjct: 251 SAVVSTVLKPFLEKSGQEYLSFTVGQEGSPDVTAARMMSEFLGTKHHEYLFTSEEACANI 310
Query: 253 ETL 261
E +
Sbjct: 311 ENV 313
Score = 40.7 bits (91), Expect = 0.034
Identities = 24/59 (40%), Positives = 33/59 (55%), Gaps = 1/59 (1%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQ-NGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SLE RVPFLD + + + P+ + + + KH LR+ F G +PD VLWR K
Sbjct: 395 AHSLEARVPFLDPRVIDAVMEVDPQYKTIEGEEKPEKHALRALF--DGEIPDPVLWRTK 451
>UniRef50_A0CN48 Cluster: Chromosome undetermined scaffold_22, whole
genome shotgun sequence; n=5; Oligohymenophorea|Rep:
Chromosome undetermined scaffold_22, whole genome
shotgun sequence - Paramecium tetraurelia
Length = 590
Score = 81.8 bits (193), Expect = 1e-14
Identities = 38/81 (46%), Positives = 56/81 (69%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH E+++ TTIRAS PMY++ + IK + +GEG+DEL GY+YF AP+ +
Sbjct: 318 VIYHTETFNNTTIRASTPMYMMCRRIKA-LGIKICLTGEGSDELFGGYLYFHKAPNRVEF 376
Query: 438 HKESVRLLSDIYLYDGLRADR 500
H+E +R L D++ YD LRA++
Sbjct: 377 HQELIRKLHDLHKYDLLRANK 397
Score = 37.1 bits (82), Expect = 0.41
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
K+ +F IG+ SPDL A+ VA++ G EHH +
Sbjct: 273 KVHSFCIGLEGSPDLHYAKKVAEFHGFEHHSFTY 306
>UniRef50_Q4TFB6 Cluster: Chromosome undetermined SCAF4706, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF4706,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 374
Score = 60.1 bits (139), Expect(2) = 4e-13
Identities = 26/40 (65%), Positives = 35/40 (87%), Gaps = 1/40 (2%)
Frame = +3
Query: 312 MYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYF-RDAPSE 428
M+L+SKYI+EK+D+ V+FSGEG+DEL QGYIYF + P+E
Sbjct: 325 MFLVSKYIREKSDSVVIFSGEGSDELTQGYIYFHKVTPAE 364
Score = 57.6 bits (133), Expect = 3e-07
Identities = 31/62 (50%), Positives = 38/62 (61%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A RKRLM+ RRI ++A + LAKE KL Y +QTF+IG DSPDL AAR
Sbjct: 159 AVRKRLMAQRRIGCLLSGGLDSSLVSATLMKLAKEEKLQYPLQTFSIGSEDSPDLLAARK 218
Query: 184 VA 189
V+
Sbjct: 219 VS 220
Score = 37.1 bits (82), Expect(2) = 4e-13
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMY 317
+VIYHLE+YDITTIRAS+ Y
Sbjct: 272 DVIYHLETYDITTIRASVREY 292
>UniRef50_Q01D26 Cluster: Asparagine synthetase; n=4; cellular
organisms|Rep: Asparagine synthetase - Ostreococcus
tauri
Length = 649
Score = 74.5 bits (175), Expect = 2e-12
Identities = 38/84 (45%), Positives = 57/84 (67%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VI+HLE+++ +RAS+PMYLLS++IK +V SGEG DEL GY+YF AP+ +
Sbjct: 335 DVIWHLETFE--QVRASVPMYLLSRHIKS-LGFKMVLSGEGADELFGGYLYFHKAPNPTE 391
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H E R ++ +D LRA+++T
Sbjct: 392 FHAECRRKTMRLHQWDVLRANKST 415
Score = 48.4 bits (110), Expect = 2e-04
Identities = 32/78 (41%), Positives = 40/78 (51%), Gaps = 4/78 (5%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIV---NYLAKE-YKLPYKIQTFAIGMGDSPDLA 171
A KRLM+D IT+I LAK Y + +F+IG+ +PDL
Sbjct: 247 AVTKRLMADVPYAVLLSGGLDSSLITSIAVRQRKLAKNTYGADEPVHSFSIGIKGAPDLV 306
Query: 172 AARTVADYLGTEHHEVQF 225
AAR VA+ LGT HHEV F
Sbjct: 307 AARKVAEQLGTIHHEVHF 324
>UniRef50_O16924 Cluster: Asparaginyl trna synthetase protein 2;
n=2; Caenorhabditis|Rep: Asparaginyl trna synthetase
protein 2 - Caenorhabditis elegans
Length = 551
Score = 73.3 bits (172), Expect = 5e-12
Identities = 34/83 (40%), Positives = 51/83 (61%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
V++ LE++D IR + YLL ++I + +D V+ SGEG DEL Y Y + AP+
Sbjct: 300 VVFALETFDPLIIRCGIAHYLLCQHISKSSDVKVLLSGEGADELFGSYAYMQRAPNALHL 359
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
HKE +R + ++ YD LR DR+T
Sbjct: 360 HKEILRRMHHLHQYDVLRCDRST 382
Score = 48.4 bits (110), Expect = 2e-04
Identities = 28/56 (50%), Positives = 35/56 (62%), Gaps = 1/56 (1%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLR-QPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
LE+RVPFLD +F +PP + P +L KH+LRS+F G LPD VLWR K
Sbjct: 387 LEIRVPFLDKRFIDLVSRLPPSYKLMPM--KLEKHVLRSAF--EGWLPDEVLWRSK 438
Score = 35.5 bits (78), Expect = 1.3
Identities = 22/66 (33%), Positives = 29/66 (43%)
Frame = +1
Query: 13 KRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAARTVAD 192
KRLM +R I +I K+ + F++G DSPDL A+ VAD
Sbjct: 223 KRLMGNRNFGFMLSGGLDSSLIASIATRFLKQKPI-----AFSVGFEDSPDLENAKKVAD 277
Query: 193 YLGTEH 210
YL H
Sbjct: 278 YLKIPH 283
>UniRef50_Q4C279 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=2; Chroococcales|Rep:
Asparagine synthase, glutamine-hydrolyzing -
Crocosphaera watsonii
Length = 500
Score = 66.5 bits (155), Expect = 6e-10
Identities = 31/83 (37%), Positives = 49/83 (59%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
+IY+LES+D +R+ +P Y S+ E V+ +GEG DEL GY Y++D PS+
Sbjct: 291 IIYYLESFDQDLVRSGIPCYFTSRLASEYVK--VILTGEGADELFAGYTYYKDIPSDDTL 348
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H+E R ++ ++ + R DR T
Sbjct: 349 HRELRRSVNSLHNINLQRVDRLT 371
Score = 47.6 bits (108), Expect = 3e-04
Identities = 30/72 (41%), Positives = 41/72 (56%)
Frame = +1
Query: 1 AACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAAR 180
A+ KRLMSD + I AI AK++K ++ TF++G+ S D+ AAR
Sbjct: 211 ASVVKRLMSDVPLGAFLSGGLDSSIIAAI----AKKHKS--ELHTFSVGIAGSKDINAAR 264
Query: 181 TVADYLGTEHHE 216
V+DYLGT HHE
Sbjct: 265 LVSDYLGTIHHE 276
Score = 37.9 bits (84), Expect = 0.24
Identities = 20/58 (34%), Positives = 32/58 (55%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A S+E RVPFLD++ +P L+ + K +LR +F +LP+ ++WR K
Sbjct: 373 AHSIEGRVPFLDLKMIELGQKVPAHLKLKGTPPVEKWILRKAF--EDILPEEIVWRKK 428
>UniRef50_A0ZA07 Cluster: Asparagine synthase B; n=1; Nodularia
spumigena CCY 9414|Rep: Asparagine synthase B -
Nodularia spumigena CCY 9414
Length = 496
Score = 65.7 bits (153), Expect = 1e-09
Identities = 33/84 (39%), Positives = 50/84 (59%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIY+LES+D +R+++P Y++S+ + V+ SGEG DEL GY YF D
Sbjct: 289 DVIYYLESFDPALVRSAIPCYIVSQLASKYVK--VILSGEGADELFAGYSYFADYDDAIA 346
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
HKESV ++ ++ + R DR T
Sbjct: 347 LHKESVTIIKGLHNLNLQRLDRMT 370
Score = 46.8 bits (106), Expect = 5e-04
Identities = 27/77 (35%), Positives = 43/77 (55%)
