BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30650 (700 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0) 130 1e-30 SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33) 101 7e-22 SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0) 28 8.4 >SB_46550| Best HMM Match : Asn_synthase (HMM E-Value=0) Length = 1663 Score = 130 bits (314), Expect = 1e-30 Identities = 59/84 (70%), Positives = 71/84 (84%) Frame = +3 Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434 +VIY LESYDITTIRAS+PMYL++KY+ E TDT VV+SGEG+DEL QGYIYF APS ++ Sbjct: 1459 DVIYALESYDITTIRASVPMYLVAKYVSENTDTVVVYSGEGSDELCQGYIYFHKAPSPEE 1518 Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506 KES+RLL D+YLYD LR DR+T Sbjct: 1519 GDKESLRLLKDLYLYDVLRGDRST 1542 Score = 72.1 bits (169), Expect = 4e-13 Identities = 36/74 (48%), Positives = 47/74 (63%) Frame = +1 Query: 4 ACRKRLMSDRRIXXXXXXXXXXXXITAIVNYLAKEYKLPYKIQTFAIGMGDSPDLAAART 183 A +KRLM+DRRI + A++ AK + Y IQTF+IGM SPD+AAAR Sbjct: 1375 AVKKRLMADRRIGCFLSGGLDSSLVAALLTKEAKNAGIDYPIQTFSIGMEGSPDVAAARK 1434 Query: 184 VADYLGTEHHEVQF 225 VA ++G+EHHEV F Sbjct: 1435 VAAHIGSEHHEVTF 1448 Score = 67.3 bits (157), Expect = 1e-11 Identities = 31/55 (56%), Positives = 42/55 (76%) Frame = +2 Query: 518 LELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682 LE+RVPFLD+ FT ++LS+PP RQP+ G + K LLR++F +GLLP +LWR K Sbjct: 1547 LEIRVPFLDVAFTSYFLSLPPAERQPKEG-VEKWLLRAAFDGTGLLPHEILWRPK 1600 >SB_32549| Best HMM Match : Asn_synthase (HMM E-Value=3.4e-33) Length = 274 Score = 101 bits (241), Expect = 7e-22 Identities = 47/84 (55%), Positives = 60/84 (71%) Frame = +3 Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434 +VIY LE YD I S+ +YLLSKY+ E T+T V++ GEG DEL QGY+YF APS + Sbjct: 35 DVIYTLEIYDFDQICPSVALYLLSKYVHENTETVVLYCGEGADELCQGYVYFHKAPSAEM 94 Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506 A +ES RLL D+YL+D +R DR T Sbjct: 95 ADQESRRLLKDLYLFDLVRVDRAT 118 Score = 63.3 bits (147), Expect = 2e-10 Identities = 30/58 (51%), Positives = 41/58 (70%) Frame = +2 Query: 509 AFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGRLRKHLLRSSFAKSGLLPDCVLWRHK 682 AF LE RVPFLD+ FT +LS+P RQP+ G + K LLR+++ +G+LP +LWR K Sbjct: 120 AFGLEARVPFLDVPFTSFFLSLPAVERQPRGG-VEKWLLRAAYDGTGILPGEILWRTK 176 Score = 37.5 bits (83), Expect = 0.010 Identities = 18/39 (46%), Positives = 27/39 (69%), Gaps = 1/39 (2%) Frame = +1 Query: 157 SPDLAAARTVADYLGTEHHEVQF-DENDIRKDLETLFTI 270 S DL AAR VA+++G+EHHEV F E I+ + ++T+ Sbjct: 2 STDLVAARKVAEHIGSEHHEVIFTPEEGIQALRDVIYTL 40 >SB_52129| Best HMM Match : Sec23_trunk (HMM E-Value=0) Length = 942 Score = 27.9 bits (59), Expect = 8.4 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = +2 Query: 530 VPFLDIQFTHHYLSIPPKLRQPQ 598 V FL T H+ S+ PKL QPQ Sbjct: 478 VGFLSFDSTVHFYSLKPKLTQPQ 500 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,277,571 Number of Sequences: 59808 Number of extensions: 426139 Number of successful extensions: 1054 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 978 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1052 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1829596184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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