Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30650
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identica... 94 7e-20
At3g47340.3 68416.m05147 asparagine synthetase 1 [glutamine-hydr... 94 7e-20
At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydr... 94 7e-20
At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydr... 94 7e-20
At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identica... 93 1e-19
At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identica... 93 1e-19
At3g16550.1 68416.m02113 DegP protease, putative contains simila... 29 3.9
At4g02870.1 68417.m00387 expressed protein 27 9.0
At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family pro... 27 9.0
>At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identical
to asparagine synthetase (ASN3) [Arabidopsis thaliana]
GI:3859534
Length = 578
Score = 94.3 bits (224), Expect = 7e-20
Identities = 42/83 (50%), Positives = 63/83 (75%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+
Sbjct: 306 VIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEIFGGYLYFHKAPNKKEF 364
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ YD LRA+++T
Sbjct: 365 HEETCRKIKALHQYDCLRANKST 387
Score = 53.2 bits (122), Expect = 2e-07
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
K+ TF IG+ SPDL A R VADYLGT HHE+ F D
Sbjct: 261 KLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQD 298
Score = 45.6 bits (103), Expect = 3e-05
Identities = 26/62 (41%), Positives = 40/62 (64%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A+ +E RVPFLD +F + +SI P K+ +P GR+ K +LR++F K+ LP +L+R
Sbjct: 389 AWGVEARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEKNPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
>At3g47340.3 68416.m05147 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 456
Score = 94.3 bits (224), Expect = 7e-20
Identities = 42/84 (50%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG DE+ GY+YF AP++K+
Sbjct: 305 DVIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKE 363
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ YD LRA+++T
Sbjct: 364 FHQETCRKIKALHKYDCLRANKST 387
Score = 45.6 bits (103), Expect = 3e-05
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ +F +G+ SPDL A + VA+YLGT HHE F D
Sbjct: 261 QLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 298
Score = 29.9 bits (64), Expect = 1.7
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPPK 583
AF LE RVPFLD F + +S+ P+
Sbjct: 389 AFGLEARVPFLDKDFINTAMSLDPE 413
>At3g47340.2 68416.m05146 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 512
Score = 94.3 bits (224), Expect = 7e-20
Identities = 42/84 (50%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG DE+ GY+YF AP++K+
Sbjct: 305 DVIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKE 363
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ YD LRA+++T
Sbjct: 364 FHQETCRKIKALHKYDCLRANKST 387
Score = 46.4 bits (105), Expect = 2e-05
Identities = 26/62 (41%), Positives = 38/62 (61%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
AF LE RVPFLD F + +S+ P K+ +P+ GR+ K +LR +F + LP +L+R
Sbjct: 389 AFGLEARVPFLDKDFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
Score = 45.6 bits (103), Expect = 3e-05
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ +F +G+ SPDL A + VA+YLGT HHE F D
Sbjct: 261 QLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 298
>At3g47340.1 68416.m05145 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 584
Score = 94.3 bits (224), Expect = 7e-20
Identities = 42/84 (50%), Positives = 63/84 (75%)
Frame = +3
Query: 255 NVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKD 434
+VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG DE+ GY+YF AP++K+
Sbjct: 305 DVIYHVETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKE 363
Query: 435 AHKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ YD LRA+++T
Sbjct: 364 FHQETCRKIKALHKYDCLRANKST 387
Score = 46.4 bits (105), Expect = 2e-05
Identities = 26/62 (41%), Positives = 38/62 (61%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
AF LE RVPFLD F + +S+ P K+ +P+ GR+ K +LR +F + LP +L+R