Frame = +1
Query: 10 RKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAARTVA 189
+KRLMSD + I A++ +P + +F++G+ +SPDL AAR VA
Sbjct: 213 QKRLMSDVPVGVFLSGGLDSSIIAALMRQ-----HIP-NLHSFSVGLPNSPDLKAARLVA 266
Query: 190 DYLGTEHHEVQFDENDI 240
++LGT HHE + E ++
Sbjct: 267 EHLGTIHHEYVYTEAEM 283
Score = 45.6 bits (103), Expect = 0.001
Identities = 27/58 (46%), Positives = 33/58 (56%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A LE RVPFLD F LSI P L+ + + K LLR +F S LLP ++WR K
Sbjct: 372 AHGLEGRVPFLDTDFIELSLSIDPTLKLYKTFGIEKWLLRQAF--SDLLPQEIVWRDK 427
>UniRef50_A5UW35 Cluster: Asparagine synthase; n=2; Roseiflexus|Rep:
Asparagine synthase - Roseiflexus sp. RS-1
Length = 498
Score = 59.7 bits (138), Expect = 7e-08
Identities = 30/82 (36%), Positives = 49/82 (59%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIY+LES D +R+++P Y L++ E+ V+ +GEG DEL GY Y R + +D
Sbjct: 288 DVIYYLESADPALVRSAVPNYFLARLASERVK--VILTGEGADELYAGYDYMRALTTPED 345
Query: 435 AHKESVRLLSDIYLYDGLRADR 500
H+E + +++ + RADR
Sbjct: 346 LHRELEIAIRELHRTNLQRADR 367
Score = 43.6 bits (98), Expect = 0.005
Identities = 19/30 (63%), Positives = 23/30 (76%)
Frame = +1
Query: 127 IQTFAIGMGDSPDLAAARTVADYLGTEHHE 216
I+TFA+GM S DL AAR +A YLGT H+E
Sbjct: 245 IETFAVGMEGSEDLEAARRMAQYLGTRHYE 274
Score = 42.7 bits (96), Expect = 0.008
Identities = 24/58 (41%), Positives = 34/58 (58%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
AF +E RVPFLD++ LS+PP+ + G K LLR +FA LP+ ++ R K
Sbjct: 371 AFGVEGRVPFLDVESIALALSLPPEWKLAAPGEPTKMLLREAFASD--LPESIVQRPK 426
>UniRef50_A1I7L9 Cluster: Asparagine synthase B; n=1; Candidatus
Desulfococcus oleovorans Hxd3|Rep: Asparagine synthase B
- Candidatus Desulfococcus oleovorans Hxd3
Length = 537
Score = 54.8 bits (126), Expect = 2e-06
Identities = 32/81 (39%), Positives = 46/81 (56%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VI LESYD + +R ++P Y K E TVV +GEG DEL GY Y + P +K
Sbjct: 297 VINKLESYDPSLVRCAVPCYFTCKMAAEYV--TVVLTGEGADELFTGYHYMKHFPEDK-L 353
Query: 438 HKESVRLLSDIYLYDGLRADR 500
+ E+ R + +++ + RADR
Sbjct: 354 NLEARRCIGNLHNINLQRADR 374
Score = 52.8 bits (121), Expect = 8e-06
Identities = 26/59 (44%), Positives = 36/59 (61%), Gaps = 2/59 (3%)
Frame = +2
Query: 512 FSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
FSLELRVPFLD + IP K+R+ ++ K +LR +F + LPD +LWR+K
Sbjct: 379 FSLELRVPFLDEAMVDLSMKIPHELKIREHNGAKIEKWILRKAFENTHYLPDDILWRYK 437
Score = 39.5 bits (88), Expect = 0.078
Identities = 27/91 (29%), Positives = 50/91 (54%), Gaps = 5/91 (5%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGD-----SPDL 168
A +KRL++D + +++V +A + ++P ++ TF +GM D S D+
Sbjct: 211 AVKKRLLADPEVPVGSFCSGGLD--SSLVAAIAAD-EIP-RLHTFVVGMKDAFGDVSDDV 266
Query: 169 AAARTVADYLGTEHHEVQFDENDIRKDLETL 261
AAR A ++G+ HHE+ F E++ + L T+
Sbjct: 267 KAARIAAAHIGSTHHELIFTEDEYYEALPTV 297
>UniRef50_Q3CG33 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=2; Thermoanaerobacter
ethanolicus|Rep: Asparagine synthase,
glutamine-hydrolyzing - Thermoanaerobacter ethanolicus
ATCC 33223
Length = 503
Score = 54.4 bits (125), Expect = 3e-06
Identities = 34/81 (41%), Positives = 45/81 (55%)
Frame = +1
Query: 10 RKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAARTVA 189
RKRL++D + I AI A +YK P + +FA+G+ S DL AR VA
Sbjct: 213 RKRLIADVPVGVFLSGGLDSSLIAAI----AAKYKNP--LHSFAVGVEGSNDLKNARVVA 266
Query: 190 DYLGTEHHEVQFDENDIRKDL 252
DY+GT HHE + E DI+K L
Sbjct: 267 DYVGTIHHEFIYTEEDIKKVL 287
Score = 48.8 bits (111), Expect = 1e-04
Identities = 28/83 (33%), Positives = 42/83 (50%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYHLES D +R+++ Y +SK V+ SGEG DEL GY Y ++ +
Sbjct: 290 VIYHLESCDPALVRSAVATYFVSKLASNYVK--VILSGEGADELFSGYHYLKNYTNPWKL 347
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
E + +++ + R DR T
Sbjct: 348 QSELKYITRNLHNTNLQRVDRMT 370
Score = 38.3 bits (85), Expect = 0.18
Identities = 20/58 (34%), Positives = 29/58 (50%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A S+E RVPFLDI+ + I P + + K +LR LP+ ++WR K
Sbjct: 372 AHSIEGRVPFLDIEVLRYAFKITPSFKINGREKTEKWILRK--LAENYLPESIVWRRK 427
>UniRef50_Q2AIS3 Cluster: Glutamine amidotransferase,
class-II:Asparagine synthase; n=1; Halothermothrix
orenii H 168|Rep: Glutamine amidotransferase,
class-II:Asparagine synthase - Halothermothrix orenii H
168
Length = 484
Score = 51.2 bits (117), Expect = 2e-05
Identities = 27/63 (42%), Positives = 36/63 (57%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIY LES+D +R+S+ Y + + KE T +V GEG DEL GY Y +D S D
Sbjct: 276 VIYQLESFDQYLVRSSIANYFVGRLAKE-TGVELVLCGEGGDELFGGYHYLKDMQSLADI 334
Query: 438 HKE 446
+E
Sbjct: 335 REE 337
Score = 42.7 bits (96), Expect = 0.008
Identities = 20/43 (46%), Positives = 29/43 (67%)
Frame = +1
Query: 97 LAKEYKLPYKIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQF 225
+A K+ I+TFA+G S DL AR VAD+LGT+H+E ++
Sbjct: 222 VAAASKVKEVIKTFAVGAKGSNDLEKARLVADHLGTDHYEYRY 264
Score = 36.3 bits (80), Expect = 0.72
Identities = 21/58 (36%), Positives = 30/58 (51%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SL +PF+D + SIP K + N + K +LR +F LPD ++WR K
Sbjct: 359 AHSLLYDMPFMDKEIVKFAFSIPVKWKLYGNKPVEKWILRKAFEDD--LPDEIIWRKK 414
>UniRef50_Q2CH15 Cluster: Asparagine synthetase B; n=1; Oceanicola
granulosus HTCC2516|Rep: Asparagine synthetase B -
Oceanicola granulosus HTCC2516
Length = 488
Score = 48.8 bits (111), Expect = 1e-04
Identities = 29/85 (34%), Positives = 42/85 (49%), Gaps = 1/85 (1%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRD-APSEK 431
+VIYHLES D+ +R++LP ++ + V +GEG DEL GY Y A +
Sbjct: 277 HVIYHLESADVDLVRSALPTLFAARL--ARAQVKAVLTGEGADELFAGYTYHHGYADDPR 334
Query: 432 DAHKESVRLLSDIYLYDGLRADRTT 506
E R L ++ + R DR T
Sbjct: 335 ALADELTRSLGTMHNINLQRVDRVT 359
Score = 47.2 bits (107), Expect = 4e-04
Identities = 19/38 (50%), Positives = 26/38 (68%)
Frame = +1
Query: 127 IQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDENDI 240
++TFA+G SPDL AAR VA ++G +HHE F D+
Sbjct: 234 LKTFAVGTDGSPDLRAARAVAAHIGADHHEAVFTAQDV 271
>UniRef50_Q4ZNC7 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Pseudomonas syringae pv.
syringae B728a|Rep: Asparagine synthase,
glutamine-hydrolyzing - Pseudomonas syringae pv.
