Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30636
(762 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun... 116 1e-26
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 80 2e-15
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 79 4e-15
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 57 1e-08
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 57 1e-08
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun... 51 1e-06
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun... 51 1e-06
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 32 0.48
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 31 0.63
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 30 1.5
At5g09320.1 68418.m01080 vacuolar sorting protein 9 domain-conta... 28 5.9
At1g33290.2 68414.m04118 sporulation protein-related isoform con... 28 5.9
At1g33290.1 68414.m04117 sporulation protein-related isoform con... 28 5.9
At1g10095.1 68414.m01138 protein prenyltransferase alpha subunit... 28 5.9
>At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 415
Score = 116 bits (280), Expect = 1e-26
Identities = 64/159 (40%), Positives = 91/159 (57%), Gaps = 3/159 (1%)
Frame = +3
Query: 264 PVLAAPHHYLISIHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENY 443
PV+A+P HYL I R G+ +A + E+ PL IEFL RV D +Y E +IK+N+
Sbjct: 48 PVIASPTHYLFQIVRDGITFLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNF 107
Query: 444 VVVYELLDEMLDNGFPLATESKY*KN*L-NHQIF*EQLPILL--QENQMYPLHFRLDSCQ 614
++VYELLDEM+DNGFPL TE K + + + L ++ N L SC
Sbjct: 108 IIVYELLDEMIDNGFPLTTEPSILKEMIAPPNLVSKMLSVVTGNASNVSDTLPSGAGSCV 167
Query: 615 MYLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVNAGI 731
+ R + KY++NE Y D+VEE+DAI+++ G V I
Sbjct: 168 PW--RPTDPKYSSNEVYVDLVEEMDAIVNRDGELVKCEI 204
Score = 27.9 bits (59), Expect = 7.8
Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +1
Query: 133 MIHSLFIINPSGDVFLEKHWRS-VIPRSVCDYY 228
M+ +F+I+ SG+V LEK + RS+C ++
Sbjct: 1 MLQCIFLISDSGEVMLEKQLTGHRVDRSICAWF 33
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 79.8 bits (188), Expect = 2e-15
Identities = 43/144 (29%), Positives = 74/144 (51%)
Frame = +3
Query: 300 IHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLD 479
+ V L+ +Q ++ FLHRVVD F+ YF + E +++N+VVVYELLDEM+D
Sbjct: 63 VQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMD 122
Query: 480 NGFPLATESKY*KN*LNHQIF*EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYANNE 659
G+P TE++ + + ++ Q P+ R G++Y NE
Sbjct: 123 FGYPQYTEARILSEFIKTDAY------RMEVTQRPPMAV----TNAVSWRSEGIQYKKNE 172
Query: 660 AYFDVVEEVDAIIDKSGATVNAGI 731
+ DV+E V+ +++ +G V + +
Sbjct: 173 VFLDVIENVNILVNSNGQIVRSDV 196
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 78.6 bits (185), Expect = 4e-15
Identities = 42/144 (29%), Positives = 74/144 (51%)
Frame = +3
Query: 300 IHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLD 479
+ + L+ +Q ++ FLHRVVD F+ YF + E +++N+VVVYELLDEM+D
Sbjct: 63 VQHSNIYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELLDEMMD 122
Query: 480 NGFPLATESKY*KN*LNHQIF*EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYANNE 659
G+P TE++ + + ++ Q P+ R G+K+ NE
Sbjct: 123 FGYPQFTEARILSEFIKTDAY------RMEVTQRPPMAV----TNSVSWRSEGLKFKKNE 172
Query: 660 AYFDVVEEVDAIIDKSGATVNAGI 731
+ DV+E V+ +++ +G V + +
Sbjct: 173 VFLDVIESVNILVNSNGQIVRSDV 196
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 56.8 bits (131), Expect = 1e-08
Identities = 33/112 (29%), Positives = 56/112 (50%)
Frame = +3
Query: 255 DVPPVLAAPHHYLISIHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIK 434
+ PP+ + G+ VA + V+P V+E L R+ +DY E +
Sbjct: 47 EAPPIFNVDGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFR 106
Query: 435 ENYVVVYELLDEMLDNGFPLATESKY*KN*LNHQIF*EQLPILLQENQMYPL 590
+N+V+VYELLDE++D G+ T ++ L IF E PI++ ++ P+
Sbjct: 107 KNFVLVYELLDEVIDFGYVQTTSTEV----LKSYIFNE--PIVVSPARLQPI 152
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 56.8 bits (131), Expect = 1e-08
Identities = 33/112 (29%), Positives = 56/112 (50%)
Frame = +3
Query: 255 DVPPVLAAPHHYLISIHRGGVALVAVCKQEVAPLFVIEFLHRVVDTFQDYFSDCTETIIK 434
+ PP+ + G+ VA + V+P V+E L R+ +DY E +
Sbjct: 47 EAPPIFNVDGVNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFR 106
Query: 435 ENYVVVYELLDEMLDNGFPLATESKY*KN*LNHQIF*EQLPILLQENQMYPL 590
+N+V+VYELLDE++D G+ T ++ L IF E PI++ ++ P+
Sbjct: 107 KNFVLVYELLDEVIDFGYVQTTSTEV----LKSYIFNE--PIVVSPARLQPI 152
>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 441
Score = 50.8 bits (116), Expect = 1e-06
Identities = 32/123 (26%), Positives = 56/123 (45%), Gaps = 1/123 (0%)
