BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30629
(728 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 122 3e-28
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 122 3e-28
At5g44980.1 68418.m05516 F-box family protein contains F-box dom... 29 2.4
At3g11960.2 68416.m01476 cleavage and polyadenylation specificit... 29 4.2
At3g11960.1 68416.m01475 cleavage and polyadenylation specificit... 29 4.2
At3g14980.1 68416.m01894 PHD finger transcription factor, putati... 28 7.3
At5g10800.1 68418.m01255 RNA recognition motif (RRM)-containing ... 27 9.6
At4g29500.1 68417.m04209 prolidase-related contains similarity t... 27 9.6
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 122 bits (293), Expect = 3e-28
Identities = 52/84 (61%), Positives = 66/84 (78%)
Frame = +2
Query: 2 GASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQH 181
GA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF H
Sbjct: 1082 GAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSH 1141
Query: 182 LEMHMRSENSPLCGRDHLSFRSYY 253
LEMHMR E PLCGRDH+++RS Y
Sbjct: 1142 LEMHMRQEYPPLCGRDHMAYRSAY 1165
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B subunit
3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 122 bits (293), Expect = 3e-28
Identities = 52/84 (61%), Positives = 66/84 (78%)
Frame = +2
Query: 2 GASQKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQH 181
GA K D +V FHVG+ VT LQ+A++IPGGSE+++Y T+ GS+G L FTSR+D DFF H
Sbjct: 1082 GAPNKVDEIVQFHVGDVVTCLQKASMIPGGSESIMYGTVMGSIGALHAFTSRDDVDFFSH 1141
Query: 182 LEMHMRSENSPLCGRDHLSFRSYY 253
LEMHMR E PLCGRDH+++RS Y
Sbjct: 1142 LEMHMRQEYPPLCGRDHMAYRSAY 1165
>At5g44980.1 68418.m05516 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 29.5 bits (63), Expect = 2.4
Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 7/93 (7%)
Frame = +3
Query: 291 ENIDVFINLVNFKLHSDSQSADVFILKTFVLHCWYSKADELCTACDLPLW*RRLAIGCCI 470
+NI LV+ KLH+ F++ L K + +C D PL +L GC +
Sbjct: 125 QNIYKSKTLVSLKLHNVELKNSDFVVSLPCLKIL--KLENICHGEDGPLVVEKLISGCSV 182
Query: 471 LES-------RVRCYQLICSFRMNSSTLSNMQI 548
LE +R ++++ R++S TL + +
Sbjct: 183 LEDLELIRPFDIRTHKVLLLLRVSSQTLKSFTL 215
>At3g11960.2 68416.m01476 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1329
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +2
Query: 11 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 187
+KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++
Sbjct: 1180 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1238
Query: 188 -MHMRSENSPLCGRDHLSFR 244
+ + +P+ G DH FR
Sbjct: 1239 KLGIHPLTAPVLGNDHNEFR 1258
>At3g11960.1 68416.m01475 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to Splicing factor 3B subunit 3
(Spliceosome associatedprotein 130) (SAP 130) (SF3b130)
(Pre-mRNA splicing factor SF3b 130kDa subunit)
(SP:Q15393) [Homo sapiens]; contains Pfam PF03178 : CPSF
A subunit region
Length = 1379
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/80 (21%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = +2
Query: 11 QKGDVVVNFHVGETVTSLQRATLIPGGSEALLYATISGSLGVLLPFTSREDHDFFQHLE- 187
+KG + + + S + I + ++ T+ GS+ V P +S E+++ + ++
Sbjct: 1230 KKGCNIYKLPADDVLRSYGLSKSIDTADDTIIAGTLLGSIFVFAPISS-EEYELLEGVQA 1288
Query: 188 -MHMRSENSPLCGRDHLSFR 244
+ + +P+ G DH FR
Sbjct: 1289 KLGIHPLTAPVLGNDHNEFR 1308
>At3g14980.1 68416.m01894 PHD finger transcription factor, putative
contains Pfam profile: PF00628 PHD-finger
Length = 1189
Score = 27.9 bits (59), Expect = 7.3
Identities = 11/39 (28%), Positives = 16/39 (41%)
Frame = -3
Query: 498 ADNSEPDSQECSIRSQGGVTITADRTPCITRQPCYTNSV 382
A + +P+ C + GG I D P Q C + V
Sbjct: 719 ASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQV 757
>At5g10800.1 68418.m01255 RNA recognition motif (RRM)-containing
protein KIAA0332 gene, Homo sapiens, EMBL:HSAB2330
Length = 947
Score = 27.5 bits (58), Expect = 9.6
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -3
Query: 669 TAPTAGIKAKGWWVPTPCGLTRGPTH 592
T P I G W+P P TR P H
Sbjct: 397 TEPYIMIAGSGRWIPPPLPATRSPEH 422
>At4g29500.1 68417.m04209 prolidase-related contains similarity to
prolidase GI:3114966 from [Suberites domuncula]
Length = 169
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = -3
Query: 708 ANGCPHLSNRKRITAPTAGIKAKGWWVPT 622
ANGC +++N R T + A G W PT
Sbjct: 140 ANGCKNMTNVPRETWEIEAVMAGGPWPPT 168
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,634,945
Number of Sequences: 28952
Number of extensions: 352278
Number of successful extensions: 809
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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