syringae (strain B728a)
Length = 658
Score = 46.4 bits (105), Expect = 7e-04
Identities = 29/77 (37%), Positives = 43/77 (55%), Gaps = 4/77 (5%)
Frame = +3
Query: 261 IYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA--PSEKD 434
++H E T A +PMY+LSK+++ T + SGEG DE+ GY F++ S+ D
Sbjct: 332 VFHAEMPLFRT--APVPMYMLSKHVRS-TGIKAILSGEGADEVFLGYNIFKETLLRSQWD 388
Query: 435 A--HKESVRLLSDIYLY 479
H E RLL+ +Y Y
Sbjct: 389 TTDHDEKKRLLAKLYPY 405
>UniRef50_UPI000038414B Cluster: COG0367: Asparagine synthase
(glutamine-hydrolyzing); n=1; Magnetospirillum
magnetotacticum MS-1|Rep: COG0367: Asparagine synthase
(glutamine-hydrolyzing) - Magnetospirillum
magnetotacticum MS-1
Length = 498
Score = 44.4 bits (100), Expect = 0.003
Identities = 24/55 (43%), Positives = 32/55 (58%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAP 422
VI LES++ +R ++P+YLLSK + D VV G+G DEL GY Y P
Sbjct: 282 VIGALESFNPIMVRNAVPLYLLSKMAAK--DCKVVLGGDGADELFAGYDYLSRVP 334
Score = 34.3 bits (75), Expect = 2.9
Identities = 23/60 (38%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIP--PKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A LELRVPFLD + +++P K + K LR A GLLP + WR K
Sbjct: 363 AHGLELRVPFLDREVAEAAVNLPLSAKFGIKDGTPMPKLALRE--AAEGLLPPSIQWRRK 420
>UniRef50_Q1FIF7 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=3; Clostridiales|Rep:
Asparagine synthase, glutamine-hydrolyzing - Clostridium
phytofermentans ISDg
Length = 617
Score = 44.4 bits (100), Expect = 0.003
Identities = 25/55 (45%), Positives = 33/55 (60%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
LE RVPF DI+ + ++P ++ P + K LLR S SGLLPD +LWR K
Sbjct: 484 LEARVPFADIRIIEYLWNVPFSMKAPDG--IVKGLLRMSC--SGLLPDDILWRKK 534
>UniRef50_A0RRB3 Cluster: Asparagine synthase; n=1; Campylobacter
fetus subsp. fetus 82-40|Rep: Asparagine synthase -
Campylobacter fetus subsp. fetus (strain 82-40)
Length = 583
Score = 43.6 bits (98), Expect = 0.005
Identities = 28/62 (45%), Positives = 36/62 (58%), Gaps = 2/62 (3%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY-IYFRDAP-SEKDAHKESVRLLSDIY 473
A++P Y LSK I E V SGEG+DE GY +YFR +K+ +KES L+S Y
Sbjct: 329 ATIPTYFLSKKIHEN-GYKVALSGEGSDECFMGYDLYFRVLEFYQKNLNKESYPLISKDY 387
Query: 474 LY 479
Y
Sbjct: 388 EY 389
>UniRef50_A3CX83 Cluster: Asparagine synthase; n=1; Methanoculleus
marisnigri JR1|Rep: Asparagine synthase - Methanoculleus
marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Length = 603
Score = 43.6 bits (98), Expect = 0.005
Identities = 27/59 (45%), Positives = 34/59 (57%)
Frame = +2
Query: 500 NDEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWR 676
N AFS+E RVPFLD + + S+P L Q G + KH+LR A GL+PD V R
Sbjct: 474 NSMAFSIEARVPFLDYRLVEYLGSLP--LDQKIRGGVTKHVLRR--AIRGLVPDAVRCR 528
>UniRef50_Q67SU9 Cluster: Asparagine synthase B; n=1;
Symbiobacterium thermophilum|Rep: Asparagine synthase B
- Symbiobacterium thermophilum
Length = 506
Score = 43.2 bits (97), Expect = 0.006
Identities = 28/80 (35%), Positives = 39/80 (48%)
Frame = +1
Query: 1 AACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAAR 180
AA RK+L +D + +TAI A+ ++ TFA+G+ S DL AR
Sbjct: 211 AAVRKQLAADVPVGCLLSGGLDSSLVTAIARQHAEG-----ELHTFAVGLEGSADLEQAR 265
Query: 181 TVADYLGTEHHEVQFDENDI 240
VA LGT HHE E ++
Sbjct: 266 MVAGELGTIHHERVLTEKEV 285
Score = 34.7 bits (76), Expect = 2.2
Identities = 23/60 (38%), Positives = 32/60 (53%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
V+ LES D +R+++ Y +++ VV SGEG DEL GY Y A E+DA
Sbjct: 292 VVDALESCDPALVRSAVATYYVAELAAGHVK--VVLSGEGADELFAGYDYL--AEFERDA 347
>UniRef50_Q7MBG3 Cluster: PUTATIVE ASPARAGINE SYNTHETASE
(GLUTAMINE-HYDROLYZ (EC 6.3.5.4)); n=1; Wolinella
succinogenes|Rep: PUTATIVE ASPARAGINE SYNTHETASE
(GLUTAMINE-HYDROLYZ (EC 6.3.5.4)) - Wolinella
succinogenes
Length = 606
Score = 41.9 bits (94), Expect = 0.015
Identities = 21/56 (37%), Positives = 34/56 (60%)
Frame = +2
Query: 512 FSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRH 679
F +E R+P+LD + L++P +++ Q G L K LR + SGLLP ++WR+
Sbjct: 491 FGIETRLPYLDYRIVEWVLALPTEVKF-QQGYL-KFALREALEISGLLPKSIVWRY 544
>UniRef50_Q3EU47 Cluster: Asparagine synthetase; n=1; Bacillus
thuringiensis serovar israelensis ATCC 35646|Rep:
Asparagine synthetase - Bacillus thuringiensis serovar
israelensis ATCC 35646
Length = 646
Score = 41.1 bits (92), Expect = 0.025
Identities = 24/55 (43%), Positives = 34/55 (61%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
NVI HL++ I + A +PMYLL++++ V SGEG DEL GY F++A
Sbjct: 337 NVILHLQT-PILRLGA-IPMYLLAEFV-HNNGFKVALSGEGADELFGGYDIFKEA 388
>UniRef50_O26514 Cluster: Asparagine synthetase; n=1;
Methanothermobacter thermautotrophicus str. Delta H|Rep:
Asparagine synthetase - Methanobacterium
thermoautotrophicum
Length = 469
Score = 41.1 bits (92), Expect = 0.025
Identities = 26/60 (43%), Positives = 38/60 (63%), Gaps = 2/60 (3%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A S+ELRVPFLD+Q L+IP K+R P++ LRK +LR + + G +P+ + R K
Sbjct: 380 ANSVELRVPFLDLQLIGVALTIPADLKIRGPED-ELRKRILREAALEMG-VPEYIAMRPK 437
Score = 37.9 bits (84), Expect = 0.24
Identities = 19/53 (35%), Positives = 31/53 (58%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
+V+ +E Y++ I ++P+YL S+ V+FSG+G DEL GY +R
Sbjct: 296 HVLGAIEEYNLMKIGVAMPLYLASE-AASADGYRVMFSGQGADELFAGYHRYR 347
>UniRef50_Q2IZV9 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Rhodopseudomonas palustris
HaA2|Rep: Asparagine synthase, glutamine-hydrolyzing -
Rhodopseudomonas palustris (strain HaA2)
Length = 631
Score = 40.3 bits (90), Expect = 0.044
Identities = 24/56 (42%), Positives = 33/56 (58%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHKG 685
LE+R+PFLD + L +P K NG +RKH+LR SF +S L P+ + KG
Sbjct: 499 LEVRLPFLDSRLVAFALGLPDKFL--INGGVRKHILRESF-RSDLPPEILTRGKKG 551
>UniRef50_Q1VN43 Cluster: Asparagine synthase family protein; n=1;
Psychroflexus torquis ATCC 700755|Rep: Asparagine
synthase family protein - Psychroflexus torquis ATCC
700755
Length = 399
Score = 39.9 bits (89), Expect = 0.059
Identities = 24/84 (28%), Positives = 44/84 (52%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+++Y ES++ + + L YLL++ + + V+ GEG DEL GY F+ A +
Sbjct: 201 DIVYATESFNPSIVSNGLGSYLLARAARNE-GIKVILGGEGADELFGGYHSFQSASEPWE 259
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
+ RL+ D++ + R D T+
Sbjct: 260 LTR--ARLIEDMHRTELRRLDLTS 281
Score = 35.9 bits (79), Expect = 0.96
Identities = 23/61 (37%), Positives = 33/61 (54%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHKGS 688
A S+E+R PFLD + + S + KH+LR +F +GLLPD +L R K S
Sbjct: 283 AHSVEVRCPFLDREMRAY--SDKLRFEDIYGASGNKHILRMTF--NGLLPDSILMRKKTS 338
Query: 689 I 691
+
Sbjct: 339 L 339
>UniRef50_Q8TPE7 Cluster: Asparagine synthase; n=3;
Methanosarcina|Rep: Asparagine synthase - Methanosarcina
acetivorans
Length = 497
Score = 39.9 bits (89), Expect = 0.059
Identities = 19/48 (39%), Positives = 29/48 (60%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
V+Y ES D T+ +P+Y+++K KE V+ +G+G DEL GY
Sbjct: 302 VVYATESTDPMTVAIGIPLYIVAKTAKE-DGKRVLLTGQGADELFGGY 348
Score = 36.7 bits (81), Expect = 0.55
Identities = 26/65 (40%), Positives = 38/65 (58%), Gaps = 7/65 (10%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLR-QPQNGRL------RKHLLRSSFAKSGLLPDCV 667
A S+ELRVPFLD + L+I P+L+ ++G RK++LR A GL+P +
Sbjct: 387 ANSVELRVPFLDKEVIKTGLAIRPELKVLKKDGYYEDGYYERKYILRK--AAKGLIPPEI 444
Query: 668 LWRHK 682
LW+ K
Sbjct: 445 LWKEK 449
>UniRef50_Q12VP2 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Methanococcoides burtonii
DSM 6242|Rep: Asparagine synthase, glutamine-hydrolyzing
- Methanococcoides burtonii (strain DSM 6242)
Length = 634
Score = 39.9 bits (89), Expect = 0.059
Identities = 22/87 (25%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = +1
Query: 10 RKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPD---LAAAR 180
+ RLMS+ + +T +++ + E I+TF++G G+ + L+ A+
Sbjct: 244 KMRLMSEVPLGIYLSGGIDSGIVTGLMSKMVDE-----PIETFSVGFGERGEIGELSQAQ 298
Query: 181 TVADYLGTEHHEVQFDENDIRKDLETL 261
AD+ GT HHE+ D+ +++L+ +
Sbjct: 299 EAADFFGTNHHEIIVDQGAFKQNLKDI 325
>UniRef50_A5UZF1 Cluster: Asparagine synthase; n=9; cellular
organisms|Rep: Asparagine synthase - Roseiflexus sp.
