BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30635 (684 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 118 1e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 117 1e-27 SPCC1223.15c |spc19||DASH complex subunit Spc19|Schizosaccharomy... 28 1.4 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 28 1.4 SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 25 7.7 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 118 bits (283), Expect = 1e-27 Identities = 50/85 (58%), Positives = 66/85 (77%) Frame = +1 Query: 1 LRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXX 180 L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+GPL++FN+D G+ +AFRNIPGVE Sbjct: 171 LKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIV 230 Query: 181 XXXXXXXXXXAPGGHLGRFVIWTQS 255 APGGHLGRFVIWT+S Sbjct: 231 NVRRLNLLQLAPGGHLGRFVIWTKS 255 Score = 72.1 bits (169), Expect = 7e-14 Identities = 38/83 (45%), Positives = 54/83 (65%), Gaps = 2/83 (2%) Frame = +3 Query: 255 AFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-T 428 AFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA Sbjct: 256 AFGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHV 315 Query: 429 RKLNPLTNNKAMLKLNPYAAVLK 497 +K NPL N + +LNPYA K Sbjct: 316 QKKNPLKNKAVLSRLNPYAKAYK 338 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 117 bits (282), Expect = 1e-27 Identities = 50/85 (58%), Positives = 66/85 (77%) Frame = +1 Query: 1 LRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXX 180 L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+GPL++FN+D G+ +AFRNIPGVE Sbjct: 171 LKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIV 230 Query: 181 XXXXXXXXXXAPGGHLGRFVIWTQS 255 APGGHLGRFVIWT+S Sbjct: 231 NVRRLNLLQLAPGGHLGRFVIWTKS 255 Score = 72.9 bits (171), Expect = 4e-14 Identities = 38/83 (45%), Positives = 54/83 (65%), Gaps = 2/83 (2%) Frame = +3 Query: 255 AFGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-T 428 AFG LD +FGS ++ KKN+ LP+ ++N D+TRL+ SDEI+ +++A RV RA Sbjct: 256 AFGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHV 315 Query: 429 RKLNPLTNNKAMLKLNPYAAVLK 497 +K NPL N + +LNPYA K Sbjct: 316 QKKNPLKNKAVLARLNPYAKAYK 338 >SPCC1223.15c |spc19||DASH complex subunit Spc19|Schizosaccharomyces pombe|chr 3|||Manual Length = 152 Score = 27.9 bits (59), Expect = 1.4 Identities = 15/50 (30%), Positives = 27/50 (54%) Frame = +2 Query: 218 EAILDVSSSGLSRIRQA*PLIRVMEDTIKTKEELQPAPTEDGQH*PHTSS 367 E + + S R+ + ++ ED++KTK ELQ A + ++ P TS+ Sbjct: 65 EIVKPILSKAFQRLEDSISSLQRQEDSLKTKYELQEARLDMLKNRPATSA 114 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 27.9 bits (59), Expect = 1.4 Identities = 19/59 (32%), Positives = 29/59 (49%) Frame = +3 Query: 303 KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNP 479 KQK + + Q K LT+ S E + VL +R T+K LTN+ ++K +P Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSP 786 >SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 25.4 bits (53), Expect = 7.7 Identities = 10/39 (25%), Positives = 20/39 (51%) Frame = -2 Query: 485 RVRIEFQHRLVISEWVQFACSTDHAFVGSTEDLPDLIRL 369 + ++ F H L + +WV+ + +G + PD +RL Sbjct: 171 KYKVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRL 209 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,533,799 Number of Sequences: 5004 Number of extensions: 47163 Number of successful extensions: 127 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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