Sbjct: 389 AFGLEARVPFLDKDFINTAMSLDPESKMIKPEEGRIEKWVLRRAFDDEERPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
Score = 45.6 bits (103), Expect = 3e-05
Identities = 18/38 (47%), Positives = 25/38 (65%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ +F +G+ SPDL A + VA+YLGT HHE F D
Sbjct: 261 QLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQD 298
>At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identical
to asparagine synthetase (ASN2) [Arabidopsis thaliana]
GI:3859536
Length = 579
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/83 (49%), Positives = 63/83 (75%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+
Sbjct: 306 VIYHIETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEILGGYLYFHKAPNKKEF 364
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ +D LRA+++T
Sbjct: 365 HEETCRKIKALHQFDCLRANKST 387
Score = 52.8 bits (121), Expect = 2e-07
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ TF IG+ SPDL A R VADYLGT HHE QF D
Sbjct: 261 QLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQD 298
Score = 44.0 bits (99), Expect = 1e-04
Identities = 26/62 (41%), Positives = 39/62 (62%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A+ +E RVPFLD +F + +SI P KL +P GR+ K +LR++F + LP +L+R
Sbjct: 389 AWGVEARVPFLDKEFLNVAMSIDPEWKLIKPDLGRIEKWVLRNAFDDEERPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
>At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identical
to asparagine synthetase (ASN2) [Arabidopsis thaliana]
GI:3859536
Length = 578
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/83 (49%), Positives = 63/83 (75%)
Frame = +3
Query: 258 VIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDA 437
VIYH+E+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+
Sbjct: 306 VIYHIETYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEILGGYLYFHKAPNKKEF 364
Query: 438 HKESVRLLSDIYLYDGLRADRTT 506
H+E+ R + ++ +D LRA+++T
Sbjct: 365 HEETCRKIKALHQFDCLRANKST 387
Score = 52.8 bits (121), Expect = 2e-07
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +1
Query: 124 KIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDEND 237
++ TF IG+ SPDL A R VADYLGT HHE QF D
Sbjct: 261 QLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQD 298
Score = 44.0 bits (99), Expect = 1e-04
Identities = 26/62 (41%), Positives = 39/62 (62%), Gaps = 4/62 (6%)
Frame = +2
Query: 509 AFSLELRVPFLDIQFTHHYLSIPP--KLRQPQNGRLRKHLLRSSF--AKSGLLPDCVLWR 676
A+ +E RVPFLD +F + +SI P KL +P GR+ K +LR++F + LP +L+R
Sbjct: 389 AWGVEARVPFLDKEFLNVAMSIDPEWKLIKPDLGRIEKWVLRNAFDDEERPYLPKHILYR 448
Query: 677 HK 682
K
Sbjct: 449 QK 450
>At3g16550.1 68416.m02113 DegP protease, putative contains
similarity to DegP2 protease GI:13172275 from
[Arabidopsis thaliana]
Length = 491
Score = 28.7 bits (61), Expect = 3.9
Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 3/59 (5%)
Frame = +3
Query: 264 YHLESYDITTIRASLPM---YLLSKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEK 431
Y+L SY I +P+ Y+ KY K + V+ S D++ +GY F+D EK
Sbjct: 372 YNLPSYYIFGGFVFVPLTKSYIDDKYYKITDEQHVIISQVMPDDINKGYSNFKDLQVEK 430
>At4g02870.1 68417.m00387 expressed protein
Length = 310
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +3
Query: 324 SKYIKEKTDTTVVFSGEGTDELAQGYIYFRDAPSEKDAHKESVRLLSDIY-LYDGL 488
S Y++ K S E +E+ +GY +R+ PS D +++ + +D + LYD +
Sbjct: 18 SSYVRSKEAEKNSPSQETDEEVLKGYESYRENPSNYD--NDNILIYTDTFNLYDDM 71
>At1g53020.1 68414.m06002 ubiquitin-conjugating enzyme family
protein similar to ubiquitin-conjugating enzyme
GB:3319990 from [Mus musculus]; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 1163
Score = 27.5 bits (58), Expect = 9.0
Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = +2
Query: 536 FLDIQFTHHYLSIPPKLRQPQNG-RLRKHLLR 628
F DIQF Y S+PPK+ G R+ +L +
Sbjct: 655 FFDIQFPDTYPSVPPKVHYHSGGLRINPNLYK 686
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,765,446
Number of Sequences: 28952
Number of extensions: 299735
Number of successful extensions: 727
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 686
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 721
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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