Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30621
(813 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 103 2e-23
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 101 9e-23
SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces p... 29 0.79
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha... 29 1.0
SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces pombe... 27 3.2
SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 27 3.2
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 27 4.2
SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5 |Schizo... 26 5.5
SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 26 5.5
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 5.5
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 26 7.3
SPBC18H10.03 |tif35||translation initiation factor eIF3g|Schizos... 26 7.3
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 103 bits (248), Expect = 2e-23
Identities = 45/72 (62%), Positives = 58/72 (80%)
Frame = +3
Query: 288 EQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESKVFYYKMKG 467
EQKEE++G + +I+ YR ++E EL DIC D+L VL+K+LIP++ TGESKVFYYKMKG
Sbjct: 68 EQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKG 127
Query: 468 DYHRYLAEFATG 503
DY+RYLAEF G
Sbjct: 128 DYYRYLAEFTVG 139
Score = 73.7 bits (173), Expect = 3e-14
Identities = 38/55 (69%), Positives = 47/55 (85%)
Frame = +1
Query: 85 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVI 249
+ RE++VY AKLAEQAERY+EMVE MK VA S+++L+VEERNLLSVAYKN+I
Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVA---CSNDKLSVEERNLLSVAYKNII 54
Score = 50.0 bits (114), Expect = 4e-07
Identities = 23/30 (76%), Positives = 27/30 (90%)
Frame = +2
Query: 512 KEAAENSLVAYKAASDIAMTELPPTHPIRL 601
KEAA++SL AYKAASDIA+ ELPPT P+RL
Sbjct: 143 KEAADSSLEAYKAASDIAVAELPPTDPMRL 172
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 101 bits (243), Expect = 9e-23
Identities = 45/72 (62%), Positives = 59/72 (81%)
Frame = +3
Query: 288 EQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESKVFYYKMKG 467
EQKEE+KG ++ +I+ YR ++E+EL IC DIL VL+K+LIP++ + ESKVFYYKMKG
Sbjct: 69 EQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKG 128
Query: 468 DYHRYLAEFATG 503
DY+RYLAEFA G
Sbjct: 129 DYYRYLAEFAVG 140
Score = 78.2 bits (184), Expect = 1e-15
Identities = 42/55 (76%), Positives = 46/55 (83%)
Frame = +1
Query: 85 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVI 249
+ RED VY AKLAEQAERY+ MVE MK+VAS +D ELTVEERNLLSVAYKNVI
Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVI 55
Score = 47.2 bits (107), Expect = 3e-06
Identities = 29/71 (40%), Positives = 42/71 (59%)
Frame = +2
Query: 503 QRRKEAAENSLVAYKAASDIAMTELPPTHPIRLRTSRSNFFRVLLTRS*TARNRACRASP 682
++R+ +A+ SL YKAAS+IA EL PTHPIRL + + F V + +RAC +
Sbjct: 141 EKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALN--FSVFYYEILNSPDRACYLAK 198
Query: 683 KGRLSTEAIAE 715
+ EAI+E
Sbjct: 199 QA--FDEAISE 207
>SPBC11C11.08 |srp1||SR family protein Srp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 275
Score = 29.1 bits (62), Expect = 0.79
Identities = 17/42 (40%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +1
Query: 487 PSSRPATTQGGCRELAGRVQG-SLRHRHDRTAPHPPHPPSDF 609
P S P + +GG RE GRV G S R R +PH S +
Sbjct: 88 PPSGPGSKRGGRRERGGRVHGDSGRLRSRSPSPHEARSRSPY 129
>SPAC57A10.02 |cdr2||GIN4 family protein kinase
Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 28.7 bits (61), Expect = 1.0
Identities = 24/80 (30%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = +1
Query: 496 RPATTQGGCRELAGRVQGSLRHRHDRTAPHPPH-PPSDFALKLLPCSTNEILNSPEPRLP 672
RPATT +L V L + HD + + PPS L P + N +N PE +L
Sbjct: 416 RPATTSSAVPQLPKSVTPGLAYPHDSSMLSSNYRPPS----ALSPRNFNVSINDPEVQLS 471
Query: 673 GFAKRPPFYGSYRRNLDNAS 732
A +R N ++ S
Sbjct: 472 RRATSLDMSNDFRMNENDPS 491
>SPACUNK4.14 |mdb1||BRCT domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 520
Score = 27.1 bits (57), Expect = 3.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 439 DSPVCELGMRYLSRTPRMSEQMSRSSFSTW 350
DSP +G R +S+ R + RSS S+W
Sbjct: 481 DSPTKVIGKRNVSKASRTGQGRKRSSRSSW 510
>SPBC4C3.12 |sep1||fork head transcription factor
Sep1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 663
Score = 27.1 bits (57), Expect = 3.2
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +1
Query: 676 FAKRPPFYGSYRRNLDNASRREELTKEFQ 762
F P+Y ++ +NL+NAS+ EL +Q
Sbjct: 3 FNSTNPYYFTHEKNLNNASKYSELPIAYQ 31
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 26.6 bits (56), Expect = 4.2
Identities = 15/59 (25%), Positives = 28/59 (47%)
Frame = +1
Query: 73 SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVI 249
SST S N ++ Q+E Y ++ + + + ++SD E +E R V +N +
Sbjct: 45 SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSV 103
>SPAC14C4.02c |smc5|spr18|Smc5-6 complex SMC subunit Smc5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1065
Score = 26.2 bits (55), Expect = 5.5
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = +3
Query: 327 NMIRAYRSQVEKELRDICSDILGVLDK 407
N +RA + +++K+L+D+ + +LDK
Sbjct: 243 NQLRADKKKLKKDLKDLVEEFQPILDK 269
>SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 599
Score = 26.2 bits (55), Expect = 5.5
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = -3
Query: 394 PRMSEQMSRSSFSTWLRYALIMFNLSSAPFVSSFCSM 284
P ++E + +S S+W+ A ++ + + P V FC++
Sbjct: 121 PTIAEDFNDTSSSSWIGSAYVLASNAVLPAVGVFCNI 157
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 26.2 bits (55), Expect = 5.5
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = -1
Query: 762 LEFLCKFFPPRSVVQVSAIASVERRP 685
L F KF P V +SAI SV RP
Sbjct: 671 LYFKYKFLPLMDAVPISAIESVSERP 696
>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 7.3
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = -3
Query: 202 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 80
S+H + + H P F S QLTLL + YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528
>SPBC18H10.03 |tif35||translation initiation factor
eIF3g|Schizosaccharomyces pombe|chr 2|||Manual
Length = 282
Score = 25.8 bits (54), Expect = 7.3
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +3
Query: 357 EKELRDICSDILGVLDKYLIPSSQTGESKVFYY 455
E+ELRD+ G+ YL +TG +K F +
Sbjct: 215 EEELRDLFRRFGGIQRVYLAKDKETGRAKGFAF 247
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,178,165
Number of Sequences: 5004
Number of extensions: 65103
Number of successful extensions: 251
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 249
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 396433620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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