RS-1
Length = 664
Score = 39.5 bits (88), Expect = 0.078
Identities = 21/54 (38%), Positives = 35/54 (64%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
VI+H E+ + T A P++LLS+ ++++ + VV +GEG DE GY F++A
Sbjct: 330 VIWHTETPILRTSPA--PLFLLSQLVRDR-NYRVVLTGEGADEFLAGYDIFKEA 380
>UniRef50_Q60BD5 Cluster: Asparagine synthetase,
glutamine-hydrolyzing; n=3; Gammaproteobacteria|Rep:
Asparagine synthetase, glutamine-hydrolyzing -
Methylococcus capsulatus
Length = 606
Score = 38.7 bits (86), Expect = 0.14
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +3
Query: 282 DITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEK 431
D+ ASLP LL++ E + VVFSGEG DE+ GY +R +P E+
Sbjct: 335 DLMRDNASLPTSLLARAAGE--ELKVVFSGEGGDEVFAGYGRYRTSPLER 382
>UniRef50_A5V1J9 Cluster: Asparagine synthase; n=5;
Chloroflexaceae|Rep: Asparagine synthase - Roseiflexus
sp. RS-1
Length = 665
Score = 38.7 bits (86), Expect = 0.14
Identities = 23/59 (38%), Positives = 27/59 (45%)
Frame = +2
Query: 500 NDEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWR 676
N AFS+E RVPFLD + P LR + H A GLLPD + WR
Sbjct: 537 NSMAFSIEARVPFLDHRLVEFVFQCAPHLRIRRGWTKWVH----RQAMEGLLPDAIAWR 591
>UniRef50_UPI00015BCA27 Cluster: UPI00015BCA27 related cluster; n=1;
unknown|Rep: UPI00015BCA27 UniRef100 entry - unknown
Length = 640
Score = 38.3 bits (85), Expect = 0.18
Identities = 19/39 (48%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY-IYFR 413
A+LP Y++S YIK+ V SGEG+DEL GY +Y++
Sbjct: 358 ATLPTYVISSYIKQN-GIKVALSGEGSDELFFGYDLYYK 395
>UniRef50_Q982D6 Cluster: Mlr9125 protein; n=1; Mesorhizobium
loti|Rep: Mlr9125 protein - Rhizobium loti
(Mesorhizobium loti)
Length = 520
Score = 38.3 bits (85), Expect = 0.18
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +1
Query: 79 TAIVNYLAKEYKLPYKIQTFAIGMG-DSPDLAAARTVADYLGTEHHEV 219
+A++ + + +I TF +G G D P++ A A LGT+HHE+
Sbjct: 254 SAVIAATLRRIRRDSEIHTFTVGYGYDDPEIIGASATASVLGTQHHEI 301
>UniRef50_Q30U64 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Thiomicrospira denitrificans
ATCC 33889|Rep: Asparagine synthase,
glutamine-hydrolyzing - Thiomicrospira denitrificans
(strain ATCC 33889 / DSM 1351)
Length = 583
Score = 38.3 bits (85), Expect = 0.18
Identities = 25/87 (28%), Positives = 45/87 (51%), Gaps = 3/87 (3%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSP---DLAA 174
A +KRL+ D + ITA+ AK+ K +K+ TF+IG + +L
Sbjct: 236 AVQKRLVGDVEVATLLSGGLDSSFITALY---AKKSK--HKVHTFSIGYDEHKHYCELGF 290
Query: 175 ARTVADYLGTEHHEVQFDENDIRKDLE 255
A+ ++Y+GT HHE + +++ + +E
Sbjct: 291 AKAASEYIGTIHHEYKISKDEYLEAIE 317
>UniRef50_A3I8W6 Cluster: Asparagine synthetase; n=4; Bacteria|Rep:
Asparagine synthetase - Bacillus sp. B14905
Length = 636
Score = 38.3 bits (85), Expect = 0.18
Identities = 17/40 (42%), Positives = 28/40 (70%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
+S+P+Y LS+ + D TVV SGEG DE+ GY +++++
Sbjct: 360 SSVPLYFLSELASK--DVTVVLSGEGADEIFGGYSWYQNS 397
>UniRef50_A7IB45 Cluster: Asparagine synthase; n=1; Candidatus
Methanoregula boonei 6A8|Rep: Asparagine synthase -
Methanoregula boonei (strain 6A8)
Length = 607
Score = 38.3 bits (85), Expect = 0.18
Identities = 25/59 (42%), Positives = 33/59 (55%)
Frame = +2
Query: 500 NDEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWR 676
N AFSLE RVP+LD++ + S+P L Q G K LR+ A GL+P+ V R
Sbjct: 473 NSMAFSLESRVPYLDVRLVEYAASLP--LSQKIRGGCTKVALRA--AIRGLVPESVRCR 527
>UniRef50_Q58516 Cluster: Putative asparagine synthetase
[glutamine-hydrolyzing] 1; n=7; Methanococcales|Rep:
Putative asparagine synthetase [glutamine-hydrolyzing] 1
- Methanococcus jannaschii
Length = 541
Score = 38.3 bits (85), Expect = 0.18
Identities = 21/68 (30%), Positives = 32/68 (47%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKES 449
++ D+ I +P+Y+ S+ E VV SG+G DEL GY E+ +
Sbjct: 332 IDEVDLMKIGVGIPIYVASEMANED-GLKVVLSGQGADELFGGYARHERIYRERGEEELK 390
Query: 450 VRLLSDIY 473
LL D+Y
Sbjct: 391 KELLKDVY 398
>UniRef50_Q7M7N7 Cluster: WBFR PROTEIN; n=1; Wolinella
succinogenes|Rep: WBFR PROTEIN - Wolinella succinogenes
Length = 604
Score = 37.9 bits (84), Expect = 0.24
Identities = 24/58 (41%), Positives = 30/58 (51%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SLE R PFLD Q LS+P LR G + K+LL+ LPD ++ R K
Sbjct: 483 AHSLESRAPFLDYQLVDFVLSLPSSLR---TGEITKNLLKK--IALAYLPDSIVNRRK 535
Score = 35.1 bits (77), Expect = 1.7
Identities = 19/40 (47%), Positives = 26/40 (65%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
A +P YLLSK + K+ VV SGEG+DE+ GY + D+
Sbjct: 334 AIIPTYLLSKEVA-KSGFKVVLSGEGSDEIFFGYDGYFDS 372
>UniRef50_Q3AVQ8 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=13; Bacteria|Rep: Asparagine
synthase, glutamine-hydrolyzing - Synechococcus sp.
(strain CC9902)
Length = 674
Score = 37.9 bits (84), Expect = 0.24
Identities = 24/60 (40%), Positives = 33/60 (55%)
Frame = +2
Query: 503 DEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
D A S+E R FLD + +PP+LR G+ K++LR + A GLLPD + R K
Sbjct: 516 DMANSMEARPAFLDHHLAAVAVQVPPELR--IKGKTEKYVLREAMA--GLLPDVLYKREK 571
Score = 33.1 bits (72), Expect = 6.7
Identities = 18/51 (35%), Positives = 30/51 (58%)
Frame = +3
Query: 261 IYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
++H E T+ ++ +L+S+++ D VV +GEG+DEL GY FR
Sbjct: 342 LWHTERTIYNTL--AVAKFLMSRHVNS-VDYKVVMTGEGSDELFGGYPAFR 389
>UniRef50_Q30U87 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Thiomicrospira denitrificans
ATCC 33889|Rep: Asparagine synthase,
glutamine-hydrolyzing - Thiomicrospira denitrificans
(strain ATCC 33889 / DSM 1351)
Length = 601
Score = 37.9 bits (84), Expect = 0.24
Identities = 25/59 (42%), Positives = 34/59 (57%), Gaps = 1/59 (1%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY-IYFRDAPSEKDAHKESVRLLSDIY 473
A++P+YLL +IK K VV SGEG+DEL GY YF EK + ++ LS +
Sbjct: 331 AAVPLYLLFGHIK-KDGYKVVLSGEGSDELFLGYRQYFEFLDIEKLTNLKNKNWLSGYF 388
>UniRef50_A0YEF5 Cluster: Asparagine synthase; n=1; marine gamma
proteobacterium HTCC2143|Rep: Asparagine synthase -
marine gamma proteobacterium HTCC2143
Length = 638
Score = 37.9 bits (84), Expect = 0.24
Identities = 21/46 (45%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +3
Query: 315 YLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR-DAPSEKDAHKES 449
YLLSK +++ VVF+GEG DE+ GY +FR DA A E+
Sbjct: 346 YLLSKAVRD-AGIKVVFTGEGADEMLGGYPFFRVDAVKHNPALSEA 390
>UniRef50_P54420 Cluster: Asparagine synthetase
[glutamine-hydrolyzing] 1; n=34; Bacilli|Rep: Asparagine
synthetase [glutamine-hydrolyzing] 1 - Bacillus subtilis
Length = 632
Score = 37.9 bits (84), Expect = 0.24
Identities = 21/58 (36%), Positives = 33/58 (56%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEK 431
+++H + D A++P+Y ++K + K TV SGEG DEL GY +R+ S K
Sbjct: 329 IVWHFD--DPLADPAAIPLYFVAK--EAKKHVTVALSGEGADELFGGYNIYREPLSLK 382
