BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30636 (762 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 78 1e-15 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 59 8e-10 SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 46 8e-06 SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 33 0.044 SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 27 2.9 SPCC1393.09c |||RWD domain|Schizosaccharomyces pombe|chr 3|||Manual 26 5.1 SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex ... 26 5.1 SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 26 6.7 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 26 6.7 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 22 8.7 SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 25 8.9 SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces pom... 25 8.9 SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 25 8.9 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 8.9 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 78.2 bits (184), Expect = 1e-15 Identities = 47/146 (32%), Positives = 74/146 (50%) Frame = +3 Query: 294 ISIHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEM 473 I IH V L+A+ K + ++ FL ++ D F DYF + E I++N+V+VYELLDE+ Sbjct: 58 IYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEI 117 Query: 474 LDNGFPLATESKY*KN*LNHQIF*EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYAN 653 +D GFP TE+K Q + Q ++++ P+ R G+ Y Sbjct: 118 MDFGFPQTTETKI------LQEYITQTSNTVKKHAPPPIAM----TNAISWRSEGIHYRK 167 Query: 654 NEAYFDVVEEVDAIIDKSGATVNAGI 731 NE + DV+E V+ I G + + I Sbjct: 168 NEVFLDVIESVNLIAAADGTVIQSEI 193 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 58.8 bits (136), Expect = 8e-10 Identities = 42/160 (26%), Positives = 72/160 (45%), Gaps = 3/160 (1%) Frame = +3 Query: 261 PPVLAAPHHYLISIHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKEN 440 P V Y+ + H + +VA+ K + V+EFL ++ YF E +K+N Sbjct: 45 PIVSIGSSTYIYTKHED-LYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDN 103 Query: 441 YVVVYELLDEMLDNGFPLATESKY*KN*LNHQIF*EQLPIL-LQENQMYPLHFRLDS--C 611 ++ELLDEM+D G TE ++ ++ L L+ + L S Sbjct: 104 VSFIFELLDEMIDYGIIQTTEPDALARSVSITAVKKKGNALSLKRSHSSQLAHTTSSEIP 163 Query: 612 QMYLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVNAGI 731 RR+G+KY N Y D+VE ++ +I +G + + + Sbjct: 164 GSVPWRRAGIKYRKNSIYIDIVERMNLLISSTGNVLRSDV 203 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 133 MIHSLFIINPSGDVFLEKHWRSVIPRSVCDYY 228 MI LFI N GD + K +R + +SV + + Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIF 32 >SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 45.6 bits (103), Expect = 8e-06 Identities = 30/123 (24%), Positives = 57/123 (46%) Frame = +3 Query: 342 EVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLDNGFPLATESKY*KN 521 + PL++ + + R+VD + +F + +++N V+ +LL EM+D G+ E ++ Sbjct: 76 DTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYATCMEPNALQD 135 Query: 522 *LNHQIF*EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYANNEAYFDVVEEVDAIID 701 + F + + P R D+ R + KYA NE + V+E V A+ Sbjct: 136 IVPLPSFMNKFMAVTGLQTNTPTLAR-DTVPW---RTAKAKYATNEFFIHVLERVSAVYQ 191 Query: 702 KSG 710 +G Sbjct: 192 PNG 194 >SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 33.1 bits (72), Expect = 0.044 Identities = 16/61 (26%), Positives = 33/61 (54%), Gaps = 2/61 (3%) Frame = +3 Query: 300 IHRGGVALVAVC--KQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEM 473 ++R +L VC +Q+ L ++E +H+ V+ YF + E + N+ Y +++E+ Sbjct: 59 VYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFEKAYYVMEEL 118 Query: 474 L 476 L Sbjct: 119 L 119 >SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharomyces pombe|chr 3|||Manual Length = 730 Score = 27.1 bits (57), Expect = 2.9 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +3 Query: 381 VVDTFQDYFSDCTETII 431 +VDTF+DY DC E ++ Sbjct: 562 IVDTFRDYIVDCIEHMV 578 >SPCC1393.09c |||RWD domain|Schizosaccharomyces pombe|chr 3|||Manual Length = 215 Score = 26.2 bits (55), Expect = 5.1 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = -3 Query: 127 RQILSSIFSSQFKCFNLAT 71 R+IL SI+ +FKC N +T Sbjct: 9 REILESIYPEEFKCINDST 27 >SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex subunit Apc3|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 26.2 bits (55), Expect = 5.1 Identities = 11/43 (25%), Positives = 23/43 (53%) Frame = +3 Query: 354 LFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLDN 482 +F E LH + D+ + + + +Y +VY+LLD ++ + Sbjct: 22 IFYSERLHAIEDSNESLYLLAYSHFLNLDYNIVYDLLDRVISH 64 >SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 25.8 bits (54), Expect = 6.7 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = -1 Query: 567 VTVLAIVLKIFGGLINSFNIYFPWPVGSHYLTFHPII 457 V V+A V+ +F GL+NS + + + Y TFH I+ Sbjct: 190 VGVMAAVI-VFHGLVNSLSTRWLDRITRFYATFHLIV 225 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = -1 Query: 609 SCPDGSVEDTFDFPVTVLAIVLKIFGGLI 523 SCP G+V D F + KI GLI Sbjct: 511 SCPSGNVNDLMRFETEKARVEAKIVEGLI 539 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 21.8 bits (44), Expect(2) = 8.7 Identities = 7/13 (53%), Positives = 11/13 (84%) Frame = -1 Query: 51 HLVKFTFGDWHRD 13 H+V+FT G+ +RD Sbjct: 822 HVVRFTLGELNRD 834 Score = 21.4 bits (43), Expect(2) = 8.7 Identities = 9/15 (60%), Positives = 10/15 (66%) Frame = -1 Query: 204 DHTSPMLL*KHVARW 160 DHTS LL HV R+ Sbjct: 812 DHTSESLLYNHVVRF 826 >SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 707 Score = 25.4 bits (53), Expect = 8.9 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +3 Query: 573 NQMYPLHFRLDSCQMYLGRRSGVKYANNEAYFDVVE 680 +Q++P+ F + +YL R +GV + F+V++ Sbjct: 481 SQLFPMRFHIIRSLIYLSRHTGVFIPLAPSLFEVLD 516 >SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 470 Score = 25.4 bits (53), Expect = 8.9 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = +3 Query: 384 VDTFQDYFSDCTETIIKENYVVVYELLDEMLDNGF 488 +D Y CTE+ + VV+Y +L+ NGF Sbjct: 311 IDRITRYAEVCTESPSGVSQVVLYAILNRWGQNGF 345 >SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 25.4 bits (53), Expect = 8.9 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = +1 Query: 106 KLNLESVENMIHSLFIINPSGDVFLEKHWRSVIPRS 213 K L+S+ + SLFI NP G + WR I S Sbjct: 21 KTRLKSLYSDFTSLFIKNPEGFLANVNTWREAIETS 56 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 8.9 Identities = 11/33 (33%), Positives = 21/33 (63%) Frame = -2 Query: 716 GSSTFVNNGIDFFNYIKVSFIVSILYT*PSPKV 618 G+ + +N I+FFNY+ + S++Y+ P+V Sbjct: 297 GNVVYPDN-INFFNYVDYLLVPSLVYSMEFPRV 328 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,178,255 Number of Sequences: 5004 Number of extensions: 66361 Number of successful extensions: 175 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 175 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 365309308 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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