BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30650
(700 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0) 130 1e-30
SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33) 101 7e-22
SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0) 28 8.4
>SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0)
Length = 1663
Score = 130 bits (314), Expect = 1e-30
Identities = 59/84 (70%), Positives = 71/84 (84%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIY LESYDITTIRAS+PMYL++KY+ E TDT VV+SGEG+DEL QGYIYF APS ++
Sbjct: 1459 DVIYALESYDITTIRASVPMYLVAKYVSENTDTVVVYSGEGSDELCQGYIYFHKAPSPEE 1518
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
KES+RLL D+YLYD LR DR+T
Sbjct: 1519 GDKESLRLLKDLYLYDVLRGDRST 1542
Score = 72.1 bits (169), Expect = 4e-13
Identities = 36/74 (48%), Positives = 47/74 (63%)
Frame = +1
Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183
A +KRLM+DRRI + A++ AK + Y IQTF+IGM SPD+AAAR
Sbjct: 1375 AVKKRLMADRRIGCFLSGGLDSSLVAALLTKEAKNAGIDYPIQTFSIGMEGSPDVAAARK 1434
Query: 184 VADYLGTEHHEVQF 225
VA ++G+EHHEV F
Sbjct: 1435 VAAHIGSEHHEVTF 1448
Score = 67.3 bits (157), Expect = 1e-11
Identities = 31/55 (56%), Positives = 42/55 (76%)
Frame = +2
Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
LE+RVPFLD+ FT ++LS+PP RQP+ G + K LLR++F +GLLP +LWR K
Sbjct: 1547 LEIRVPFLDVAFTSYFLSLPPAERQPKEG-VEKWLLRAAFDGTGLLPHEILWRPK 1600
>SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33)
Length = 274
Score = 101 bits (241), Expect = 7e-22
Identities = 47/84 (55%), Positives = 60/84 (71%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIY LE YD I S+ +YLLSKY+ E T+T V++ GEG DEL QGY+YF APS +
Sbjct: 35 DVIYTLEIYDFDQICPSVALYLLSKYVHENTETVVLYCGEGADELCQGYVYFHKAPSAEM 94
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
A +ES RLL D+YL+D +R DR T
Sbjct: 95 ADQESRRLLKDLYLFDLVRVDRAT 118
Score = 63.3 bits (147), Expect = 2e-10
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682
AF LE RVPFLD+ FT +LS+P RQP+ G + K LLR+++ +G+LP +LWR K
Sbjct: 120 AFGLEARVPFLDVPFTSFFLSLPAVERQPRGG-VEKWLLRAAYDGTGILPGEILWRTK 176
Score = 37.5 bits (83), Expect = 0.010
Identities = 18/39 (46%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
Frame = +1
Query: 157 SPDLAAARTVADYLGTEHHEVQF-DENDIRKDLETLFTI 270
S DL AAR VA+++G+EHHEV F E I+ + ++T+
Sbjct: 2 STDLVAARKVAEHIGSEHHEVIFTPEEGIQALRDVIYTL 40
>SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0)
Length = 942
Score = 27.9 bits (59), Expect = 8.4
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +2
Query: 530 VPFLDIQFTHHYLSIPPKLRQPQ 598
V FL T H+ S+ PKL QPQ
Sbjct: 478 VGFLSFDSTVHFYSLKPKLTQPQ 500
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,277,571
Number of Sequences: 59808
Number of extensions: 426139
Number of successful extensions: 1054
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1052
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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