Score = 35.1 bits (77), Expect = 1.7
Identities = 27/58 (46%), Positives = 34/58 (58%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SLELRVPFLD IP +L+ +NG K+LLR A G++P+ VL R K
Sbjct: 491 ANSLELRVPFLDKVVFDVASKIPDELK-TKNG-TTKYLLRK--AAEGIVPEHVLNRKK 544
>UniRef50_Q2SJG5 Cluster: Asparagine synthase; n=1; Hahella
chejuensis KCTC 2396|Rep: Asparagine synthase - Hahella
chejuensis (strain KCTC 2396)
Length = 662
Score = 37.5 bits (83), Expect = 0.31
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +3
Query: 306 LPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
+P YLLSK+ +EK TV SG+G DEL GY
Sbjct: 359 IPTYLLSKFTREKV--TVALSGDGGDELFAGY 388
>UniRef50_Q1QD48 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Psychrobacter cryohalolentis
K5|Rep: Asparagine synthase, glutamine-hydrolyzing -
Psychrobacter cryohalolentis (strain K5)
Length = 630
Score = 37.5 bits (83), Expect = 0.31
Identities = 23/59 (38%), Positives = 30/59 (50%)
Frame = +2
Query: 500 NDEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWR 676
N AFS+E RVPFL + LS+P G + KH+ R A G++PD L R
Sbjct: 499 NSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKG-VTKHIFRE--AMRGIVPDSHLDR 554
>UniRef50_Q1NS49 Cluster: Asparagine synthase; n=1; delta
proteobacterium MLMS-1|Rep: Asparagine synthase - delta
proteobacterium MLMS-1
Length = 583
Score = 37.5 bits (83), Expect = 0.31
Identities = 18/39 (46%), Positives = 25/39 (64%), Gaps = 1/39 (2%)
Frame = +1
Query: 124 KIQTFAIG-MGDSPDLAAARTVADYLGTEHHEVQFDEND 237
K+QTF + ++PDL AAR VA+ L T+HHE +D
Sbjct: 282 KLQTFTLADSNETPDLLAARQVAEALKTDHHEYIVTSDD 320
Score = 34.3 bits (75), Expect = 2.9
Identities = 17/43 (39%), Positives = 24/43 (55%)
Frame = +3
Query: 294 IRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAP 422
I+ L ++L++YI + V FSGEG DEL GY + P
Sbjct: 343 IQGGLAFHILAQYISKHVK--VAFSGEGADELFGGYYWIHTHP 383
>UniRef50_Q01Z46 Cluster: Asparagine synthase; n=1; Solibacter
usitatus Ellin6076|Rep: Asparagine synthase - Solibacter
usitatus (strain Ellin6076)
Length = 639
Score = 37.5 bits (83), Expect = 0.31
Identities = 24/58 (41%), Positives = 32/58 (55%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SLE+R PFLD + S+P +L+ G +KH+LR G LP+ VL R K
Sbjct: 494 AHSLEVRPPFLDHRIVEFAASLPQRLK--IRGAKQKHVLRE--LMRGKLPESVLTRKK 547
>UniRef50_A7H9Q9 Cluster: Asparagine synthase; n=3;
Proteobacteria|Rep: Asparagine synthase -
Anaeromyxobacter sp. Fw109-5
Length = 676
Score = 37.5 bits (83), Expect = 0.31
Identities = 26/80 (32%), Positives = 41/80 (51%), Gaps = 5/80 (6%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAP---- 422
+V+ H E + T A P++LLSK +++ VV +GEG DE+ GY F++
Sbjct: 333 DVVAHAERPLLRT--APAPLFLLSKLVRD-AGIKVVLTGEGADEVFAGYDLFKEGKVRRF 389
Query: 423 -SEKDAHKESVRLLSDIYLY 479
+ A + RLL +Y Y
Sbjct: 390 WGRQPASRLRPRLLERLYPY 409
>UniRef50_O05272 Cluster: Asparagine synthetase
[glutamine-hydrolyzing] 3; n=38; Firmicutes|Rep:
Asparagine synthetase [glutamine-hydrolyzing] 3 -
Bacillus subtilis
Length = 614
Score = 37.5 bits (83), Expect = 0.31
Identities = 22/56 (39%), Positives = 33/56 (58%)
Frame = +2
Query: 515 SLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
SLE+RVPF D + + +IP +++ N K +LR A G+LPD +L+R K
Sbjct: 478 SLEVRVPFADHRLVEYVWNIPWEMKMHDN--REKGILRK--ALEGILPDDILYRKK 529
>UniRef50_A4B944 Cluster: Asparagine synthase; n=1; Reinekea sp.
MED297|Rep: Asparagine synthase - Reinekea sp. MED297
Length = 657
Score = 37.1 bits (82), Expect = 0.41
Identities = 17/49 (34%), Positives = 32/49 (65%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKE 446
+++P YLL++++K+K TV +G+G DEL GY ++ A ++ K+
Sbjct: 356 SAIPTYLLTEFVKKKV--TVCMAGDGGDELFLGYNRYQKASQLRNLSKD 402
>UniRef50_Q0W279 Cluster: Putative asparagine synthetase,
glutamine-hydrolyzing; n=1; uncultured methanogenic
archaeon RC-I|Rep: Putative asparagine synthetase,
glutamine-hydrolyzing - Uncultured methanogenic archaeon
RC-I
Length = 452
Score = 37.1 bits (82), Expect = 0.41
Identities = 19/48 (39%), Positives = 29/48 (60%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
VIY +ES ++ +LP+Y+LS + V+ SG+G+DEL GY
Sbjct: 275 VIYAIESASPVSVSIALPLYILSARAR-ADGYKVLLSGQGSDELFAGY 321
>UniRef50_Q2LWL8 Cluster: Asparagine synthetase; n=1; Syntrophus
aciditrophicus SB|Rep: Asparagine synthetase -
Syntrophus aciditrophicus (strain SB)
Length = 645
Score = 36.7 bits (81), Expect = 0.55
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3
Query: 303 SLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
S+P+Y ++K+ KE+ TV+ +GEG DEL GY +R
Sbjct: 356 SVPLYFVTKFAKER-GVTVLQAGEGADELFCGYDNYR 391
Score = 33.9 bits (74), Expect = 3.9
Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 4/52 (7%)
Frame = +1
Query: 118 PYKIQTFAIGMGDS----PDLAAARTVADYLGTEHHEVQFDENDIRKDLETL 261
P K++TF IGM ++ D AR +A L ++HHE++ +E D+ E L
Sbjct: 292 PEKVKTFTIGMDNAGSFHDDSTIAREMALRLCSDHHEMRINEADLLAAAEKL 343
>UniRef50_Q8TZ36 Cluster: Asparagine synthase; n=1; Methanopyrus
kandleri|Rep: Asparagine synthase - Methanopyrus
kandleri
Length = 520
Score = 36.7 bits (81), Expect = 0.55
Identities = 25/58 (43%), Positives = 34/58 (58%), Gaps = 2/58 (3%)
Frame = +2
Query: 515 SLELRVPFLDIQFTHHYLSIPPK--LRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
S+ELRVP+LD+ L I P+ + P++ LRK LR A+ G LPD V+ R K
Sbjct: 375 SVELRVPYLDLDVVRTGLGIDPRENVSGPED-NLRKRALRRVAAELG-LPDFVVERRK 430
>UniRef50_Q62A30 Cluster: Asparagine synthase; n=24; Bacteria|Rep:
Asparagine synthase - Burkholderia mallei (Pseudomonas
mallei)
Length = 622
Score = 36.3 bits (80), Expect = 0.72
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAH 440
L ++D+ A L + L + + + TV SGE DE+ GY++F D P+ + AH
Sbjct: 350 LRAWDLPFNFADLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSD-PAARRAH 405
>UniRef50_Q0LLW6 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Herpetosiphon aurantiacus
ATCC 23779|Rep: Asparagine synthase,
glutamine-hydrolyzing - Herpetosiphon aurantiacus ATCC
23779
Length = 622
Score = 36.3 bits (80), Expect = 0.72
Identities = 18/47 (38%), Positives = 29/47 (61%), Gaps = 2/47 (4%)
Frame = +1
Query: 127 IQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQFDENDIRKDLETL 261
I TF++G + +L A VA + GT+HHE++ + ND+ L+TL
Sbjct: 283 INTFSVGFEHAGFNELPDAALVAQHFGTKHHELRLNANDLVGALQTL 329
Score = 33.9 bits (74), Expect = 3.9
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A S+E RVPFLD +++PP L+ G K LLR +F + +LP L + K
Sbjct: 492 AASIEARVPFLDHTLVEWTMNLPPTLK--LRGTKTKWLLRQAFGE--MLPQRTLRKPK 545
Score = 33.5 bits (73), Expect = 5.1
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
A LP+YL+S++ +E VV +GEG+DE GY
Sbjct: 341 AGLPVYLVSRFARE--HVKVVLTGEGSDEQWAGY 372
>UniRef50_A6Q4Y9 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Nitratiruptor sp.
