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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30650
         (700 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0)               130   1e-30
SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33)         101   7e-22
SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0)                 28   8.4  

>SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0)
          Length = 1663

 Score =  130 bits (314), Expect = 1e-30
 Identities = 59/84 (70%), Positives = 71/84 (84%)
 Frame = +3

Query: 255  NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
            +VIY LESYDITTIRAS+PMYL++KY+ E TDT VV+SGEG+DEL QGYIYF  APS ++
Sbjct: 1459 DVIYALESYDITTIRASVPMYLVAKYVSENTDTVVVYSGEGSDELCQGYIYFHKAPSPEE 1518

Query: 435  AHKESVRLLSDIYLYDGLRADRTT 506
              KES+RLL D+YLYD LR DR+T
Sbjct: 1519 GDKESLRLLKDLYLYDVLRGDRST 1542



 Score = 72.1 bits (169), Expect = 4e-13
 Identities = 36/74 (48%), Positives = 47/74 (63%)
 Frame = +1

Query: 4    ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
            A +KRLM+DRRI            + A++   AK   + Y IQTF+IGM  SPD+AAAR 
Sbjct: 1375 AVKKRLMADRRIGCFLSGGLDSSLVAALLTKEAKNAGIDYPIQTFSIGMEGSPDVAAARK 1434

Query: 184  VADYLGTEHHEVQF 225
            VA ++G+EHHEV F
Sbjct: 1435 VAAHIGSEHHEVTF 1448



 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 31/55 (56%), Positives = 42/55 (76%)
 Frame = +2

Query: 518  LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
            LE+RVPFLD+ FT ++LS+PP  RQP+ G + K LLR++F  +GLLP  +LWR K
Sbjct: 1547 LEIRVPFLDVAFTSYFLSLPPAERQPKEG-VEKWLLRAAFDGTGLLPHEILWRPK 1600


>SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33)
          Length = 274

 Score =  101 bits (241), Expect = 7e-22
 Identities = 47/84 (55%), Positives = 60/84 (71%)
 Frame = +3

Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
           +VIY LE YD   I  S+ +YLLSKY+ E T+T V++ GEG DEL QGY+YF  APS + 
Sbjct: 35  DVIYTLEIYDFDQICPSVALYLLSKYVHENTETVVLYCGEGADELCQGYVYFHKAPSAEM 94

Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
           A +ES RLL D+YL+D +R DR T
Sbjct: 95  ADQESRRLLKDLYLFDLVRVDRAT 118



 Score = 63.3 bits (147), Expect = 2e-10
 Identities = 30/58 (51%), Positives = 41/58 (70%)
 Frame = +2

Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
           AF LE RVPFLD+ FT  +LS+P   RQP+ G + K LLR+++  +G+LP  +LWR K
Sbjct: 120 AFGLEARVPFLDVPFTSFFLSLPAVERQPRGG-VEKWLLRAAYDGTGILPGEILWRTK 176



 Score = 37.5 bits (83), Expect = 0.010
 Identities = 18/39 (46%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
 Frame = +1

Query: 157 SPDLAAARTVADYLGTEHHEVQF-DENDIRKDLETLFTI 270
           S DL AAR VA+++G+EHHEV F  E  I+   + ++T+
Sbjct: 2   STDLVAARKVAEHIGSEHHEVIFTPEEGIQALRDVIYTL 40


>SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0)
          Length = 942

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = +2

Query: 530 VPFLDIQFTHHYLSIPPKLRQPQ 598
           V FL    T H+ S+ PKL QPQ
Sbjct: 478 VGFLSFDSTVHFYSLKPKLTQPQ 500


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,277,571
Number of Sequences: 59808
Number of extensions: 426139
Number of successful extensions: 1054
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1052
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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