BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30623 (789 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 128 4e-30 SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 128 7e-30 SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12) 31 1.1 SB_13066| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.7 SB_24673| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5 SB_3514| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5 SB_50016| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 9.9 SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012) 28 9.9 >SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 309 Score = 128 bits (310), Expect = 4e-30 Identities = 61/78 (78%), Positives = 70/78 (89%), Gaps = 1/78 (1%) Frame = +1 Query: 1 PTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLL 180 PT PIRLGLALNFSVF+YEI + ++AC+LAKQAFDDAIAELD+LNED YKDSTLIMQLL Sbjct: 232 PTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNEDQYKDSTLIMQLL 291 Query: 181 RDNLTLWTSDTQGD-GDE 231 RDNLTLW+S+ Q D GD+ Sbjct: 292 RDNLTLWSSENQEDQGDD 309 Score = 123 bits (297), Expect = 1e-28 Identities = 62/93 (66%), Positives = 70/93 (75%), Gaps = 2/93 (2%) Frame = +1 Query: 1 PTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLL 180 PT PIRLGLALNFSVFYYEI+ +AC LAK+AFDDAIAELDTL+ED YKDSTLIMQLL Sbjct: 143 PTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELDTLSEDQYKDSTLIMQLL 202 Query: 181 RDNLTLWTSDTQG--DGDEPAEGGDNYISPHSP 273 RDNLT+ Q + E AE GD ++P P Sbjct: 203 RDNLTVVEKALQAYKEAKEAAETGDGKLAPTDP 235 >SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) Length = 248 Score = 128 bits (308), Expect = 7e-30 Identities = 58/77 (75%), Positives = 67/77 (87%) Frame = +1 Query: 1 PTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLL 180 P+HPIRLGLALNFSVFYYEI N P +AC+LAK+AFDDAIA LD L ++SYKDSTLIMQLL Sbjct: 170 PSHPIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIMQLL 229 Query: 181 RDNLTLWTSDTQGDGDE 231 RDNLTLWTS+ +G + Sbjct: 230 RDNLTLWTSEQDQEGQD 246 >SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12) Length = 979 Score = 31.1 bits (67), Expect = 1.1 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 3/49 (6%) Frame = +1 Query: 106 DDAIAELDTLNEDSYKDSTLIMQL---LRDNLTLWTSDTQGDGDEPAEG 243 DD + D ++DS + + QL L+ + LW S TQGD D A G Sbjct: 850 DDDDFDTDEWSDDSDAEGSAAGQLKLCLKREILLWKSGTQGDADRRATG 898 >SB_13066| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 865 Score = 28.7 bits (61), Expect = 5.7 Identities = 8/15 (53%), Positives = 12/15 (80%) Frame = -2 Query: 743 IFWCQCFGYSPFKTL 699 + WC+C+GY+P TL Sbjct: 537 MIWCRCYGYTPTPTL 551 >SB_24673| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 732 Score = 28.3 bits (60), Expect = 7.5 Identities = 25/97 (25%), Positives = 39/97 (40%), Gaps = 7/97 (7%) Frame = +1 Query: 199 WTSDTQGDGDEPAEGGDNYISP------HSPQHTHLFSSVFIRKRVTFENVVWPGIITEA 360 WT T+G + PA GD SP SPQ T+ S ++ PG++ + Sbjct: 464 WTGATKGQANSPANAGDACRSPLVKQLQRSPQLTNEQPSSPTFRKAGGVCTWTPGVVGQL 523 Query: 361 DASRRL*KC*KSFAADLPVPSKIKCLCHL*RF-CISV 468 +R + + A++ + LCH R C V Sbjct: 524 HREKRPVRSLRKMASEDNTCLSSRMLCHTSRIQCYKV 560 >SB_3514| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 490 Score = 28.3 bits (60), Expect = 7.5 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = -2 Query: 419 GTGRSAANDF*HFYKRRLASASVMIPGQTTFSNVTRFLIN 300 GT R +F HF++ A ++ + G T S +TR L+N Sbjct: 194 GTSRFPEQNFDHFFQYSTAVLALCVSGYTHASFLTRPLVN 233 >SB_50016| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2065 Score = 27.9 bits (59), Expect = 9.9 Identities = 13/22 (59%), Positives = 16/22 (72%) Frame = -2 Query: 380 YKRRLASASVMIPGQTTFSNVT 315 +KR SV +PGQTTFS+VT Sbjct: 1345 WKRTQYIWSVALPGQTTFSDVT 1366 >SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012) Length = 983 Score = 27.9 bits (59), Expect = 9.9 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = +1 Query: 49 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGD 222 YY I + K Q FDD + +ED+ +D+T ++L+ T+D GD Sbjct: 505 YYNIPYNKPKKNDFKLQLFDDYKQAVTQASEDTLRDATTAIELMNAP----TTDVHGD 558 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,042,863 Number of Sequences: 59808 Number of extensions: 455605 Number of successful extensions: 1138 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1064 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1138 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2167838629 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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