SB155-2|Rep: Asparagine synthase, glutamine-hydrolyzing
- Nitratiruptor sp. (strain SB155-2)
Length = 567
Score = 36.3 bits (80), Expect = 0.72
Identities = 22/64 (34%), Positives = 36/64 (56%), Gaps = 2/64 (3%)
Frame = +1
Query: 85 IVNYLAKEYKLPYKIQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQFDENDIRKDLET 258
+ ++L+K+Y L +TF IG D + A+ A LGT+HHE+ F +++ K L T
Sbjct: 261 LASFLSKKYPL----KTFTIGFEDERFNEAKIAKATARKLGTQHHELYFGIDELLKLLPT 316
Query: 259 LFTI 270
+ I
Sbjct: 317 VADI 320
>UniRef50_A6DEM6 Cluster: Probable asparagine synthase,
glutamine-hydrolyzing; n=1; Caminibacter mediatlanticus
TB-2|Rep: Probable asparagine synthase,
glutamine-hydrolyzing - Caminibacter mediatlanticus TB-2
Length = 630
Score = 36.3 bits (80), Expect = 0.72
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +1
Query: 121 YKIQTFAIGMGDS--PDLAAARTVADYLGTEHHEVQFDENDIRKDLETL 261
YK+ TF IG D + + A+ VA+Y T H+E+ +E + K +E L
Sbjct: 282 YKLNTFTIGFDDKRYDEASHAKIVANYFKTNHNELYINETMMLKKIEKL 330
>UniRef50_Q2SJH5 Cluster: Asparagine synthase; n=1; Hahella
chejuensis KCTC 2396|Rep: Asparagine synthase - Hahella
chejuensis (strain KCTC 2396)
Length = 610
Score = 35.9 bits (79), Expect = 0.96
Identities = 20/52 (38%), Positives = 24/52 (46%)
Frame = +1
Query: 79 TAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEN 234
+AIV + K + F G GD DL AR +A Y G H E FD N
Sbjct: 257 SAIVAMMKKHTSHTIRTYAFGSGPGDE-DLCRARKMAKYFGVRHKEFYFDAN 307
>UniRef50_Q08YT4 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Stigmatella aurantiaca
DW4/3-1|Rep: Asparagine synthase, glutamine-hydrolyzing
- Stigmatella aurantiaca DW4/3-1
Length = 525
Score = 35.9 bits (79), Expect = 0.96
Identities = 16/44 (36%), Positives = 28/44 (63%), Gaps = 1/44 (2%)
Frame = +1
Query: 124 KIQTFAIGMGDS-PDLAAARTVADYLGTEHHEVQFDENDIRKDL 252
+I TF++ + PDLA +R +++ LGT HHE F+ + + + L
Sbjct: 223 EIHTFSLADDPTLPDLAVSRRLSEALGTHHHEYSFEPDTLLESL 266
>UniRef50_A0A004 Cluster: MoeF5; n=1; Streptomyces ghanaensis|Rep:
MoeF5 - Streptomyces ghanaensis
Length = 645
Score = 35.9 bits (79), Expect = 0.96
Identities = 27/89 (30%), Positives = 39/89 (43%), Gaps = 3/89 (3%)
Frame = +1
Query: 1 AACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAA-- 174
A R+ +DRR+ + A+ L +P TF+ G D PD
Sbjct: 247 AVARRMTSTDRRLGVLLSGGLDSSAVAAVAQQLLPGRPVP----TFSAGFAD-PDFDESD 301
Query: 175 -ARTVADYLGTEHHEVQFDENDIRKDLET 258
AR VA +LGTEHH V+ D+ +E+
Sbjct: 302 HARAVARHLGTEHHVVRIGGADLAGVVES 330
>UniRef50_Q4FTY4 Cluster: Probable asparagine synthase,
glutamine-hydrolyzing; n=1; Psychrobacter arcticus|Rep:
Probable asparagine synthase, glutamine-hydrolyzing -
Psychrobacter arcticum
Length = 623
Score = 35.5 bits (78), Expect = 1.3
Identities = 24/61 (39%), Positives = 34/61 (55%), Gaps = 5/61 (8%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYF-----RDAPSEKDAHKESVRLLS 464
+++P L+SK KEK +V+ SG+G DEL GY + R A K K S++ LS
Sbjct: 347 SAIPTTLVSKLAKEKV--SVILSGDGGDELFCGYSSYTLMEKRFASISKIPFKNSIKKLS 404
Query: 465 D 467
D
Sbjct: 405 D 405
>UniRef50_Q319R2 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Prochlorococcus marinus str.
MIT 9312|Rep: Asparagine synthase, glutamine-hydrolyzing
- Prochlorococcus marinus (strain MIT 9312)
Length = 622
Score = 35.5 bits (78), Expect = 1.3
Identities = 21/86 (24%), Positives = 42/86 (48%), Gaps = 2/86 (2%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSP--DLAAA 177
+C R +SD I +T+I+ +K+ +I+TF IG D + A
Sbjct: 251 SCLSRTISDVPIACFLSGGIDSSLVTSIIQRNSKQ-----RIKTFTIGFDDQSIDESNYA 305
Query: 178 RTVADYLGTEHHEVQFDENDIRKDLE 255
+A++LGT+H + +E ++ +++
Sbjct: 306 EKIANFLGTDHTTINIEEKELLNNID 331
>UniRef50_A7DMN1 Cluster: Asparagine synthase; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: Asparagine synthase -
Candidatus Nitrosopumilus maritimus SCM1
Length = 339
Score = 35.5 bits (78), Expect = 1.3
Identities = 21/65 (32%), Positives = 38/65 (58%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKES 449
L++++ IR ++ MYL K+ K+ + +++ +G+G DEL GY F SE++ E
Sbjct: 119 LKNFNDIEIRNNVVMYLAIKWAKDNGEKSII-TGDGADELFAGY-NFLVHKSEEELESEI 176
Query: 450 VRLLS 464
R+ S
Sbjct: 177 NRVCS 181
>UniRef50_A0RUC0 Cluster: Asparagine synthase; n=1; Cenarchaeum
symbiosum|Rep: Asparagine synthase - Cenarchaeum
symbiosum
Length = 383
Score = 35.5 bits (78), Expect = 1.3
Identities = 22/62 (35%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYF-RDAPSEKDAHKE 446
L S++ IR S MYLL + + T T + +G+G DEL GY +F R P+E + ++
Sbjct: 162 LGSFNDIEIRNSAAMYLLIERVAG-TGWTSMITGDGADELFAGYDFFGRLGPAEIEEEQQ 220
Query: 447 SV 452
+
Sbjct: 221 RI 222
>UniRef50_Q216T1 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Rhodopseudomonas palustris
BisB18|Rep: Asparagine synthase, glutamine-hydrolyzing -
Rhodopseudomonas palustris (strain BisB18)
Length = 592
Score = 35.1 bits (77), Expect = 1.7
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +3
Query: 330 YIKEKTDTTVVFSGEGTDELAQGYIYFRD 416
Y + K + TVV SGEG DEL GY +F D
Sbjct: 354 YREVKPNVTVVMSGEGADELFAGYHFFLD 382
>UniRef50_A6Q5J5 Cluster: Putative uncharacterized protein; n=1;
Nitratiruptor sp. SB155-2|Rep: Putative uncharacterized
protein - Nitratiruptor sp. (strain SB155-2)
Length = 571
Score = 35.1 bits (77), Expect = 1.7
Identities = 18/34 (52%), Positives = 22/34 (64%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
AS+P+Y L K IK K +V SGEG DE+ GY
Sbjct: 306 ASVPLYFLMKRIK-KDGFRIVLSGEGGDEIFLGY 338
>UniRef50_A4TWX4 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Magnetospirillum
gryphiswaldense|Rep: Asparagine synthase,
glutamine-hydrolyzing - Magnetospirillum gryphiswaldense
Length = 657
Score = 35.1 bits (77), Expect = 1.7
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +3
Query: 261 IYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRD 416
+YH E + A +PM+LLS+ ++ V+ SGEG DE GY F++
Sbjct: 332 VYHAEMPAFRS--AFIPMFLLSRLTRDN-GIKVILSGEGADEAFLGYDLFKE 380
>UniRef50_A4BPQ6 Cluster: Asparagine synthase; n=1; Nitrococcus
mobilis Nb-231|Rep: Asparagine synthase - Nitrococcus
mobilis Nb-231
Length = 631
Score = 35.1 bits (77), Expect = 1.7
Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Frame = +1
Query: 127 IQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQFDEND 237
++T+A+G GD P + A +A+YLGT+H E+ D
Sbjct: 278 VKTYALGFGDGPVSETPYASAIANYLGTDHTELHVTGKD 316
>UniRef50_A1ASZ8 Cluster: Asparagine synthase; n=2;
Desulfuromonadales|Rep: Asparagine synthase - Pelobacter
propionicus (strain DSM 2379)
Length = 678
Score = 35.1 bits (77), Expect = 1.7
Identities = 20/53 (37%), Positives = 31/53 (58%)
Frame = +3
Query: 261 IYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
I+H E+ + T A +P+++LS + + VV GEG DEL GY F++A
Sbjct: 332 IWHCETPILRT--APVPLFILSSLVNTE-GFKVVLCGEGADELFGGYNIFKEA 381
>UniRef50_Q31I32 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=4; Proteobacteria|Rep:
Asparagine synthase, glutamine-hydrolyzing -
Thiomicrospira crunogena (strain XCL-2)