Frame = +3
Query: 366 EFLHRVVDTFQDYFSDC-TETIIKENYVVVYELLDEMLDNGFPLATESKY*KN*LNHQIF 542
+F+ V F+ YF E I+ N+V++YELLDE++D G+P + K + +
Sbjct: 83 KFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDEIMDFGYPQNLSPEILKLYITQEGV 142
Query: 543 *EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVN 722
+ P + + L RR G+ Y NE + D+VE V+ ++ G +
Sbjct: 143 --RSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKNEVFLDIVESVNLLMSSKGNVLR 200
Query: 723 AGI 731
+
Sbjct: 201 CDV 203
>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 438
Score = 50.8 bits (116), Expect = 1e-06
Identities = 32/123 (26%), Positives = 56/123 (45%), Gaps = 1/123 (0%)
Frame = +3
Query: 366 EFLHRVVDTFQDYFSDC-TETIIKENYVVVYELLDEMLDNGFPLATESKY*KN*LNHQIF 542
+F+ V F+ YF E I+ N+V++YELLDE++D G+P + K + +
Sbjct: 83 KFVVEAVALFKSYFGGAFDEDAIRNNFVLIYELLDEIMDFGYPQNLSPEILKLYITQEGV 142
Query: 543 *EQLPILLQENQMYPLHFRLDSCQMYLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVN 722
+ P + + L RR G+ Y NE + D+VE V+ ++ G +
Sbjct: 143 --RSPFSSKPKDKPVPNATLQVTGAVGWRREGLAYKKNEVFLDIVESVNLLMSSKGNVLR 200
Query: 723 AGI 731
+
Sbjct: 201 CDV 203
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 31.9 bits (69), Expect = 0.48
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +3
Query: 339 QEVAPLFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLDNG 485
QE L V+E +H V+ YF E + N+ Y +LDE+L G
Sbjct: 73 QEDNELEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAG 121
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 31.5 bits (68), Expect = 0.63
Identities = 14/44 (31%), Positives = 26/44 (59%)
Frame = +3
Query: 354 LFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLDNG 485
L ++EF+H +V+T +F + E I + + +L+EM+ NG
Sbjct: 79 LAILEFIHLLVETMDKHFGNVCELDIMFHLEKAHFMLEEMVMNG 122
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 30.3 bits (65), Expect = 1.5
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +3
Query: 354 LFVIEFLHRVVDTFQDYFSDCTETIIKENYVVVYELLDEMLDNG 485
L V+E +H V+ YF E + N+ Y +LDE+L G
Sbjct: 78 LEVLEIIHHYVEILDRYFGSVCELDLIFNFHKAYYILDELLIAG 121
>At5g09320.1 68418.m01080 vacuolar sorting protein 9
domain-containing protein / VPS9 domain-containing
protein contains similarity to Rab5 GDP/GTP exchange
factor, Rabex5 [Bos taurus] gi|2558516|emb|CAA04545;
contains Pfam profile PF02204: Vacuolar sorting protein
9 (VPS9) domain
Length = 712
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/29 (58%), Positives = 18/29 (62%)
Frame = -2
Query: 686 DFFNYIKVSFIVSILYT*PSPKVHLTAVQ 600
DF IK SFIVSIL T P P+ AVQ
Sbjct: 21 DFIKSIK-SFIVSILNTAPDPEKDCDAVQ 48
>At1g33290.2 68414.m04118 sporulation protein-related isoform
contains non-consensus AT-donor acceptor site at intron
6; similar to Stage III sporulation protein AA.
(Swiss-Prot:Q01367) [Bacillus subtilis]; similar to
SpoIIIAA (GI:1303904) [Bacillus subtilis]; similar to
stage III sporulation protein AA (GI:18145497)
[Clostridium perfringens str. 13]
Length = 303
Score = 28.3 bits (60), Expect = 5.9
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 618 YLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVNAGI 731
YLG G NNE + +E+ ++ + GA AGI
Sbjct: 85 YLGEPGGQYLRNNEVSMEELEDAQELVGEFGADNRAGI 122
>At1g33290.1 68414.m04117 sporulation protein-related isoform
contains non-consensus AT-donor acceptor site at intron
6; similar to Stage III sporulation protein AA.
(Swiss-Prot:Q01367) [Bacillus subtilis]; similar to
SpoIIIAA (GI:1303904) [Bacillus subtilis]; similar to
stage III sporulation protein AA (GI:18145497)
[Clostridium perfringens str. 13]
Length = 379
Score = 28.3 bits (60), Expect = 5.9
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 618 YLGRRSGVKYANNEAYFDVVEEVDAIIDKSGATVNAGI 731
YLG G NNE + +E+ ++ + GA AGI
Sbjct: 85 YLGEPGGQYLRNNEVSMEELEDAQELVGEFGADNRAGI 122
>At1g10095.1 68414.m01138 protein prenyltransferase alpha
subunit-related contains weak hit to Pfam profile
PF01239: Protein prenyltransferase alpha subunit repeat
Length = 431
Score = 28.3 bits (60), Expect = 5.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1
Query: 259 YHRCWLLHIITSYQFIEE 312
YHRCWL+ +T Q I+E
Sbjct: 218 YHRCWLVSYMTIEQVIQE 235
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,457,645
Number of Sequences: 28952
Number of extensions: 340510
Number of successful extensions: 811
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 790
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 807
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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