Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30641
         (602 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC613.06 |rpl902|rpl9-2|60S ribosomal protein L9|Schizosacchar...   109   2e-25
SPAC4G9.16c |rpl901|rpl9-1|60S ribosomal protein L9|Schizosaccha...   109   4e-25
SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyce...    30   0.30 
SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr...    28   0.91 
SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c...    27   1.6  
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac...    27   1.6  
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|...    26   3.7  
SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|ch...    26   3.7  
SPBC1718.05 |trs31||TRAPP complex subunit Trs31 |Schizosaccharom...    25   6.4  
SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   6.4  
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large...    25   8.5  
SPBPB2B2.13 |||galactokinase Gal1 |Schizosaccharomyces pombe|chr...    25   8.5  
SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces po...    25   8.5  

>SPCC613.06 |rpl902|rpl9-2|60S ribosomal protein
           L9|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 189

 Score =  109 bits (263), Expect = 2e-25
 Identities = 53/86 (61%), Positives = 65/86 (75%)
 Frame = +2

Query: 254 TKGFQYKMRAVYAHFPINCVTTEGNSIIEIRNFLGEKYIRRVKMAPGVTVVNSPKQKDEL 433
           T+GF+YKMR VYAHFPIN   TE  +++EIRNFLGE+  R +K  PGVTV  S   KDE+
Sbjct: 83  TQGFRYKMRLVYAHFPININLTENGTVVEIRNFLGERITRVIKCLPGVTVSISSAVKDEI 142

Query: 434 IIEGNSLEDVSSSAALIQQSTTVKNR 511
           IIEGNSLE+VS SAA I+Q   V+N+
Sbjct: 143 IIEGNSLENVSQSAANIKQICNVRNK 168



 Score = 74.5 bits (175), Expect = 1e-14
 Identities = 36/101 (35%), Positives = 61/101 (60%)
 Frame = +3

Query: 12  KQIVANQKVKIPDGLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNPRLLKVEKWFGS 191
           + I  ++ + IP+G++V +K+RLVTVKGPRGVLK+N + + ++++      +K   W GS
Sbjct: 3   RDIYKDETLTIPEGVSVDIKARLVTVKGPRGVLKQNLRRVDIELKK-QGNTIKFIVWHGS 61

Query: 192 KKELAAVRTVCSHVENMIKGVLKASNTRCVLCMLTSPLTVS 314
           +K  A +RT  S + NMI GV +    +  L     P+ ++
Sbjct: 62  RKHNACIRTAYSIINNMIIGVTQGFRYKMRLVYAHFPININ 102



 Score = 31.1 bits (67), Expect = 0.13
 Identities = 12/17 (70%), Positives = 15/17 (88%)
 Frame = +1

Query: 511 DIRKFLDGLYVSEKTTV 561
           DIRKFLDG+YVSE+  +
Sbjct: 169 DIRKFLDGIYVSERGNI 185


>SPAC4G9.16c |rpl901|rpl9-1|60S ribosomal protein
           L9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score =  109 bits (261), Expect = 4e-25
 Identities = 52/86 (60%), Positives = 65/86 (75%)
 Frame = +2

Query: 254 TKGFQYKMRAVYAHFPINCVTTEGNSIIEIRNFLGEKYIRRVKMAPGVTVVNSPKQKDEL 433
           T+GF+YKMR VYAHFPIN   TE  +++EIRNFLGE+  R +K  PGVTV  S   KDE+
Sbjct: 83  TQGFRYKMRLVYAHFPININLTENGTVVEIRNFLGERITRVIKCLPGVTVSISSAVKDEI 142

Query: 434 IIEGNSLEDVSSSAALIQQSTTVKNR 511
           I+EGNSLE+VS SAA I+Q   V+N+
Sbjct: 143 ILEGNSLENVSQSAANIKQICNVRNK 168



 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 35/101 (34%), Positives = 59/101 (58%)
 Frame = +3

Query: 12  KQIVANQKVKIPDGLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNPRLLKVEKWFGS 191
           + I  ++ + IP G+TV +K+R VTV GPRG LK+N +H+ ++++      +K   W GS
Sbjct: 3   RDIYKDETLTIPKGVTVDIKARNVTVTGPRGTLKQNLRHVDIEMKK-QGNTIKFIVWHGS 61

Query: 192 KKELAAVRTVCSHVENMIKGVLKASNTRCVLCMLTSPLTVS 314
           +K  A +R+V S + NMI GV +    +  L     P+ ++
Sbjct: 62  RKHNACIRSVYSIINNMIIGVTQGFRYKMRLVYAHFPININ 102



 Score = 31.1 bits (67), Expect = 0.13
 Identities = 12/17 (70%), Positives = 15/17 (88%)
 Frame = +1

Query: 511 DIRKFLDGLYVSEKTTV 561
           DIRKFLDG+YVSE+  +
Sbjct: 169 DIRKFLDGIYVSERGNI 185


>SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1004

 Score = 29.9 bits (64), Expect = 0.30
 Identities = 25/89 (28%), Positives = 41/89 (46%), Gaps = 1/89 (1%)
 Frame = +3

Query: 15  QIVANQKVKIPDGLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNPRLLKVEKW-FGS 191
           ++  NQ    P G    V  R  T  G R +L+R  +   VD   +  RL  VE+  F S
Sbjct: 413 ELFVNQTDHTPVGSLFWVLDRTYTRFGQR-MLQRWLQKPLVDKENIIERLDAVEELAFNS 471

Query: 192 KKELAAVRTVCSHVENMIKGVLKASNTRC 278
             ++ A+R +   + ++ KG+ +    RC
Sbjct: 472 NSQVQAIRKMLYRLPDLEKGLSRIYYQRC 500


>SPAC6C3.04 |cit1||citrate synthase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 473

 Score = 28.3 bits (60), Expect = 0.91
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = -2

Query: 328 ITLSGDTVNGEVSIHSTHLVLEAFSTPLI 242
           +T+  D   G VS H+ HLV  A S+P +
Sbjct: 268 LTIHADHEGGNVSAHTGHLVGSALSSPFL 296


>SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1717

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 4/73 (5%)
 Frame = +3

Query: 39   KIPDGLTVHVKSRLVTV---KGP-RGVLKRNFKHLAVDIRMVNPRLLKVEKWFGSKKELA 206
            K+PDG+ +   SRL      K P    L   F H +VD+ +    L + E WF       
Sbjct: 1564 KVPDGIFLKT-SRLPIFEANKAPYNAYLLDFFTHGSVDMLIEQNNLKQSEIWFVLNDFSL 1622

Query: 207  AVRTVCSHVENMI 245
             + T+CS + N++
Sbjct: 1623 VLATICSCLGNLL 1635


>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
           Smc6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1140

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 14/50 (28%), Positives = 27/50 (54%)
 Frame = +2

Query: 407 NSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNRISESSWMVFMYLRKQ 556
           +SPK+K +L ++G  L+ +  + +LI+QS      +  +      YL K+
Sbjct: 249 SSPKEKYQLFMKGIQLKQLEENYSLIEQSLINTKNVLGNKKTGVSYLAKK 298


>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1184

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 19/67 (28%), Positives = 29/67 (43%)
 Frame = -1

Query: 293 EHTQHASCIGSL*YSFNHVLYM*TDCPHGGKLLFGSEPFLNLQETRVYHANVNSQVFEVP 114
           EHT + +   ++ Y  +         PH GK    S   +N  E+R   A+V+S   + P
Sbjct: 101 EHTDNVTTSQTIRYETSSQPSQEVYHPHAGKAA-QSAHVMNSTESRPSSAHVSSSYTQKP 159

Query: 113 FENSAGP 93
           F    GP
Sbjct: 160 FAFPLGP 166


>SPAC17H9.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 604

 Score = 26.2 bits (55), Expect = 3.7
 Identities = 13/49 (26%), Positives = 22/49 (44%)
 Frame = -1

Query: 221 DCPHGGKLLFGSEPFLNLQETRVYHANVNSQVFEVPFENSAGPFNCHQT 75
           D P+G     GS  F+      V+H+N N+   ++  +N     N H +
Sbjct: 386 DFPYGNSYGNGSSHFIANYGGSVHHSNENTMQSDLQHQNGNNAVNHHHS 434


>SPBC1718.05 |trs31||TRAPP complex subunit Trs31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 209

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 13/57 (22%), Positives = 29/57 (50%)
 Frame = +3

Query: 171 VEKWFGSKKELAAVRTVCSHVENMIKGVLKASNTRCVLCMLTSPLTVSPLRVIQLLR 341
           + K+    KE+  +   C+++  +I+G L ++   C     + PL+  P R + L++
Sbjct: 141 LNKFISVPKEMNQLNC-CAYLAGIIEGFLDSAQFPCKASAHSVPLSQYPYRTVILIK 196


>SPBC582.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 601

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = -2

Query: 205 ASSFLDPNHFSTFRRRGFTMRMSTAKC-LKFLLRTPRG 95
           A SFL+P   STF R  F+  +S   C +   LR+P G
Sbjct: 341 AQSFLEPQTRSTFLRYLFSDEVSVKVCHVLKELRSPTG 378


>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
            subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1405

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = +2

Query: 398  TVVNSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNRISE 520
            +V+N    K EL +EG  L+ V ++  ++   T+  N + E
Sbjct: 1228 SVINQESGKIELFMEGTGLQAVMNTEGIVGTKTST-NHVME 1267


>SPBPB2B2.13 |||galactokinase Gal1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 519

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 17/37 (45%), Positives = 23/37 (62%), Gaps = 2/37 (5%)
 Frame = -2

Query: 559 QLFSQIHKDH--PRTF*YPILDCSRLLNESSRARDIF 455
           +LF Q HKD   P+ F   +L   RLLN+S R+ DI+
Sbjct: 399 KLFHQ-HKDSDDPQKF---MLAFGRLLNDSQRSEDIY 431


>SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 427

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 14/42 (33%), Positives = 22/42 (52%)
 Frame = +2

Query: 395 VTVVNSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNRISE 520
           V +  SP  KDEL ++ +   + SS   LIQQ      +++E
Sbjct: 83  VNINVSPLVKDELHLKRDFCSEASSIKFLIQQGFDFNKQLTE 124


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,548,172
Number of Sequences: 5004
Number of extensions: 52993
Number of successful extensions: 158
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)