Length = 602
Score = 34.7 bits (76), Expect = 2.2
Identities = 20/51 (39%), Positives = 30/51 (58%), Gaps = 4/51 (7%)
Frame = +3
Query: 303 SLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYF----RDAPSEKDAHK 443
++ YLLS+ + E D VV SG+G DE+ GY ++ + A S KDA +
Sbjct: 350 AIAFYLLSEKVSE--DVKVVMSGQGADEVFAGYFWYPMMAKAAESLKDASR 398
>UniRef50_Q12IB8 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Shewanella denitrificans
OS217|Rep: Asparagine synthase, glutamine-hydrolyzing -
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 /
DSM 15013)
Length = 621
Score = 34.7 bits (76), Expect = 2.2
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHKGS 688
A S+E R PFLDI + +IPP+L +NG K+LL+ K+ +P+ ++ R K S
Sbjct: 490 ANSVEARYPFLDIDLINFVRTIPPEL-MLKNGN-EKYLLKQLATKT--VPESIIRRKKFS 545
>UniRef50_A6PQS0 Cluster: Asparagine synthase; n=1; Victivallis
vadensis ATCC BAA-548|Rep: Asparagine synthase -
Victivallis vadensis ATCC BAA-548
Length = 633
Score = 34.3 bits (75), Expect = 2.9
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = +3
Query: 306 LPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
LP YLLS++ +E TV SG+G DE+ GY
Sbjct: 351 LPTYLLSRFAREHI--TVALSGDGADEVFSGY 380
>UniRef50_A3ZYU5 Cluster: Asparagine synthetase
[glutamine-hydrolyzing] 1; n=2; Planctomycetaceae|Rep:
Asparagine synthetase [glutamine-hydrolyzing] 1 -
Blastopirellula marina DSM 3645
Length = 638
Score = 34.3 bits (75), Expect = 2.9
Identities = 16/34 (47%), Positives = 23/34 (67%), Gaps = 2/34 (5%)
Frame = +1
Query: 127 IQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQ 222
++TF+IG + + AR VA++LGTEHHE Q
Sbjct: 294 VKTFSIGFPQKEYDETSFARQVAEHLGTEHHEFQ 327
>UniRef50_Q9UZJ0 Cluster: Putative putative asparagine synthetase;
n=1; Pyrococcus abyssi|Rep: Putative putative asparagine
synthetase - Pyrococcus abyssi
Length = 611
Score = 34.3 bits (75), Expect = 2.9
Identities = 20/54 (37%), Positives = 30/54 (55%)
Frame = +2
Query: 500 NDEAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPD 661
N +S+E RVPF D + + LS+PP+ + + + K LLR G+LPD
Sbjct: 479 NAMRWSIESRVPFCDHELVEYVLSLPPESK--VSAGITKVLLRKGL--KGILPD 528
>UniRef50_Q9HL64 Cluster: Hypothetical membrane protein; n=1;
Thermoplasma acidophilum|Rep: Hypothetical membrane
protein - Thermoplasma acidophilum
Length = 618
Score = 34.3 bits (75), Expect = 2.9
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +3
Query: 273 ESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKESV 452
E+Y +T R +LPM L + I K V F + D + IYFR+ + H V
Sbjct: 467 ENYRLTLYRKTLPMLLKLEKIDVKEVNNVKFVNDKPD-IKDTKIYFRNGENPSGLHTYLV 525
Query: 453 RLLSDIYLYDGL 488
+D+ + G+
Sbjct: 526 NRTADVIYHKGI 537
>UniRef50_Q1N9Z8 Cluster: Putative uncharacterized protein; n=1;
Sphingomonas sp. SKA58|Rep: Putative uncharacterized
protein - Sphingomonas sp. SKA58
Length = 761
Score = 33.9 bits (74), Expect = 3.9
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +1
Query: 100 AKEYKLPYKIQTFAIGMGDSPDLAAARTVADYLGTEH 210
A + ++ +I+ F G D+PD+AAA VA+ G +H
Sbjct: 268 ATQSRIADRIEAFTYGFPDNPDIAAAAIVAEKAGVKH 304
>UniRef50_Q02LN1 Cluster: Asparagine synthetase,
glutamine-hydrolysing; n=4; Pseudomonas aeruginosa|Rep:
Asparagine synthetase, glutamine-hydrolysing -
Pseudomonas aeruginosa (strain UCBPP-PA14)
Length = 610
Score = 33.9 bits (74), Expect = 3.9
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = +3
Query: 312 MYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRD 416
+YLL + ++E T TV SGE DE+ GY +F D
Sbjct: 359 LYLLFRQVREHT--TVALSGESADEVFGGYPFFHD 391
>UniRef50_Q02CD2 Cluster: Asparagine synthase; n=1; Solibacter
usitatus Ellin6076|Rep: Asparagine synthase - Solibacter
usitatus (strain Ellin6076)
Length = 644
Score = 33.9 bits (74), Expect = 3.9
Identities = 22/63 (34%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Frame = +1
Query: 79 TAIVNYLAKEYKLPYKIQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQFDENDIRKDL 252
+AI + + + P K TFA+G ++ +L+ AR VA +GT+HHEV D L
Sbjct: 271 SAIAALMKRNFSGPVK--TFAVGYQEAEFSELSYARHVAGAIGTDHHEVVVGMEDFFNAL 328
Query: 253 ETL 261
L
Sbjct: 329 PRL 331
Score = 33.9 bits (74), Expect = 3.9
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
+S+ +Y +SK +E TVV +GEG+DE+ GY +R
Sbjct: 343 SSVSLYFVSKLAREHV--TVVLTGEGSDEMFGGYARYR 378
Score = 32.7 bits (71), Expect = 8.9
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A S+E RVPFLD +F +P ++ +NG K++++ A GL+P +++R K
Sbjct: 501 ATSIESRVPFLDHEFVEFSTRVPEHMKL-RNGE-GKYIVKK--AIEGLVPHEIIYRKK 554
>UniRef50_Q01UW3 Cluster: Asparagine synthase; n=1; Solibacter
usitatus Ellin6076|Rep: Asparagine synthase - Solibacter
usitatus (strain Ellin6076)
Length = 632
Score = 33.9 bits (74), Expect = 3.9
Identities = 17/53 (32%), Positives = 31/53 (58%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
+ ++H ES+ + S + LS+ +++ VV +GEG+DE+ GY +FR
Sbjct: 329 DALWHNESFFLNA--GSAAKFALSRAVRD-AGYKVVLTGEGSDEVLAGYAFFR 378
>UniRef50_A6DB45 Cluster: Putative uncharacterized protein; n=1;
Caminibacter mediatlanticus TB-2|Rep: Putative
uncharacterized protein - Caminibacter mediatlanticus
TB-2
Length = 140
Score = 33.9 bits (74), Expect = 3.9
Identities = 19/57 (33%), Positives = 32/57 (56%)
Frame = +3
Query: 303 SLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKESVRLLSDIY 473
++P+YL+ ++IKE D V+F G G DE G I F + ++ E+ L+D +
Sbjct: 85 NIPLYLIIEFIKEFCD-NVLFLGIGVDEKNLGKITFEISNKALNSLNEAFNKLNDSF 140
>UniRef50_A2EQ39 Cluster: Asparagine synthase family protein; n=1;
Trichomonas vaginalis G3|Rep: Asparagine synthase family
protein - Trichomonas vaginalis G3
Length = 410
Score = 33.9 bits (74), Expect = 3.9
Identities = 24/82 (29%), Positives = 42/82 (51%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
++I ++ + T + ++ M L + I+ T T V SG G DEL GY+ + A +D
Sbjct: 254 SIIQNVMPLETTEMNLNIAMTLYNGLIRSPTPT--VLSGLGPDELFCGYMSMKSA---ED 308
Query: 435 AHKESVRLLSDIYLYDGLRADR 500
KE ++ ++ +G R DR
Sbjct: 309 VDKEVCMYINRLWERNGGRDDR 330
>UniRef50_Q6W388 Cluster: Asparagine synthase; n=1; uncultured
crenarchaeote DeepAnt-EC39|Rep: Asparagine synthase -
uncultured crenarchaeote DeepAnt-EC39
Length = 315
Score = 33.9 bits (74), Expect = 3.9
Identities = 17/44 (38%), Positives = 27/44 (61%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
L++++ IR S+ YL +KEK T V+ +G+G DE+ GY
Sbjct: 94 LKNFNDIEIRNSIVPYLYMNALKEKNITNVI-TGDGADEIFAGY 136
>UniRef50_P34303 Cluster: Putative zinc finger protein C06E1.8; n=2;
Caenorhabditis|Rep: Putative zinc finger protein C06E1.8
- Caenorhabditis elegans
Length = 397
Score = 33.9 bits (74), Expect = 3.9
Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 3/55 (5%)
Frame = +1
Query: 304 VCQCTFCQSILKKKQTQQWYLAEKEPMN*RKAISTSE---THRLKRTHIKRAYVC 459
+C FC S + Q + + RK IS+SE TH K+ HI R Y+C
Sbjct: 55 ICSKVFCHSSSLSRHRMQAHFKSYKCTVCRKDISSSESLRTHMFKQHHISRMYMC 109
>UniRef50_Q0IDQ7 Cluster: Asparagine synthase; n=1; Synechococcus
sp. CC9311|Rep: Asparagine synthase - Synechococcus sp.
