BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30629 (728 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 122 3e-28 At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 122 3e-28 At5g44980.1 68418.m05516 F-box family protein contains F-box dom... 29 2.4 At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 29 4.2 At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 29 4.2 At3g14980.1 68416.m01894 PHD finger transcription factor, putati... 28 7.3 At5g10800.1 68418.m01255 RNA recognition motif (RRM)-containing ... 27 9.6 At4g29500.1 68417.m04209 prolidase-related contains similarity t... 27 9.6 >At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 122 bits (293), Expect = 3e-28 Identities = 52/84 (61%), Positives = 66/84 (78%) Frame = +2 Query: 2 GASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQH 181 GA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF H Sbjct: 1082 GAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSH 1141 Query: 182 LEMHMRSENSPLCGRDHLSFRSYY 253 LEMHMR E PLCGRDH+++RS Y Sbjct: 1142 LEMHMRQEYPPLCGRDHMAYRSAY 1165 >At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 122 bits (293), Expect = 3e-28 Identities = 52/84 (61%), Positives = 66/84 (78%) Frame = +2 Query: 2 GASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQH 181 GA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF H Sbjct: 1082 GAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSH 1141 Query: 182 LEMHMRSENSPLCGRDHLSFRSYY 253 LEMHMR E PLCGRDH+++RS Y Sbjct: 1142 LEMHMRQEYPPLCGRDHMAYRSAY 1165 >At5g44980.1 68418.m05516 F-box family protein contains F-box domain Pfam:PF00646 Length = 435 Score = 29.5 bits (63), Expect = 2.4 Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 7/93 (7%) Frame = +3 Query: 291 ENIDVFINLVNFKLHSDSQSADVFILKTFVLHCWYSKADELCTACDLPLW*RRLAIGCCI 470 +NI LV+ KLH+ F++ L K + +C D PL +L GC + Sbjct: 125 QNIYKSKTLVSLKLHNVELKNSDFVVSLPCLKIL--KLENICHGEDGPLVVEKLISGCSV 182 Query: 471 LES-------RVRCYQLICSFRMNSSTLSNMQI 548 LE +R ++++ R++S TL + + Sbjct: 183 LEDLELIRPFDIRTHKVLLLLRVSSQTLKSFTL 215 >At3g11960.2 68416.m01476 cleavage and polyadenylation specificity factor (CPSF) A subunit C-terminal domain-containing protein similar to Splicing factor 3B subunit 3 (Spliceosome associatedprotein 130) (SAP 130) (SF3b130) (Pre-mRNA splicing factor SF3b 130kDa subunit) (SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF A subunit region Length = 1329 Score = 28.7 bits (61), Expect = 4.2 Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%) Frame = +2 Query: 11 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 187 +KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++ Sbjct: 1180 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1238 Query: 188 -MHMRSENSPLCGRDHLSFR 244 + + +P+ G DH FR Sbjct: 1239 KLGIHPLTAPVLGNDHNEFR 1258 >At3g11960.1 68416.m01475 cleavage and polyadenylation specificity factor (CPSF) A subunit C-terminal domain-containing protein similar to Splicing factor 3B subunit 3 (Spliceosome associatedprotein 130) (SAP 130) (SF3b130) (Pre-mRNA splicing factor SF3b 130kDa subunit) (SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF A subunit region Length = 1379 Score = 28.7 bits (61), Expect = 4.2 Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%) Frame = +2 Query: 11 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 187 +KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++ Sbjct: 1230 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1288 Query: 188 -MHMRSENSPLCGRDHLSFR 244 + + +P+ G DH FR Sbjct: 1289 KLGIHPLTAPVLGNDHNEFR 1308 >At3g14980.1 68416.m01894 PHD finger transcription factor, putative contains Pfam profile: PF00628 PHD-finger Length = 1189 Score = 27.9 bits (59), Expect = 7.3 Identities = 11/39 (28%), Positives = 16/39 (41%) Frame = -3 Query: 498 ADNSEPDSQECSIRSQGGVTITADRTPCITRQPCYTNSV 382 A + +P+ C + GG I D P Q C + V Sbjct: 719 ASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQV 757 >At5g10800.1 68418.m01255 RNA recognition motif (RRM)-containing protein KIAA0332 gene, Homo sapiens, EMBL:HSAB2330 Length = 947 Score = 27.5 bits (58), Expect = 9.6 Identities = 11/26 (42%), Positives = 12/26 (46%) Frame = -3 Query: 669 TAPTAGIKAKGWWVPTPCGLTRGPTH 592 T P I G W+P P TR P H Sbjct: 397 TEPYIMIAGSGRWIPPPLPATRSPEH 422 >At4g29500.1 68417.m04209 prolidase-related contains similarity to prolidase GI:3114966 from [Suberites domuncula] Length = 169 Score = 27.5 bits (58), Expect = 9.6 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = -3 Query: 708 ANGCPHLSNRKRITAPTAGIKAKGWWVPT 622 ANGC +++N R T + A G W PT Sbjct: 140 ANGCKNMTNVPRETWEIEAVMAGGPWPPT 168 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,634,945 Number of Sequences: 28952 Number of extensions: 352278 Number of successful extensions: 809 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 793 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 809 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1594686376 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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