(strain CC9311)
Length = 604
Score = 33.5 bits (73), Expect = 5.1
Identities = 20/58 (34%), Positives = 31/58 (53%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
A SLE+R PFLD + + S+P K++ G K++ + A LL + + WR K
Sbjct: 467 AHSLEVRSPFLDYRLVEYAFSLPGKMK--LKGTETKYIYK--MAVEELLGETLTWRKK 520
>UniRef50_A5ZSA8 Cluster: Putative uncharacterized protein; n=1;
Ruminococcus obeum ATCC 29174|Rep: Putative
uncharacterized protein - Ruminococcus obeum ATCC 29174
Length = 585
Score = 33.5 bits (73), Expect = 5.1
Identities = 21/59 (35%), Positives = 33/59 (55%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEK 431
+V+YH++ + +++ +Y LSK + VV SGEG DEL GY +R+ S K
Sbjct: 324 DVMYHMD--EPLGDASAVALYFLSK--EAAGHVKVVLSGEGADELFGGYNIYREPESLK 378
>UniRef50_O28833 Cluster: Asparagine synthetase; n=1; Archaeoglobus
fulgidus|Rep: Asparagine synthetase - Archaeoglobus
fulgidus
Length = 371
Score = 33.5 bits (73), Expect = 5.1
Identities = 20/67 (29%), Positives = 35/67 (52%)
Frame = +3
Query: 270 LESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKES 449
+E+ D + ++P+YL ++ K T ++F G+G DEL GY + + SE +
Sbjct: 219 IETGDFLQLSIAVPVYLTMQFAKSLGFTEIIF-GQGADELFGGYKRY-EGLSEHELEDAL 276
Query: 450 VRLLSDI 470
V+ L I
Sbjct: 277 VKDLKSI 283
>UniRef50_UPI0000384B0F Cluster: COG0367: Asparagine synthase
(glutamine-hydrolyzing); n=1; Magnetospirillum
magnetotacticum MS-1|Rep: COG0367: Asparagine synthase
(glutamine-hydrolyzing) - Magnetospirillum
magnetotacticum MS-1
Length = 637
Score = 33.1 bits (72), Expect = 6.7
Identities = 22/54 (40%), Positives = 31/54 (57%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVL 670
A S+E RVP LD + L++PP +R P G +K L R+ A+ LPD +L
Sbjct: 491 AHSVEGRVPLLDHRMVEAALAVPPHIRNP--GGRQKALERAIAAE--YLPDSLL 540
>UniRef50_Q5FML5 Cluster: Asn synthetase; n=20; Bacteria|Rep: Asn
synthetase - Lactobacillus acidophilus
Length = 649
Score = 33.1 bits (72), Expect = 6.7
Identities = 17/49 (34%), Positives = 27/49 (55%)
Frame = +3
Query: 264 YHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYF 410
YH++ D + +P++ L K ++ TV SGEG DEL GY+ +
Sbjct: 324 YHMDEPDANP--SIIPLWYLCKLARKHV--TVALSGEGADELFAGYVNY 368
>UniRef50_Q11P23 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Cytophaga hutchinsonii ATCC
33406|Rep: Asparagine synthase, glutamine-hydrolyzing -
Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
Length = 615
Score = 33.1 bits (72), Expect = 6.7
Identities = 24/73 (32%), Positives = 38/73 (52%), Gaps = 5/73 (6%)
Frame = +3
Query: 270 LESYDIT-TIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQG----YIYFRDAPSEKD 434
+E YD + + LP L+SK ++ T TV +G+G DEL G Y Y R ++
Sbjct: 325 IEFYDEPFAVSSVLPSLLISKVSRQHT--TVALTGDGADELFMGYNTYYWYNRIETLKRI 382
Query: 435 AHKESVRLLSDIY 473
K S++L +I+
Sbjct: 383 GGKASLKLAGNIF 395
>UniRef50_A4X6Q9 Cluster: Asparagine synthase; n=1; Salinispora
tropica CNB-440|Rep: Asparagine synthase - Salinispora
tropica CNB-440
Length = 536
Score = 33.1 bits (72), Expect = 6.7
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +1
Query: 142 IGMGDSPDLAAARTVADYLGTEHHEVQFDENDIR 243
+G+ +SPDL AR +A LG H V+ DIR
Sbjct: 259 VGVPESPDLLYARRLAKDLGVPHEVVELQPRDIR 292
>UniRef50_A0M2W8 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=1; Gramella forsetii
KT0803|Rep: Asparagine synthetase
[glutamine-hydrolyzing] - Gramella forsetii (strain
KT0803)
Length = 633
Score = 33.1 bits (72), Expect = 6.7
Identities = 16/57 (28%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +1
Query: 97 LAKEYKLPYKIQTFAIGMGDSP--DLAAARTVADYLGTEHHEVQFDENDIRKDLETL 261
LA ++ KI TF++G + +RTVA+ + + HHE +D++++L+ +
Sbjct: 281 LALSQQVQGKIDTFSVGFEKKSFDETDKSRTVANLINSNHHEFIISASDLKENLDEI 337
>UniRef50_A0LYW1 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=1; Gramella forsetii
KT0803|Rep: Asparagine synthetase
[glutamine-hydrolyzing] - Gramella forsetii (strain
KT0803)
Length = 573
Score = 33.1 bits (72), Expect = 6.7
Identities = 17/40 (42%), Positives = 24/40 (60%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLR 628
++S ELR PFLD + SIP ++ NG+ K+LLR
Sbjct: 445 SYSTELREPFLDYRLVELAFSIPDNMK--INGKTHKYLLR 482
>UniRef50_A5UJI7 Cluster: Asparagine synthetase, AsnB; n=1;
Methanobrevibacter smithii ATCC 35061|Rep: Asparagine
synthetase, AsnB - Methanobrevibacter smithii (strain PS
/ ATCC 35061 / DSM 861)
Length = 475
Score = 33.1 bits (72), Expect = 6.7
Identities = 16/43 (37%), Positives = 27/43 (62%), Gaps = 2/43 (4%)
Frame = +1
Query: 79 TAIVNYLAKEYKLP--YKIQTFAIGMGDSPDLAAARTVADYLG 201
++++ L +E L K+ +A+G DS D+ AA+ VA+YLG
Sbjct: 245 SSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLG 287
Score = 32.7 bits (71), Expect = 8.9
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLR-QPQNGRLRKHLLRSSFAKSGL 652
A +ELR+PFLD L+IP + + + +LRK++LR + GL
Sbjct: 390 ANGVELRLPFLDKNLVEFALNIPVRYKISGSDDKLRKNILRKTAFNLGL 438
>UniRef50_Q5FPJ6 Cluster: Asparagine synthetase
[glutamine-hydrolyzing]; n=5; Rhodospirillales|Rep:
Asparagine synthetase [glutamine-hydrolyzing] -
Gluconobacter oxydans (Gluconobacter suboxydans)
Length = 586
Score = 32.7 bits (71), Expect = 8.9
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFR 413
A +P + L++ + + D TV+ SGEG DEL GY +R
Sbjct: 342 AVIPTWFLAR--EARKDVTVILSGEGGDELFAGYGRYR 377
>UniRef50_Q2ILL8 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=2; Proteobacteria|Rep:
Asparagine synthase, glutamine-hydrolyzing -
Anaeromyxobacter dehalogenans (strain 2CP-C)
Length = 646
Score = 32.7 bits (71), Expect = 8.9
Identities = 25/61 (40%), Positives = 29/61 (47%), Gaps = 4/61 (6%)
Frame = +3
Query: 252 GNVIYHLE----SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDA 419
G+ + HLE S D T L Y LS I+ TV +G G DEL GY FRD
Sbjct: 330 GHFVEHLEAALDSLDQPTFDG-LNSYYLSHAIRS-AGFTVALAGTGGDELFGGYTSFRDL 387
Query: 420 P 422
P
Sbjct: 388 P 388
>UniRef50_Q6QW19 Cluster: Asparagine synthase-like protein; n=1;
Azospirillum brasilense|Rep: Asparagine synthase-like
protein - Azospirillum brasilense
Length = 553
Score = 32.7 bits (71), Expect = 8.9
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGR--LRKHL 622
A+ LE+RVPFLD + LS PP + +G+ LR++L
Sbjct: 436 AYGLEVRVPFLDHRLVEWGLSRPPAAEEAVHGKPVLRRYL 475
>UniRef50_Q220Y1 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=3; Betaproteobacteria|Rep:
Asparagine synthase, glutamine-hydrolyzing - Rhodoferax
ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118)
Length = 619
Score = 32.7 bits (71), Expect = 8.9
Identities = 20/53 (37%), Positives = 33/53 (62%), Gaps = 6/53 (11%)
Frame = +1
Query: 79 TAIVNYLAKEYKLPYKIQTFAIGM--GDSP----DLAAARTVADYLGTEHHEV 219
+A+V ++AK + P I+T+AIG G++ +L AR VA+ GT+H E+
Sbjct: 246 SAVVGFMAKHSQQP--IRTYAIGFSGGEAEALYNELPYARRVAELFGTQHREI 296
>UniRef50_A6GFB8 Cluster: Asparagine synthase,
glutamine-hydrolyzing; n=1; Plesiocystis pacifica
SIR-1|Rep: Asparagine synthase, glutamine-hydrolyzing -
Plesiocystis pacifica SIR-1
Length = 930
Score = 32.7 bits (71), Expect = 8.9
Identities = 16/31 (51%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = +1
Query: 127 IQTFAIGMG-DSPDLAAARTVADYLGTEHHE 216
+ TF + + PDL AART+A LGT HHE
Sbjct: 306 VHTFTLADDPEHPDLEAARTLAAKLGTVHHE 336
>UniRef50_A3T3D5 Cluster: Asparagine synthase; n=1; Sulfitobacter
sp. NAS-14.1|Rep: Asparagine synthase - Sulfitobacter
sp. NAS-14.1
Length = 641
Score = 32.7 bits (71), Expect = 8.9
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGY 401
+ LP YL+S+ +E TV SG+G DEL GY
Sbjct: 339 SQLPTYLVSRMARESV--TVALSGDGGDELFAGY 370
>UniRef50_A0Q7S4 Cluster: Glutamine amidotransferase/asparagine
synthase; n=10; Francisella tularensis|Rep: Glutamine
amidotransferase/asparagine synthase - Francisella
tularensis subsp. novicida (strain U112)
Length = 630
Score = 32.7 bits (71), Expect = 8.9
Identities = 18/42 (42%), Positives = 24/42 (57%)
Frame = +3
Query: 300 ASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPS 425
+ +P YL+SK K K TV SG+ DEL GY + AP+
Sbjct: 350 SQIPTYLVSKIAKSKV--TVALSGDAGDELFGGYNRYFLAPN 389
>UniRef50_Q58456 Cluster: Putative asparagine synthetase
[glutamine-hydrolyzing] 2; n=3; Methanococcales|Rep:
Putative asparagine synthetase [glutamine-hydrolyzing] 2
- Methanococcus jannaschii
Length = 515
Score = 32.7 bits (71), Expect = 8.9
Identities = 15/42 (35%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +1
Query: 124 KIQTFAIGM-GDSPDLAAARTVADYLGTEHHEVQFDENDIRK 246
K+ TF+IG G + + V DY T+HH F E D +
Sbjct: 300 KLHTFSIGFEGKYDETPYIKIVVDYFKTQHHHYYFKERDFEE 341
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 675,809,567
Number of Sequences: 1657284
Number of extensions: 13259414
Number of successful extensions: 32117
Number of sequences better than 10.0: 115
Number of HSP's better than 10.0 without gapping: 30796
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32068
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 55371905986
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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